Masahiro Sato
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View article: Engineered Human Dental Pulp Stem Cells with Promising Potential for Regenerative Medicine
Engineered Human Dental Pulp Stem Cells with Promising Potential for Regenerative Medicine Open
The fields of regenerative medicine and stem cell-based tissue engineering hold great potential for treating a wide range of tissue and organ defects. Stem cells are ideal candidates for regenerative medicine because they are undifferentia…
View article: A Simple and Safe Protocol for Intra-Testicular Gene Delivery in Neonatal Mice Using a Convenient Isoflurane-Based Anesthesia System
A Simple and Safe Protocol for Intra-Testicular Gene Delivery in Neonatal Mice Using a Convenient Isoflurane-Based Anesthesia System Open
Newborn mice (up to 6 d after birth) are suitable for genetic manipulations, such as facial vein-mediated injection, owing to their hairless and thin skin. Their small body volumes also facilitate the rapid dissemination of injected soluti…
View article: Curative endoscopic submucosal dissection for esophageal squamous cell carcinoma after chemoradiotherapy for pharyngeal cancer: A case report
Curative endoscopic submucosal dissection for esophageal squamous cell carcinoma after chemoradiotherapy for pharyngeal cancer: A case report Open
BACKGROUND Esophageal squamous cell carcinoma (ESCC) is often managed with surgery, which is the first-line treatment option for stage I–III lesions. However, definitive chemoradiotherapy (dCRT) is associated with a recurrence rate of 30% …
View article: Pitiríase Versicolor: Aspectos clínicos, diagnósticos e avanços no tratamento da infecção por Malassezia spp.
Pitiríase Versicolor: Aspectos clínicos, diagnósticos e avanços no tratamento da infecção por Malassezia spp. Open
A pitiríase versicolor é uma infecção fúngica superficial causada por leveduras do gênero Malassezia, parte da microbiota normal da pele, mas que pode se tornar patogênica em condições específicas. Caracteriza-se por máculas ou placas de c…
View article: Elucidation of Coloring Molecule Using a Simple Identification Method with a Color Reaction for <i>Omphalotus japonicus</i>
Elucidation of Coloring Molecule Using a Simple Identification Method with a Color Reaction for <i>Omphalotus japonicus</i> Open
For Omphalotus japonicus, the coloring molecule was found and characterized using a simple method of identification with a color reaction. The compound that chang color under basic conditions was isolated from a methanolic extract of O. ja…
View article: Possible Application of GONAD/<i>i</i>-GONAD, a Novel <i>In Vivo</i> Genome Editing Technology, to Generate Genome-edited Large Experimental Animals
Possible Application of GONAD/<i>i</i>-GONAD, a Novel <i>In Vivo</i> Genome Editing Technology, to Generate Genome-edited Large Experimental Animals Open
View article: Efficient genome editing of two-cell mouse embryos via modified CRISPR/Cas electroporation
Efficient genome editing of two-cell mouse embryos via modified CRISPR/Cas electroporation Open
Creating genetically modified (GM) animals using CRISPR/Cas mediated through the electroporation of two-cell stage embryos, rather than fertilized eggs, holds considerable potential. The full potential of genome editing using two-cell stag…
View article: Scratch-Based Isolation of Primary Cells (SCIP): A Novel Method to Obtain a Large Number of Human Dental Pulp Cells Through One-Step Cultivation
Scratch-Based Isolation of Primary Cells (SCIP): A Novel Method to Obtain a Large Number of Human Dental Pulp Cells Through One-Step Cultivation Open
Background: Dental pulp (DP) is a connective tissue composed of various cell types, including fibroblasts, neurons, adipocytes, endothelial cells, and odontoblasts. It contains a rich supply of pluripotent stem cells, making it an importan…
View article: In vivo functional significance of direct physical interaction between Period and Cryptochrome in mammalian circadian rhythm generation
In vivo functional significance of direct physical interaction between Period and Cryptochrome in mammalian circadian rhythm generation Open
In the current model, the auto-negative feedback action of Period (Per) and Cryptochrome (Cry) on their own transcription is the hallmark mechanism driving cell-autonomous circadian rhythms. Although this model likely makes sense even if P…
View article: Artificial Insemination as a Possible Convenient Tool to Acquire Genome-Edited Mice via In Vivo Fertilization with Engineered Sperm
Artificial Insemination as a Possible Convenient Tool to Acquire Genome-Edited Mice via In Vivo Fertilization with Engineered Sperm Open
Advances in genome editing technology have made it possible to create genome-edited (GE) animals, which are useful for identifying isolated genes and producing models of human diseases within a short period of time. The production of GE an…
View article: Loss of Cell-Cell Contact Inhibits Cellular Differentiation of α-Catenin Knock Out P19 Embryonal Carcinoma Cells and Their Colonization into the Developing Mouse Embryos
Loss of Cell-Cell Contact Inhibits Cellular Differentiation of α-Catenin Knock Out P19 Embryonal Carcinoma Cells and Their Colonization into the Developing Mouse Embryos Open
Cadherin−catenin cell−cell adhesion complexes, composed of cadherin, β-catenin or plakoglobin, and α-catenin (α-cat) molecules, are crucial for maintaining cell−cell contact and are commonly referred to as “adherens junctions (AJs).” Inact…
View article: Machine learning-based reproducible prediction of type 2 diabetes subtypes
Machine learning-based reproducible prediction of type 2 diabetes subtypes Open
View article: Gene-gene functional relationships in Alzheimer's disease: CELF1 regulates KLC1 alternative splicing
Gene-gene functional relationships in Alzheimer's disease: CELF1 regulates KLC1 alternative splicing Open
The causes of Alzheimer's disease (AD) are poorly understood, although many genes are known to be involved in this pathology. To gain insights into the underlying molecular mechanisms, it is essential to identify the relationships between …
View article: Recent Advances in the Production of Genome-Edited Animals Using <i>i</i>-GONAD, a Novel <i>in vivo</i> Genome Editing System, and Its Possible Use for the Study of Female Reproductive Systems
Recent Advances in the Production of Genome-Edited Animals Using <i>i</i>-GONAD, a Novel <i>in vivo</i> Genome Editing System, and Its Possible Use for the Study of Female Reproductive Systems Open
Gene-engineered animals created using gene-targeting technology have long been recognized as beneficial, valid, and valuable tools for exploring the function of a gene of interest, at least in early 2013. This approach, however, suffers fr…
View article: Recent Advances in In Vivo Somatic Cell Gene Modification in Newborn Pups
Recent Advances in In Vivo Somatic Cell Gene Modification in Newborn Pups Open
Germline manipulation at the zygote stage using the CRISPR/Cas9 system has been extensively employed for creating genetically modified animals and maintaining established lines. However, this approach requires a long and laborious task. Re…
View article: The clinical efficacy and safety of granulocyte and monocyte adsorptive apheresis in patients with Crohn’s disease: A multicenter retrospective cohort study
The clinical efficacy and safety of granulocyte and monocyte adsorptive apheresis in patients with Crohn’s disease: A multicenter retrospective cohort study Open
Background: A remission induction therapy of granulocyte and monocyte adsorptive apheresis (GMA) with Adacolumn was given to patients with active Crohn’s disease (CD). However, establishing an appropriate treatment strategy for GMA in pati…
View article: Recent Genome-Editing Approaches toward Post-Implanted Fetuses in Mice
Recent Genome-Editing Approaches toward Post-Implanted Fetuses in Mice Open
Genome editing, as exemplified by the CRISPR/Cas9 system, has recently been employed to effectively generate genetically modified animals and cells for the purpose of gene function analysis and disease model creation. There are at least fo…
View article: Relationships among Postprandial Plasma Active GLP-1 and GIP Excursions, Skeletal Muscle Mass, and Body Fat Mass in Patients with Type 2 Diabetes Treated with Either Miglitol, Sitagliptin, or Their Combination: A Secondary Analysis of the MASTER Study
Relationships among Postprandial Plasma Active GLP-1 and GIP Excursions, Skeletal Muscle Mass, and Body Fat Mass in Patients with Type 2 Diabetes Treated with Either Miglitol, Sitagliptin, or Their Combination: A Secondary Analysis of the MASTER Study Open
Background: We previously conducted a pilot randomized controlled trial “the MASTER study” and demonstrated that alpha-glucosidase inhibitor miglitol and a dipeptidyl peptidase-4 inhibitor sitagliptin modified postprandial plasma excursion…
View article: A nutritional assessment tool, GNRI, predicts sarcopenia and its components in type 2 diabetes mellitus: A Japanese cross-sectional study
A nutritional assessment tool, GNRI, predicts sarcopenia and its components in type 2 diabetes mellitus: A Japanese cross-sectional study Open
Background There are few reports evaluating the relationship between undernutrition and the risk of sarcopenia in type 2 diabetes mellitus (T2DM) patients. Objective We investigated whether undernutritional status assessed by the geriatric…
View article: The Role of Genetically Modified Human Feeder Cells in Maintaining the Integrity of Primary Cultured Human Deciduous Dental Pulp Cells
The Role of Genetically Modified Human Feeder Cells in Maintaining the Integrity of Primary Cultured Human Deciduous Dental Pulp Cells Open
Tissue-specific stem cells exist in tissues and organs, such as skin and bone marrow. However, their pluripotency is limited compared to embryonic stem cells. Culturing primary cells on plastic tissue culture dishes can result in the loss …
View article: Gender-Difference in Hair Length as Revealed by Crispr-Based Production of Long-Haired Mice with Dysfunctional FGF5 Mutations
Gender-Difference in Hair Length as Revealed by Crispr-Based Production of Long-Haired Mice with Dysfunctional FGF5 Mutations Open
Fibroblast growth factor 5 (FGF5) is an important molecule required for the transition from anagen to catagen phase of the mammalian hair cycle. We previously reported that Syrian hamsters harboring a 1-bp deletion in the Fgf5 gene exhibit…
View article: Cytoplasmic Microinjection of <i>piggyBac</i> Transposase mRNA and Transposon Vectors for Efficient <i>In Vitro</i> Production of Transgenic Porcine Parthenotes
Cytoplasmic Microinjection of <i>piggyBac</i> Transposase mRNA and Transposon Vectors for Efficient <i>In Vitro</i> Production of Transgenic Porcine Parthenotes Open
The efficient production of transgenic (Tg) piglets has remained a challenge in the field of domestic animal studies. Unlike mice, the pronuclei of pig zygotes cannot be easily studied because of the abundance of lipid droplets. Therefore,…
View article: Recent Advances in <i>In Vivo</i> Genome Editing Targeting Mammalian Preimplantation Embryos
Recent Advances in <i>In Vivo</i> Genome Editing Targeting Mammalian Preimplantation Embryos Open
CRISPR-based genome engineering has been widely used for producing gene-modified animals such as mice and rats, to explore the function of a gene of interest and to create disease models. However, it always requires the ex vivo handling of…
View article: Successful i-GONAD in Mice at Early Zygote Stage through In Vivo Electroporation Three Min after Intraoviductal Instillation of CRISPR-Ribonucleoprotein
Successful i-GONAD in Mice at Early Zygote Stage through In Vivo Electroporation Three Min after Intraoviductal Instillation of CRISPR-Ribonucleoprotein Open
Improved genome editing via oviductal nucleic acids delivery (i-GONAD) is a new technology enabling in situ genome editing of mammalian zygotes exiting the oviductal lumen, which is now available in mice, rats, and hamsters. In this method…
View article: CRISPR Technology - Recent Advances
CRISPR Technology - Recent Advances Open
CRISPR technology has been extensively used in vitro and in vivo as a tool in basic research for genetic editing (e.g., genome encoding, silencing, enhancing, and modification). Although there are many technical and ethical challenges to o…
View article: Direct Injection of Recombinant AAV-Containing Solution into the Oviductal Lumen of Pregnant Mice Caused In Situ Infection of Both Preimplantation Embryos and Oviductal Epithelium
Direct Injection of Recombinant AAV-Containing Solution into the Oviductal Lumen of Pregnant Mice Caused In Situ Infection of Both Preimplantation Embryos and Oviductal Epithelium Open
Adeno-associated virus (AAV) vector is an efficient viral-based gene delivery tool used with many types of cells and tissues, including neuronal cells and muscles. AAV serotype 6 (AAV-6), one of numerous AAV serotypes, was recently found t…
View article: Electroporation-Based Non-Viral Gene Delivery to Adipose Tissue in Mice
Electroporation-Based Non-Viral Gene Delivery to Adipose Tissue in Mice Open
Adipose tissue is distributed throughout the body as fat depots. The amount of adipose tissue increases with age. In mice, epididymal fat depots in males and gonadal fat depots in females are associated with the reproductive system. Regard…
View article: An Alzheimer’s disease pathway uncovered by functional omics: the risk gene <i>CELF1</i> regulates <i>KLC1</i> splice variant E expression, which drives Aβ pathology
An Alzheimer’s disease pathway uncovered by functional omics: the risk gene <i>CELF1</i> regulates <i>KLC1</i> splice variant E expression, which drives Aβ pathology Open
In an era when numerous disease-associated genes have been identified, determining the molecular mechanisms of complex diseases is still difficult. The CELF1 region was identified by genome-wide association studies as an Alzheimer’s diseas…
View article: Recent Advances in the Production of Genome-Edited Rats
Recent Advances in the Production of Genome-Edited Rats Open
The rat is an important animal model for understanding gene function and developing human disease models. Knocking out a gene function in rats was difficult until recently, when a series of genome editing (GE) technologies, including zinc-…