Mathew Milner
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View article: High-throughput chemical genomic screening: a step-by-step workflow from plate to phenotype
High-throughput chemical genomic screening: a step-by-step workflow from plate to phenotype Open
High-throughput chemical genomics uses phenotypic profiling of strain libraries under defined chemical and environmental conditions to identify gene functions. This approach enables the mapping of biological pathways and can potentially hi…
View article: High-Throughput Chemical Genomic Screening: A Step-by-Step Workflowfrom Plate to Phenotype
High-Throughput Chemical Genomic Screening: A Step-by-Step Workflowfrom Plate to Phenotype Open
High-throughput chemical genomics uses phenotypic profiling of strain libraries under defined chemical and environmental conditions to identify gene functions. This approach enables the mapping of biological pathways and can potentially hi…
View article: Methicillin-resistant Staphylococcus aureus in Saudi Arabia: genomic evidence of recent clonal expansion and plasmid-driven resistance dissemination
Methicillin-resistant Staphylococcus aureus in Saudi Arabia: genomic evidence of recent clonal expansion and plasmid-driven resistance dissemination Open
Objectives Staphylococcus aureus is a leading cause of hospital-acquired infections worldwide. Over recent decades, methicillin-resistant Staphylococcus aureus (MRSA), which is resistant to multiple antimicrobials, has emerged as a signifi…
View article: Methicillin-resistant<i>Staphylococcus aureus</i>in Saudi Arabia: genomic evidence of recent clonal expansion and plasmid-driven resistance dissemination
Methicillin-resistant<i>Staphylococcus aureus</i>in Saudi Arabia: genomic evidence of recent clonal expansion and plasmid-driven resistance dissemination Open
Objectives Staphylococcus aureus is a leading cause of hospital-acquired infections worldwide. Over recent decades, methicillin-resistant Staphylococcus aureus (MRSA), which is resistant to multiple antimicrobials, has emerged as a signifi…
View article: The dissemination of multidrug-resistant and hypervirulent <i>Klebsiella pneumoniae</i> clones across the Kingdom of Saudi Arabia
The dissemination of multidrug-resistant and hypervirulent <i>Klebsiella pneumoniae</i> clones across the Kingdom of Saudi Arabia Open
Klebsiella pneumoniae is a Gram-negative bacterium associated with a wide range of community- and hospital-acquired infections. The emergence of clonal hypervirulent strains resistant to last-resort antimicrobial agents has become a global…
View article: Bam complex associated proteins in Escherichia coli are functionally linked to peptidoglycan biosynthesis, membrane fluidity and DNA replication
Bam complex associated proteins in Escherichia coli are functionally linked to peptidoglycan biosynthesis, membrane fluidity and DNA replication Open
Biogenesis of the bacterial outer membrane is key to bacterial survival and antibiotic resistance. Central to this is the β-barrel assembly machine (Bam) complex and its associated chaperones, which are responsible for transport, folding a…
View article: Bam complex associated proteins in Escherichia coli are functionally linked to peptidoglycan biosynthesis, membrane fluidity and DNA replication
Bam complex associated proteins in Escherichia coli are functionally linked to peptidoglycan biosynthesis, membrane fluidity and DNA replication Open
Biogenesis of the bacterial outer membrane is key to bacterial survival and antibiotic resistance. Central to this is the β-barrel assembly machine (Bam) complex and its associated chaperones, which are responsible for transport, folding a…
View article: Large-scale Genomic Survey of Non-typhoidal<i>Salmonella enterica</i>serovar Minnesota Strains in Chicken Products Reveals the Emergence of Multidrug Resistant Clones
Large-scale Genomic Survey of Non-typhoidal<i>Salmonella enterica</i>serovar Minnesota Strains in Chicken Products Reveals the Emergence of Multidrug Resistant Clones Open
Background Salmonella enterica serovar Minnesota ( S . Minnesota) is an emerging serovar of non-typhoidal Salmonella , known to persist in the food chain and distribution systems, potentially leading to outbreaks of Salmonella infections i…
View article: The Dissemination of Multidrug-Resistant and Hypervirulent<i>Klebsiella pneumoniae</i>Clones Across the Kingdom of Saudi Arabia
The Dissemination of Multidrug-Resistant and Hypervirulent<i>Klebsiella pneumoniae</i>Clones Across the Kingdom of Saudi Arabia Open
The Gram-negative bacterium Klebsiella pneumoniae is a major human health threat underlying a broad range of community- and hospital-associated infections. The emergence of clonal hypervirulent strains often resistant to last-resort antimi…
View article: Bam complex associated proteins in <i>Escherichia coli</i> are functionally linked to peptidoglycan biosynthesis, membrane fluidity and DNA replication
Bam complex associated proteins in <i>Escherichia coli</i> are functionally linked to peptidoglycan biosynthesis, membrane fluidity and DNA replication Open
Biogenesis of the bacterial outer membrane is key to bacterial survival and antibiotic resistance. Central to this is the β-barrel assembly machine (Bam) complex and its associated chaperones, which are responsible for transport, folding a…
View article: Use of Transposon Directed Insertion-Site Sequencing to Probe the Antibacterial Mechanism of a Model Honey on E. coli K-12
Use of Transposon Directed Insertion-Site Sequencing to Probe the Antibacterial Mechanism of a Model Honey on E. coli K-12 Open
Antimicrobial resistance is an ever-growing health concern worldwide that has created renewed interest in the use of traditional anti-microbial treatments, including honey. However, understanding the underlying mechanism of the anti-microb…
View article: Transposon Directed Insertion-site Sequencing of a BW25113 ascA (yecA) deletion mutant
Transposon Directed Insertion-site Sequencing of a BW25113 ascA (yecA) deletion mutant Open
We carried out saturated transposon mutagenesis of an ascA deletion mutant, determined the location of the transposon insertions using Illumina sequencing and mapped them to the BW25113 reference genome (CP009273.1). We have deposited thre…
View article: High throughput approaches to study laboratory-based evolution of E. coli for enhanced growth at low pH
High throughput approaches to study laboratory-based evolution of E. coli for enhanced growth at low pH Open
Laboratory-based evolution has become a widely used method to explore fundamental questions about evolution as a process, and is also a powerful tool to study the link between genotype and phenotype. We have evolved six populations of E. c…
View article: The role of neuronal nitric oxide and its pathways in the protection and recovery from neurotoxin-induced <em>de novo</em> hypokinetic motor behaviors in the embryonic zebrafish (<em>Danio rerio</em>)
The role of neuronal nitric oxide and its pathways in the protection and recovery from neurotoxin-induced <em>de novo</em> hypokinetic motor behaviors in the embryonic zebrafish (<em>Danio rerio</em>) Open
Neuronal nitric oxide (nNO) has been shown to affect motor function in the brain. Specifically, nNO acts in part through regulation of dopamine (DA) release, transporter function, and the elicitation of neuroprotection/neurodegeneration of…