Michael Biddle
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View article: Atomistic TCR-ligand interactions instruct memory T-cell differentiation
Atomistic TCR-ligand interactions instruct memory T-cell differentiation Open
Summary Memory T cells endow the mammalian host with an essential form of adaptive immunity that generates durable and rapid protection following re-exposure to an infectious pathogen or a cancerous transformation 1–4 . How naive CD8⁺ T ce…
View article: Tumor-activated alloreactive T cells (TAATs) suppress solid tumors with heterogeneous tumor antigen expression 4511
Tumor-activated alloreactive T cells (TAATs) suppress solid tumors with heterogeneous tumor antigen expression 4511 Open
Description Heterogenous tumor antigen (TA) expression is one of the key hurdles in solid tumor treatment. Eliminating TA+ cells is often insufficient to control tumor growth due to the expansion of TA- tumor cells. To address this, we pro…
View article: The identification of high-performing antibodies for FUS (Uniprot ID: P35637) for use in western blot, immunoprecipitation, immunofluorescence and flow cytometry
The identification of high-performing antibodies for FUS (Uniprot ID: P35637) for use in western blot, immunoprecipitation, immunofluorescence and flow cytometry Open
RNA-binding protein Fused-in Sarcoma (FUS) plays an essential role in various cellular processes. Mutations in the C-terminal domain region, where the nuclear localization signal (NLS) is located, causes the redistribution of FUS from the …
View article: A guide to selecting high-performing antibodies for Synaptotagmin-1 (Uniprot ID P21579) for use in western blot, immunoprecipitation, immunofluorescence and flow cytometry
A guide to selecting high-performing antibodies for Synaptotagmin-1 (Uniprot ID P21579) for use in western blot, immunoprecipitation, immunofluorescence and flow cytometry Open
Synaptotagmin-1 is a synaptic vesicle transmembrane protein that senses calcium influx via its tandem C2-domains, triggering synchronous neurotransmitter release. Disruption to SYT1 is associated with neurodevelopmental disorders, highligh…
View article: BS59 The k+ channel KCa3.1 as a novel target for aortic stenosis
BS59 The k+ channel KCa3.1 as a novel target for aortic stenosis Open
Introduction & Aim Aortic stenosis (AS) is characterised by the progressive accumulation of fibrotic extracellular matrix and calcific mineral within the aortic valve (AV). These structural changes result in reduced leaflet mobility and le…
View article: A consensus platform for antibody characterization
A consensus platform for antibody characterization Open
Antibody-based research applications are critical for biological discovery. Yet, there are no industry standards to compare the performance of antibodies in various applications. We describe a knockout cell line-based antibody characteriza…
View article: Improving the integrity and reproducibility of research that uses antibodies: a technical, data sharing, behavioral and policy challenge
Improving the integrity and reproducibility of research that uses antibodies: a technical, data sharing, behavioral and policy challenge Open
Antibodies are one of the most important reagents used in biomedical and fundamental research, used to identify, and quantify proteins, contribute to knowledge of disease mechanisms, and validate drug targets. Yet many antibodies used in r…
View article: Author Response: Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications
Author Response: Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications Open
Full text Figures and data Side by side Abstract eLife assessment eLife digest Introduction Results Discussion Materials and methods Data availability References Peer review Author response Article and author information Metrics Abstract A…
View article: Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications
Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications Open
Antibodies are critical reagents to detect and characterize proteins. It is commonly understood that many commercial antibodies do not recognize their intended targets, but information on the scope of the problem remains largely anecdotal,…
View article: YCharOS open antibody characterisation data: Lessons learned and progress made
YCharOS open antibody characterisation data: Lessons learned and progress made Open
YCharOS is a collaborative initiative aimed at characterising antibodies against the entire human proteome. As of August 2023, they have presented comprehensive knockout characterisation data for 812 antibodies and 78 proteins using techni…
View article: Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications
Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications Open
Antibodies are critical reagents to detect and characterize proteins. It is commonly understood that many commercial antibodies do not recognize their intended targets, but information on the scope of the problem remains largely anecdotal,…
View article: Reviewer #2 (Public Review): Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications
Reviewer #2 (Public Review): Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications Open
Antibodies are critical reagents to detect and characterize proteins. It is commonly understood that many commercial antibodies do not recognize their intended targets, but information on the scope of the problem remains largely anecdotal,…
View article: Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications
Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications Open
Antibodies are critical reagents to detect and characterize proteins. It is commonly understood that many commercial antibodies do not recognize their intended targets, but information on the scope of the problem remains largely anecdotal,…
View article: Author Response: Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications
Author Response: Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications Open
Antibodies are critical reagents to detect and characterize proteins. It is commonly understood that many commercial antibodies do not recognize their intended targets, but information on the scope of the problem remains largely anecdotal,…
View article: Reviewer #1 (Public Review): Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications
Reviewer #1 (Public Review): Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications Open
Antibodies are critical reagents to detect and characterize proteins. It is commonly understood that many commercial antibodies do not recognize their intended targets, but information on the scope of the problem remains largely anecdotal,…
View article: Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications
Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications Open
Antibodies are critical reagents to detect and characterize proteins. It is commonly understood that many commercial antibodies do not recognize their intended targets, but information on the scope of the problem remains largely anecdotal,…
View article: Stressed out - The role of oxidative stress in airway smooth muscle dysfunction in asthma and COPD
Stressed out - The role of oxidative stress in airway smooth muscle dysfunction in asthma and COPD Open
The airway smooth muscle (ASM) surrounding the airways is dysfunctional in both asthma and chronic obstructive pulmonary disease (COPD), exhibiting; increased contraction, increased mass, increased inflammatory mediator release and decreas…
View article: Human Lung Mast Cells Impair Corticosteroid Responsiveness in Human Airway Smooth Muscle Cells
Human Lung Mast Cells Impair Corticosteroid Responsiveness in Human Airway Smooth Muscle Cells Open
The mechanisms underlying corticosteroid insensitivity in severe asthma have not been elucidated although some indirect clinical evidence points toward a role of mast cells. Here, we tested the hypothesis that mast cells can drive corticos…
View article: TGFβ1 induces resistance of human lung myofibroblasts to cell death via down‐regulation of TRPA1 channels
TGFβ1 induces resistance of human lung myofibroblasts to cell death via down‐regulation of TRPA1 channels Open
Background and Purpose TGFβ1‐mediated myofibroblast activation contributes to pathological fibrosis in many diseases including idiopathic pulmonary fibrosis (IPF), where myofibroblast resistance to oxidant‐mediated apoptosis is also eviden…
View article: Validation of antibodies for the specific detection of human TRPA1
Validation of antibodies for the specific detection of human TRPA1 Open
The transient receptor potential cation channel family member ankyrin 1 (TRPA1) is a potential target for several diseases, but detection of human TRPA1 (hTRPA1) protein in cells and tissues is problematic as rigorous antibody validation i…
View article: DP <sub>2</sub> antagonism reduces airway smooth muscle mass in asthma by decreasing eosinophilia and myofibroblast recruitment
DP <sub>2</sub> antagonism reduces airway smooth muscle mass in asthma by decreasing eosinophilia and myofibroblast recruitment Open
Cellular and computational models and bronchial biopsies from asthma patients show that a DP 2 antagonist reduces airway smooth muscle mass in asthma.
View article: Increased β2-adrenoceptor phosphorylation in airway smooth muscle in severe asthma: possible role of mast cell-derived growth factors
Increased β2-adrenoceptor phosphorylation in airway smooth muscle in severe asthma: possible role of mast cell-derived growth factors Open
Summary The purpose of this study was to investigate whether growth factors produced by activated human lung mast cells (HLMCs) impair β2-adrenoceptor (β2-AR) function in human airway smooth muscle (ASM) cells. Protein array analysis confi…