Masayo Kushiro
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View article: Perioperative Arrhythmia of Unknown Etiology in a Cat: Infection Involvement?
Perioperative Arrhythmia of Unknown Etiology in a Cat: Infection Involvement? Open
Perioperative arrhythmias may be life-threatening if left untreated, and diverse etiologies should be considered. We encountered an arrhythmia of unknown etiology in a cat with a chronic urinary tract infection (UTI). A domestic short-hair…
View article: A dataset of the distribution of aflatoxin-producing fungi in Japan
A dataset of the distribution of aflatoxin-producing fungi in Japan Open
View article: NARO WAGRI-API (Data & Program)
NARO WAGRI-API (Data & Program) Open
View article: Trial for <i>cyp51A</i> gene variants to discriminate Japanese <i>Aspergillus oryzae</i> from <i>A. flavus</i>
Trial for <i>cyp51A</i> gene variants to discriminate Japanese <i>Aspergillus oryzae</i> from <i>A. flavus</i> Open
View article: Short-Term Oral Administration of 1.5 μg/kg bw/day of Deoxynivalenol Significantly Exacerbates Inflammatory and Itching Symptoms in a Mouse Model of Imiquimod-Induced Psoriasis
Short-Term Oral Administration of 1.5 μg/kg bw/day of Deoxynivalenol Significantly Exacerbates Inflammatory and Itching Symptoms in a Mouse Model of Imiquimod-Induced Psoriasis Open
Deoxynivalenol (DON) is a mycotoxin commonly found worldwide and is implicated in various health effects. We recently demonstrated that subacute oral exposure to DON significantly exacerbates symptoms of type 2 helper T-cell-mediated aller…
View article: Targeting Mitochondria to Inhibit Aflatoxin Production: Mechanistic Insight
Targeting Mitochondria to Inhibit Aflatoxin Production: Mechanistic Insight Open
Contamination of agricultural crops by aflatoxin, a potent carcinogenic fungal toxin, is a global issue that poses serious health risks to humans and livestock while inflicting significant economic damage on the agricultural sector. Specif…
View article: YES-DC-CP medium facilitates the isolation of <i>Aspergillus</i> section <i>Flavi</i> from acidic field soils
YES-DC-CP medium facilitates the isolation of <i>Aspergillus</i> section <i>Flavi</i> from acidic field soils Open
View article: Comparative Genome Analysis of Japanese Field-Isolated Aspergillus for Aflatoxin Productivity and Non-Productivity
Comparative Genome Analysis of Japanese Field-Isolated Aspergillus for Aflatoxin Productivity and Non-Productivity Open
Aspergillus flavus produces aflatoxin, a carcinogenic fungal toxin that poses a threat to the agricultural and food industries. There is a concern that the distribution of aflatoxin-producing A. flavus is expanding in Japan due to climate …
View article: Occurrence Evaluation of Aflatoxigenic <i>Aspergilli</i> in Thai Corn Using Dichlorvos-ammonia and Whole-agar Extraction Methods
Occurrence Evaluation of Aflatoxigenic <i>Aspergilli</i> in Thai Corn Using Dichlorvos-ammonia and Whole-agar Extraction Methods Open
Aflatoxins and aflatoxigenic fungi are hazardous to food security and safety since mycotoxins and related fungi in cereals significantly affect animal and human health. The relatively high frequency of aflatoxigenic Aspergilli isolates in …
View article: Low-dose ethanol increases aflatoxin production due to the adh1-dependent incorporation of ethanol into aflatoxin biosynthesis
Low-dose ethanol increases aflatoxin production due to the adh1-dependent incorporation of ethanol into aflatoxin biosynthesis Open
View article: Whole agar dish culture extraction method to assess the survival of aflatoxigenic fungi in soil samples
Whole agar dish culture extraction method to assess the survival of aflatoxigenic fungi in soil samples Open
In this study, we investigated a method of extracting the whole agar dish culture to assess the existence and survival of producing fungi of aflatoxins (AFs) in field soil samples. In a control experiment using a known aflatoxigenic Asperg…
View article: Effects of Four Isothiocyanates in Dissolved and Gaseous States on the Growth and Aflatoxin Production of Aspergillus flavus In Vitro
Effects of Four Isothiocyanates in Dissolved and Gaseous States on the Growth and Aflatoxin Production of Aspergillus flavus In Vitro Open
Aflatoxins (AFs), a class of toxins produced by certain species of the genus Aspergillus, occasionally contaminate food and cause serious damage to human health and the economy. AFs contamination is a global problem, and there is a need to…
View article: Preparation of Monoclonal Antibodies Specifically Reacting with the Trichothecene Mycotoxins Nivalenol and 15-Acetylnivalenol via the Introduction of a Linker Molecule into Its C-15 Position
Preparation of Monoclonal Antibodies Specifically Reacting with the Trichothecene Mycotoxins Nivalenol and 15-Acetylnivalenol via the Introduction of a Linker Molecule into Its C-15 Position Open
Nivalenol (NIV) is a trichothecene mycotoxin that is more toxic than deoxynivalenol. It accumulates in grains due to infection with Fusarium species, which are the causative agents of scab or Fusarium head blight. An immunoassay, which is …
View article: A Novel Medium for Isolating Two Japanese Species in the Fusarium graminearum Species Complex and a Dipstick DNA Chromatography Assay for Species Identification and Trichothecene Typing
A Novel Medium for Isolating Two Japanese Species in the Fusarium graminearum Species Complex and a Dipstick DNA Chromatography Assay for Species Identification and Trichothecene Typing Open
Members of the Fusarium graminearum species complex (Fg complex) are the primary pathogens that cause Fusarium head blight in wheat and barley. Fg complex members grow poorly on Fusarium oxysporum-selective media, such as Komada and Fo-G2,…
View article: Extraction efficiency of kojic acid from ammonia-treated or non-treated agar medium with various methanol aqueous solvents
Extraction efficiency of kojic acid from ammonia-treated or non-treated agar medium with various methanol aqueous solvents Open
Kojic acid is one of the major secondary metabolites produced by fungal species of Aspergillus section Flavi. In this study, we compared the extraction efficiency of kojic acid from agar media using the five solvents of different mixing ra…
View article: Low-Dose Ethanol Increases Aflatoxin Production in <i>Aspergillus Flavus</i> Due to the <i>Adh1</i>-Dependent Incorporation of Ethanol Into Aflatoxin Biosynthesis
Low-Dose Ethanol Increases Aflatoxin Production in <i>Aspergillus Flavus</i> Due to the <i>Adh1</i>-Dependent Incorporation of Ethanol Into Aflatoxin Biosynthesis Open
View article: Rapid and Reliable Analysis of Kojic Acid in Fungal Agar
Rapid and Reliable Analysis of Kojic Acid in Fungal Agar Open
A rapid HPLC-UV method was developed for the quantitative analysis of kojic acid contained in fungal agar media. The methanol extract from fungal agar media was diluted with acetic acid aqueous solution and injected into HPLC. The separati…
View article: Isolation of <i>Aspergillus flavus</i> strains from field soil by the improved DV-AM method
Isolation of <i>Aspergillus flavus</i> strains from field soil by the improved DV-AM method Open
本研究では,つくばの実験圃場中の菌を,改良ジクロルボス-アンモニア(DV-AM)法を用いてスクリーニングし,手法の適用性を検証することを目的とする.DV-AM法は,目視の赤(陽性)白(陰性)判定によるアフラトキシン(AF)産生菌コロニーの検出法であり,培地組成の改良により高感度の検出が可能となっている.7月(立毛の植物体が有る時期)に実験圃場土壌を採取し,本法によるスクリーニングを行った結果,20地点から採取した土壌のうち3点から計4株の陽性株が分離された.この4株について…
View article: Detection of <i>Aspergillus novoparasiticus</i> from Japanese sugarcane field by the dichlorvos-ammonia (DV-AM) method with single colony AM assay
Detection of <i>Aspergillus novoparasiticus</i> from Japanese sugarcane field by the dichlorvos-ammonia (DV-AM) method with single colony AM assay Open
The dichlorvos-ammonia (DV-AM) method followed by single colony AM assay was applied for re-screening of aflatoxigenic fungi from soil samples from sugarcane field in Okinawa, Japan. In previous study, we obtained two aflatoxigenic isolate…
View article: Detection of aflatoxigenic fungi in imported raw nuts using the dichlorvos-ammonia (DV-AM) method
Detection of aflatoxigenic fungi in imported raw nuts using the dichlorvos-ammonia (DV-AM) method Open
The dichlorvos-ammonia (DV-AM) method is a simple and sensitive visual method for detecting aflatoxigenic fungi. Since aflatoxin contamination has been frequently reported in ground nuts and tree nuts in many countries, we herein used the …
View article: Comparative effects of allyl and methyl isothiocyanates on aflatoxin production and growth of <i>Aspergillus flavus</i>
Comparative effects of allyl and methyl isothiocyanates on aflatoxin production and growth of <i>Aspergillus flavus</i> Open
The study to evaluate the effects of isothiocyanate compounds (ITCs) on the production of aflatoxin B1 (AFB1) by an aflatoxigenic fungus was conducted. Allyl isothiocyanate (AITC) and methyl isothiocyanate (MITC) were tested for the inhibi…
View article: Occurrence of aflatoxins in processed chili pepper sold in Myanmar
Occurrence of aflatoxins in processed chili pepper sold in Myanmar Open
ミャンマーの市場で2017年に市販されていた加工唐辛子(CP)10点について,アフラトキシン類の汚染調査を行った.すべての市販品は乾燥して粉末にされたものであった.主要アフラトキシンであるアフラトキシンB1(AFB1),アフラトキシンB2(AFB2),アフラトキシンG1(AFG1),およびアフラトキシンG2(AFG2)について,イムノアフィニティカラム精製後にHPLC-蛍光法で定量分析を行った.各分析値は,添加回収試験により確認した.1試料(CP-3)を除く全試料からAFB…
View article: Improvement of the Culture Medium for the Dichlorvos-Ammonia (DV-AM) Method to Selectively Detect Aflatoxigenic Fungi in Soil
Improvement of the Culture Medium for the Dichlorvos-Ammonia (DV-AM) Method to Selectively Detect Aflatoxigenic Fungi in Soil Open
The dichlorvos-ammonia (DV-AM) method is a simple but sensitive visual method for detecting aflatoxigenic fungi. Here we sought to develop a selective medium that is appropriate for the growth of aflatoxigenic fungi among soil mycoflora. W…
View article: Corrigendum
Corrigendum Open
View article: Visit to Aflasafe factory in Kenya Agricultural & Livestock Research Organization (KALRO) Katumani Research Centre, Machakos, Kenya
Visit to Aflasafe factory in Kenya Agricultural & Livestock Research Organization (KALRO) Katumani Research Centre, Machakos, Kenya Open
昨年２月に，Annette Nyangaresi氏の手配とMutegi Charity博士の好意により，ケニア共和国マチャコス県に在るケニア農業・畜産研究機構（KALRO）カツマニ研究センターに付随したアフラセーフ製造工場を見学させていただきました．Annette Nyangaresi氏，彼女の指導教官の藤井義晴先生（東京農工大），Willis Owino教授（ジョモケニアッタ農工大学）とご一緒でした．その概要を紹介します．
View article: Detection of Aflatoxigenic and Atoxigenic Mexican Aspergillus Strains by the Dichlorvos–Ammonia (DV–AM) Method
Detection of Aflatoxigenic and Atoxigenic Mexican Aspergillus Strains by the Dichlorvos–Ammonia (DV–AM) Method Open
The dichlorvos–ammonia (DV–AM) method is a sensitive method for distinguishing aflatoxigenic fungi by detecting red (positive) colonies. In this study, the DV–AM method was applied for the isolation of aflatoxigenic and atoxigenic fungi fr…
View article: Isolation of minor aflatoxigenic fungi using dichlorvos-ammonia (DV-AM) method
Isolation of minor aflatoxigenic fungi using dichlorvos-ammonia (DV-AM) method Open
ジクロルボス-アンモニア(DV-AM)法は,目視の赤(陽性)白(陰性)判定によるアフラトキシン産生菌コロニーの高感度検出法である.本研究では,DV-AM法を用いて,沖縄のさとうきび圃場からアフラトキシン産生菌の分離を試みた.初回のスクリーニングで土壌25サンプル中1サンプルから6株の陽性株が分離できた.それらは化学分析により,BタイプだけでなくGタイプのアフラトキシンを産生することが確認できた.6株中2株(OKI-12,OKI-16)について形態学的ならびに分子系統学的解析…
View article: Analysis of the Masked Metabolite of Deoxynivalenol and Fusarium Resistance in CIMMYT Wheat Germplasm
Analysis of the Masked Metabolite of Deoxynivalenol and Fusarium Resistance in CIMMYT Wheat Germplasm Open
Fusarium head blight (FHB) causes significant grain loss and contamination of grains with harmful mycotoxins, especially deoxynivalenol (DON). Fusarium resistance and DON accumulation have been extensively investigated in various cultivars…
View article: Highlights from ISMYCO 2016 and the Asian Network Meeting
Highlights from ISMYCO 2016 and the Asian Network Meeting Open
The Asian Network Meeting was held in Tokyo, Japan on 2 Dec. 2016, as part of ISMYCO 2016 (International Symposium of Mycotoxicology). This meeting was the first to take place in Japan since the 2003 meeting in Kagawa. This short manuscrip…
View article: Improvement of mobile phase in thin-layer chromatography for aflatoxins and analysis of the effect of dichlorvos in aflatoxigenic fungi
Improvement of mobile phase in thin-layer chromatography for aflatoxins and analysis of the effect of dichlorvos in aflatoxigenic fungi Open
Thin-layer chromatography for aflatoxins using toluene as a constituent of a mobile phase worked well and was able to avoid the usage of chloroform. We analyzed the effect of dichlorvos in aflatoxigenic fungi using above mobile phase, and …