Massimiliano Lucidi
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View article: Editorial: Deciphering antimicrobial resistance: genetic insights and perspectives
Editorial: Deciphering antimicrobial resistance: genetic insights and perspectives Open
View article: Exploring the Potential of Phytocannabinoids Against Multidrug-Resistant Bacteria
Exploring the Potential of Phytocannabinoids Against Multidrug-Resistant Bacteria Open
The rapid emergence of multidrug-resistant (MDR) bacterial pathogens poses a critical threat to global health, creating an urgent need for novel antimicrobial agents. In this study, we evaluated a small library of natural and semisynthetic…
View article: The response to desiccation in <i>Acinetobacter baumannii</i>
The response to desiccation in <i>Acinetobacter baumannii</i> Open
The long-term resistance to desiccation on abiotic surfaces is a key determinant of the adaptive success of Acinetobacter baumannii as a healthcare-associated bacterial pathogen. Here, the cellular and molecular mechanisms enabling …
View article: Antibacterial Interactions of Ethanol-Dispersed Multiwalled Carbon Nanotubes with <i>Staphylococcus aureus</i> and <i>Pseudomonas aeruginosa</i>
Antibacterial Interactions of Ethanol-Dispersed Multiwalled Carbon Nanotubes with <i>Staphylococcus aureus</i> and <i>Pseudomonas aeruginosa</i> Open
Infectious diseases are acknowledged as one of the leading causes of death worldwide. Statistics show that the annual death toll caused by bacterial infections has reached 14 million, most of which are caused by drug-resistant strains. Bac…
View article: Red-Emitting Fluorophores with Tailored Properties for Microscopy and Nanoscopy
Red-Emitting Fluorophores with Tailored Properties for Microscopy and Nanoscopy Open
Extended series of red-emitting rhodamine dyes were synthesized and tested. This revealed the real factors determining the quality of STED (stimulated emission depletion) imaging, dye photostability in general, and stability of the corresp…
View article: Redundant essentiality of AsmA-like proteins in <i>Pseudomonas aeruginosa</i>
Redundant essentiality of AsmA-like proteins in <i>Pseudomonas aeruginosa</i> Open
The outer membrane (OM) is an essential structure of Gram-negative bacteria that provides mechanical strength and protection from large and/or hydrophobic toxic molecules, including many antibiotics. The OM is composed of glycerophospholip…
View article: Phage-mediated colistin resistance in Acinetobacter baumannii
Phage-mediated colistin resistance in Acinetobacter baumannii Open
Φ19606 is an unprecedented example of a self-transmissible phage vector implicated in the dissemination of colistin resistance.
View article: Insights into the Antibacterial Properties of Cancer Theranostic Zinc-Dopped Iron Oxide ZnxFe3-xO4 Nanoparticles
Insights into the Antibacterial Properties of Cancer Theranostic Zinc-Dopped Iron Oxide ZnxFe3-xO4 Nanoparticles Open
View article: Apparent Death in Desiccated Acinetobacter Baumannii: Implications for Infection Control
Apparent Death in Desiccated Acinetobacter Baumannii: Implications for Infection Control Open
View article: Expanding the microbiologist toolbox <i>via</i> new far-red-emitting dyes suitable for bacterial imaging
Expanding the microbiologist toolbox <i>via</i> new far-red-emitting dyes suitable for bacterial imaging Open
Our understanding of bacteria is increasingly dependent on our ability to visualize cellular processes at the single-cell level with high spatial and temporal resolution. Advances in fluorescence microscopy are accompanied by an increasing…
View article: Pathogenicity and virulence of <i>Acinetobacter baumannii</i> : Factors contributing to the fitness in healthcare settings and the infected host
Pathogenicity and virulence of <i>Acinetobacter baumannii</i> : Factors contributing to the fitness in healthcare settings and the infected host Open
Acinetobacter baumannii is a common cause of healthcare-associated infections and hospital outbreaks, particularly in intensive care units. Much of the success of A. baumannii relies on its genomic plasticity, which allows ra…
View article: Phage-mediated transmission of colistin resistance in Acinetobacter baumannii
Phage-mediated transmission of colistin resistance in Acinetobacter baumannii Open
Antimicrobial resistance is a global threat to human health, and Acinetobacter baumannii is a paradigmatic example of how rapidly bacteria become resistant to clinically relevant antimicrobials. The emergence of multidrug-resistant A. baum…
View article: Pathogenicity and virulence of <i>Acinetobacter baumannii</i>: Factors contributing to the fitness in healthcare settings and the infected host
Pathogenicity and virulence of <i>Acinetobacter baumannii</i>: Factors contributing to the fitness in healthcare settings and the infected host Open
Acinetobacter baumannii is a common cause of healthcare-associated infections and hospital outbreaks, particularly in intensive care units. Much of the success of A. baumannii relies on its genomic plasticity, which allows ra…
View article: Fighting bacterial pathogens with carbon nanotubes: focused review of recent progress
Fighting bacterial pathogens with carbon nanotubes: focused review of recent progress Open
Main effects of SWCNTs and MWCNTs on E. coli and S. aureus bacterial cells.
View article: Bio-physical mechanisms of dehydrating membranes of Acinetobacter baumannii linked to drought-resistance
Bio-physical mechanisms of dehydrating membranes of Acinetobacter baumannii linked to drought-resistance Open
View article: Effect of a Defective Clamp Loader Complex of DNA Polymerase III on Growth and SOS Response in Pseudomonas aeruginosa
Effect of a Defective Clamp Loader Complex of DNA Polymerase III on Growth and SOS Response in Pseudomonas aeruginosa Open
DNA polymerase III (Pol III) is the replicative enzyme in bacteria. It consists of three subcomplexes, the catalytic core, the β clamp, and the clamp loader. While this complex has been thoroughly characterized in the model organism Escher…
View article: Antibacterial alkylguanidino ureas: Molecular simplification approach, searching for membrane-based MoA
Antibacterial alkylguanidino ureas: Molecular simplification approach, searching for membrane-based MoA Open
View article: Genome diversity of domesticated Acinetobacter baumannii ATCC 19606T strains
Genome diversity of domesticated Acinetobacter baumannii ATCC 19606T strains Open
Acinetobacter baumannii has emerged as an important opportunistic pathogen worldwide, being responsible for large outbreaks for nosocomial infections, primarily in intensive care units. A. baumannii ATCC 19606 T is the species type strain,…
View article: A Highly Sensitive Luminescent Biosensor for the Microvolumetric Detection of the<i>Pseudomonas aeruginosa</i>Siderophore Pyochelin
A Highly Sensitive Luminescent Biosensor for the Microvolumetric Detection of the<i>Pseudomonas aeruginosa</i>Siderophore Pyochelin Open
The pyochelin (PCH) siderophore produced by the pathogenic bacterium Pseudomonas aeruginosa is an important virulence factor, acting as a growth promoter during infection. While strong evidence exists for PCH production in vivo
View article: Generation of Genetic Tools for Gauging Multiple-Gene Expression at the Single-Cell Level
Generation of Genetic Tools for Gauging Multiple-Gene Expression at the Single-Cell Level Open
Within a bacterial population, single cells can differently express some genes, even though they are genetically identical and experience the same chemical and physical stimuli. This phenomenon, known as phenotypic heterogeneity, is mainly…
View article: Growth Phase- and Desiccation-Dependent <i>Acinetobacter baumannii</i> Morphology: An Atomic Force Microscopy Investigation
Growth Phase- and Desiccation-Dependent <i>Acinetobacter baumannii</i> Morphology: An Atomic Force Microscopy Investigation Open
Acinetobacter baumannii has emerged as a major bacterial pathogen during the past three decades. The majority of the A. baumannii infections occur in hospitals and are caused by strains endowed with high desiccation tolerance…
View article: Referee report. For: Antibiotic Drug screening and Image Characterization Toolbox (A.D.I.C.T.): a robust imaging workflow to monitor antibiotic stress response in bacterial cells in vivo [version 1; peer review: 1 approved with reservations]
Referee report. For: Antibiotic Drug screening and Image Characterization Toolbox (A.D.I.C.T.): a robust imaging workflow to monitor antibiotic stress response in bacterial cells in vivo [version 1; peer review: 1 approved with reservations] Open
View article: SSNOMBACTER: A collection of scattering-type scanning near-field optical microscopy and atomic force microscopy images of bacterial cells
SSNOMBACTER: A collection of scattering-type scanning near-field optical microscopy and atomic force microscopy images of bacterial cells Open
Background In recent years, a variety of imaging techniques operating at nanoscale resolution have been reported. These techniques have the potential to enrich our understanding of bacterial species relevant to human health, such as antibi…
View article: Front Cover
Front Cover Open
Images of bacterial cells stained with KK114 dye and visualized with STED microscopy. On the monitor: large field of view of B. subtilis cells , the KK114 ball‐and‐stick model and the schematics of the STED setup. Different space‐filling r…
View article: Quantitative evaluation of ImageJ thresholding algorithms for microbial cell counting
Quantitative evaluation of ImageJ thresholding algorithms for microbial cell counting Open
Binarization is a key process in microscopy cell counting and cytometry analysis that is performed before segmentation to identify a cell within the background. We test the performances of 16 global and 9 local ImageJ thresholding algorith…
View article: Geometrical-optics approach to measure the optical density of bacterial cultures using a LED-based photometer
Geometrical-optics approach to measure the optical density of bacterial cultures using a LED-based photometer Open
We develop a suitable geometrical-optics approach and demonstrate that it is possible to measure the optical density (OD) of bacterial cultures using a light emitting diode (LED)-based photometer. We measure both attenuation and spot-size …
View article: New Shuttle Vectors for Real-Time Gene Expression Analysis in Multidrug-Resistant Acinetobacter Species: <i>In Vitro</i> and <i>In Vivo</i> Responses to Environmental Stressors
New Shuttle Vectors for Real-Time Gene Expression Analysis in Multidrug-Resistant Acinetobacter Species: <i>In Vitro</i> and <i>In Vivo</i> Responses to Environmental Stressors Open
The short-term adaptive response to environmental cues greatly contributes to the ecological success of bacteria, and profound alterations in bacterial gene expression occur in response to physical, chemical, and nutritional stresses. Bact…
View article: Geometrical-optics approach to increase the accuracy in LED-based photometers for point-of-care testing
Geometrical-optics approach to increase the accuracy in LED-based photometers for point-of-care testing Open
A geometrical-optics approach is proposed to increase the accuracy in photometric measurements, using a point-of-care testing (POCT) LED-based sensor. Due to stray-light effects, the measurement accuracy depends on the dimension of the CMO…
View article: Contribution of Active Iron Uptake to Acinetobacter baumannii Pathogenicity
Contribution of Active Iron Uptake to Acinetobacter baumannii Pathogenicity Open
Acinetobacter baumannii is an important nosocomial pathogen. Mechanisms that allow A. baumannii to cause human infection are still poorly understood.
View article: Label-based and label-free optical nanoscopy of pathogenic bacterial species
Label-based and label-free optical nanoscopy of pathogenic bacterial species Open