Manuel Simões
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View article: Physicochemical, Structural, and Proteomic Insights into Drinking Water-Isolated Acinetobacter calcoaceticus Aggregation and Biofilm Dynamics
Physicochemical, Structural, and Proteomic Insights into Drinking Water-Isolated Acinetobacter calcoaceticus Aggregation and Biofilm Dynamics Open
Acinetobacter calcoaceticus, a ubiquitous Gram-negative bacterium, exhibits remarkable adaptability across diverse environments, including drinking water distribution systems (DWDS), where its biofilm-forming and coaggregation capabilities…
View article: The role of methylparaben as an environmental contaminant in enhancing the pathogenicity of drinking water bacteria
The role of methylparaben as an environmental contaminant in enhancing the pathogenicity of drinking water bacteria Open
Parabens, commonly used as preservatives in personal care products, can affect drinking water (DW) environmental microbial communities, posing an emerging global concern for public health. This study examines the impact of MP at 15 µg/L on…
View article: Statement of Peer Review
Statement of Peer Review Open
In submitting conference proceedings to Medical Sciences Forum, the volume editors of the proceedings certify to the publisher that all papers published in this volume have been subjected to peer review administered by the volume editors […
View article: When Precision Matters: Bone Marrow Cytology Meets qPCR in a Pilot Study Quantifying Leishmania infantum Load in Dogs
When Precision Matters: Bone Marrow Cytology Meets qPCR in a Pilot Study Quantifying Leishmania infantum Load in Dogs Open
Leishmania infantum is the causative agent of canine leishmaniosis (CanL), a zoonotic disease of considerable clinical and epidemiological concern. Quantification of parasite load is valuable for clinical management, particularly in low-pa…
View article: AHL-based QS signalling promotes uropathogenic Escherichia coli settlement through the de-repression of biofilm formation by SdiA
AHL-based QS signalling promotes uropathogenic Escherichia coli settlement through the de-repression of biofilm formation by SdiA Open
Uropathogenic Escherichia coli (UPEC) are among the first pathogens to colonise in catheter and non-catheter-associated urinary tract infections. However, these infections are often polymicrobial, resulting in multi-species infections that…
View article: Eradicating microplastics in wastewater: microalgae as a sustainable strategy
Eradicating microplastics in wastewater: microalgae as a sustainable strategy Open
Microplastics (MPs) are pervasive environmental pollutants that have infiltrated ecosystems worldwide due to their small size and persistence. Although wastewater treatment plants (WWTPs) can achieve up to 90 % removal of MPs, this is insu…
View article: Methylparaben as an environmental contaminant modulating virulence traits in waterborne bacteria
Methylparaben as an environmental contaminant modulating virulence traits in waterborne bacteria Open
Methylparaben (MP), a preservative found in daily-use products, can reach drinking water (DW) due to its incomplete removal in water treatment plants. Although parabens are related to endocrine disruption, their effects on bacterial virule…
View article: Innovative and high efficiency harvesting process of Chlorella vulgaris by magnetic harvesting
Innovative and high efficiency harvesting process of Chlorella vulgaris by magnetic harvesting Open
This study explored the use of magnetic flocculation to harvest Chlorella vulgaris from synthetic wastewater by combining polyaluminium chloride (PAC), polyacrylamide (PAM), and Fe3O4 magnetic particles. The approach focused on finding the…
View article: Abstracts of the 4th International Electronic Conference on Antibiotics
Abstracts of the 4th International Electronic Conference on Antibiotics Open
The 4th International Electronic Conference on Antibiotics (ECA2025) was an online conference held on 21–23 May 2025, organized by the MDPI journal Antibiotics. This event aims to bring together researchers and scholars to discuss a wide r…
View article: Qualitative Assessment of Microalgae–Bacteria Biofilm Development on K5 Carriers: Photoheterotrophic Growth in Wastewater
Qualitative Assessment of Microalgae–Bacteria Biofilm Development on K5 Carriers: Photoheterotrophic Growth in Wastewater Open
Wastewater (WW) treatment using biofilms harboring bacteria and microalgae is considered a promising polishing solution to improve current treatment technologies present in wastewater treatment plants (WWTPs), but their interaction in a se…
View article: Two cinnamic acid derivatives as inhibitors of Pseudomonas aeruginosa las and pqs quorum-sensing systems: Impact on biofilm formation and virulence factors
Two cinnamic acid derivatives as inhibitors of Pseudomonas aeruginosa las and pqs quorum-sensing systems: Impact on biofilm formation and virulence factors Open
Ferulic and sinapic acids compromised the biofilm architecture and virulence of P. aeruginosa through QS inhibition.
View article: Current microbiological challenges in drinking water
Current microbiological challenges in drinking water Open
Drinking water (DW) is paramount to human health, serving as a cornerstone of public health worldwide. However, DW is not a sterile product and can harbor a large diversity of microorganisms, including pathogens. This comprehensive review …
View article: Influence of bacterial load on the development and performance of a photoheterotrophic biofilm community for wastewater polishing
Influence of bacterial load on the development and performance of a photoheterotrophic biofilm community for wastewater polishing Open
A novel photoheterotrophic dual-species biofilm was evaluated for wastewater (WW) treatment and microbial growth under variable cell loads. For that, Chlorella vulgaris was tested under axenic conditions and in combination with three bacte…
View article: The ability of <i>Salmonella enterica</i> subsp. <i>enterica</i> strains to form biofilms on abiotic surfaces and their susceptibility to selected essential oil components
The ability of <i>Salmonella enterica</i> subsp. <i>enterica</i> strains to form biofilms on abiotic surfaces and their susceptibility to selected essential oil components Open
The ability of Salmonella enterica subsp. enterica to persist and form biofilms on different surfaces can constitute a source of food contamination, being an issue of global concern. The objective of this study was to understand the biofil…
View article: New insights on antibacterial mode of action of blue-light photoactivated berberine and curcumin-antibiotic combinations against Staphylococcus aureus
New insights on antibacterial mode of action of blue-light photoactivated berberine and curcumin-antibiotic combinations against Staphylococcus aureus Open
Antimicrobial photodynamic inactivation (aPDI), using photosensitisers in combination with antibiotics, is a promising multi-target strategy to address antibiotic resistance, particularly in wound infections. This study aimed to elucidate …
View article: The influence of photoperiod and organic carbon levels in parabens removal from wastewater by Chlorella vulgaris
The influence of photoperiod and organic carbon levels in parabens removal from wastewater by Chlorella vulgaris Open
Parabens are emerging contaminants due to their abundant use as preservatives and inefficient treatment in wastewater (WW) treatment plants. To overcome the limitations of WW treatment plants in removing parabens, microalgae-based bioremed…
View article: Unravelling the potential of natural chelating agents in the control of Staphylococcus aureus and Pseudomonas aeruginosa biofilms
Unravelling the potential of natural chelating agents in the control of Staphylococcus aureus and Pseudomonas aeruginosa biofilms Open
Iron is essential for the formation, maturation and dispersal of bacterial biofilms, playing a crucial role in the physiological and metabolic functions of bacteria as well as in the regulation of virulence. Limited availability of iron ca…
View article: Parabens alter the surface characteristics and antibiotic susceptibility of drinking water bacteria
Parabens alter the surface characteristics and antibiotic susceptibility of drinking water bacteria Open
Parabens are markedly present in products of daily use, considered emerging environmental contaminants that can harm human health and aquatic life, due to their release into aquatic sources. The impact of the exposure of microbial communit…
View article: Track of methylparaben in the bulk phase and on the extracellular matrix of dual-species biofilms: Biodegradation and bioaccumulation
Track of methylparaben in the bulk phase and on the extracellular matrix of dual-species biofilms: Biodegradation and bioaccumulation Open
Methylparaben (MP) is a preservative considered an environmental contaminant of emerging concern due to its persistence in water sources, including drinking water (DW). This study assesses the interaction between MP and dual-species biofil…
View article: Correction: Correia et al. Beyond Penicillin: The Potential of Filamentous Fungi for Drug Discovery in the Age of Antibiotic Resistance. 2023, 12, 1250
Correction: Correia et al. Beyond Penicillin: The Potential of Filamentous Fungi for Drug Discovery in the Age of Antibiotic Resistance. 2023, 12, 1250 Open
Manuel Simões was included as a corresponding author in the original publication [...]
View article: Protocol for in silico characterization of natural-based molecules as quorum-sensing inhibitors
Protocol for in silico characterization of natural-based molecules as quorum-sensing inhibitors Open
The search and development of new quorum-sensing (QS) inhibitors are ongoing processes for biofilm control. Here, we present a protocol for in silico characterization of natural-based molecules as QS inhibitors. We describe steps for prepa…
View article: Coaggregation dynamics in drinking water biofilms and implications for chlorine disinfection
Coaggregation dynamics in drinking water biofilms and implications for chlorine disinfection Open
Biofilms in drinking water (DW) systems persistently challenge traditional disinfection methods due to intricate microbial interactions, with coaggregation playing a crucial role in forming multispecies biofilms. This study examined the im…
View article: The impact of methylparaben and chlorine on the architecture of Stenotrophomonas maltophilia biofilms
The impact of methylparaben and chlorine on the architecture of Stenotrophomonas maltophilia biofilms Open
The biofilm architecture is significantly influenced by external environmental conditions. Biofilms grown on drinking water distribution systems (DWDS) are exposed to environmental contaminants, including parabens, and disinfection strateg…