Maher Abou Hachem
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View article: A single residue affects the dynamics and shape of a tetrameric <scp>GH43</scp> β‐1,4‐<scp>d</scp>‐xylosidase from <i>Levilactobacillus brevis</i><scp>DSM1269</scp>
A single residue affects the dynamics and shape of a tetrameric <span>GH43</span> β‐1,4‐<span>d</span>‐xylosidase from <i>Levilactobacillus brevis</i><span>DSM1269</span> Open
Lignocellulosic materials (e.g., straw and bran) are gaining interest as feedstocks for the manufacture of higher value products, recognizing xylooligosaccharides (XOS) as interesting prebiotic compounds, and putting enzymes converting xyl…
View article: Dual human milk oligosaccharide-fibre utilisation drives gut microbiome selection during weaning
Dual human milk oligosaccharide-fibre utilisation drives gut microbiome selection during weaning Open
Gut microbiome (GM) maturation in early life follows organised taxonomic successions. How weaning impacts these trajectories remains underexplored. Here, we sampled faeces from seven mother-infant dyads at pre-, early and late weaning. Enr…
View article: Cladosporium species detoxify multiple water micropollutants of emerging concern
Cladosporium species detoxify multiple water micropollutants of emerging concern Open
The accumulation of micropollutants of emerging concern in aqueous ecological niches raises safety concerns regarding biological systems and human health. Mycoremediation is a promising and green strategy to mitigate the micropollutant cha…
View article: Uptake of fucosylated type I human milk oligosaccharide blocks by <i>Bifidobacterium longum</i> subsp. <i>infantis</i>
Uptake of fucosylated type I human milk oligosaccharide blocks by <i>Bifidobacterium longum</i> subsp. <i>infantis</i> Open
Human milk oligosaccharides (HMOs) are uniquely rich in the type 1 building block disaccharide lacto- N -biose I (LNB; Galβ1,3GlcNAc), as compared to other mammals. Most HMOs are fucosylated, for example, α1,2 and α1,4 fucosylations on LNB…
View article: Uptake of fucosylated type I human milk oligosaccharide blocks by<i>Bifidobacterium longum</i>subsp.<i>infantis</i>
Uptake of fucosylated type I human milk oligosaccharide blocks by<i>Bifidobacterium longum</i>subsp.<i>infantis</i> Open
Human milk oligosaccharides (HMOs) are uniquely rich in the type 1 building block disaccharide lacto- N -biose I (LNB, Galβ1,3GlcNAc), as compared to other mammals. Most HMOs are fucosylated, e.g. , α1,2 and α1,4 fucosylations on LNB block…
View article: Tailor-Made α-Glucans by Engineering the Processivity of α-Glucanotransferases via Tunnel-Cleft Active Center Interconversions
Tailor-Made α-Glucans by Engineering the Processivity of α-Glucanotransferases via Tunnel-Cleft Active Center Interconversions Open
The function of polysaccharides is intimately associated with their size, which is largely determined by the processivity of transferases responsible for their synthesis. A tunnel active center architecture has been recognized as a key fac…
View article: Insights into the Structure–Function Relationship of GH70 GtfB α-Glucanotransferases from the Crystal Structure and Molecular Dynamic Simulation of a Newly Characterized <i>Limosilactobacillus reuteri</i> N1 GtfB Enzyme
Insights into the Structure–Function Relationship of GH70 GtfB α-Glucanotransferases from the Crystal Structure and Molecular Dynamic Simulation of a Newly Characterized <i>Limosilactobacillus reuteri</i> N1 GtfB Enzyme Open
α-Glucanotransferases of the CAZy family GH70 convert starch-derived donors to industrially important α-glucans. Here, we describe characteristics of a novel GtfB-type 4,6-α-glucanotransferase of high enzyme activity (60.8 U mg-1) from Lim…
View article: <i>Cladosporium</i>species detoxify multiple water micropollutants of emerging concern using diverse strategies
<i>Cladosporium</i>species detoxify multiple water micropollutants of emerging concern using diverse strategies Open
The accumulation of micropollutants of emerging concern in aqueous systems raises safety concerns regarding biological systems and human health. Mycoremediation is a promising and green strategy to mitigate the micropollutant challenge. Hi…
View article: Sialidases and fucosidases of Akkermansia muciniphila are crucial for growth on mucin and nutrient sharing with mucus-associated gut bacteria
Sialidases and fucosidases of Akkermansia muciniphila are crucial for growth on mucin and nutrient sharing with mucus-associated gut bacteria Open
The mucolytic human gut microbiota specialist Akkermansia muciniphila is proposed to boost mucin-secretion by the host, thereby being a key player in mucus turnover. Mucin glycan utilization requires the removal of protective caps, notably…
View article: Evaluation of Enzymatic Hydrolysis of Sugarcane Bagasse Using Combination of Enzymes or Co-Substrate to Boost Lytic Polysaccharide Monooxygenases Action
Evaluation of Enzymatic Hydrolysis of Sugarcane Bagasse Using Combination of Enzymes or Co-Substrate to Boost Lytic Polysaccharide Monooxygenases Action Open
This study evaluated innovative approaches for the enzymatic hydrolysis of lignocellulosic biomass. More specifically, assays were performed to evaluate the supplementation of the commercial cellulolytic cocktail Cellic® CTec2 (CC2) with L…
View article: Sialidases and Fucosidases of <i>Akkermansia muciniphila</i> are crucial for growth on mucin and nutrient sharing with mucus-associated gut bacteria
Sialidases and Fucosidases of <i>Akkermansia muciniphila</i> are crucial for growth on mucin and nutrient sharing with mucus-associated gut bacteria Open
The gut mucolytic specialist Akkermansia muciniphila is strongly associated with the integrity of the mucus layer. Mucin glycan utilization requires the removal of diverse protective caps, notably, fucose and sialic acid, but the enzymatic…
View article: Generation of Multivalent Nanobody-Based Proteins with Improved Neutralization of Long α-Neurotoxins from Elapid Snakes
Generation of Multivalent Nanobody-Based Proteins with Improved Neutralization of Long α-Neurotoxins from Elapid Snakes Open
Recombinantly produced biotherapeutics hold promise for improving the current standard of care for snakebite envenoming over conventional serotherapy. Nanobodies have performed well in the clinic, and in the context of antivenom, they have…
View article: Engineering Bifidobacterium longum Endo-α-N-acetylgalactosaminidase for Neu5Acα2-3Galβ1-3GalNAc reactivity on Fetuin
Engineering Bifidobacterium longum Endo-α-N-acetylgalactosaminidase for Neu5Acα2-3Galβ1-3GalNAc reactivity on Fetuin Open
Endo-α-N-acetylgalactosaminidase from Bifidobacterium longum (EngBF) belongs to the glycoside hydrolase family GH101 and has a strict preference towards the mucin type glycan, Galβ1-3GalNAc, which is O-linked to serine or threonine residue…
View article: Diversification of a Fucosyllactose Transporter within the Genus <i>Bifidobacterium</i>
Diversification of a Fucosyllactose Transporter within the Genus <i>Bifidobacterium</i> Open
Human milk oligosaccharides (HMOs), which are natural bifidogenic prebiotics, were recently commercialized to fortify formula milk. However, HMO assimilation phenotypes of bifidobacteria vary by species and strain, which has not been fully…
View article: Structure and evolution of the bifidobacterial carbohydrate metabolism proteins and enzymes
Structure and evolution of the bifidobacterial carbohydrate metabolism proteins and enzymes Open
Bifidobacteria have attracted significant attention because they provide health-promoting effects in the human gut. In this review, we present a current overview of the three-dimensional structures of bifidobacterial proteins involved in c…
View article: Activity-Based Protein Profiling of Retaining α-Amylases in Complex Biological Samples
Activity-Based Protein Profiling of Retaining α-Amylases in Complex Biological Samples Open
Amylases are key enzymes in the processing of starch in many kingdoms of life. They are important catalysts in industrial biotechnology where they are applied in, among others, food processing and the production of detergents. In man amyla…
View article: Discovery of fungal oligosaccharide-oxidising flavo-enzymes with previously unknown substrates, redox-activity profiles and interplay with LPMOs
Discovery of fungal oligosaccharide-oxidising flavo-enzymes with previously unknown substrates, redox-activity profiles and interplay with LPMOs Open
Oxidative plant cell-wall processing enzymes are of great importance in biology and biotechnology. Yet, our insight into the functional interplay amongst such oxidative enzymes remains limited. Here, a phylogenetic analysis of the auxiliar…
View article: An 1,4-α-Glucosyltransferase Defines a New Maltodextrin Catabolism Scheme in Lactobacillus acidophilus
An 1,4-α-Glucosyltransferase Defines a New Maltodextrin Catabolism Scheme in Lactobacillus acidophilus Open
The degradation of starch in the small intestine generates short linear and branched α-glucans. The latter are poorly digestible by humans, rendering them available to the gut microbiota, e.g., lactobacilli adapted to the small intestine a…
View article: Butyrate producing Clostridiales utilize distinct human milk oligosaccharides correlating to early colonization and prevalence in the human gut
Butyrate producing Clostridiales utilize distinct human milk oligosaccharides correlating to early colonization and prevalence in the human gut Open
The early life human gut microbiota exerts life-long health effects on the host, but the mechanisms underpinning its assembly remain elusive. Particularly, the early colonization of Clostridiales from the Roseburia-Eubacterium group, assoc…
View article: An 1,4-α-glucosyltransferase defines a new maltodextrin catabolism scheme in<i>Lactobacillus acidophilus</i>
An 1,4-α-glucosyltransferase defines a new maltodextrin catabolism scheme in<i>Lactobacillus acidophilus</i> Open
The maltooligosaccharide (MOS) utilization locus in Lactobacillus acidophilus NCFM, a model for human small-intestine lactobacilli, encodes a family 13 subfamily 31 glycoside hydrolase (GH13_31), annotated as an 1,6-α-glucosidase. Here, we…
View article: The Catalytic Acid–Base in GH109 Resides in a Conserved GGHGG Loop and Allows for Comparable α-Retaining and β-Inverting Activity in an <i>N</i> -Acetylgalactosaminidase from <i>Akkermansia muciniphila</i>
The Catalytic Acid–Base in GH109 Resides in a Conserved GGHGG Loop and Allows for Comparable α-Retaining and β-Inverting Activity in an <i>N</i> -Acetylgalactosaminidase from <i>Akkermansia muciniphila</i> Open
Enzymes active on glycosidic bonds are defined according to the stereochemistry of both substrates and products of the reactions they catalyze. The CAZy classification further assigns these enzymes into sequence-based families sharing a co…
View article: Additional file 2 of Loss of AA13 LPMOs impairs degradation of resistant starch and reduces the growth of Aspergillus nidulans
Additional file 2 of Loss of AA13 LPMOs impairs degradation of resistant starch and reduces the growth of Aspergillus nidulans Open
Additional file 2: Label-free Proteomics.
View article: Molecular insight into a new low‐affinity xylan binding module from the xylanolytic gut symbiont <i>Roseburia intestinalis</i>
Molecular insight into a new low‐affinity xylan binding module from the xylanolytic gut symbiont <i>Roseburia intestinalis</i> Open
Efficient capture of glycans, the prime metabolic resources in the human gut, confers a key competitive advantage for gut microbiota members equipped with extracellular glycoside hydrolases (GHs) to target these substrates. The association…
View article: The Catalytic Acid-Base in GH109 Resides in a Conserved GGHGG Loop and Allows for Comparable α-Retaining and β-Inverting Activity in an N-Acetylgalactosaminidase from Akkermansia Muciniphila
The Catalytic Acid-Base in GH109 Resides in a Conserved GGHGG Loop and Allows for Comparable α-Retaining and β-Inverting Activity in an N-Acetylgalactosaminidase from Akkermansia Muciniphila Open
The study describes the first glycoside hydrolase that exhibits comparable levels of activity on α- and β-linked saccharide substrates. This enzyme, assigned into GH109, is encoded by the genome of the human gut symbiont Akkermansia mucini…