Explanipedia

Spatially ordered zygotic genome activation fulfills embryo quality control Open

Wenchao Qian, Hui Chen, H. H. Lee, Matthew C. Good · 2024

Early embryo development features autonomous, maternally-driven cell divisions that self- organize the multicellular blastula or blastocyst tissue. Maternal control cedes to the zygote starting with the onset of widespread zygotic genome a…

Assembly of hierarchical multiphase condensates using designer surfactant proteins Open

Muyang Guan, Daniel A. Hammer, Matthew C. Good · 2024

Biomolecular condensates are complex fluids formed by liquid-liquid phase separation of macromolecules. Similar to other types of soft matter, they feature a range of biophysical properties that distinguish them from the cellular milieu. A…

Delivery of Peptide Coacervates to Form Stable Interaction Hubs in Cells Open

Weiwei Tu, Rachel Theisen, Amish J. Patel, Matthew C. Good · 2024

Cells contain membrane-bound and membraneless organelles that operate as spatially distinct biochemical niches. However, these subcellular reaction centers lose fidelity with aging and as a result of disease. A grand challenge for biomedic…

Determinants that enable disordered protein assembly into discrete condensed phases Open

Rachel M. Welles, Kandarp A. Sojitra, Mikael V. Garabedian, Boao Xia, Wentao Wang , et al. · 2024

Determinants of Disordered Protein Co-Assembly Into Discrete Condensed Phases Open

Rachel M. Welles, Kandarp A. Sojitra, Mikael V. Garabedian, Boao Xia, Wentao Wang , et al. · 2023

Cells harbor numerous mesoscale membraneless compartments that house specific biochemical processes and perform distinct cellular functions. These protein and RNA-rich bodies are thought to form through multivalent interactions among prote…

Epithelial cell size dysregulation in human lung adenocarcinoma Open

Clifford W. Sandlin, Song Gu, Jun Xu, Charuhas Deshpande, Michael D. Feldman , et al. · 2022

Human cells tightly control their dimensions, but in some cancers, normal cell size control is lost. In this study we measure cell volumes of epithelial cells from human lung adenocarcinoma progression in situ. By leveraging artificial int…

Nascent transcriptome reveals orchestration of zygotic genome activation in early embryogenesis Open

Hui Chen, Matthew C. Good · 2022

Protein Condensate Formation via Controlled Multimerization of Intrinsically Disordered Sequences Open

Mikael V. Garabedian, Zhihui Su, Jorge Dabdoub, Michelle Tong, Alexander Deiters , et al. · 2022

Many proteins harboring low complexity or intrinsically disordered sequences (IDRs) are capable of undergoing liquid-liquid phase separation to form mesoscale condensates that function as biochemical niches with the ability to concentrate …

Editor's evaluation: Histone deacetylase 1 maintains lineage integrity through histone acetylome refinement during early embryogenesis Open

Matthew C. Good · 2022

Article Figures and data Abstract Editor's evaluation Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract Histone acetylation is…

Peeking under the hood of early embryogenesis: Using tools and synthetic biology to understand native control systems and sculpt tissues Open

Wenchao Qian, Matthew C. Good · 2022

Incorporation and assembly of a light-emitting enzymatic reaction into model protein condensates Open

Muyang Guan, Mikael V. Garabedian, Marcel Leutenegger, Benjamin S. Schuster, Matthew C. Good , et al. · 2022

Co-assembly of liposomes, Dendrimersomes, and Polymersomes with amphiphilic Janus dendrimers conjugated to Mono- and Tris-Nitrilotriacetic Acid (NTA, TrisNTA) enhances protein recruitment Open

Qi Xiao, Naomi Rivera-Martinez, Calvin J. Raab, Jessica G. Bermudez, Matthew C. Good , et al. · 2021

Incorporation and Assembly of a Light-Emitting Enzymatic Reaction into Model Protein Condensates Open

Muyang Guan, Mikael V. Garabedian, Marcel Leutenegger, Benjamin S. Schuster, Matthew C. Good , et al. · 2021

Eukaryotic cells partition enzymes and other cellular components into distinct subcellular compartments to generate specialized biochemical niches. A subclass of these compartments form in the absence of lipid membranes, via liquid-liquid …

Designer membraneless organelles sequester native factors for control of cell behavior Open

Mikael V. Garabedian, Wentao Wang, Jorge Dabdoub, Michelle Tong, Reese M. Caldwell , et al. · 2021

Patterning Microtubule Network Organization Reshapes Cell-Like Compartments Open

Jessica G. Bermudez, Alexander Deiters, Matthew C. Good · 2021

Eukaryotic cells contain a cytoskeletal network comprised of dynamic microtubule filaments whose spatial organization is highly plastic. Specialized microtubule architectures are optimized for different cell types and remodel with the osci…

A C. elegans Zona Pellucida domain protein functions via its ZPc domain Open

Jennifer D. Cohen, Jessica G. Bermudez, Matthew C. Good, Meera V. Sundaram · 2020

Zona Pellucida domain (ZP) proteins are critical components of the body’s external-most protective layers, apical extracellular matrices (aECMs). Although their loss or dysfunction is associated with many diseases, it remains unclear how Z…

Integrating cellular dimensions with cell differentiation during early development Open

Hui Chen, Wenchao Qian, Matthew C. Good · 2020

Nuclear sizER in Early Development Open

Hui Chen, Matthew C. Good · 2020

A<i>C. elegans</i>Zona Pellucida domain protein functions via its ZPc domain Open

Jennifer D. Cohen, Jessica G. Bermudez, Matthew C. Good, Meera V. Sundaram · 2020

Zona Pellucida domain (ZP) proteins are critical components of the body’s external-most protective layers, apical extracellular matrices (aECMs). Although their loss or dysfunction is associated with many diseases, it remains unclear how Z…

Identifying sequence perturbations to an intrinsically disordered protein that determine its phase-separation behavior Open

Benjamin S. Schuster, Gregory L. Dignon, Wai Shing Tang, Fleurie M. Kelley, Aishwarya Kanchi Ranganath , et al. · 2020

Significance Membraneless organelles are assemblies of highly concentrated biomolecules that form through liquid–liquid phase separation. These assemblies are often enriched in intrinsically disordered proteins (IDPs), which play an import…

SPLIT: Stable Protein Coacervation Using a Light Induced Transition Open

Ellen H. Reed, Benjamin S. Schuster, Matthew C. Good, Daniel A. Hammer · 2020

Protein coacervates serve as hubs to concentrate and sequester proteins and nucleotides and thus function as membraneless organelles to manipulate cell physiology. We have engineered a coacervating protein to create tunable, synthetic memb…

Identifying Sequence Perturbations to an Intrinsically Disordered Protein that Determine Its Phase Separation Behavior Open

Benjamin S. Schuster, Gregory L. Dignon, Wai Shing Tang, Fleurie M. Kelley, Aishwarya Kanchi Ranganath , et al. · 2020

Phase separation of intrinsically disordered proteins (IDPs) commonly underlies the formation of membraneless organelles, which compartmentalize molecules intracellularly in the absence of a lipid membrane. Identifying the protein sequence…

Imaging nascent transcription in wholemount vertebrate embryos to characterize zygotic genome activation Open

Hui Chen, Matthew C. Good · 2020

Encapsulation of hydrophobic components in dendrimersomes and decoration of their surface with proteins and nucleic acids Open

Paola Della Torre, Qi Xiao, Irene Buzzacchera, Samuel E. Sherman, Khosrow Rahimi , et al. · 2019

Significance Lipid vesicles are globular assemblies that compartmentalize, encapsulate, transport, and provide signal transmission and communication between cells. In living systems, these vesicles perform critical functions to sustain lif…

Spatiotemporal Patterning of Zygotic Genome Activation in a Model Vertebrate Embryo Open

Hui Chen, Lily C. Einstein, Shawn C. Little, Matthew C. Good · 2019

Encoding biological recognition in a bicomponent cell-membrane mimic Open

César Rodriguez‐Emmenegger, Qi Xiao, Nina Yu. Kostina, Samuel E. Sherman, Khosrow Rahimi , et al. · 2019

Significance The seminal fluid mosaic model of the cell membranes suggests a lipid bilayer sea, in which cholesterol, proteins, glycoconjugates, and other components are swimming. Complementing this view, a microsegregated rafts model pred…

Sequence Determinants of Protein Phase Separation of the Intrinsically Disordered RGG Domain from LAF-1 Open

Benjamin S. Schuster, Gregory L. Dignon, Craig N. Jahnke, Matthew C. Good, Daniel A. Hammer , et al. · 2019

Cell parts to complex processes, from the bottom up Open

Matthew C. Good, Xavier Trepat · 2018

Controllable protein phase separation and modular recruitment to form responsive membraneless organelles Open

Benjamin S. Schuster, Ellen H. Reed, Ranganath Parthasarathy, Craig N. Jahnke, Reese M. Caldwell , et al. · 2018

Optochemical Control of Protein Localization and Activity within Cell-like Compartments Open

Reese M. Caldwell, Jessica G. Bermudez, David Thai, Chanat Aonbangkhen, Benjamin S. Schuster , et al. · 2018

We report inducible dimerization strategies for controlling protein positioning, enzymatic activity, and organelle assembly inside synthetic cell-like compartments upon photostimulation. Using a photocaged TMP-Haloligand compound, we demon…

Matthew C. Good YOU? Author Swipe