Matthew E. Bechard
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View article: Transcriptomic profiling reveals the role of Hedgehog signaling as a biomarker and in the pathogenesis of Ménétrier's disease
Transcriptomic profiling reveals the role of Hedgehog signaling as a biomarker and in the pathogenesis of Ménétrier's disease Open
Both Ménétrier's disease (MD) and juvenile polyposis syndrome (JPS) are rare premalignant conditions that can lead to gastric cancer. MD is an acquired disease without known causative mutations that is characterized by increased expression…
View article: Unbiased recording of clonal potency reveals species-specific regulation of mammalian intestine
Unbiased recording of clonal potency reveals species-specific regulation of mammalian intestine Open
SUMMARY The mammalian intestine regenerates rapidly after damage, yet the clonal dynamics and species-specific regulation of different populations remain poorly understood. Here we used synthetic or naturally occurring DNA alterations to r…
View article: Dipeptidase-1–knockout mice develop invasive tumors with features of microsatellite-unstable colorectal cancer
Dipeptidase-1–knockout mice develop invasive tumors with features of microsatellite-unstable colorectal cancer Open
Dipeptidase-1 (DPEP1) is highly upregulated in colorectal cancer (CRC), with its enzymatic function linked to invasion and metastasis. More recently, DPEP1 was found to serve as a receptor for neutrophils when expressed by activated endoth…
View article: Lrig3 restricts the size of the colon stem cell compartment
Lrig3 restricts the size of the colon stem cell compartment Open
The cellular census of the colonic crypt is tightly regulated, yet the molecular mechanisms that regulate this census are not fully understood. Lrig3, a transmembrane protein, is expressed in colonic crypt epithelial cells, including the s…
View article: Pancreatic cancers suppress negative feedback of glucose transport to reprogram chromatin for metastasis
Pancreatic cancers suppress negative feedback of glucose transport to reprogram chromatin for metastasis Open
Although metastasis is the most common cause of cancer deaths, metastasis-intrinsic dependencies remain largely uncharacterized. We previously reported that metastatic pancreatic cancers were dependent on the glucose-metabolizing enzyme ph…
View article: Purification, kinetic characterization, and site-directed mutagenesis of <em>Methanothermobacter thermautotrophicus</em> RFAP Synthase Produced in <em>Escherichia coli</em>
Purification, kinetic characterization, and site-directed mutagenesis of <em>Methanothermobacter thermautotrophicus</em> RFAP Synthase Produced in <em>Escherichia coli</em> Open
Methane-producing archaea are among a select group of microorganisms that utilize tetrahydromethanopterin (H4MPT) as a one-carbon carrier instead of tetrahydrofolate. In H4MPT biosynthesis, β-ribofuranosylaminobenzene 5'-phosphate (RFAP) s…
View article: ROCK-nmMyoII, Notch and <i>Neurog3</i> gene-dosage link epithelial morphogenesis with cell fate in the pancreatic endocrine-progenitor niche
ROCK-nmMyoII, Notch and <i>Neurog3</i> gene-dosage link epithelial morphogenesis with cell fate in the pancreatic endocrine-progenitor niche Open
During mouse pancreas organogenesis, endocrine cells are born from progenitors residing in an epithelial plexus niche. After a period in a lineage-primed Neurog3LO state, progenitors become endocrine committed via upregulation of Neurog3. …
View article: FUCCI tracking shows cell‐cycle‐dependent <i>Neurog3</i> variation in pancreatic progenitors
FUCCI tracking shows cell‐cycle‐dependent <i>Neurog3</i> variation in pancreatic progenitors Open
During pancreas organogenesis, Neurog3 HI endocrine‐committing cells are generated from a population of Sox9 + mitotic progenitors with only a low level of Neurog3 transcriptional activity ( Neurog3 TA.LO ). Low‐level Neurog3 protein, in N…
View article: FUCCI tracking shows that Neurog3 levels vary with cell-cycle phase in endocrine-biased pancreatic progenitors
FUCCI tracking shows that Neurog3 levels vary with cell-cycle phase in endocrine-biased pancreatic progenitors Open
Neurog3 HI endocrine-committing cells are generated from a population of Sox9 + mitotic progenitors with only a low level of Neurog3 transcriptional activity ( Neurog3 TA.LO ). Low-level Neurog3 protein, in Neurog3 TA.LO cells, is required…
View article: New ideas connecting the cell cycle and pancreatic endocrine-lineage specification
New ideas connecting the cell cycle and pancreatic endocrine-lineage specification Open
Neurogenin 3 (Neurog3) is a pro-endocrine transcription factor required for endocrine-lineage specification during mouse pancreas development. It was a long-standing notion that Neurog3 activation ...
View article: Precommitment low-level <i>Neurog3</i> expression defines a long-lived mitotic endocrine-biased progenitor pool that drives production of endocrine-committed cells
Precommitment low-level <i>Neurog3</i> expression defines a long-lived mitotic endocrine-biased progenitor pool that drives production of endocrine-committed cells Open
The current model for endocrine cell specification in the pancreas invokes high-level production of the transcription factor Neurogenin 3 (Neurog3) in Sox9 + bipotent epithelial cells as the trigger for endocrine commitment, cell cycle exi…