Matthias Kästner
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View article: A novel approach for calorespirometry: Integrating a CO2 sensor into an isothermal microcalorimeter for simultaneous measurement of microbial heat evolution and mineralization
A novel approach for calorespirometry: Integrating a CO2 sensor into an isothermal microcalorimeter for simultaneous measurement of microbial heat evolution and mineralization Open
View article: Coral-dwelling Caribbean gobies exhibit distinct skin microbiota compared to other sympatric species
Coral-dwelling Caribbean gobies exhibit distinct skin microbiota compared to other sympatric species Open
Microbial communities fundamentally shape ecosystem function and biodiversity across all biological systems through complex dynamics. In coral reef ecosystems, understanding the dynamics of these microbial communities has become critical f…
View article: Expression and Immunogenicity Analysis of Recombinant <i>Leptospira Interrogans</i> Surface Protein LigA in Mouse Model
Expression and Immunogenicity Analysis of Recombinant <i>Leptospira Interrogans</i> Surface Protein LigA in Mouse Model Open
Background Pathogenic strains of spirochetes of Leptospira spp. cause a globally distributed zoonotic disease called leptospirosis. The disease has several clinical manifestations, ranging from asymptomatic and subclinical infection to fat…
View article: Decoupling of heat and CO2 release during decomposition of cellulose and its building blocks in soil
Decoupling of heat and CO2 release during decomposition of cellulose and its building blocks in soil Open
View article: An Isothermal Microcalorimeter with Integrated CO2 Sensor for Simultaneous Measurement of Microbial Heat Evolution and Mineralization
An Isothermal Microcalorimeter with Integrated CO2 Sensor for Simultaneous Measurement of Microbial Heat Evolution and Mineralization Open
Soil, as the largest terrestrial carbon sink, plays a crucial role in carbon sequestration. Within soil systems, microorganisms decompose soil organic matter to generate energy and obtain carbon for growth, concomitantly release heat and C…
View article: Experimental access to cellulose oxidation and the dynamics of microbial carbon and energy use in artificial soil under varying temperature, water content, and C/N ratio
Experimental access to cellulose oxidation and the dynamics of microbial carbon and energy use in artificial soil under varying temperature, water content, and C/N ratio Open
View article: Non-extractable residues (NER) in persistence assessment: effect on the degradation half-life of chemicals
Non-extractable residues (NER) in persistence assessment: effect on the degradation half-life of chemicals Open
The ECHA guidance on persistence (P) assessment has been updated with respect to non-extractable residues (NER). Unless further characterized, total NER shall be considered as non-degraded parent compound. We investigated how different NER…
View article: Non-extractable Residues (Ner) in Persistence Assessment – Effect on the Degradation Half-life of Chemicals
Non-extractable Residues (Ner) in Persistence Assessment – Effect on the Degradation Half-life of Chemicals Open
The ECHA guidance on persistence (P) assessment has been updated with respect to non-extractable residues (NER). Unless further characterized, total NER shall be considered as non-degraded parent compound. We investigated how different NER…
View article: Assessing energy fluxes and carbon use in soil as controlled by microbial activity - A thermodynamic perspective A perspective paper
Assessing energy fluxes and carbon use in soil as controlled by microbial activity - A thermodynamic perspective A perspective paper Open
Soil organic matter (SOM) plays a central role for both the C cycle and soil functions. Plants provide the input and heterotrophic (micro)organisms are essential for the turnover. Microbial metabolism links matter and energy fluxes and gen…
View article: Linking mass balances and thermodynamic energy balances at different water contents, temperature and nutrient supply in simplified model systems with artificial soils
Linking mass balances and thermodynamic energy balances at different water contents, temperature and nutrient supply in simplified model systems with artificial soils Open
Microbial communities in soil play a pivotal role in nutrient cycling and organic matter decomposition, relying on carbon (C) and energy sources for growth. The allocation of these substrates, crucial for their metabolic activities, is inf…
View article: Thermodynamic control of microbial turnover of organic substrates in soils
Thermodynamic control of microbial turnover of organic substrates in soils Open
Microbial turnover of organic substrates is a key process in soil organic matter formation and turnover. As microorganisms require both carbon and energy for growth and maintenance, carbon and energy fluxes in soils are tightly coupled. On…
View article: Expanding Understanding: Investigating the Information Value of Calorespirometric Ratio in Dynamic Processes of Soil Microbial Growth Using Calorimetry
Expanding Understanding: Investigating the Information Value of Calorespirometric Ratio in Dynamic Processes of Soil Microbial Growth Using Calorimetry Open
The preservation of soil services and functions is sustained by the catalytic activity of microbial communities. These communities use energy and carbon – especially for microbial growth – from the transformation of organic mat…
View article: Enhancing insights: exploring the information content of calorespirometric ratio in dynamic soil microbial growth processes through calorimetry
Enhancing insights: exploring the information content of calorespirometric ratio in dynamic soil microbial growth processes through calorimetry Open
Catalytic activity of microbial communities maintains the services and functions of soils. Microbial communities require energy and carbon for microbial growth, which they obtain by transforming organic matter (OM), oxidizing a fraction of…
View article: P3D-BRNS v1.0.0: a three-dimensional, multiphase, multicomponent, pore-scale reactive transport modelling package for simulating biogeochemical processes in subsurface environments
P3D-BRNS v1.0.0: a three-dimensional, multiphase, multicomponent, pore-scale reactive transport modelling package for simulating biogeochemical processes in subsurface environments Open
The porous microenvironment of soil offers various environmental functions which are governed by physical and reactive processes. Understanding reactive transport processes in porous media is essential for many natural systems (soils, aqui…
View article: Gibbs energy or enthalpy—What is relevant for microbial C‐turnover in soils?
Gibbs energy or enthalpy—What is relevant for microbial C‐turnover in soils? Open
International audience
View article: scMAR-Seq: a novel workflow for targeted single-cell genomics of microorganisms using radioactive labeling
scMAR-Seq: a novel workflow for targeted single-cell genomics of microorganisms using radioactive labeling Open
Current methods for the identification of specific microorganisms based on an in situ metabolism are often hampered by insufficient sensitivity and habitat complexity. Here, we present a novel approach for identifying and sequencing single…
View article: Hypertonic stress induced changes of Pseudomonas fluorescens adhesion towards soil minerals studied by AFM
Hypertonic stress induced changes of Pseudomonas fluorescens adhesion towards soil minerals studied by AFM Open
View article: Bacterial cell-mineral associations and their stability under varying moisture conditions
Bacterial cell-mineral associations and their stability under varying moisture conditions Open
Soil particle wettability controls the water dynamics of dry and unsaturated soil and has an impact on many processes where water is involved. Pure soil minerals are usually wettable, but under environmental conditions they are easily cove…
View article: SoilSystems, a research program on systems ecology of soils – energy discharge modulated by microbiome and boundary conditions
SoilSystems, a research program on systems ecology of soils – energy discharge modulated by microbiome and boundary conditions Open
Large fluxes of solar energy conserved in organic matter pass through soil as conduit from primary production to mineralisation. Soil organisms are channelling the flux, are fuelled by the energy, and contribute by their bio- and necromass…
View article: Combining results on organic pollutant degradation and on soil organic matter turnover gives indications for the control of key microbial transformation processes in soils under global change
Combining results on organic pollutant degradation and on soil organic matter turnover gives indications for the control of key microbial transformation processes in soils under global change Open
Microbial transformation of organic compounds is a key process in soil. Microorganisms are responsible for important soil functions such as degradation of organic pollutants, but also for the formation and transformation of soil organic ma…
View article: Linking mass balances and thermodynamic energy balances in simplified model systems with artificial soils
Linking mass balances and thermodynamic energy balances in simplified model systems with artificial soils Open
Soils represent the largest terrestrial carbon (C) sink and understanding its dynamics is crucial. The metabolic degradation and stabilization of soil organic matter (SOM) follow the rules of thermodynamics. In the catabolic reaction, SOM …
View article: Silylation: a reproducible method for characterization of non-extractable residues (NER) of organic chemicals in the assessment of persistence
Silylation: a reproducible method for characterization of non-extractable residues (NER) of organic chemicals in the assessment of persistence Open
NER, a hidden hazard or a safe sink–we are going to find that out!
View article: Changes in cell surface properties of <i>Pseudomonas fluorescens</i> by adaptation to NaCl induced hypertonic stress
Changes in cell surface properties of <i>Pseudomonas fluorescens</i> by adaptation to NaCl induced hypertonic stress Open
Determination of the effect of water stress on the surface properties of bacteria is crucial to study bacterial induced soil water repellency. Changes in the environmental conditions may affect several properties of bacteria such as the ce…
View article: Comment on gmd-2022-86
Comment on gmd-2022-86 Open
The porous microenvironment of soil offers various environmental functions which are governed by physical and reactive processes. Understanding reactive transport processes in porous media is essential for many natural systems (soils, aqui…
View article: Water Stress-Driven Changes in Bacterial Cell Surface Properties
Water Stress-Driven Changes in Bacterial Cell Surface Properties Open
Changes in precipitation frequency, intensity, and temporal distribution are projected to result in increased frequency and intensity of droughts and heavy rainfall events. Prolonged droughts can promote the development of soil water repel…
View article: Microcosm test for pesticide fate assessment in planted water filters: 13C,15N-labeled glyphosate as an example
Microcosm test for pesticide fate assessment in planted water filters: 13C,15N-labeled glyphosate as an example Open
View article: Supplementary material to "P3D-BRNS v1.0.0: A Three-dimensional, Multiphase, Multicomponent, Pore-scale Reactive Transport Modelling Package for Simulating Biogeochemical Processes in Subsurface Environments"
Supplementary material to "P3D-BRNS v1.0.0: A Three-dimensional, Multiphase, Multicomponent, Pore-scale Reactive Transport Modelling Package for Simulating Biogeochemical Processes in Subsurface Environments" Open
Derivation of the global indicator function Section S.2: Analytical solution for mass transfer across fluid-fluid interface Section S.3: Results obtained with P3D-BRNS from denitrification scenario that are not included in the main man…
View article: P3D-BRNS v1.0.0: A Three-dimensional, Multiphase, Multicomponent, Pore-scale Reactive Transport Modelling Package for Simulating Biogeochemical Processes in Subsurface Environments
P3D-BRNS v1.0.0: A Three-dimensional, Multiphase, Multicomponent, Pore-scale Reactive Transport Modelling Package for Simulating Biogeochemical Processes in Subsurface Environments Open
The porous microenvironment of soil offers various environmental functions which are governed by physical and reactive processes. Understanding reactive transport processes in porous media is essential for many natural systems (soils, aqui…
View article: Critical evaluation of the microbial turnover to biomass approach for the estimation of biogenic non-extractable residues (NER)
Critical evaluation of the microbial turnover to biomass approach for the estimation of biogenic non-extractable residues (NER) Open
Background Persistence is a key criterion for the risk assessment of chemicals. In degradation tests, microbial biodegradation of labeled test chemicals leads to the incorporation of the label in microbial biomass, resulting in biogenic no…
View article: Serial Measurements of Refractive Index, Glucose and Protein to Assess Gastric Liquid Nutrient Transport—A Proof-of-Principal Study
Serial Measurements of Refractive Index, Glucose and Protein to Assess Gastric Liquid Nutrient Transport—A Proof-of-Principal Study Open
Delayed gastric emptying contributes to complications as aspiration or malnutrition. Among patients suffering from acute neurological diseases, motility disorders are prevalent but poorly understood. Thus, methods to measure gastric emptyi…