Mathias Winterhalter
YOU?
Author Swipe
View article: Aetokthonotoxin, the Causative Agent of Vacuolar Myelinopathy, Uncouples Oxidative Phosphorylation due to Protonophore Activity
Aetokthonotoxin, the Causative Agent of Vacuolar Myelinopathy, Uncouples Oxidative Phosphorylation due to Protonophore Activity Open
Aetokthonotoxin (AETX) is an emerging environmental toxin produced by the freshwater cyanobacterium Aetokthonos hydrillicola. Accumulating in the food chain, it causes vacuolar myelinopathy, a neurological disease affecting a wide range of…
View article: Supramolecular Ion Channels to Engineer Zn<sup>2+</sup> Ion Transport Mediated Chemical‐to‐Optical Signal Transduction
Supramolecular Ion Channels to Engineer Zn<sup>2+</sup> Ion Transport Mediated Chemical‐to‐Optical Signal Transduction Open
Artificial ion channels have significant potential for various applications, including molecular communication, bio‐sensing, and constructing artificial cells. In this report, we demonstrated the development of a molecular communication sy…
View article: Highly potent quinoxalinediones inhibit α-hemolysin and ameliorate Staphylococcus aureus lung infections
Highly potent quinoxalinediones inhibit α-hemolysin and ameliorate Staphylococcus aureus lung infections Open
Hospital-acquired pneumonia caused by Staphylococcus aureus is associated with patient morbidity and mortality, despite adequate antibiotic therapy. This illustrates the need for treatments beyond antibiotics. The pore-forming heptameric t…
View article: Aetokthonotoxin, the causative agent of vacuolar myelinopathy, uncouples oxidative phosphorylation due to protonophore activity
Aetokthonotoxin, the causative agent of vacuolar myelinopathy, uncouples oxidative phosphorylation due to protonophore activity Open
Aetokthonotoxin (AETX) is an emerging environmental toxin produced by the freshwater cyanobacterium Aetokthonos hydrillicola . Accumulating in the food chain, it causes vacuolar myelinopathy, a neurological disease affecting a wide range o…
View article: Vesicle‐Encapsulated Chemosensing Ensembles Allow Monitoring of Transmembrane Uptake Coupled with Enzymatic Reactions
Vesicle‐Encapsulated Chemosensing Ensembles Allow Monitoring of Transmembrane Uptake Coupled with Enzymatic Reactions Open
Compartmentalized models with coupled catalytic networks are considered as “protocells” in the context of research related to the origin of life. To model the kinetics of a simple cellular uptake‐metabolism process, we use a compartmentali…
View article: Vesicle‐Encapsulated Chemosensing Ensembles Allow Monitoring of Transmembrane Uptake Coupled with Enzymatic Reactions
Vesicle‐Encapsulated Chemosensing Ensembles Allow Monitoring of Transmembrane Uptake Coupled with Enzymatic Reactions Open
Compartmentalized models with coupled catalytic networks are considered as “protocells” in the context of research related to the origin of life. To model the kinetics of a simple cellular uptake‐metabolism process, we use a compartmentali…
View article: Single-Molecule Sensing inside a Stereo- and Regiodefined Hetero-Nanopore
Single-Molecule Sensing inside a Stereo- and Regiodefined Hetero-Nanopore Open
Channel forming proteins often contain recognition pattern or stereo specific selection filter. This ability of the channel confinement inspired us for application towards single molecule sensing. To bypass possible difficulties in purific…
View article: Conformational flexibility driving charge-selective substrate translocation across a bacterial transporter
Conformational flexibility driving charge-selective substrate translocation across a bacterial transporter Open
Here, we establish the conformational dynamics of a specialized natural passive bacterial transporter, CymA, using single-channel recordings and molecular dynamics simulations.
View article: Fosfomycin Uptake in <i>Escherichia coli</i> Is Mediated by the Outer-Membrane Porins OmpF, OmpC, and LamB
Fosfomycin Uptake in <i>Escherichia coli</i> Is Mediated by the Outer-Membrane Porins OmpF, OmpC, and LamB Open
The antibiotic fosfomycin (FOS) is widely recognized for the treatment of lower urinary tract infections with Escherichia coli and has lately gained importance as a therapeutic option to combat multidrug-resistant bacteria. However, resist…
View article: Metallacarborane Cluster Anions of the Cobalt Bisdicarbollide-Type as Chaotropic Carriers for Transmembrane and Intracellular Delivery of Cationic Peptides
Metallacarborane Cluster Anions of the Cobalt Bisdicarbollide-Type as Chaotropic Carriers for Transmembrane and Intracellular Delivery of Cationic Peptides Open
Cobalt bisdicarbollides (COSANs) are inorganic boron-based anions that have been previously reported to permeate by themselves through lipid bilayer membranes, a propensity that is related to their superchaotropic character. We now introdu…
View article: Influence of Membrane Asymmetry on OmpF Insertion, Orientation and Function
Influence of Membrane Asymmetry on OmpF Insertion, Orientation and Function Open
The effect of asymmetric membranes containing lipopolysaccharides (LPS) on the outer membrane protein F (OmpF) reconstitution, channel orientation, and antibiotic permeation across the outer membrane was investigated. After forming an asym…
View article: Editorial: Nanopore Electrochemistry
Editorial: Nanopore Electrochemistry Open
This Special Collection highlights the most recent developments in nanopore electrochemistry and applications. In this Editorial, guest Editors Yi-Tao Long, Meni Wanunu, and Mathias Winterhalter briefly introduce the research published in …
View article: How the physical properties of bacterial porins match environmental conditions
How the physical properties of bacterial porins match environmental conditions Open
Despite the high homology of OmpF and OmpC, the internally folded loop responds differently to temperature increase.
View article: Cephalosporin translocation across enterobacterial OmpF and OmpC channels, a filter across the outer membrane
Cephalosporin translocation across enterobacterial OmpF and OmpC channels, a filter across the outer membrane Open
Gram-negative porins are the main entry for small hydrophilic molecules. We studied translocation of structurally related cephalosporins, ceftazidime (CAZ), cefotaxime (CTX) and cefepime (FEP). CAZ is highly active on E. coli producing Omp…
View article: Uptake of aminoglycosides through outer membrane porins in <i>Escherichia coli</i>
Uptake of aminoglycosides through outer membrane porins in <i>Escherichia coli</i> Open
Aminoglycosides are important clinical antibiotics but their molecular uptake mechanism is still not completely understood. Here we quantify and compare the passive transport of three aminoglycosides (kanamycin, gentamicin, and amikacin) a…
View article: Dataset for Conformational dynamics of loop L3 in OmpF: Implications towards antibiotic translocation and voltage gating
Dataset for Conformational dynamics of loop L3 in OmpF: Implications towards antibiotic translocation and voltage gating Open
Dataset supporting the work in the publication titled, "Conformational dynamics of loop L3 in OmpF: Implications towards antibiotic translocation and voltage gating".
View article: Dataset for Conformational dynamics of loop L3 in OmpF: Implications towards antibiotic translocation and voltage gating
Dataset for Conformational dynamics of loop L3 in OmpF: Implications towards antibiotic translocation and voltage gating Open
Dataset supporting the work in the publication titled, "Conformational dynamics of loop L3 in OmpF: Implications towards antibiotic translocation and voltage gating".
View article: Identification of Single Amino Acid Chiral and Positional Isomers Using an Electrostatically Asymmetric Nanopore
Identification of Single Amino Acid Chiral and Positional Isomers Using an Electrostatically Asymmetric Nanopore Open
Chirality is essential in nearly all biological organizations and chemical reactions but is rarely considered due to technical limitations in identifying L/D isomerization. Using OmpF, a membrane channel from Escherichia coli with an elect…
View article: Single TolC-AcrA complex formation monitored by time dependent single-channel electrophysiology
Single TolC-AcrA complex formation monitored by time dependent single-channel electrophysiology Open
Characterizing protein-protein interaction on a single molecular level is a challenge, experimentally as well as the interpretation of the data. For example, Gram-negative bacteria contain protein complexes spanning the outer and inner cel…
View article: Identification of single amino acid chiral and positional isomers using an electrostatically asymmetric nanopore
Identification of single amino acid chiral and positional isomers using an electrostatically asymmetric nanopore Open
Chirality is essential in nearly all biological organization and chemical reaction but is rarely considered due to technical limitations in identifying L/D isomerization. Using OmpF, a membrane channel from E. coli with an electrostaticall…
View article: Silicon Nitride-Based Micro-Apertures Coated with Parylene for the Investigation of Pore Proteins Fused in Free-Standing Lipid Bilayers
Silicon Nitride-Based Micro-Apertures Coated with Parylene for the Investigation of Pore Proteins Fused in Free-Standing Lipid Bilayers Open
In this work, we present a microsystem setup for performing sensitive biological membrane translocation measurements. Thin free-standing synthetic bilayer lipid membranes (BLM) were constructed in microfabricated silicon nitride apertures …
View article: A general approach to protein folding using thermostable exoshells
A general approach to protein folding using thermostable exoshells Open
In vitro protein folding is a complex process which often results in protein aggregation, low yields and low specific activity. Here we report the use of nanoscale exoshells (tES) to provide complementary nanoenvironments for the folding a…
View article: Laboratory scale production of complex protein substrates using substrate-complimentary nanoenvironments
Laboratory scale production of complex protein substrates using substrate-complimentary nanoenvironments Open
In vitro protein folding is a complex process which often results in protein aggregation, low yields and low specific activity. We report the use of nanoscale exoshells (tES) to provide specific nanoenvironments for the folding and release…
View article: Detection and quantification of small concentrations of moxifloxacin using surface‐enhanced Raman spectroscopy in a Kretschmann configuration
Detection and quantification of small concentrations of moxifloxacin using surface‐enhanced Raman spectroscopy in a Kretschmann configuration Open
A relatively simple experimental procedure is proposed for the simultaneous detection and quantitative assessment of moxifloxacin (Moxi) as an example for an antibiotic using surface‐enhanced Raman spectroscopy (SERS) performed in a Kretsc…
View article: How to Enter a Bacterium: Bacterial Porins and the Permeation of Antibiotics
How to Enter a Bacterium: Bacterial Porins and the Permeation of Antibiotics Open
Despite tremendous successes in the field of antibiotic discovery seen in the previous century, infectious diseases have remained a leading cause of death. More specifically, pathogenic Gram-negative bacteria have become a global threat du…