Matthias Wojtynek
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View article: Mutation-induced filaments of folded proteins are inert and non-toxic in a cellular system
Mutation-induced filaments of folded proteins are inert and non-toxic in a cellular system Open
Filamentous protein assemblies are essential for cellular functions but can also form aberrantly through mutations that induce self-interactions between folded protein subunits. These assemblies, which we refer to as agglomerates, differ f…
View article: A conserved mechanism of membrane fusion in nuclear pore complex assembly
A conserved mechanism of membrane fusion in nuclear pore complex assembly Open
The nuclear pore complex (NPC) forms a large channel that spans the double lipid bilayer of the nuclear envelope and is the central gateway for macromolecular transport between the nucleus and cytoplasm in eukaryotes. NPC biogenesis requir…
View article: Discovery of New Binders for DCAF1, an Emerging Ligase Target in the Targeted Protein Degradation Field
Discovery of New Binders for DCAF1, an Emerging Ligase Target in the Targeted Protein Degradation Field Open
In this study, we describe the rapid identification of potent binders for the WD40 repeat domain (WDR) of DCAF1. This was achieved by two rounds of iterative focused screening of a small set of compounds selected on the basis of internal W…
View article: Structural investigation of eukaryotic cells: From the periphery to the interior by cryo-electron tomography
Structural investigation of eukaryotic cells: From the periphery to the interior by cryo-electron tomography Open
Cryo-electron tomography (cryo-ET) combines a close-to-life preservation of the cell with high-resolution three-dimensional (3D) imaging. This allows to study the molecular architecture of the cellular landscape and provides unprecedented …
View article: An amphipathic helix in Brl1 is required for nuclear pore complex biogenesis in S. cerevisiae
An amphipathic helix in Brl1 is required for nuclear pore complex biogenesis in S. cerevisiae Open
The nuclear pore complex (NPC) is the central portal for macromolecular exchange between the nucleus and cytoplasm. In all eukaryotes, NPCs assemble into an intact nuclear envelope (NE) during interphase, but the process of NPC biogenesis …
View article: Author response: An amphipathic helix in Brl1 is required for nuclear pore complex biogenesis in S. cerevisiae
Author response: An amphipathic helix in Brl1 is required for nuclear pore complex biogenesis in S. cerevisiae Open
View article: The Nuclear Pore Complex: Birth, Life, and Death of a Cellular Behemoth
The Nuclear Pore Complex: Birth, Life, and Death of a Cellular Behemoth Open
Nuclear pore complexes (NPCs) are the only transport channels that cross the nuclear envelope. Constructed from ~500–1000 nucleoporin proteins each, they are among the largest macromolecular assemblies in eukaryotic cells. Thanks to advanc…
View article: An amphipathic helix in Brl1 is required for membrane fusion during nuclear pore complex biogenesis in <i>S. cerevisiae</i>
An amphipathic helix in Brl1 is required for membrane fusion during nuclear pore complex biogenesis in <i>S. cerevisiae</i> Open
The nuclear pore complex (NPC) is the central portal for macromolecular exchange between the nucleus and cytoplasm. In all eukaryotes, NPCs assemble into an intact nuclear envelope (NE) during interphase, but the process of NPC biogenesis …
View article: The cellular environment shapes the nuclear pore complex architecture
The cellular environment shapes the nuclear pore complex architecture Open
View article: Maturation Kinetics of a Multiprotein Complex Revealed by Metabolic Labeling
Maturation Kinetics of a Multiprotein Complex Revealed by Metabolic Labeling Open
View article: Code for Maturation kinetics of a multiprotein complex revealed by metabolic labeling
Code for Maturation kinetics of a multiprotein complex revealed by metabolic labeling Open
A project for fitting a (binary) assembly tree to dynamic labeling data from AP-MS proteomics. This code was used in the work published as "Maturation kinetics of a multiprotein complex revealed by metabolic labeling" in Onischenko et al. …
View article: Maturation Kinetics of a Multiprotein Complex Revealed by Metabolic Labeling
Maturation Kinetics of a Multiprotein Complex Revealed by Metabolic Labeling Open
View article: Mapping the native organization of the yeast nuclear pore complex using nuclear radial intensity measurements
Mapping the native organization of the yeast nuclear pore complex using nuclear radial intensity measurements Open
Significance The nuclear pore complex (NPC) is a huge protein assembly that selectively transports macromolecules across the nuclear envelope (NE) of eukaryotes. Determining the precise organization of that machinery has been a long-standi…
View article: Cellular and Structural Studies of Eukaryotic Cells by Cryo-Electron Tomography
Cellular and Structural Studies of Eukaryotic Cells by Cryo-Electron Tomography Open
The architecture of protein assemblies and their remodeling during physiological processes is fundamental to cells. Therefore, providing high-resolution snapshots of macromolecular complexes in their native environment is of major importan…
View article: Rpn11-mediated ubiquitin processing in an ancestral archaeal ubiquitination system
Rpn11-mediated ubiquitin processing in an ancestral archaeal ubiquitination system Open