Masanobu Kano
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View article: FMR1 mutant marmosets show fragile X syndrome phenotypes
FMR1 mutant marmosets show fragile X syndrome phenotypes Open
Fragile X syndrome (FXS) is the foremost monogenic cause of autism spectrum disorder and intellectual disability, caused by FMR1 gene silencing. Here, we report that common marmosets carrying FMR1 mutation, a non-human primate model for FX…
View article: Synaptic transmission is dispensable for selecting the winner input but is crucial for the subsequent events of synapse elimination
Synaptic transmission is dispensable for selecting the winner input but is crucial for the subsequent events of synapse elimination Open
Synaptic transmission has long been thought to regulate neuronal wiring during postnatal development, but this assumption remains largely untested. Selective strengthening of a single “winner” climbing fiber (CF) afferent to each Purkinje …
View article: Efficient Selection of DNA Aptamers with High Affinity and Specificity to GPCRs via Functional Extracellular Vesicles
Efficient Selection of DNA Aptamers with High Affinity and Specificity to GPCRs via Functional Extracellular Vesicles Open
Nucleic acid aptamers are promising new-generation experimental and therapeutic drugs for uncharacterized biomolecules and intractable diseases. However, efficient aptamer selection remains challenging. We present a novel functional select…
View article: The transcription factor ZFP64 promotes activity-dependent synapse elimination during postnatal cerebellar development
The transcription factor ZFP64 promotes activity-dependent synapse elimination during postnatal cerebellar development Open
Eliminating redundant synapses formed around birth is essential for shaping functionally mature neural circuits during postnatal development. Each Purkinje cell (PC) in the neonatal mouse cerebellum receives synaptic inputs from multiple c…
View article: A comprehensive suite for extracting neuron signals across multiple sessions in one-photon calcium imaging
A comprehensive suite for extracting neuron signals across multiple sessions in one-photon calcium imaging Open
We developed CaliAli, a comprehensive suite designed to extract neuronal signals from one-photon calcium imaging data collected across multiple sessions in free-moving conditions in mice. CaliAli incorporates information from blood vessels…
View article: Predictive reward-prediction errors of climbing fiber inputs integrate modular reinforcement learning with supervised learning
Predictive reward-prediction errors of climbing fiber inputs integrate modular reinforcement learning with supervised learning Open
Although the cerebellum is typically associated with supervised learning algorithms, it also exhibits extensive involvement in reward processing. In this study, we investigated the cerebellum’s role in executing reinforcement learning algo…
View article: Reduced GABAergic inhibition and impaired synapse elimination by neuroligin-2 deletion from Purkinje cells of the developing cerebellum
Reduced GABAergic inhibition and impaired synapse elimination by neuroligin-2 deletion from Purkinje cells of the developing cerebellum Open
Functionally mature neural circuits are shaped during postnatal development by eliminating redundant synapses formed around birth. This process is known as synapse elimination and requires a proper balance of excitation and inhibition. Neu…
View article: T-scope V4: miniaturized microscope for optogenetic tagging in freely behaving animals
T-scope V4: miniaturized microscope for optogenetic tagging in freely behaving animals Open
A miniaturized microscope (i.e., miniscope) enables the imaging of neuronal activity using calcium sensors while simultaneously manipulating that activity using opsins in freely moving animals. However, many miniscopes use light-emitting d…
View article: Direct and indirect pathways for heterosynaptic interaction underlying developmental synapse elimination in the mouse cerebellum
Direct and indirect pathways for heterosynaptic interaction underlying developmental synapse elimination in the mouse cerebellum Open
Developmental synapse elimination is crucial for shaping mature neural circuits. In the neonatal mouse cerebellum, Purkinje cells (PCs) receive excitatory synaptic inputs from multiple climbing fibers (CFs) and synapses from all but one CF…
View article: Delayed gramicidin delivery through an intra-pipette capillary facilitates perforated patch recordings
Delayed gramicidin delivery through an intra-pipette capillary facilitates perforated patch recordings Open
The gramicidin-perforated patch-clamp technique is indispensable for recording neuronal activities without changing the intracellular Cl- concentration. Conventionally, gramicidin contained in the pipette fluid is delivered to t…
View article: Molecular and cellular mechanisms of developmental synapse elimination in the cerebellum: Involvement of autism spectrum disorder-related genes
Molecular and cellular mechanisms of developmental synapse elimination in the cerebellum: Involvement of autism spectrum disorder-related genes Open
Neural circuits are initially created with excessive synapse formation until around birth and undergo massive reorganization until they mature. During postnatal development, necessary synapses strengthen and remain, whereas unnecessary one…
View article: Dynamic organization of cerebellar climbing fiber response and synchrony in multiple functional components reduces dimensions for reinforcement learning
Dynamic organization of cerebellar climbing fiber response and synchrony in multiple functional components reduces dimensions for reinforcement learning Open
Cerebellar climbing fibers convey diverse signals, but how they are organized in the compartmental structure of the cerebellar cortex during learning remains largely unclear. We analyzed a large amount of coordinate-localized two-photon im…
View article: Cerebellar climbing fibers multiplex movement and reward signals during a voluntary movement task in mice
Cerebellar climbing fibers multiplex movement and reward signals during a voluntary movement task in mice Open
Cerebellar climbing fibers convey sensorimotor information and their errors, which are used for motor control and learning. Furthermore, they represent reward-related information. Despite such functional diversity of climbing fiber signals…
View article: Author response: Dynamic organization of cerebellar climbing fiber response and synchrony in multiple functional components reduces dimensions for reinforcement learning
Author response: Dynamic organization of cerebellar climbing fiber response and synchrony in multiple functional components reduces dimensions for reinforcement learning Open
View article: PTPδ is a presynaptic organizer for the formation and maintenance of climbing fiber to Purkinje cell synapses in the developing cerebellum
PTPδ is a presynaptic organizer for the formation and maintenance of climbing fiber to Purkinje cell synapses in the developing cerebellum Open
Functionally mature neural circuits are shaped during postnatal development by eliminating redundant synapses formed during the perinatal period. In the cerebellum of neonatal rodents, each Purkinje cell (PC) receives synaptic inputs from …
View article: Distorted neurocomputation by a small number of extra-large spines in psychiatric disorders
Distorted neurocomputation by a small number of extra-large spines in psychiatric disorders Open
Human genetics strongly support the involvement of synaptopathy in psychiatric disorders. However, trans-scale causality linking synapse pathology to behavioral changes is lacking. To address this question, we examined the effects of synap…
View article: CaliAli, a tool for long-term tracking of neuronal population dynamics in calcium imaging
CaliAli, a tool for long-term tracking of neuronal population dynamics in calcium imaging Open
Neuron-tracking algorithms exhibit suboptimal performance in calcium imaging when the same neurons are not consistently detected, as unmatched features hinder intersession alignment. CaliAli addresses this issue by employing an alignment-b…
View article: Predictive reward-prediction errors of climbing fiber inputs integrate modular reinforcement learning with supervised learning
Predictive reward-prediction errors of climbing fiber inputs integrate modular reinforcement learning with supervised learning Open
Although the cerebellum is typically linked to supervised learning algorithms, it also exhibits extensive involvement in reward processing. In this study, we investigated the cerebellum’s role in executing reinforcement learning algorithms…
View article: Maturation of GABAergic Synaptic Transmission From Neocortical Parvalbumin Interneurons Involves N-methyl-D-aspartate Receptor Recruitment of Cav2.1 Channels
Maturation of GABAergic Synaptic Transmission From Neocortical Parvalbumin Interneurons Involves N-methyl-D-aspartate Receptor Recruitment of Cav2.1 Channels Open
N-methyl-D-aspartate receptor (NMDAR) hypofunction during brain development is likely to contribute to the manifestation of schizophrenia (SCZ) in young adulthood. The cellular targets of NMDAR hypofunction appear to be at least in part co…
View article: Dynamic organization of cerebellar climbing fiber response and synchrony in multiple functional modules reduces dimensions for reinforcement learning
Dynamic organization of cerebellar climbing fiber response and synchrony in multiple functional modules reduces dimensions for reinforcement learning Open
Daynamic functional organization by synchronization is theorized to be essential for dimension reduction of the cerebellar learning space. We analyzed a large amount of coordinate-localized, two-photon imaging data from cerebellar Crus II …
View article: Endocannabinoid-dependent formation of columnar axonal projection in the mouse cerebral cortex
Endocannabinoid-dependent formation of columnar axonal projection in the mouse cerebral cortex Open
Columnar structure is one of the most fundamental morphological features of the cerebral cortex and is thought to be the basis of information processing in higher animals. Yet, how such a topographically precise structure is formed is larg…
View article: Cerebellar climbing fibers convey behavioral information of multiplex modalities and form functional modules
Cerebellar climbing fibers convey behavioral information of multiplex modalities and form functional modules Open
Cerebellar climbing fibers (CFs) convey sensorimotor information and their errors, which are used for motor control and learning. Furthermore, they represent reward-related information. Despite such functional diversity of CF signals, it i…
View article: mGluR5 Is Substitutable for mGluR1 in Cerebellar Purkinje Cells for Motor Coordination, Developmental Synapse Elimination, and Motor Learning
mGluR5 Is Substitutable for mGluR1 in Cerebellar Purkinje Cells for Motor Coordination, Developmental Synapse Elimination, and Motor Learning Open
Group I metabotropic glutamate receptors (mGluRs) include mGluR1 and mGluR5, which are coupled to the Gq family of heterotrimeric G-proteins and readily activated by their selective agonist 3,5-dihydroxyphenilglycine (DHPG). mGluR1 and mGl…
View article: A Flp-dependent G-CaMP9a transgenic mouse for neuronal imaging in vivo
A Flp-dependent G-CaMP9a transgenic mouse for neuronal imaging in vivo Open
View article: Endocannabinoid-Mediated Control of Neural Circuit Excitability and Epileptic Seizures
Endocannabinoid-Mediated Control of Neural Circuit Excitability and Epileptic Seizures Open
Research on endocannabinoid signaling has greatly advanced our understanding of how the excitability of neural circuits is controlled in health and disease. In general, endocannabinoid signaling at excitatory synapses suppresses excitabili…
View article: Maturation of GABAergic Synaptic Transmission in Immature Neocortical Fast-Spiking Interneurons Involves NMDA Receptor Recruitment of Cav2.1 Channels
Maturation of GABAergic Synaptic Transmission in Immature Neocortical Fast-Spiking Interneurons Involves NMDA Receptor Recruitment of Cav2.1 Channels Open
View article: An Autism-Associated Neuroligin-3 Mutation Affects Developmental Synapse Elimination in the Cerebellum
An Autism-Associated Neuroligin-3 Mutation Affects Developmental Synapse Elimination in the Cerebellum Open
Neuroligin is a postsynaptic cell-adhesion molecule that is involved in synapse formation and maturation by interacting with presynaptic neurexin. Mutations in neuroligin genes, including the arginine to cystein substitution at the 451st a…
View article: Combined glyoxalase 1 dysfunction and vitamin B6 deficiency in a schizophrenia model system causes mitochondrial dysfunction in the prefrontal cortex
Combined glyoxalase 1 dysfunction and vitamin B6 deficiency in a schizophrenia model system causes mitochondrial dysfunction in the prefrontal cortex Open
View article: mGluR1 signaling in cerebellar Purkinje cells: Subcellular organization and involvement in cerebellar function and disease
mGluR1 signaling in cerebellar Purkinje cells: Subcellular organization and involvement in cerebellar function and disease Open
The cerebellum is essential for the control, coordination, and learning of movements, and for certain aspects of cognitive function. Purkinje cells are the sole output neurons in the cerebellar cortex and therefore play crucial roles in th…
View article: Combining electrophysiology and optogenetics for functional screening of pyramidal neurons in the mouse prefrontal cortex
Combining electrophysiology and optogenetics for functional screening of pyramidal neurons in the mouse prefrontal cortex Open