Masayuki Ishihara
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View article: Suppression and Prediction of Crack Tip Stress Concentration in Thermoelectric Elastic Materials
Suppression and Prediction of Crack Tip Stress Concentration in Thermoelectric Elastic Materials Open
View article: Recent Progress on Heparin–Protamine Particles for Biomedical Application
Recent Progress on Heparin–Protamine Particles for Biomedical Application Open
Biomolecules are attractive building blocks with self-assembly ability, structural diversity, and excellent functionality for creating artificial materials. Heparin and protamine, a clinically relevant pair of biomolecules used in cardiac …
View article: Effectivity of Scallop Shell-Derived Calcium Oxide Water in Comparison with Hypochlorous Acid and Ethanol as General-Purpose Disinfectants for Environmental Surfaces
Effectivity of Scallop Shell-Derived Calcium Oxide Water in Comparison with Hypochlorous Acid and Ethanol as General-Purpose Disinfectants for Environmental Surfaces Open
貝殻焼成酸化カルシウム水（BiSCaO Water）は，ホタテ貝殻由来酸化カルシウムを基にした高濃度カルシウムイオン溶液であり，pH ≥12.7の無色透明な液体である．BiSCaO Waterは広範囲の殺微生物・ウイルス不活化効果を有しており，さらに生体や環境に優しい物質であることが知られている．本研究は，環境表面のための洗浄・除菌剤としてのBiSCaO Waterの有効性を，エタノールや次亜塩素酸水（200 ppm，pH 6.2）と比較して評価することを目的とした．アデノ…
View article: Langerhans cell histiocytosis in the frontal and zygomatic bones of an adolescent girl
Langerhans cell histiocytosis in the frontal and zygomatic bones of an adolescent girl Open
An adolescent girl with no medical history presented to our institution with right upper eyelid swelling and pain. She initially went to an external ophthalmological clinic, where she was given eye medications due to the suspicion of ocula…
View article: Severe lateral sinus thrombosis during the postpartum period that required decompressive craniectomy
Severe lateral sinus thrombosis during the postpartum period that required decompressive craniectomy Open
A 34-year-old woman presented to our unit with severe headache and nausea 18 days after a caesarean section. Her medical history included an epidural anaesthesia for vaginal delivery and a recent general anaesthesia for caesarean section, …
View article: Recent Progress in the Development of Disinfectants from Scallop Shell-Derived Calcium Oxide for Clinical and Daily Use
Recent Progress in the Development of Disinfectants from Scallop Shell-Derived Calcium Oxide for Clinical and Daily Use Open
The current pandemic of novel coronavirus disease (COVID-19) has highlighted the importance of disinfectants. As a raw material for next-generation disinfectants, scallop shell-derived calcium oxide (CaO) has been revealed to exhibit signi…
View article: Efficacy of Bioshell Calcium Oxide Water as Disinfectants to Enable Face Mask Reuse
Efficacy of Bioshell Calcium Oxide Water as Disinfectants to Enable Face Mask Reuse Open
Bioshell calcium oxide (BiSCaO) is derived from scallop shells and after heat treatment exhibits broad microbicidal activity. BiSCaO Water is a disinfectant prepared by collecting the aqueous layer after adding BiSCaO powder to water, is c…
View article: In vivo Hepatocyte Genome Manipulation via Intravenous Injection of Genome Editing Components
In vivo Hepatocyte Genome Manipulation via Intravenous Injection of Genome Editing Components Open
The liver is a major organ with a wide range of functions, including detoxification, protein synthesis, and bile production. Liver dysfunction causes liver diseases such as hepatic cirrhosis and hepatitis. To explore the pathogenesis of th…
View article: Safety of Concentrated Bioshell Calcium Oxide Water Application for Surface and Skin Disinfections against Pathogenic Microbes
Safety of Concentrated Bioshell Calcium Oxide Water Application for Surface and Skin Disinfections against Pathogenic Microbes Open
Immediately post-production, commercially available bioshell calcium oxide (BiSCaO) water is colorless, transparent, and strongly alkaline (pH 12.8), and is known to possess deodorizing properties and broad microbicidal activity. However, …
View article: Development of Novel Heparin/Protamine Nanoparticles Useful for Delivery of Exogenous Proteins In Vitro and In Vivo
Development of Novel Heparin/Protamine Nanoparticles Useful for Delivery of Exogenous Proteins In Vitro and In Vivo Open
We previously reported that heparin/protamine particles (LHPPs) produced as nanoparticles through simple mixing of raw materials exhibit sustained protein release and can be retained in cells. In the present study, we modified LHPPs withou…
View article: Hydrodynamics-Based Transplacental Delivery as a Useful Noninvasive Tool for Manipulating Fetal Genome
Hydrodynamics-Based Transplacental Delivery as a Useful Noninvasive Tool for Manipulating Fetal Genome Open
We previously demonstrated that the injection of pregnant wild-type female mice (carrying enhanced green fluorescent protein (EGFP)-expressing transgenic fetuses) at embryonic day (E) 12.5 with an all-in-one plasmid conferring the expressi…
View article: Bioshell Calcium Oxide-Containing Liquids as a Sanitizer for the Reduction of Histamine Production in Raw Japanese Pilchard, Japanese Horse Mackerel, and Chub Mackerel
Bioshell Calcium Oxide-Containing Liquids as a Sanitizer for the Reduction of Histamine Production in Raw Japanese Pilchard, Japanese Horse Mackerel, and Chub Mackerel Open
Recently, there has been an increase in the number of food poisoning cases associated with histamine in food, mainly in relation to histamine in fish. Here, we investigated methods to decrease histamine levels in Japanese pilchard, Japanes…
View article: Concentrated Bioshell Calcium Oxide (BiSCaO) Water Kills Pathogenic Microbes: Characterization and Activity
Concentrated Bioshell Calcium Oxide (BiSCaO) Water Kills Pathogenic Microbes: Characterization and Activity Open
Bioshell calcium oxide (BiSCaO) exhibits deodorizing properties and broad microbicidal activity. In this study, we examined possible utility of BiSCaO Water for that purpose. BiSCaO Water was prepared by adding 10 wt% BiSCaO to clean water…
View article: Bioshell Calcium Oxide (BiSCaO) Ointment for the Disinfection and Healing of Pseudomonas aeruginosa-Infected Wounds in Hairless Rats
Bioshell Calcium Oxide (BiSCaO) Ointment for the Disinfection and Healing of Pseudomonas aeruginosa-Infected Wounds in Hairless Rats Open
Bioshell calcium oxide (BiSCaO) possesses deodorizing properties and broad microbicidal activity. This study aimed to investigate the application of BiSCaO ointment for the prevention and treatment of infection in chronic wounds in healing…
View article: Ultraviolet Irradiation Enhances the Microbicidal Activity of Silver Nanoparticles by Hydroxyl Radicals
Ultraviolet Irradiation Enhances the Microbicidal Activity of Silver Nanoparticles by Hydroxyl Radicals Open
It is known that silver has microbicidal qualities; even at a low concentration, silver is active against many kinds of bacteria. Silver nanoparticles (AgNPs) have been extensively studied for a wide range of applications. Alternately, the…
View article: Skin Cleansing Technique with Disinfectant using Improved High-Velocity Steam-Air Micromist Jet Spray
Skin Cleansing Technique with Disinfectant using Improved High-Velocity Steam-Air Micromist Jet Spray Open
Application of a high-velocity steam-air micromist jet spray (HVS-AMJS) with disinfectants for cleansing the skin is proposed. Low-pressure steam is mixed with compressed air in a nozzle and then sprayed onto a surface of skin or material …
View article: Heparinoid Complex-Based Heparin-Binding Cytokines and Cell Delivery Carriers
Heparinoid Complex-Based Heparin-Binding Cytokines and Cell Delivery Carriers Open
Heparinoid is the generic term that is used for heparin, heparan sulfate (HS), and heparin-like molecules of animal or plant origin and synthetic derivatives of sulfated polysaccharides. Various biological activities of heparin/HS are attr…
View article: Application of Colloidal Dispersions of Bioshell Calcium Oxide (BiSCaO) for Disinfection
Application of Colloidal Dispersions of Bioshell Calcium Oxide (BiSCaO) for Disinfection Open
Bioshell calcium oxide (BiSCaO) is a scallop-shell powder heated at a high temperature. BiSCaO is composed mainly of calcium oxide and exhibits broad microbicidal properties. The aim of this study is to evaluate the disinfection and decont…
View article: Transplacental Gene Delivery (TPGD) as a Noninvasive Tool for Fetal Gene Manipulation in Mice
Transplacental Gene Delivery (TPGD) as a Noninvasive Tool for Fetal Gene Manipulation in Mice Open
Transplacental gene delivery (TPGD) is a technique for delivering nucleic acids to fetal tissues via tail-vein injections in pregnant mice. After transplacental transport, administered nucleic acids enter fetal circulation and are distribu…
View article: Preparation and Application of Bioshell Calcium Oxide (BiSCaO) Nanoparticle-Dispersions with Bactericidal Activity
Preparation and Application of Bioshell Calcium Oxide (BiSCaO) Nanoparticle-Dispersions with Bactericidal Activity Open
Scallop-shell powder (SSP) heated at high temperature exhibits high pH and broad antimicrobial activity. Bioshell calcium oxide (BiSCaO) is an SSP composed mainly of calcium oxide. It is poorly water-soluble under alkaline conditions and t…
View article: Synthesis and Application of Silver Nanoparticles (Ag NPs) for the Prevention of Infection in Healthcare Workers
Synthesis and Application of Silver Nanoparticles (Ag NPs) for the Prevention of Infection in Healthcare Workers Open
Silver is easily available and is known to have microbicidal effect; moreover, it does not impose any adverse effects on the human body. The microbicidal effect is mainly due to silver ions, which have a wide antibacterial spectrum. Furthe…
View article: Polyelectrolyte Complexes of Natural Polymers and Their Biomedical Applications
Polyelectrolyte Complexes of Natural Polymers and Their Biomedical Applications Open
Polyelectrolyte complexes (PECs), composed of natural and biodegradable polymers, (such as positively charged chitosan or protamine and negatively charged glycosaminoglycans (GAGs)) have attracted attention as hydrogels, films, hydrocolloi…
View article: Transplacental delivery of genome editing components causes mutations in embryonic cardiomyocytes of mid‐gestational murine fetuses
Transplacental delivery of genome editing components causes mutations in embryonic cardiomyocytes of mid‐gestational murine fetuses Open
Genome editing, as exemplified by CRISPR/Cas9, is now recognized as a powerful tool for the engineering of endogenous target genes. It employs only two components, namely, Cas9 in the form of DNA, mRNA, or protein; and guide RNA (gRNA), wh…
View article: Comparison of Various Disinfectants on Bactericidal Activity Under Organic Matter Contaminated Environments
Comparison of Various Disinfectants on Bactericidal Activity Under Organic Matter Contaminated Environments Open
The bactericidal activity of heated bio-shell calcium oxide (BiSCaO) powder suspension (pH 12.4) , hypochlorous acid (HClO; pH 6), sodium hypochlorite (NaClO; pH 8) , povidone-iodine (Isodine solution®) , and chlorhexidine gluconate (Hibis…
View article: FGF-2-containing dalteparin/protamine nanoparticles (FGF-2&D/P NPs) ameliorate UV-induced skin photoaging in hairless mice
FGF-2-containing dalteparin/protamine nanoparticles (FGF-2&D/P NPs) ameliorate UV-induced skin photoaging in hairless mice Open
UVB exposure penetrates deeply into the dermis to alter skin barrier function, which is a primary factor in skin photoaging. We previously reported that dalteparin and protamine nanoparticles (D/P NPs) are effective carriers of FGF-2. This…
View article: Intravenous Delivery of piggyBac Transposons as a Useful Tool for Liver-Specific Gene-Switching
Intravenous Delivery of piggyBac Transposons as a Useful Tool for Liver-Specific Gene-Switching Open
Hydrodynamics-based gene delivery (HGD) is an efficient method for transfecting plasmid DNA into hepatocytes in vivo. However, the resulting gene expression is transient, and occurs in a non-tissue specific manner. The piggyBac (PB) transp…
View article: Development of Mucoadhesive Chitosan Derivatives for Use as Submucosal Injections
Development of Mucoadhesive Chitosan Derivatives for Use as Submucosal Injections Open
Endoscopic mucosal resection (EMR) and endoscopic submucosal dissection (ESD) have been used for surgical treatment of early gastric cancer. These endoscopic techniques require proper submucosal injections beneath the tumor to provide a su…
View article: Behavior of Nitrate-Nitrogen and Nitrite-Nitrogen in Drinking Water
Behavior of Nitrate-Nitrogen and Nitrite-Nitrogen in Drinking Water Open
Nitrate-nitrogen (NO3-N) and nitrite-nitrogen (NO2-N) are constituents of the nitrogen cycle. NO3-N is toxic to humans, primarily due to its reduction to NO2-N. In Japan, NO3-N and NO<…
View article: Protective effect of FGF-2 and low-molecular-weight heparin/protamine nanoparticles on radiation-induced healing-impaired wound repair in rats
Protective effect of FGF-2 and low-molecular-weight heparin/protamine nanoparticles on radiation-induced healing-impaired wound repair in rats Open
We examined the effectiveness of localized administration of fibroblast growth factor-2 containing low-molecular-weight heparin/protamine nanoparticles (FGF-2&LMWH/P NPs) on apoptosis in vivo and on healing of radiation-induced skin injury…
View article: Feasibility of improving platelet-rich plasma therapy by using chitosan with high platelet activation ability
Feasibility of improving platelet-rich plasma therapy by using chitosan with high platelet activation ability Open
Platelet-rich plasma (PRP) is blood plasma containing a high number of platelets that release growth factors for wound healing and tissue regeneration. In the present study, the feasibility of improving PRP therapy by using chitosan that e…