Matthew S. Macauley
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View article: An ultrasensitive and modular platform to detect Siglec ligands and control immune cell function
An ultrasensitive and modular platform to detect Siglec ligands and control immune cell function Open
Siglecs are immunomodulatory receptors that regulate immune cell function. A fundamental challenge in studying Siglec-ligand interactions is the low affinity of Siglecs for their ligands. Inspired by how nature uses multivalency, we develo…
View article: Non-Carbohydrate Inhibitors of Sialic Acid-binding Immunomodulatory-type Lectin-7 (Siglec-7) Discovered from Genetically Encoded Bicyclic Peptide Libraries
Non-Carbohydrate Inhibitors of Sialic Acid-binding Immunomodulatory-type Lectin-7 (Siglec-7) Discovered from Genetically Encoded Bicyclic Peptide Libraries Open
Glycan-binding proteins (GBP) are among the most difficult to drug targets. This deficiency delays clinical progress for therapeutically important GBPs. We employed bicyclic genetically encoded libraries (BiGELs), produced by chemical modi…
View article: Adjuvants and MHCII modulate the immunogenicity of subdominant epitopes in Plasmodium vivax Duffy binding protein
Adjuvants and MHCII modulate the immunogenicity of subdominant epitopes in Plasmodium vivax Duffy binding protein Open
Antibody responses to protein antigens are driven by epitope hierarchies that promote the immunogenicity of select epitopes. For antigenically variable pathogens, immune responses toward conserved epitopes are critical to elicit strain-tra…
View article: Fundamental motifs and parity within the crystallographic point groups
Fundamental motifs and parity within the crystallographic point groups Open
This paper analyzes the Hasse diagram, or family tree, of the 3D crystal classes, also called geometric crystal classes. The 32 point-group classes are partitioned into seven crystal systems. In this paper, the structures of these systems …
View article: Presentation of immunoregulatory sialoglycans on T cells is divergent between mice and humans
Presentation of immunoregulatory sialoglycans on T cells is divergent between mice and humans Open
Glycans are emerging as important regulators of T cell function but remain poorly characterized across the functionally distinct populations that exist in vivo. Here, we couple single-cell analysis technologies with soluble lectins and che…
View article: CD16 and Siglec expression refine the phenotypic heterogeneity of steady-state myeloid-derived suppressor cells
CD16 and Siglec expression refine the phenotypic heterogeneity of steady-state myeloid-derived suppressor cells Open
Background Polymorphonuclear myeloid-derived suppressor cells (PMN-MDSCs) mediate cancer immune suppression by promoting an immunosuppressive microenvironment that inhibits effective anti-tumor immunity. However, they are still a poorly un…
View article: Glycolipid recognition and binding by Siglec-6 hinges on interactions with the cell membrane
Glycolipid recognition and binding by Siglec-6 hinges on interactions with the cell membrane Open
Sialic acid-binding immunoglobulin-type lectins (Siglecs) regulate immune response through interactions with sialylated glycan epitopes on glycoproteins and glycolipids. Human Siglecs count 14 unique proteins allocated to two subsets; grou…
View article: Tumor glyco-immunology, glyco-immune checkpoints and immunotherapy
Tumor glyco-immunology, glyco-immune checkpoints and immunotherapy Open
Immunotherapy, including immune checkpoint inhibition, has transformed the prognosis of many patients with cancer. However, most patients have primary or secondary resistant tumors to currently available cancer immunotherapies. Changes in …
View article: CD33 Epitope Editing Unlocks UM171-Expanded Cord Blood Grafts for AML Immunotherapy
CD33 Epitope Editing Unlocks UM171-Expanded Cord Blood Grafts for AML Immunotherapy Open
Immunotherapies in acute myeloid leukemia (AML) are limited by shared antigen expression between leukemic and healthy hematopoietic cells, leading to on-target toxicity. Here we developed a clinically scalable strategy to engineer cord blo…
View article: Carbohydrate sulfation as a critical modulator of siglec-sialoglycan interactions
Carbohydrate sulfation as a critical modulator of siglec-sialoglycan interactions Open
Siglecs are sialic acid-binding immunomodulatory receptors that regulate immune homeostasis. Abnormal alterations in sialic acid-containing glycans (sialoglycans) on tissues or cells are key drivers of various diseases, including cancer, n…
View article: Targeting Inhibitory Siglec-3 to Suppress IgE-Mediated Human Basophil Degranulation
Targeting Inhibitory Siglec-3 to Suppress IgE-Mediated Human Basophil Degranulation Open
View article: Exploiting CD22 on antigen-specific B cells to prevent allergy to the major peanut allergen Ara h 2
Exploiting CD22 on antigen-specific B cells to prevent allergy to the major peanut allergen Ara h 2 Open
View article: Synthesis of 4-azido sialic acid for testing against Siglec-7 and in metabolic oligosaccharide engineering
Synthesis of 4-azido sialic acid for testing against Siglec-7 and in metabolic oligosaccharide engineering Open
Peracetylated 4AzNeu5Ac is incorporated well into cell glycoconjugates. Deprotection via a thioglycoside facilitated chemoenzymatic synthesis of a C4-azido sialoside, which was studied for binding to Siglec-7 using native mass spectrometry.
View article: Roles for Siglec-glycan interactions in regulating immune cells
Roles for Siglec-glycan interactions in regulating immune cells Open
Cell surface complex carbohydrates, known as glycans, are positioned to be the first point of contact between two cells. Indeed, interactions between glycans with glycan-binding can modulate cell-cell interactions. This concept is particul…
View article: 579 Siglec-7/9 receptors suppress T cell immunity via trogocytosis and represent degradable targets for cancer immunotherapy
579 Siglec-7/9 receptors suppress T cell immunity via trogocytosis and represent degradable targets for cancer immunotherapy Open
View article: Enzyme-Sialylation-Controlled Chemical Sulfation of Glycan Epitopes for Decoding the Binding of Siglec Ligands
Enzyme-Sialylation-Controlled Chemical Sulfation of Glycan Epitopes for Decoding the Binding of Siglec Ligands Open
Widely distributed in nature, sulfated glycan epitopes play important roles in diverse pathophysiological processes. However, due to their structural complexity, the preparation of glycan epitopes with structurally defined sulfation patter…
View article: Reshaping the tumor microenvironment by degrading glycoimmune checkpoints Siglec-7 and -9
Reshaping the tumor microenvironment by degrading glycoimmune checkpoints Siglec-7 and -9 Open
Cancer treatment has been rapidly transformed by the development of immune checkpoint inhibitors targeting CTLA-4 and PD-1/PD-L1. However, many patients fail to respond, especially those with an immunosuppressive tumor microenvironment (TM…
View article: A Unified Atlas of T cell Glycophysiology
A Unified Atlas of T cell Glycophysiology Open
SUMMARY Glycans are emerging as important regulators of T cell function but remain poorly characterized across the functionally distinct populations that exist in vivo . Here, we couple single-cell analysis technologies with soluble lectin…
View article: Pre-treatment with high molecular weight free PEG effectively suppresses anti-PEG antibody induction by PEG-liposomes in mice
Pre-treatment with high molecular weight free PEG effectively suppresses anti-PEG antibody induction by PEG-liposomes in mice Open
View article: A kinetic trapping approach for facile access to 3FaxNeu5Ac and a photo-crosslinkable sialyltransferase probe
A kinetic trapping approach for facile access to 3FaxNeu5Ac and a photo-crosslinkable sialyltransferase probe Open
Sialic acid (Neu5Ac) is installed onto glycoconjugates by sialyltransferases (STs) using cytidine monophosphate-Neu5Ac (CMP-B-D-Neu5Ac) as their donor. The only class of cell-active ST inhibitors are those based on a 3FaxNeu5Ac scaffold, w…
View article: Chemoenzymatic Synthesis of Sulfated <i>N</i>-Glycans Recognized by Siglecs and Other Glycan-Binding Proteins
Chemoenzymatic Synthesis of Sulfated <i>N</i>-Glycans Recognized by Siglecs and Other Glycan-Binding Proteins Open
Sulfated N-glycans are present in many glycoproteins, which are implicated in playing important roles in biological recognition processes. Here, we report the systematic chemoenzymatic synthesis of a library of sulfated and sialylat…
View article: Human CD22 Inhibits Murine B Cell Receptor Activation in a Human CD22 Transgenic Mouse Model
Human CD22 Inhibits Murine B Cell Receptor Activation in a Human CD22 Transgenic Mouse Model Open
CD22, a sialic acid-binding Ig-type lectin (Siglec) family member, is an inhibitory coreceptor of the BCR with established roles in health and disease. The restricted expression pattern of CD22 on B cells and most B cell lymphomas has made…
View article: Dissecting the abilities of murine Siglecs to interact with gangliosides
Dissecting the abilities of murine Siglecs to interact with gangliosides Open
View article: Alzheimer’s disease associated isoforms of human CD33 distinctively modulate microglial cell responses in 5XFAD mice
Alzheimer’s disease associated isoforms of human CD33 distinctively modulate microglial cell responses in 5XFAD mice Open
View article: Sialic acid blockade inhibits the metastatic spread of prostate cancer to bone
Sialic acid blockade inhibits the metastatic spread of prostate cancer to bone Open
View article: Advances in understanding and exploiting Siglec–glycan interactions
Advances in understanding and exploiting Siglec–glycan interactions Open
Sialic-acid-binding immunoglobulin-type lectins (Siglecs) are a family of cell-surface immunomodulatory receptors that recognize sialic-acid-containing glycans. The majority of Siglecs have an inhibitory motif in their intercellular domain…
View article: ST3 beta-galactoside alpha-2,3-sialyltransferase 1 (ST3Gal1) synthesis of Siglec ligands mediates anti-tumour immunity in prostate cancer
ST3 beta-galactoside alpha-2,3-sialyltransferase 1 (ST3Gal1) synthesis of Siglec ligands mediates anti-tumour immunity in prostate cancer Open
Immune checkpoint blockade has yet to produce robust anti-cancer responses for prostate cancer. Sialyltransferases have been shown across several solid tumours, including breast, melanoma, colorectal and prostate to promote immune suppress…
View article: Molecular Insights into O-Linked Sialoglycans Recognition by the Siglec-Like SLBR-N (SLBR<sub>UB10712</sub>) of <i>Streptococcus gordonii</i>
Molecular Insights into O-Linked Sialoglycans Recognition by the Siglec-Like SLBR-N (SLBR<sub>UB10712</sub>) of <i>Streptococcus gordonii</i> Open
Streptococcus gordonii is a Gram-positive bacterial species that typically colonizes the human oral cavity, but can also cause local or systemic diseases. Serine-rich repeat (SRR) glycoproteins exposed on the S. gordonii bact…
View article: Dissecting the Ability of Siglecs To Antagonize Fcγ Receptors
Dissecting the Ability of Siglecs To Antagonize Fcγ Receptors Open
Fcγ receptors (FcγRs) play key roles in the effector function of IgG, but their inappropriate activation plays a role in several disease etiologies. Therefore, it is critical to better understand how FcγRs are regulated. Numerous studies s…
View article: A Unified Atlas of T Cell Glycophysiology
A Unified Atlas of T Cell Glycophysiology Open