Martin C. Harmsen
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View article: Modelling cigarette smoke-induced lung vascular dysfunction using an alveolus-on-chip
Modelling cigarette smoke-induced lung vascular dysfunction using an alveolus-on-chip Open
The alveolus is central to gas exchange in the lung, and alveolar damage is a characteristics of a variety of lung diseases. Understanding alveolar microvascular dynamics and epithelial-endothelial interactions is essential for accurately …
View article: SUL-150 Limits Vascular Remodeling and Ventricular Failure in Pulmonary Arterial Hypertension
SUL-150 Limits Vascular Remodeling and Ventricular Failure in Pulmonary Arterial Hypertension Open
Pulmonary arterial hypertension (PAH) is a rare, progressive, and incurable disease characterized by an elevated pulmonary blood pressure, extensive remodeling of the pulmonary vasculature, increased pulmonary vascular resistance, and culm…
View article: Multilayered Tissue Assemblies Through Tuneable Biodegradable Polyhydroxyalkanoate Polymer (Mesh)-Reinforced Organ-Derived Extracellular Matrix Hydrogels
Multilayered Tissue Assemblies Through Tuneable Biodegradable Polyhydroxyalkanoate Polymer (Mesh)-Reinforced Organ-Derived Extracellular Matrix Hydrogels Open
Multi-layer cell constructs produced in vitro are an innovative treatment option to support the growing demand for therapy in regenerative medicine. Our research introduces a novel construct integrating organ-derived decellularised extrace…
View article: From cooperation to collapse: systemic failure in liver disease through a sociological lens
From cooperation to collapse: systemic failure in liver disease through a sociological lens Open
The liver operates as a highly coordinated microsystem, where various liver cell types engage in dynamic interactions to maintain homeostasis. This intercellular cooperation resembles sociological models of sustainable cooperation, encompa…
View article: Extracellular vesicle-dependent crosstalk between hepatic stellate cells and Kupffer cells promotes their mutual activation
Extracellular vesicle-dependent crosstalk between hepatic stellate cells and Kupffer cells promotes their mutual activation Open
In co-culture, KCs and HSCs show mutual activation in a TLR4-dependent fashion. This bidirectional activation is augmented by pro-inflammatory mediators. KC-derived EVs (partially) activate HSCs, which might contribute to progression of li…
View article: H3K27Me3 abundance is associated with a decreased barrier function of endothelial cells by directly silencing VE-cadherin expression
H3K27Me3 abundance is associated with a decreased barrier function of endothelial cells by directly silencing VE-cadherin expression Open
Decreased H3K27Me3 abundance in ECs is beneficial for the formation and integrity of the EC barrier.
View article: Development of an in vitro platform for epithelial-stromal interactions: A basement membrane-containing scaffold from decellularized porcine bladders
Development of an in vitro platform for epithelial-stromal interactions: A basement membrane-containing scaffold from decellularized porcine bladders Open
The first organized extracellular matrix that appears during mammalian embryogenesis is a basement membrane (BM), BM is present in all adult epithelia, endothelia, muscle, nerve and fat tissues. BM is a sub-micrometer thick compact lattice…
View article: Scaffold-Free Strategies in Dental Pulp/Dentine Tissue Engineering: Current Status and Implications for Regenerative Biological Processes
Scaffold-Free Strategies in Dental Pulp/Dentine Tissue Engineering: Current Status and Implications for Regenerative Biological Processes Open
Conventionally, root canal treatment is performed when the dental pulp is severely damaged or lost due to dental trauma or bacterial endodontic infections. This treatment involves removing the compromised or infected pulp tissue, disinfect…
View article: Intrapericardial injection of hydrogels with ASC and their secretome to treat dilated cardiomyopathies
Intrapericardial injection of hydrogels with ASC and their secretome to treat dilated cardiomyopathies Open
View article: Piezo1 Regulates Remodeling of Skin-Derived Extracellular Matrix by Embedded Umbilical Cord Mesenchymal Stem Cells in a Stiffness-Dependent Fashion
Piezo1 Regulates Remodeling of Skin-Derived Extracellular Matrix by Embedded Umbilical Cord Mesenchymal Stem Cells in a Stiffness-Dependent Fashion Open
View article: A Versatile Skin-Derived Extracellular Matrix Hydrogel-Based Platform to Investigate the Function of a Mechanically Isolated Adipose Tissue Stromal Vascular Fraction
A Versatile Skin-Derived Extracellular Matrix Hydrogel-Based Platform to Investigate the Function of a Mechanically Isolated Adipose Tissue Stromal Vascular Fraction Open
Introduction: To accelerate cutaneous wound healing and prevent scarring, regenerative approaches such as injecting a mechanically derived tissue stromal vascular fraction (tSVF) are currently under clinical and laboratory investigations. …
View article: Comparing mechanical and enzymatic isolation procedures to isolate adipose‐derived stromal vascular fraction: A systematic review
Comparing mechanical and enzymatic isolation procedures to isolate adipose‐derived stromal vascular fraction: A systematic review Open
The stromal vascular fraction of adipose tissue has gained popularity as regenerative therapy for tissue repair. Both enzymatic and mechanical intraoperative SVF isolation procedures exist. To date, the quest for the preferred isolation pr…
View article: Effects of Nanofat in Plastic and Reconstructive Surgery: A Systematic Review
Effects of Nanofat in Plastic and Reconstructive Surgery: A Systematic Review Open
View article: Therapeutic Efficacy of Adipose Tissue-Derived Components in Neuropathic Pain: A Systematic Review
Therapeutic Efficacy of Adipose Tissue-Derived Components in Neuropathic Pain: A Systematic Review Open
Background: Neuropathic pain results from a defect in the somatosensory nervous system caused by a diversity of etiologies. The effect of current treat-ment with analgesics and surgery is limited. Studies report the therapeutic use of adip…
View article: A 3D Epithelial–Mesenchymal Co-Culture Model of the Airway Wall Using Native Lung Extracellular Matrix
A 3D Epithelial–Mesenchymal Co-Culture Model of the Airway Wall Using Native Lung Extracellular Matrix Open
Chronic obstructive pulmonary disease (COPD) is a chronic lung disease characterized by ongoing inflammation, impaired tissue repair, and aberrant interplay between airway epithelium and fibroblasts, resulting in an altered extracellular m…
View article: Collagen-rich liver-derived extracellular matrix hydrogels augment survival and function of primary rat liver sinusoidal endothelial cells and hepatocytes
Collagen-rich liver-derived extracellular matrix hydrogels augment survival and function of primary rat liver sinusoidal endothelial cells and hepatocytes Open
View article: The role of extracellular matrix hydrogels and adipose-derived stromal cells in soft tissue vascularization – A systematic review
The role of extracellular matrix hydrogels and adipose-derived stromal cells in soft tissue vascularization – A systematic review Open
View article: Extracellular Vesicles from Adipose Tissue-Derived Stromal Cells Stimulate Angiogenesis in a Scaffold-Dependent Fashion
Extracellular Vesicles from Adipose Tissue-Derived Stromal Cells Stimulate Angiogenesis in a Scaffold-Dependent Fashion Open
View article: Physical Properties and Biochemical Composition of Extracellular Matrix-Derived Hydrogels Dictate Vascularization Potential in an Organ-Dependent Fashion
Physical Properties and Biochemical Composition of Extracellular Matrix-Derived Hydrogels Dictate Vascularization Potential in an Organ-Dependent Fashion Open
The inherent extracellular matrix (ECM) originating from a specific tissue impacts the process of vascularization, specifically vascular network formation (VNF) orchestrated by endothelial cells (ECs). The specific contribution toward thes…
View article: Fibroblast alignment and matrix remodeling induced by a stiffness gradient in a skin-derived extracellular matrix hydrogel
Fibroblast alignment and matrix remodeling induced by a stiffness gradient in a skin-derived extracellular matrix hydrogel Open
Large skin injuries heal as scars. Stiffness gradually increases from normal skin to scar tissue (20x higher), due to excessive deposition and crosslinking of extracellular matrix (ECM) mostly produced by (myo)fibroblasts. Using a custom m…
View article: H3K27Me3 abundance increases fibrogenesis during endothelial-to-mesenchymal transition via the silencing of microRNA-29c
H3K27Me3 abundance increases fibrogenesis during endothelial-to-mesenchymal transition via the silencing of microRNA-29c Open
Objective Endothelial-to-mesenchymal transition (EndMT) is a transdifferentiation process in which endothelial cells (ECs) adopt a mesenchymal-like phenotype. Over the past few years, it became clear that EndMT can contribute to several ca…
View article: Regenerative treatments for scleroderma in cutaneous manifestations of the face: a systematic review
Regenerative treatments for scleroderma in cutaneous manifestations of the face: a systematic review Open
Autologous regenerative treatments hold potential for alleviating cutaneous manifestations of the face in scleroderma. Further clinical trials should be well-designed to improve the quality of clinical evidence.
View article: Hyperglycemia amplifies TLR-mediated inflammatory response of M(IL4) macrophages to dyslipidemic ligands
Hyperglycemia amplifies TLR-mediated inflammatory response of M(IL4) macrophages to dyslipidemic ligands Open
Hyperglycemia is critical for initiation of diabetic vascular complications. We systemically addressed the role of hyperglycemia in the regulation of TLRs in primary human macrophages. Expression of TLRs (1–9) was examined in monocyte-deri…
View article: Intra-Articular Injection of Adipose-Derived Stromal Vascular Fraction in Osteoarthritic Temporomandibular Joints: Study Design of a Randomized Controlled Clinical Trial
Intra-Articular Injection of Adipose-Derived Stromal Vascular Fraction in Osteoarthritic Temporomandibular Joints: Study Design of a Randomized Controlled Clinical Trial Open
Introduction: Temporomandibular joint (TMJ) osteoarthritis is a degenerative disease of the TMJ. It is characterized by progressive degradation of the extracellular matrix components of articular cartilage, with secondary inflammatory comp…
View article: Extracellular vesicles derived from liver sinusoidal endothelial cells inhibit the activation of hepatic stellate cells and Kupffer cells in vitro
Extracellular vesicles derived from liver sinusoidal endothelial cells inhibit the activation of hepatic stellate cells and Kupffer cells in vitro Open
Liver sinusoidal endothelial cells (LSECs) play a crucial role in maintaining liver microcirculation and exchange of nutrients in the liver and are thought to be involved in the pathogenesis of metabolic dysfunction-associated steatotic li…
View article: Angiogenesis is promoted by hypoxic cervical carcinoma-derived extracellular vesicles depending on the endothelial cell environment
Angiogenesis is promoted by hypoxic cervical carcinoma-derived extracellular vesicles depending on the endothelial cell environment Open
Hypoxia differentially affects CA-EVs in a cell line-dependent manner. The cellular environment (nutrient availability and extracellular matrix scaffold) influences the effect of CA-EV on the angiogenic potential of ECs.
View article: Fibroblast Alignment and Matrix Remodeling Induced by a Stiffness Gradient in Skin-Derived Extracellular Matrix Hydrogel
Fibroblast Alignment and Matrix Remodeling Induced by a Stiffness Gradient in Skin-Derived Extracellular Matrix Hydrogel Open
View article: Organ-Derived Extracellular Matrix (ECM) Hydrogels: Versatile Systems to Investigate the Impact of Biomechanics and Biochemistry on Cells in Disease Pathology
Organ-Derived Extracellular Matrix (ECM) Hydrogels: Versatile Systems to Investigate the Impact of Biomechanics and Biochemistry on Cells in Disease Pathology Open
View article: 3-D culture of human lung fibroblasts decreases proliferative and increases extracellular matrix remodeling genes
3-D culture of human lung fibroblasts decreases proliferative and increases extracellular matrix remodeling genes Open
With the introduction of complex three-dimensional (3-D) lung models, comes a need for understanding cellular behavior in these models. We compared gene expression profiles of human lung fibroblasts grown on two-dimensional (2-D) collagen …
View article: Fibroblasts alter the physical properties of dermal ECM-derived hydrogels to create a pro-angiogenic microenvironment
Fibroblasts alter the physical properties of dermal ECM-derived hydrogels to create a pro-angiogenic microenvironment Open
This study aimed to investigate the impact of fibroblasts (MRC-5) on the extracellular matrix (ECM) microenvironment of endothelial cells (ECs) during the vascularization of skin-derived ECM hydrogel in vitro. Two types of ECs were studied…