Matthew W. Fields
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View article: Integration of localized microbiome, metabolome, and clinical datasets predicts healing in chronic wounds among veterans
Integration of localized microbiome, metabolome, and clinical datasets predicts healing in chronic wounds among veterans Open
The chronic wound microenvironment consists of a complex milieu of host cells, microbial species, and metabolites. While much is known about wound microbiomes, our knowledge of metabolic landscapes influencing wound healing is limited. Fur…
View article: Sediment and groundwater metagenomes from subsurface microbial communities from the Oak Ridge National Laboratory Oak Ridge Reservation, Oak Ridge, Tennessee, USA
Sediment and groundwater metagenomes from subsurface microbial communities from the Oak Ridge National Laboratory Oak Ridge Reservation, Oak Ridge, Tennessee, USA Open
We report 26 subsurface sediment and 9 groundwater metagenomes from the Oak Ridge Reservation at Oak Ridge, TN, USA. Samples were collected from various depths and phases (attached vs planktonic) to study subsurface microbial metabolism, t…
View article: Modest functional diversity decline and pronounced composition shifts of microbial communities in a mixed waste-contaminated aquifer
Modest functional diversity decline and pronounced composition shifts of microbial communities in a mixed waste-contaminated aquifer Open
View article: pH-dependent genotypic and phenotypic variability in <i>Oleidesulfovibrio alaskensis</i> G20
pH-dependent genotypic and phenotypic variability in <i>Oleidesulfovibrio alaskensis</i> G20 Open
Sulfate-reducing bacteria (SRB) exhibit versatile metabolic adaptability with significant flexibility influenced by pH fluctuations, which play a critical role in biogeochemical cycles. In this study, we used a model SRB, Oleidesulfovibrio…
View article: Effect of surface roughness on the microbiologically influenced corrosion (MIC) of copper 101
Effect of surface roughness on the microbiologically influenced corrosion (MIC) of copper 101 Open
The effect of varying surface roughness on microbiologically influenced corrosion by a model sulfate reducing bacterium Oleidesulfovibrio alaskensis G20 culture on copper 101 coupons was investigated using microscopic, spectroscopic and su…
View article: Unlocking the societal potential of engineered living materials
Unlocking the societal potential of engineered living materials Open
View article: Influence of Copper on Oleidesulfovibrio alaskensis G20 Biofilm Formation
Influence of Copper on Oleidesulfovibrio alaskensis G20 Biofilm Formation Open
Copper is known to have toxic effects on bacterial growth. This study aimed to determine the influence of copper ions on Oleidesulfovibrio alaskensis G20 biofilm formation in a lactate-C medium supplemented with variable copper ion concent…
View article: Global challenges and microbial biofilms: Identification of priority questions in biofilm research, innovation and policy
Global challenges and microbial biofilms: Identification of priority questions in biofilm research, innovation and policy Open
View article: Project Assessment for Biological and Environmental Research: Report from the BER Advisory Committee
Project Assessment for Biological and Environmental Research: Report from the BER Advisory Committee Open
The construction, operation, and stewardship of large-scale scientific user facilities and cutting edge capabilities have been integral to the mission of the U.S. Department of Energy (DOE) Office of Science from its earliest days. To help…
View article: Contribution of Microorganisms with the Clade II Nitrous Oxide Reductase to Suppression of Surface Emissions of Nitrous Oxide
Contribution of Microorganisms with the Clade II Nitrous Oxide Reductase to Suppression of Surface Emissions of Nitrous Oxide Open
The sources and sinks of nitrous oxide, as control emissions to the atmosphere, are generally poorly constrained for most environmental systems. Initial depth-resolved analysis of nitrous oxide flux from observation wells and the proximal …
View article: Table of Contents
Table of Contents Open
View article: Sediment and Groundwater Metagenomes from Subsurface Microbial Communities from the Oak Ridge National Laboratory Field Research Center, Oak Ridge, TN, USA
Sediment and Groundwater Metagenomes from Subsurface Microbial Communities from the Oak Ridge National Laboratory Field Research Center, Oak Ridge, TN, USA Open
We report 26 subsurface sediment and 9 groundwater metagenomes from the Oak Ridge Reservation at Oak Ridge, TN, USA. Samples were collected from various depths and phases (attached vs planktonic) to study subsurface microbial metabolism, t…
View article: A comprehensive strategy for stable, high productivity cultivation of microalgae with controllable biomass composition
A comprehensive strategy for stable, high productivity cultivation of microalgae with controllable biomass composition Open
This project was performed primarily as a partnership between three Universities – The University of Toledo (UToledo), Montana State University (MSU) and The University of North Carolina at Chapel Hill (UNC). North Carolina State Universit…
View article: Environmental stress mediates groundwater microbial community assembly
Environmental stress mediates groundwater microbial community assembly Open
View article: Understanding phycosomal dynamics to improve industrial microalgae cultivation
Understanding phycosomal dynamics to improve industrial microalgae cultivation Open
View article: Molecular mechanisms and environmental adaptations of flagellar loss and biofilm growth of <i>Rhodanobacter</i> under environmental stress
Molecular mechanisms and environmental adaptations of flagellar loss and biofilm growth of <i>Rhodanobacter</i> under environmental stress Open
Biofilms aid bacterial adhesion to surfaces via direct and indirect mechanisms, and formation of biofilms is considered as an important strategy for adaptation and survival in suboptimal environmental conditions. However, the molecular und…
View article: Investigating In Situ Methanogenic Activity in Antrim Shale via Deuterium Injection
Investigating In Situ Methanogenic Activity in Antrim Shale via Deuterium Injection Open
View article: Mixed waste contamination selects for a mobile genetic element population enriched in multiple heavy metal resistance genes
Mixed waste contamination selects for a mobile genetic element population enriched in multiple heavy metal resistance genes Open
Mobile genetic elements (MGEs) like plasmids, viruses, and transposable elements can provide fitness benefits to their hosts for survival in the presence of environmental stressors. Heavy metal resistance genes (HMRGs) are frequently obser…
View article: Phycosome Dynamics During Successive Outdoor Microalgae Cultivation from Late Summer to Fall
Phycosome Dynamics During Successive Outdoor Microalgae Cultivation from Late Summer to Fall Open
View article: Mixed Waste Contamination Selects for a Mobile Genetic Element Population Enriched in Multiple Heavy Metal Resistance Genes
Mixed Waste Contamination Selects for a Mobile Genetic Element Population Enriched in Multiple Heavy Metal Resistance Genes Open
Mobile genetic elements (MGEs) like plasmids, viruses, and transposable elements can provide fitness benefits to their hosts for survival in the presence of environmental stressors. Heavy metal resistance genes (HMRGs) are frequently obser…
View article: Biomass distribution and activity of respective subsurface sediments and groundwater within a shallow subsurface ecosystem
Biomass distribution and activity of respective subsurface sediments and groundwater within a shallow subsurface ecosystem Open
Subsurface environments represent diverse microbial communities responsible for mediating biogeochemical cycles linked to the turnover of organic and inorganic carbon important to groundwater used by human society for consumption, irrigati…
View article: Algal amendment enhances biogenic methane production from coals of different thermal maturity
Algal amendment enhances biogenic methane production from coals of different thermal maturity Open
The addition of small amounts of algal biomass to stimulate methane production in coal seams is a promising low carbon renewable coalbed methane enhancement technique. However, little is known about how the addition of algal biomass amendm…
View article: Pressurized upflow reactor system for the bioconversion of coal to methane: Investigation of the coal/sand interface effect
Pressurized upflow reactor system for the bioconversion of coal to methane: Investigation of the coal/sand interface effect Open
Microbial generation of coal bed methane (CBM) represents a significant source of natural gas on Earth. While biostimulation has been demonstrated in batch cultures, environmental parameters such as overburden pressure and formation water …
View article: Ecosystems and Networks Integrated with Genes and Molecular Assemblies (Final Scientific/Technical Report)
Ecosystems and Networks Integrated with Genes and Molecular Assemblies (Final Scientific/Technical Report) Open
The ENIGMA science focus area (SFA) is a multi-disciplinary, multi-institutional research effort focused on addressing foundational knowledge gaps in environmental microbial communities by studying groundwater and sediment microbiomes in t…
View article: A global perspective on bacterial diversity in the terrestrial deep subsurface
A global perspective on bacterial diversity in the terrestrial deep subsurface Open
While recent efforts to catalogue Earth’s microbial diversity have focused upon surface and marine habitats, 12–20 % of Earth’s biomass is suggested to exist in the terrestrial deep subsurface, compared to ~1.8 % in the deep subseafloor. M…
View article: KBase Narrative - Supplemental Data for "Mixed waste contamination selects for a mobile genetic element population enriched in multiple heavy metal resistance genes"
KBase Narrative - Supplemental Data for "Mixed waste contamination selects for a mobile genetic element population enriched in multiple heavy metal resistance genes" Open
Here we provide the complete set of circular elements produced by SCAPP from metagenomic data that may represent mobile genetic elements (MGEs). For the publication, these sequences were de-replicated within the high [U] and low [U] sample…
View article: Subsurface hydrocarbon degradation strategies in low- and high-sulfate coal seam communities identified with activity-based metagenomics
Subsurface hydrocarbon degradation strategies in low- and high-sulfate coal seam communities identified with activity-based metagenomics Open
View article: In Situ Enhancement and Isotopic Labeling of Biogenic Coalbed Methane
In Situ Enhancement and Isotopic Labeling of Biogenic Coalbed Methane Open
Subsurface microbial (biogenic) methane production is an important part of the global carbon cycle that has resulted in natural gas accumulations in many coal beds worldwide. Laboratory studies suggest that complex carbon-containing nutrie…
View article: Genomic Features and Pervasive Negative Selection in <i>Rhodanobacter</i> Strains Isolated from Nitrate and Heavy Metal Contaminated Aquifer
Genomic Features and Pervasive Negative Selection in <i>Rhodanobacter</i> Strains Isolated from Nitrate and Heavy Metal Contaminated Aquifer Open
Despite the dominance of Rhodanobacter species in the subsurface of the contaminated Oak Ridge Reservation (ORR) site, very little is known about the mechanisms underlying their adaptions to the various stressors present at ORR. Recently, …
View article: Enhancement of Acetoclastic Methanogenesis during <i>In Situ</i> Biostimulation of Coalbed Methane Generation
Enhancement of Acetoclastic Methanogenesis during <i>In Situ</i> Biostimulation of Coalbed Methane Generation Open