Matthew J. Tuttle
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View article: Prophage induction states drive structural and synergistic outcomes in marine bacterial biofilms
Prophage induction states drive structural and synergistic outcomes in marine bacterial biofilms Open
Microbes commonly live in biofilms, dense spatially structured assemblages where temperate phages can influence interactions amongst closely related strains. Yet, how these phage-host interactions impact biofilm architecture and ecological…
View article: Shelf-Stable <i>Sporosarcina pasteurii</i> Formulation for Scalable Laboratory and Field-Based Production of Biocement
Shelf-Stable <i>Sporosarcina pasteurii</i> Formulation for Scalable Laboratory and Field-Based Production of Biocement Open
Biocement is an environmentally friendly alternative to traditional cement that is produced via microbially induced calcium carbonate precipitation (MICP) and has great potential to mitigate the environmental harms of cement and concrete u…
View article: Complete genome sequence of <i>Sporosarcina pasteurii</i> type strain DSM33
Complete genome sequence of <i>Sporosarcina pasteurii</i> type strain DSM33 Open
Here, we present the complete genome sequence of Sporosarcina pasteurii type strain DSM33, a model organism for microbially induced calcium carbonate precipitation. This genome consists of a single 3.3-Mb chromosome and is an improvement u…
View article: Correction: Microbiomes and Planctomycete diversity in large-scale aquaria habitats
Correction: Microbiomes and Planctomycete diversity in large-scale aquaria habitats Open
[This corrects the article DOI: 10.1371/journal.pone.0267881.].
View article: Phenol-chloroform DNA extraction from Sporosarcina pasteurii v1
Phenol-chloroform DNA extraction from Sporosarcina pasteurii v1 Open
This protocol is for extraction of genomic DNA from Sporosarcina pasteurii. It is based on a standard phenol-chloroform DNA extraction method.
View article: Microbially Induced Calcium Carbonate Precipitation by <i>Sporosarcina pasteurii</i> : a Case Study in Optimizing Biological CaCO <sub>3</sub> Precipitation
Microbially Induced Calcium Carbonate Precipitation by <i>Sporosarcina pasteurii</i> : a Case Study in Optimizing Biological CaCO <sub>3</sub> Precipitation Open
Current production of traditional concrete requires enormous energy investment that accounts for approximately 5 to 8% of the world’s annual CO 2 production. Biocement is a building material that is already in industrial use and has the po…
View article: Promoting Science Literacy and Awareness across the Globe: the Role of Scientists as Science Ambassadors
Promoting Science Literacy and Awareness across the Globe: the Role of Scientists as Science Ambassadors Open
Science literacy has many personal and societal benefits that allows for better informed decision-making. Although the importance of science literacy is recognized globally, there are many challenges associated with its promotion.
View article: Microbiomes and Planctomycete diversity in large-scale aquaria habitats
Microbiomes and Planctomycete diversity in large-scale aquaria habitats Open
In commercial large-scale aquaria, controlling levels of nitrogenous compounds is essential for macrofauna health. Naturally occurring bacteria are capable of transforming toxic nitrogen species into their more benign counterparts and play…
View article: Plasmid-Mediated Stabilization of Prophages
Plasmid-Mediated Stabilization of Prophages Open
The consequences of prophage induction on the physiology of microbial populations are varied and include enhanced biofilm formation, conferral of virulence, and increased opportunity for horizontal gene transfer. These traits lead to compe…
View article: Lysogeny in the oceans: Lessons from cultivated model systems and a reanalysis of its prevalence
Lysogeny in the oceans: Lessons from cultivated model systems and a reanalysis of its prevalence Open
Summary In the oceans, viruses that infect bacteria (phages) influence a variety of microbially mediated processes that drive global biogeochemical cycles. The nature of their influence is dependent upon infection mode, be it lytic or lyso…
View article: Genetically similar temperate phages form coalitions with their shared host that lead to niche-specific fitness effects
Genetically similar temperate phages form coalitions with their shared host that lead to niche-specific fitness effects Open
Temperate phages engage in long-term associations with their hosts that may lead to mutually beneficial interactions, of which the full extent is presently unknown. Here, we describe an environmentally relevant model system with a single h…