Masahiro Takinoue
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View article: Investigating the effects of soil microstructures on bacterial growth via microfluidic channels and an agent-based model
Investigating the effects of soil microstructures on bacterial growth via microfluidic channels and an agent-based model Open
View article: Antibody‐Functionalized DNA Hydrogels Recognize and Isolate Living Tumor Cells
Antibody‐Functionalized DNA Hydrogels Recognize and Isolate Living Tumor Cells Open
DNA nanostar hydrogels are a novel class of biocompatible and programmable materials with promising applications as a cell matrix for tissue engineering or cancer diagnosis. Yet, precise isolation of specific cell types with these hydrogel…
View article: A platform for the formation of uniform DNA condensate droplets using vibration-induced local vortices
A platform for the formation of uniform DNA condensate droplets using vibration-induced local vortices Open
DNA condensate droplets (hereafter referred to as DNA condensates), which arise from specific interactions between sticky ends embedded in multi-arm DNA nanostructures, hold significant promise as programmable smart materials. However, fro…
View article: Controlled Formation of DNA Condensates as Model Nuclei in Monodisperse Giant Vesicles
Controlled Formation of DNA Condensates as Model Nuclei in Monodisperse Giant Vesicles Open
Several studies have attempted to replicate the complex hierarchy of eukaryotic cells for the bottom-up construction of artificial cells. Specifically, reconstruction of liquid-liquid phase separation systems as membrane-less organelles is…
View article: DNA Condensates via Entanglement of String-like Structures Based on Anisotropic Nanotetrahedra
DNA Condensates via Entanglement of String-like Structures Based on Anisotropic Nanotetrahedra Open
Biomolecular condensates are attracting attention for their bioinspired functionalities and potential applications. However, the influence of biomolecular structural properties on the complex phase behaviors of biomolecular condensates rem…
View article: Remote-controlled mechanical and directional motions of photoswitchable DNA condensates
Remote-controlled mechanical and directional motions of photoswitchable DNA condensates Open
Membrane-free synthetic DNA-based condensates enable programmable control of dynamic behaviors as shown by phase-separated condensates in biological cells. We demonstrate remote-controlled microflow using photocontrollable state transition…
View article: Synthetic nucleic-acid droplets: a bioprogramming platform for designer microliquids
Synthetic nucleic-acid droplets: a bioprogramming platform for designer microliquids Open
Research on biomolecular liquid-state condensates (droplets) in cells has sparked burgeoning interest among synthetic biologists in programmable droplets assembled from synthetic nucleic acids––information-encoding biomolecules amenable to…
View article: Controlled Formation of DNA Condensates as Artificial Nuclei in Monodisperse Giant Vesicles
Controlled Formation of DNA Condensates as Artificial Nuclei in Monodisperse Giant Vesicles Open
Several studies have attempted to replicate the complex hierarchy of eukaryotic cells for the bottom-up construction of artificial cells. Specifically, reconstruction of liquid–liquid phase separation systems as membrane-less organelles is…
View article: Formation dynamics of patchy/Janus DNA condensates in monodisperse giant vesicles generated using microfluidics
Formation dynamics of patchy/Janus DNA condensates in monodisperse giant vesicles generated using microfluidics Open
The one-step formation of uniform patchy or Janus-type DNA condensates within monodisperse giant vesicles was demonstrated for homogeneous production of artificial cells with hierarchical structures.
View article: A platform for the formation of uniform DNA condensate droplets using vibration-induced local vortices
A platform for the formation of uniform DNA condensate droplets using vibration-induced local vortices Open
DNA condensate droplets (hereafter referred to as DNA condensates), which arise from specific interactions between sticky ends embedded in multi-arm DNA nanostructures, hold significant promise as programmable smart materials. However,...
View article: High-speed Intracellular Temperature Mapping Reveals the Existence of Non-Conductive Dissipation of Energy by Heating
High-speed Intracellular Temperature Mapping Reveals the Existence of Non-Conductive Dissipation of Energy by Heating Open
Recently, intracellular thermometry has revealed temperature variations within cells. Although the biological significance of intracellular temperature change is recognized, the physical principles of intracellular temperature change remai…
View article: Temporally controlled multistep division of DNA droplets for dynamic artificial cells
Temporally controlled multistep division of DNA droplets for dynamic artificial cells Open
Synthetic droplets mimicking bio-soft matter droplets formed via liquid-liquid phase separation (LLPS) in living cells have recently been employed in nanobiotechnology for artificial cells, molecular robotics, molecular computing, etc. Tem…
View article: DNA‐Origami‐Armored DNA Condensates
DNA‐Origami‐Armored DNA Condensates Open
DNA condensates, formed by liquid–liquid phase separation (LLPS), emerge as promising soft matter assemblies for creating artificial cells. The advantages of DNA condensates are their molecular permeability through the surface due to their…
View article: Programmable Computational RNA Droplets Assembled via Kissing-Loop Interaction
Programmable Computational RNA Droplets Assembled via Kissing-Loop Interaction Open
DNA droplets, artificial liquid-like condensates of well-engineered DNA sequences, allow the critical aspects of phase-separated biological condensates to be harnessed programmably, such as molecular sensing and phase-state regulation. In …
View article: Preface: Special Issue on Molecular Cybernetics: Theory and Practice Toward Chemical AI
Preface: Special Issue on Molecular Cybernetics: Theory and Practice Toward Chemical AI Open
View article: Liquid DNA Coacervates form Porous Capsular Hydrogels via Viscoelastic Phase Separation on Microdroplet Interface (Adv. Mater. Interfaces 13/2024)
Liquid DNA Coacervates form Porous Capsular Hydrogels via Viscoelastic Phase Separation on Microdroplet Interface (Adv. Mater. Interfaces 13/2024) Open
Porous DNA Coacervate Capsules In article 2300898, Miho Yanagisawa, Masahiro Takinoue, and co-workers present porous capsular DNA hydrogels formed by viscoelastic phase separation of liquid-like DNA condensates on a water-in-oil microdropl…
View article: Scanning electrochemical microscopy for determining oxygen consumption rates of cells in hydrogel fibers fabricated using an extrusion 3D bioprinter
Scanning electrochemical microscopy for determining oxygen consumption rates of cells in hydrogel fibers fabricated using an extrusion 3D bioprinter Open
Three-dimensional (3D)-cultured cells have attracted the attention of researchers in tissue engineering- and drug screening-related fields. Among them, 3D cellular fibers have attracted significant attention because they can be stacked to …
View article: Temporally controlled multistep division of DNA droplets for dynamic artificial cells
Temporally controlled multistep division of DNA droplets for dynamic artificial cells Open
Bio-soft matter droplets formed via liquid-liquid phase separation (LLPS) of biopolymers have been found in living cells. Synthetic LLPS droplets have recently been employed in nanobiotechnology for artificial cell construction, molecular …
View article: DNA-origami-armored DNA condensates
DNA-origami-armored DNA condensates Open
DNA condensates, formed by liquid-liquid phase separation (LLPS), emerge as promising soft matter assemblies for creating artificial cells. The advantages of DNA condensates are their molecular permeability through the surface due to their…
View article: Liquid DNA Coacervates form Porous Capsular Hydrogels via Viscoelastic Phase Separation on Microdroplet Interface
Liquid DNA Coacervates form Porous Capsular Hydrogels via Viscoelastic Phase Separation on Microdroplet Interface Open
Liquid–liquid phase separation (LLPS) droplets of biopolymers are known as functional microdroplets in living cells and have recently been used to construct protocells and artificial cells. The formation of DNA coacervates (also referred t…
View article: Pioneering artificial cell-like structures with DNA nanotechnology-based liquid-liquid phase separation
Pioneering artificial cell-like structures with DNA nanotechnology-based liquid-liquid phase separation Open
Recent studies have revealed that liquid-liquid phase separation (LLPS) plays crucial roles in various cellular functions. Droplets formed via LLPS within cells, often referred to as membraneless organelles, serve to concentrate specific m…
View article: Constructing virtual DNA-nanomachines
Constructing virtual DNA-nanomachines Open
View article: Fabrication and Cell Culture Applications of Core‐Shell Hydrogel Fibers Composed of Chitosan/DNA Interfacial Polyelectrolyte Complexation and Calcium Alginate: Straight and Beaded Core Variations (Adv. Healthcare Mater. 31/2023)
Fabrication and Cell Culture Applications of Core‐Shell Hydrogel Fibers Composed of Chitosan/DNA Interfacial Polyelectrolyte Complexation and Calcium Alginate: Straight and Beaded Core Variations (Adv. Healthcare Mater. 31/2023) Open
Core-Shell Hydrogel Fibers In article 2302011 by Kosuke Ino, Hitoshi Shiku, and co-workers, a simple and rapid method is presented for fabricating core-shell hydrogel fibers consisting of straight or beaded core fibers. Interfacial polyele…
View article: Programmable computational RNA droplets assembled via kissing-loop interaction
Programmable computational RNA droplets assembled via kissing-loop interaction Open
Programmable droplets, artificial liquid-like condensates of nucleic acids, are intelligent out-of-equilibrium systems that allow the critical aspects of their biological counterparts to be harnessed programmably, such as molecular sensing…
View article: Liquid DNA coacervates form porous capsular hydrogels via viscoelastic phase separation on microdroplet interface
Liquid DNA coacervates form porous capsular hydrogels via viscoelastic phase separation on microdroplet interface Open
Liquid-liquid phase separation (LLPS) droplets of biopolymers are known as functional microdroplets in living cells and have recently been used to construct protocells and artificial cells. The formation of DNA coacervates (also referred t…
View article: DNA droplets for intelligent and dynamical artificial cells: from the viewpoint of computation and non-equilibrium systems
DNA droplets for intelligent and dynamical artificial cells: from the viewpoint of computation and non-equilibrium systems Open
Living systems are molecular assemblies whose dynamics are maintained by non-equilibrium chemical reactions. To date, artificial cells have been studied from such physical and chemical viewpoints. This review briefly gives a perspective on…
View article: Fabrication and Cell Culture Applications of Core‐Shell Hydrogel Fibers Composed of Chitosan/DNA Interfacial Polyelectrolyte Complexation and Calcium Alginate: Straight and Beaded Core Variations
Fabrication and Cell Culture Applications of Core‐Shell Hydrogel Fibers Composed of Chitosan/DNA Interfacial Polyelectrolyte Complexation and Calcium Alginate: Straight and Beaded Core Variations Open
Core‐shell hydrogel fibers are widely used in cell culture applications. A simple and rapid method is presented for fabricating core‐shell hydrogel fibers, consisting of straight or beaded core fibers, for cell culture applications. The co…
View article: DNA Droplets: Intelligent, Dynamic Fluid (Adv. Biology 3/2023)
DNA Droplets: Intelligent, Dynamic Fluid (Adv. Biology 3/2023) Open
DNA Doplets In article 2200180, Masahiro Takinoue and co-workers show here that DNA droplets, a liquid-state micro-scale self-assembly of DNA sequences empower scientists and engineers to ‘program’ their sequence recognition capabilities a…
View article: Sequence-dependent fusion dynamics and physical properties of DNA droplets
Sequence-dependent fusion dynamics and physical properties of DNA droplets Open
Physical properties of DNA droplets formed by liquid–liquid phase separation of DNA nanostructures were investigated at a phase transition temperature. The surface tension was strongly influenced by the design of sticky ends.
View article: DNA Droplets: Intelligent, Dynamic Fluid
DNA Droplets: Intelligent, Dynamic Fluid Open
Breathtaking advances in DNA nanotechnology have established DNA as a promising biomaterial for the fabrication of programmable higher‐order nano/microstructures. In the context of developing artificial cells and tissues, DNA droplets have…