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View article: Supplementary Figure S6 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer
Supplementary Figure S6 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer Open
EV injected into mice are imported by cells in PDAC tumors.
View article: Supplementary Data 3 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer
Supplementary Data 3 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer Open
Supplementary Data 3. HPaMECs treated with WT vs. HuR KO EVs
View article: Supplementary Table S4 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer
Supplementary Table S4 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer Open
Flow cytometry gating strategy for orthotopic pancreatic tumors
View article: Supplementary Figure S4 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer
Supplementary Figure S4 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer Open
Additional mouse model staining.
View article: Supplementary Figure S3 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer
Supplementary Figure S3 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer Open
Repeating in vitro EV treatments with an additional endothelial cell line.
View article: Figure 5 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer
Figure 5 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer Open
Tumor-intrinsic HuR directly alters endothelial cell function via EV signaling. A, Schematic of the PalmGRET reporter labeling the inner leaflet of all membranes with GFP-nLuc via integration of a palmitoylation sequence. The constr…
View article: Figure 4 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer
Figure 4 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer Open
Tumor-intrinsic HuR promotes endothelial cell recruitment and sprouting in PDAC tumors. A, Immunoblot of KPC WT and HuR-KO clones 6, 8, and 9 probed for HuR and loading control vinculin. B, KPC WT and HuR-KO cells were implan…
View article: Supplementary Figure S5 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer
Supplementary Figure S5 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer Open
Validation of PalmGRET reporter in KPC cells.
View article: Supplementary Figure S2 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer
Supplementary Figure S2 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer Open
Validation of EV import imaging in vitro.
View article: Figure 1 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer
Figure 1 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer Open
HuR affects PDAC EV cargoes relating to endothelial cell functions. A, Schematic of EV isolation from PANC-1 WT vs. HuR-KO cells for EV validation, RNA-seq, and proteomics. B, Immunoblot of cell lysates (CL) and SEC fractions…
View article: Supplementary Table S3 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer
Supplementary Table S3 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer Open
Flow cytometry antibody information
View article: Supplementary Table S2 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer
Supplementary Table S2 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer Open
Immunofluorescence antibody information
View article: Supplementary Data 2 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer
Supplementary Data 2 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer Open
Supplementary Data 2. Proteomic data from WT vs. HuR KO EV cargo
View article: Figure 3 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer
Figure 3 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer Open
WT EVs directly alter endothelial cell transcriptome, migration, barrier function, and tube formation. A, HPaMECs were treated with WT vs. HuR-KO EVs for 24 hours before RNA-seq and phenotypic assessment of migration, monolayer perm…
View article: Supplementary Data 1 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer
Supplementary Data 1 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer Open
Supplementary Data 1. WT vs. HuR KO EV cargo
View article: Supplementary Figure S1 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer
Supplementary Figure S1 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer Open
PANC-1 HuR KO and EV isolation and phospho-proteomics.
View article: Data from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer
Data from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer Open
Pancreatic ductal adenocarcinoma (PDAC) tumors are hypovascular with collapsed and dysfunctional vessels that limit immunosurveillance and contribute to early metastatic events. However, current antiangiogenic therapies have failed in PDAC…
View article: Figure 2 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer
Figure 2 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer Open
Endothelial cell abundance correlates with ELAVL1 expression, and endothelial cells import PDAC EVs. A, Correlation between ELAVL1 expression in PDAC cells and the presence of endothelial cells in publicly available pa…
View article: Supplementary Table S1 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer
Supplementary Table S1 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer Open
Immunoblotting antibody information
View article: Figure 6 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer
Figure 6 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer Open
HuR WT EVs are tumor promoting and directly decrease ICAM-1 expression on endothelial cells. A, Schematic of EV isolation from KPC WT and HuR-KO cells via SEC. B, Immunoblot of cell lysates (CL) and SEC fractions 1, 7–10 (EV …
View article: HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer
HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer Open
Pancreatic ductal adenocarcinoma (PDAC) tumors are hypovascular with collapsed and dysfunctional vessels that limit immunosurveillance and contribute to early metastatic events. However, current antiangiogenic therapies have failed in PDAC…
View article: PIN1 Drives Cellular Plasticity and Immune Modulation in Chronic Pancreatitis
PIN1 Drives Cellular Plasticity and Immune Modulation in Chronic Pancreatitis Open
Background and Aims Chronic pancreatitis (CP) is characterized by inflammation, fibrosis, and acinar-to-ductal metaplasia (ADM). PIN1, known to drive oncogenic signaling and cellular plasticity in cancer, has an unexplored role in CP. This…
View article: Pancreatic cancer-intrinsic HuR regulates the pro-tumorigenic properties of extracellular vesicles
Pancreatic cancer-intrinsic HuR regulates the pro-tumorigenic properties of extracellular vesicles Open
Pancreatic ductal adenocarcinoma (PDAC) tumors contain chaotic vasculature that limits immune surveillance and promotes early events in the metastatic cascade. However, current antiangiogenic therapies have failed in PDAC, and thus, it rem…
View article: Analysis of queuosine and 2-thio tRNA modifications by high throughput sequencing
Analysis of queuosine and 2-thio tRNA modifications by high throughput sequencing Open
Queuosine (Q) is a conserved tRNA modification at the wobble anticodon position of tRNAs that read the codons of amino acids Tyr, His, Asn, and Asp. Q-modification in tRNA plays important roles in the regulation of translation efficiency a…
View article: Kinetic modeling reveals additional regulation at co-transcriptional level by post-transcriptional sRNA regulators
Kinetic modeling reveals additional regulation at co-transcriptional level by post-transcriptional sRNA regulators Open
Small RNAs (sRNAs) are important gene regulators in bacteria. Many sRNAs act post-transcriptionally by affecting translation and degradation of the target mRNAs upon base-pairing interactions. Here we present a general approach combining i…
View article: Dynamic interactions between the RNA chaperone Hfq, small regulatory RNAs, and mRNAs in live bacterial cells
Dynamic interactions between the RNA chaperone Hfq, small regulatory RNAs, and mRNAs in live bacterial cells Open
RNA-binding proteins play myriad roles in regulating RNAs and RNA-mediated functions. In bacteria, the RNA chaperone Hfq is an important post-transcriptional gene regulator. Using live-cell super-resolution imaging, we can distinguish Hfq …
View article: Author response: Dynamic interactions between the RNA chaperone Hfq, small regulatory RNAs, and mRNAs in live bacterial cells
Author response: Dynamic interactions between the RNA chaperone Hfq, small regulatory RNAs, and mRNAs in live bacterial cells Open
Article Figures and data Abstract eLife digest Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract RNA-binding proteins play myr…
View article: Kinetic model of small RNA-mediated regulation suggests that a small RNA can regulate co-transcriptionally
Kinetic model of small RNA-mediated regulation suggests that a small RNA can regulate co-transcriptionally Open
All code and data used in the manuscript "Kinetic model of small RNA-mediated regulation suggests that a small RNA can regulate co-transcriptionally"
View article: Kinetic model of small RNA-mediated regulation suggests that a small RNA can regulate co-transcriptionally
Kinetic model of small RNA-mediated regulation suggests that a small RNA can regulate co-transcriptionally Open
Small RNAs (sRNAs) are important regulators of gene expression in bacteria, particularly during stress responses. Many genetically and biochemically well characterized sRNAs regulate gene expression post-transcriptionally, by affecting tra…
View article: Dynamic interactions between the RNA chaperone Hfq, small regulatory RNAs and mRNAs in live bacterial cells
Dynamic interactions between the RNA chaperone Hfq, small regulatory RNAs and mRNAs in live bacterial cells Open
RNA binding proteins play myriad roles in controlling and regulating RNAs and RNA-mediated functions, often through simultaneous binding to other cellular factors. In bacteria, the RNA chaperone Hfq modulates post-transcriptional gene regu…