Matthias Höhne
YOU?
Author Swipe
View article: Ribosomal Incorporation of Thioxanthone as a Noncanonical Amino Acid Facilitates the Engineering of Photoenzymes
Ribosomal Incorporation of Thioxanthone as a Noncanonical Amino Acid Facilitates the Engineering of Photoenzymes Open
Photocatalysis in biocatalytic systems provides a promising approach for achieving selective and efficient chemical transformations under mild conditions. Naturally occurring photoactive cofactors are rare. To overcome this limitation, gen…
View article: Chemoenzymatic Route toward a De Novo Enantioselective Total Synthesis of (<i>S</i>)‐Baclofen Based on Metal‐Catalyzed Hydroformylation and Enzymatic Transamination
Chemoenzymatic Route toward a De Novo Enantioselective Total Synthesis of (<i>S</i>)‐Baclofen Based on Metal‐Catalyzed Hydroformylation and Enzymatic Transamination Open
This study explores the chemoenzymatic synthesis of ( S )‐baclofen, which involves a sequential combination of transition metal catalysis and biocatalysis. The synthesis approach starts from a readily accessible cinnamic acid ester that is…
View article: Combining Photochemical Oxyfunctionalization and Enzymatic Catalysis for the Synthesis of Chiral Pyrrolidines and Azepanes
Combining Photochemical Oxyfunctionalization and Enzymatic Catalysis for the Synthesis of Chiral Pyrrolidines and Azepanes Open
Chiral heterocyclic alcohols and amines are frequently used building blocks in the synthesis of fine chemicals and pharmaceuticals. Herein, we report a one-pot photoenzymatic synthesis route for N-Boc-3-amino/hydroxy-pyrrolidine and…
View article: Biocatalytic cascade combining an engineered pyranose 2-oxidase and transaminases for the synthesis of amino sugars
Biocatalytic cascade combining an engineered pyranose 2-oxidase and transaminases for the synthesis of amino sugars Open
C2 chiral amino sugars are important synthons in various fields. By combining reactions of a pyranose 2-oxidase and transaminases, the catalytic cascades for synthesizing rare amino sugars mannosamine, glucosamine, and galactosamine were a…
View article: Co‐Immobilization of a Multi‐Enzyme Cascade: (<i>S</i>)‐Selective Amine Transaminases, <scp>l</scp>‐Amino Acid Oxidase and Catalase
Co‐Immobilization of a Multi‐Enzyme Cascade: (<i>S</i>)‐Selective Amine Transaminases, <span>l</span>‐Amino Acid Oxidase and Catalase Open
An enzyme cascade was established previously consisting of a recycling system with an l ‐amino acid oxidase (hcLAAO4) and a catalase (hCAT) for different α‐keto acid co‐substrates of ( S )‐selective amine transaminases (ATAs) in kinetic re…
View article: Biosynthesis of Furfurylamines in Batch and Continuous Flow by Immobilized Amine Transaminases
Biosynthesis of Furfurylamines in Batch and Continuous Flow by Immobilized Amine Transaminases Open
Building blocks with amine functionality are crucial in the chemical industry. Biocatalytic syntheses and chemicals derived from renewable resources are increasingly desired to achieve sustainable production of these amines. As a result, r…
View article: Biosynthesis of Furfurylamines in Batch and Continuous Flow by Immobilized Amine Transaminases
Biosynthesis of Furfurylamines in Batch and Continuous Flow by Immobilized Amine Transaminases Open
Building blocks with amine functionality are crucial in the chemical industry. Biocatalytic syntheses and chemicals derived from renewable resources are increasingly desired to achieve sustainable production of these amines. As a result, r…
View article: Biosynthesis of Furfurylamines in Batch and Continuous Flow by Immobilized Amine Transaminases
Biosynthesis of Furfurylamines in Batch and Continuous Flow by Immobilized Amine Transaminases Open
Building blocks with amine functionality are crucial in the chemical industry. Biocatalytic syntheses and chemicals derived from renewable resources are increasingly desired to achieve sustainable production of these amines. As a result, r…
View article: Marine <i>Bacteroidetes</i> enzymatically digest xylans from terrestrial plants
Marine <i>Bacteroidetes</i> enzymatically digest xylans from terrestrial plants Open
Marine Bacteroidetes that degrade polysaccharides contribute to carbon cycling in the ocean. Organic matter, including glycans from terrestrial plants, might enter the oceans through rivers. Whether marine bacteria degrade structurally rel…
View article: Comparison of Four Immobilization Methods for Different Transaminases
Comparison of Four Immobilization Methods for Different Transaminases Open
Biocatalytic syntheses often require unfavorable conditions, which can adversely affect enzyme stability. Consequently, improving the stability of biocatalysts is needed, and this is often achieved by immobilization. In this study, we aime…
View article: Shifting the pH Optima of (R)-Selective Transaminases by Protein Engineering
Shifting the pH Optima of (R)-Selective Transaminases by Protein Engineering Open
Amine transaminases (ATAs) are powerful biocatalysts for the stereoselective synthesis of chiral amines. However, wild-type ATAs usually show pH optima at slightly alkaline values and exhibit low catalytic activity under physiological cond…
View article: A growth selection system for the directed evolution of amine-forming or converting enzymes
A growth selection system for the directed evolution of amine-forming or converting enzymes Open
View article: Activity Levels of Amine Transaminases Correlate with Active Site Hydrophobicity
Activity Levels of Amine Transaminases Correlate with Active Site Hydrophobicity Open
Amine transaminases (ATAs) are biocatalysts for the synthesis of chiral amines and can be identified in sequence databases by specific sequence motifs. This study shows that the activity level towards the model substrate 1‐phenylethylamine…
View article: Characterization of proteins from the 3N5M family reveals an operationally stable amine transaminase
Characterization of proteins from the 3N5M family reveals an operationally stable amine transaminase Open
View article: Recombinant <scp>l</scp>‐Amino Acid Oxidase with Broad Substrate Spectrum for Co‐substrate Recycling in (<i>S</i>)‐Selective Transaminase‐Catalyzed Kinetic Resolutions
Recombinant <span>l</span>‐Amino Acid Oxidase with Broad Substrate Spectrum for Co‐substrate Recycling in (<i>S</i>)‐Selective Transaminase‐Catalyzed Kinetic Resolutions Open
Chiral and enantiopure amines can be produced by enantioselective transaminases via kinetic resolution of amine racemates. This transamination reaction requires stoichiometric amounts of co‐substrate. A dual‐enzyme recycling system overcom…
View article: Hybrid catalysis:towards an optimal combination of catalysts Application to HMF valorization
Hybrid catalysis:towards an optimal combination of catalysts Application to HMF valorization Open
National audience
View article: Efficient Site‐Selective Immobilization of Aldehyde‐Tagged Peptides and Proteins by Knoevenagel Ligation
Efficient Site‐Selective Immobilization of Aldehyde‐Tagged Peptides and Proteins by Knoevenagel Ligation Open
The aldehyde tag is appropriate to selectively label proteins, prepare antibody‐drug conjugates or to immobilize enzymes or antibodies for biotechnological and medical applications. The cysteine within the consensus sequence CxPxR of the a…
View article: Recent trends in biocatalysis
Recent trends in biocatalysis Open
Technological developments enable the discovery of novel enzymes, the advancement of enzyme cascade designs and pathway engineering, moving biocatalysis into an era of technology integration, intelligent manufacturing and enzymatic total s…
View article: Creation of (<i>R</i>)-Amine Transaminase Activity within an α-Amino Acid Transaminase Scaffold
Creation of (<i>R</i>)-Amine Transaminase Activity within an α-Amino Acid Transaminase Scaffold Open
The enzymatic transamination of ketones into (R)-amines represents an important route for accessing a range of pharmaceuticals or building blocks. Although many publications have dealt with enzyme discovery, protein engineering, and…
View article: Next to Bauhaus
Next to Bauhaus Open
Der vorliegende Band ist die zweite Ausgabe einer Dokumentation der Lehre und Forschung an der Fakultät für Architektur, Facility Management und Geoinformation sowie ein Rückblick auf 20 erfolgreiche Jahre kooperativen Lehrens und Lernens …
View article: Chemoenzymatic Synthesis of Sertraline
Chemoenzymatic Synthesis of Sertraline Open
A chemoenzymatic approach has been developed for the preparation of sertraline, an established anti‐depressant drug. Ketoreductases (KREDs) were employed to yield a key chiral precursor. The bioreduction of the racemic tetralone exhibited …
View article: Three‐liquid‐phase Spinning Reactor for the Transaminase‐catalyzed Synthesis and Recovery of a Chiral Amine
Three‐liquid‐phase Spinning Reactor for the Transaminase‐catalyzed Synthesis and Recovery of a Chiral Amine Open
A device for the transaminase‐catalysed synthesis combined with continuous recovery of chiral amines was designed. The system enabled the separation of the reaction components in three liquid phases: a reaction phase, an organic solvent ph…
View article: One‐pot Synthesis of 4‐Aminocyclohexanol Isomers by Combining a Keto Reductase and an Amine Transaminase
One‐pot Synthesis of 4‐Aminocyclohexanol Isomers by Combining a Keto Reductase and an Amine Transaminase Open
The efficient multifunctionalization by one‐pot or cascade catalytic systems has developed as an important research field, but is often challenging due to incompatibilities or cross‐reactivities of the catalysts leading to side product for…
View article: Random Mutagenesis‐Driven Improvement of Carboxylate Reductase Activity using an Amino Benzamidoxime‐Mediated High‐Throughput Assay
Random Mutagenesis‐Driven Improvement of Carboxylate Reductase Activity using an Amino Benzamidoxime‐Mediated High‐Throughput Assay Open
Carboxylic acid reductases (CARs) catalyze the direct adenosine triphosphate (ATP) and nicotinamide adenine dinucleotide phosphate (NADPH) dependent reduction of carboxylic acids to their corresponding aldehydes. The identification and imp…
View article: Combining Photo‐Organo Redox‐ and Enzyme Catalysis Facilitates Asymmetric C‐H Bond Functionalization
Combining Photo‐Organo Redox‐ and Enzyme Catalysis Facilitates Asymmetric C‐H Bond Functionalization Open
In this study, we combined photo‐organo redox catalysis and biocatalysis to achieve asymmetric C–H bond functionalization of simple alkane starting materials. The photo‐organo catalyst anthraquinone sulfate (SAS) was employed to oxyfunctio…
View article: Molecular recognition of the beta‐glucans laminarin and pustulan by a SusD‐like glycan‐binding protein of a marine <i>Bacteroidetes</i>
Molecular recognition of the beta‐glucans laminarin and pustulan by a SusD‐like glycan‐binding protein of a marine <i>Bacteroidetes</i> Open
Marine bacteria catabolize carbohydrate polymers of algae, which synthesize these structurally diverse molecules in ocean surface waters. Although algal glycans are an abundant carbon and energy source in the ocean, the molecular details t…
View article: HRB Neuwürschnitz – Lösungsansätze für den Zielkonflikt zwischen EU-WRRL und Hochwasserschutz
HRB Neuwürschnitz – Lösungsansätze für den Zielkonflikt zwischen EU-WRRL und Hochwasserschutz Open
View article: Alteration of the Donor/Acceptor Spectrum of the (S)-Amine Transaminase from Vibrio fluvialis
Alteration of the Donor/Acceptor Spectrum of the (S)-Amine Transaminase from Vibrio fluvialis Open
To alter the amine donor/acceptor spectrum of an (S)-selective amine transaminase (ATA), a library based on the Vibrio fluvialis ATA targeting four residues close to the active site (L56, W57, R415 and L417) was created. A 3DM-derived alig…