Masakazu Furuta
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View article: Surface Sterilization and X-ray Radiography of Woodblocks by Low-energy X-rays—Technology Transfer to Vietnam—
Surface Sterilization and X-ray Radiography of Woodblocks by Low-energy X-rays—Technology Transfer to Vietnam— Open
View article: Effect of gamma radiation on fungal growth stages and mechanical properties of traditional Japanese paper
Effect of gamma radiation on fungal growth stages and mechanical properties of traditional Japanese paper Open
View article: A theoretical model for evaluating a specific mode of lethal injury that causes reproductive death in an irradiated microbial population
A theoretical model for evaluating a specific mode of lethal injury that causes reproductive death in an irradiated microbial population Open
When microbial cells exposed to γ-rays and UV light are cultured in a liquid medium, reproductive death is observed. These cells can divide for one to several generations but cannot form visible colonies on an agar medium and are therefore…
View article: Development of a Sensitive Method for the Determination of 5,6-Dihydrothymidine Formed by Gamma Irradiation of DNA below 0.1 kGy
Development of a Sensitive Method for the Determination of 5,6-Dihydrothymidine Formed by Gamma Irradiation of DNA below 0.1 kGy Open
A sensitive method was developed to determine 5,6-dihydrothymidine (DHdThd) produced by relatively low-dose (<0.1 kGy) gamma irradiation of DNA. Previously, we had examined a method to determine the irradiation history of foods measured by…
View article: Detection of Gamma-Ray Irradiation History of Dried Plant-Based Foods <i>via</i> the Analysis of 5,6-Dihydrothymidine
Detection of Gamma-Ray Irradiation History of Dried Plant-Based Foods <i>via</i> the Analysis of 5,6-Dihydrothymidine Open
Irradiation is widely used worldwide to sterilize and kill insects in food, and prevent the germination of agricultural products. However, in Japan, food irradiation is prohibited except to prevent potato sprouting. Herein, 5,6-dihydrothym…
View article: Gamma-irradiated <i>Aspergillus</i> conidia show a growth curve with a reproductive death phase
Gamma-irradiated <i>Aspergillus</i> conidia show a growth curve with a reproductive death phase Open
In this study, we evaluated the effects of gamma irradiation on the germination of Aspergillus conidia and mycelial growth using microscopy and predictive microbiological modeling methods. A dose of 0.4 kGy reduced the germination rate by …
View article: Different patterns of germination inhibition by carvacrol and thymol in <i>Bacillus subtilis</i> spores
Different patterns of germination inhibition by carvacrol and thymol in <i>Bacillus subtilis</i> spores Open
This study aimed to clarify how the phenolic monoterpene carvacrol and its structural isomer thymol both as essential oil components (EOCs) inhibit the germination of Bacillus subtilis spore. Germination was evaluated by the OD600
View article: Preparation of High-quality Oil from Vegetable Oil with High-free Fatty Acid (FFA) Content Using an Ammonia/Methanol Solution
Preparation of High-quality Oil from Vegetable Oil with High-free Fatty Acid (FFA) Content Using an Ammonia/Methanol Solution Open
There are significant concerns regarding the quality of vegetable oils in the food and biofuel industries. In this study, we explored the preparation of high- quality oil from high-free fatty acid (FFA) vegetable oil using an ammonia/MeOH …
View article: Radiation Sensitivity of Aspergillus Niger of Low-Energy X-Rays and Ceasium-137 Gamma Rays
Radiation Sensitivity of Aspergillus Niger of Low-Energy X-Rays and Ceasium-137 Gamma Rays Open
View article: Excessive ultraviolet C irradiation causes spore protein denaturation and prohibits the initiation of spore germination in <i>Bacillus subtilis </i>
Excessive ultraviolet C irradiation causes spore protein denaturation and prohibits the initiation of spore germination in <i>Bacillus subtilis </i> Open
Ultraviolet (UV) -C is widely used to kill bacteria as it damages chromosomal DNA. We analyzed the denaturation of the protein function of Bacillus subtilis spores after UV-C irradiation. Almost all of the B. subtilis spores germinated in …
View article: Theory and application of growth delay analysis of colony formation for evaluation of injured population of the stressed fungal conidia
Theory and application of growth delay analysis of colony formation for evaluation of injured population of the stressed fungal conidia Open
A new concept of injured population assessment is proposed, in which the size of the injured population in stressed mold spores is evaluated by analyzing the colony formation process on a solid agar medium. In this method, a small paper di…
View article: (6) Wholesomeness of Irradiated Foods
(6) Wholesomeness of Irradiated Foods Open
放射線照射食品健全性についての考え方と欧米やわが国の長年に渡る健全性評価の先行研究について解説した。特に放射線照射により食品中の脂質から特異的に生成する2-アルキルシクロブタノン類(2-alkylcyclobutanones; 2-ACBs)の毒性試験に関して,最近のわが国で実施された研究成果を欧米の先行研究と対比して解説した。わが国の研究では,欧米の先行研究の試験条件を参考にし,より厳しい投与条件を用いて試験を行った。国際的なガイドラインに従って実施した遺伝毒性のバッテリ…
View article: Low Temperature Heating-Induced Death and Vacuole Injury in<i> Cladosporium sphaerospermum</i> Conidia
Low Temperature Heating-Induced Death and Vacuole Injury in<i> Cladosporium sphaerospermum</i> Conidia Open
The mechanism of thermal death of mold conidia has not been understood in detail. The purpose of this study is to analyze the death kinetics of heated conidia of Cladosporium sphaerospermum and to ascertain the expectant cell injury respon…
View article: Theoretical Base for the Application of the DiVSaL Method to Bacterial Spores to Evaluate Injured Populations Occurring After Exposure to Lethal Stress
Theoretical Base for the Application of the DiVSaL Method to Bacterial Spores to Evaluate Injured Populations Occurring After Exposure to Lethal Stress Open
To assess injury in bacterial spore populations exposed to lethal stress, we proposed a theoretical basis for applying the DiVSaL method, which has already been reported for general microorganisms as a double subculture method. We construc…
View article: Disinfection of Woodblocks of the Nguyen Dynasty of Vietnam by Low-Energy X-rays
Disinfection of Woodblocks of the Nguyen Dynasty of Vietnam by Low-Energy X-rays Open
The effects of low-energy X-ray irradiation were investigated as an intervention strategy for the disinfection of fungi-contaminated woodblocks. Fungi were isolated from the woodblocks of the Nguyen dynasty of Vietnam and Cladosporium sp. …
View article: Injured Microbes Wandering between Life and Death: Their Microbiology and Dynamic Behaviors
Injured Microbes Wandering between Life and Death: Their Microbiology and Dynamic Behaviors Open
2020年に発生した新型コロナウィルス感染症によるパンデミックは,人類に対する痛烈なカウンターパンチと言えよう.その一方では,抗生物質が効かない薬剤耐性菌の問題も次第に深刻化しつつある.われわれはこれら有害な感染体や微生物と共存しつつも,常に彼らと闘わなければならない.食品産業ではHACCP(危害分析重要管理点)が制度化され,食品を危害微生物から守るための安全体制が強化される.一方で,消費者ニーズに応えるべく,殺菌条件を緩和した高品質食品がトレンド化しつつある.これらの潮流…
View article: Detection Time Distribution of Microcolonies Formed by Individual Heat-Injured Cells of <i>Escherichia coli</i>
Detection Time Distribution of Microcolonies Formed by Individual Heat-Injured Cells of <i>Escherichia coli</i> Open
The microcolony formation at 30℃ on an enriched minimal salts agar plates by individual Escherichia coli cells heated at 50℃ was monitored with a time-lapse shadow image analysis system, MicroBio μ3DTM AutoScanner. While the tim…
View article: Application of gamma radiation for disinfection of fungi in a large volume of historical archives damaged by flood following Typhoon Hagibis 2019, Japan: A case report
Application of gamma radiation for disinfection of fungi in a large volume of historical archives damaged by flood following Typhoon Hagibis 2019, Japan: A case report Open
A large volume of historical archives was extensively damaged by flood water, following Typhoon Hagibis in Fukushima, Japan, in October 2019. They were rescued from the stricken area within a week, however, the prolonged exposure of paper …
View article: Biological effects of low-dose γ-ray irradiation on chromosomes and DNA of <i>Drosophila melanogaster</i>
Biological effects of low-dose γ-ray irradiation on chromosomes and DNA of <i>Drosophila melanogaster</i> Open
While the damage to chromosomes and genes induced by high-dose radiation (HDR) has been well researched in many organisms, the effects of low-dose radiation (LDR), defined as a radiation dose of ≤100 mSv, are still being debated. Recent re…
View article: A Modified Double Subculture Method for the Two-Mode Injuries Evaluation in a Stressed Fungal Spore Population
A Modified Double Subculture Method for the Two-Mode Injuries Evaluation in a Stressed Fungal Spore Population Open
In order to evaluate injury of a stressed fungal spore population, a modification of formerly presented double subculture method, which consists of both the conventional plate count method and the growth delay analysis method, was proposed…
View article: Estimation of Irradiation Doses of Raw Beef Liver Samples using 5,6-Dihydrothymidine as an Irradiation Marker
Estimation of Irradiation Doses of Raw Beef Liver Samples using 5,6-Dihydrothymidine as an Irradiation Marker Open
The quantities of (5S)- and (5R)-5,6-dihydrothymidine (total DHdThd) in frozen beef liver samples were compared between those irradiated by an electron beam (EB) and those subjected to γ-irradiation. DNA extracted from the sa…
View article: Thermotolerant Yeast <i>Kluyveromyces marxianus</i> Reveals More Tolerance to Heat Shock than the Brewery Yeast <i>Saccharomyces cerevisiae</i>
Thermotolerant Yeast <i>Kluyveromyces marxianus</i> Reveals More Tolerance to Heat Shock than the Brewery Yeast <i>Saccharomyces cerevisiae</i> Open
The thermotolerant yeast Kluyveromyces marxianus, growing at high temperature (45℃) , showed stronger survival under heat shock at 50℃ than the brewing yeast Saccharomyces cerevisiae, which was unable to grow at 45℃. The survival rate of K…
View article: Production of Biodiesel from Candlenut Oil Using a Two-step Co-solvent Method and Evaluation of Its Gaseous Emissions
Production of Biodiesel from Candlenut Oil Using a Two-step Co-solvent Method and Evaluation of Its Gaseous Emissions Open
Candlenut oil (CNO) is a potentially new feedstock for biodiesel (BDF) production. In this paper, a two-step co-solvent method for BDF production from CNO was examined. Firstly, esterification of free fatty acids (FFAs) (7 wt%) present in …
View article: Inhibitory Effect of Spice Powders on the Development of Heated and Irradiated <i>Bacillus subtilis</i> Spores as Evaluated by Calorimetry
Inhibitory Effect of Spice Powders on the Development of Heated and Irradiated <i>Bacillus subtilis</i> Spores as Evaluated by Calorimetry Open
Inhibitory effects of the powders of paprika, red pepper, black pepper, sage, oregano and thyme in a solid medium after heat treatment and gamma-irradiation on the development from spore of Bacillus subtilis were examined using calorimetry…
View article: A single-molecule assessment of the protective effect of DMSO against DNA double-strand breaks induced by photo-and γ-ray-irradiation, and freezing
A single-molecule assessment of the protective effect of DMSO against DNA double-strand breaks induced by photo-and γ-ray-irradiation, and freezing Open
Dimethyl sulfoxide (DMSO) is widely used as a cryoprotectant for organs, tissues, and cell suspension in storage. In addition, DMSO is known to be a useful free radical scavenger and a radio-protectant. To date, many in vitro assays using …
View article: Production of biodiesel from <i>Vernicia montana</i> Lour. oil using a co-solvent method and the subsequent evaluation of its stability during storage
Production of biodiesel from <i>Vernicia montana</i> Lour. oil using a co-solvent method and the subsequent evaluation of its stability during storage Open
The costs associated with the production of biodiesel fuel (BDF) are directly affected by the nature of the feedstock material and the methods used during the production process. In Southeast Asia, many non-edible oils are used to produce …
View article: Wetting-induced attraction of thin plates due to capillary flow
Wetting-induced attraction of thin plates due to capillary flow Open
Wetting-induced attraction are widely observed in microstructures where liquid flows along solid surfaces. Unexpected bending or collapse occurs if wetting-induced forces are neglected in the structural design, such as high aspect ratio pi…
View article: Stability of 2-alkylcyclobutanones in irradiated retort pouch <i>gyudon</i> topping during room temperature storage (2)
Stability of 2-alkylcyclobutanones in irradiated retort pouch <i>gyudon</i> topping during room temperature storage (2) Open
2-アルキルシクロブタノン（ACBs）は，放射線照射により食品中に含まれる脂肪酸から特異的に生じるため，食品の照射履歴の検知指標として有用な物質である。我々はこれまでに常温保存下でのACBsの安定性を評価することを目的として，レトルト包装された牛丼の具（レトルト牛丼）中に生成した2-ドデシルシクロブタノン（DCB）および2-テトラデシルシクロブタノン（TCB）の濃度を追跡し，照射後1年を経過してもACB濃度に大きな変化がないことを報告した。本研究では，さらに保存期間を3年に…
View article: Roles of Catalase and Trehalose in the Protection from Hydrogen Peroxide Toxicity in <i>Saccharomyces cerevisiae</i>
Roles of Catalase and Trehalose in the Protection from Hydrogen Peroxide Toxicity in <i>Saccharomyces cerevisiae</i> Open
The roles of catalase and trehalose in Saccharomyces cerevisiae subject to hydrogen peroxide (H2O2) treatment were examined by measuring the catalase activity and intracellular trehalose levels in mutants lacking catalase or trehalose synt…
View article: Isolation and Characterization of a Variant Manganese Resistant Strain of <i>Saccharomyces cerevisiae</i>
Isolation and Characterization of a Variant Manganese Resistant Strain of <i>Saccharomyces cerevisiae</i> Open
Manganese contamination in water is one of the most serious problems in Southeast Asian countries, including Vietnam. Bioremediation using microorganisms, especially from the brewing yeast Saccharomyces cerevisiae, is expected to be a usef…