Maxim E. Darvin
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View article: Optical Coherence Elastography—A Novel Non-Invasive Optical Method for Real-Time Determination of Substances Penetration and Associated Skin Dehydration
Optical Coherence Elastography—A Novel Non-Invasive Optical Method for Real-Time Determination of Substances Penetration and Associated Skin Dehydration Open
Non-invasive, in vivo assessment of target substances penetration into the skin remains a significant challenge in dermatology and cosmetology. While various optical methods have been employed for this purpose, each has inherent limitation…
View article: Non-Invasive Spectroscopic Determination of the Skin and Blood Carotenoids of Term and Preterm Infants in the First Month of Life and the Influence of Free Radical-Mediated Diseases
Non-Invasive Spectroscopic Determination of the Skin and Blood Carotenoids of Term and Preterm Infants in the First Month of Life and the Influence of Free Radical-Mediated Diseases Open
Postpartum adaptation causes an increased formation of free radicals (FRs) in the organism, which can lead to development of various FR-mediated diseases (FRMDs) in the newborn. The present study investigates the kinetics of skin and blood…
View article: Seeing through the skin: Optical methods for visualizing transdermal drug delivery with microneedles
Seeing through the skin: Optical methods for visualizing transdermal drug delivery with microneedles Open
Optical methods play a pivotal role in advancing transdermal drug delivery research, particularly with the emergence of microneedle technology. This review presents a comprehensive analysis of optical methods used in studying transdermal d…
View article: Dual Role of Stratum Corneum Carotenes/Lycopene Against the Development of Chemotherapy-induced PPE in Patients With Cancer
Dual Role of Stratum Corneum Carotenes/Lycopene Against the Development of Chemotherapy-induced PPE in Patients With Cancer Open
Palmar-plantar erythrodysaesthesia (PPE) is a common side effect of chemotherapy treatment in patients with cancer. The exact pathophysiologic mechanisms of the development of PPE remain unclear. Here, we report two important physiological…
View article: Topical Peroxisome Proliferator-Activated Receptor Agonist Induces Molecular Alterations Enhancing Barrier Function and Water-Holding Capacity of the Human Stratum Corneum In Vivo
Topical Peroxisome Proliferator-Activated Receptor Agonist Induces Molecular Alterations Enhancing Barrier Function and Water-Holding Capacity of the Human Stratum Corneum In Vivo Open
The peroxisome proliferator-activated receptor (PPAR) ligands modulate a variety of skin functions but are rarely used in cosmetics. The aim of this double-blind, placebo-controlled, in vivo study was to determine the effect of a topically…
View article: Two-Photon-Excited FLIM of NAD(P)H and FAD—Metabolic Activity of Fibroblasts for the Diagnostics of Osteoimplant Survival
Two-Photon-Excited FLIM of NAD(P)H and FAD—Metabolic Activity of Fibroblasts for the Diagnostics of Osteoimplant Survival Open
Bioinert materials such as the zirconium dioxide and aluminum oxide are widely used in surgery and dentistry due to the absence of cytotoxicity of the materials in relation to the surrounding cells of the body. However, little attention ha…
View article: Characterization and Efficacy of Essential Oil-Based Cosmetic Formulations for Acne-Prone Skin
Characterization and Efficacy of Essential Oil-Based Cosmetic Formulations for Acne-Prone Skin Open
This randomized, placebo-controlled, double-blind clinical in vivo study aimed to evaluate the clinical efficacy of a cosmetic formulation for non-inflammatory acne using essential oils. Fifty-three male participants were divided into four…
View article: Atopic Dermatitis: Molecular Alterations between Lesional and Non-Lesional Skin Determined Noninvasively by In Vivo Confocal Raman Microspectroscopy
Atopic Dermatitis: Molecular Alterations between Lesional and Non-Lesional Skin Determined Noninvasively by In Vivo Confocal Raman Microspectroscopy Open
Atopic dermatitis (AD)/atopic eczema is a chronic relapsing inflammatory skin disease affecting nearly 14% of the adult population. An important pathogenetic pillar in AD is the disrupted skin barrier function (SBF). The atopic stratum cor…
View article: Optical Methods for Non-Invasive Determination of Skin Penetration: Current Trends, Advances, Possibilities, Prospects, and Translation into In Vivo Human Studies
Optical Methods for Non-Invasive Determination of Skin Penetration: Current Trends, Advances, Possibilities, Prospects, and Translation into In Vivo Human Studies Open
Information on the penetration depth, pathways, metabolization, storage of vehicles, active pharmaceutical ingredients (APIs), and functional cosmetic ingredients (FCIs) of topically applied formulations or contaminants (substances) in ski…
View article: Quantification of skin penetration of caffeine and propylene glycol applied topically in a mixture by tailored multivariate curve resolution‐alternating least squares of depth‐resolved Raman spectra
Quantification of skin penetration of caffeine and propylene glycol applied topically in a mixture by tailored multivariate curve resolution‐alternating least squares of depth‐resolved Raman spectra Open
The quantitative determination of topically applied substances in the skin is severely limited and represents a challenging task. The porcine skin ex vivo was topically treated with a gel containing caffeine (CF) and propylene glycol (PG),…
View article: Altered structure indicating reduced barrier function of lesional compared to non‐lesional psoriatic skin—A non‐invasive in vivo study of the human stratum corneum with confocal Raman micro‐spectroscopy
Altered structure indicating reduced barrier function of lesional compared to non‐lesional psoriatic skin—A non‐invasive in vivo study of the human stratum corneum with confocal Raman micro‐spectroscopy Open
Psoriasis, one of the most common skin diseases affecting roughly 2%–3% of the world population, is associated with a reduced skin barrier function (SBF) that might play an important role in its pathophysiology. The SBF is provided primari…
View article: Nonlinear Optics of Skin: Enhancement of Autofluorescence and Second Harmonic Generation Signals by Immersion Optical Clearing
Nonlinear Optics of Skin: Enhancement of Autofluorescence and Second Harmonic Generation Signals by Immersion Optical Clearing Open
Multiphoton microscopy and especially two-photon microscopy are actively used in dermatology for the diagnosis and analysis of skin diseases. The typical skin probing depth for these methods is limited to 150–200 µm due to the strong scatt…
View article: Red- and Near-Infrared-Excited Autofluorescence as a Marker for Acute Oxidative Stress in Skin Exposed to Cigarette Smoke Ex Vivo and In Vivo
Red- and Near-Infrared-Excited Autofluorescence as a Marker for Acute Oxidative Stress in Skin Exposed to Cigarette Smoke Ex Vivo and In Vivo Open
Air pollution is increasing worldwide and skin is exposed to high levels of pollution daily, causing oxidative stress and other negative consequences. The methods used to determine oxidative stress in the skin are invasive and non-invasive…
View article: Human glabrous skin contains crystallized urea dendriform structures in the <i>stratum corneum</i> which affect the hydration levels
Human glabrous skin contains crystallized urea dendriform structures in the <i>stratum corneum</i> which affect the hydration levels Open
Glabrous skin is hair‐free skin with a high density of sweat glands, which is found on the palms, and soles of mammalians, covered with a thick stratum corneum . Dry hands are often an occupational problem which deserves attention from der…
View article: Inhibition of Self-Assembling Peptide Fibrils Formation Using Thioflavin T as a Photosensitizer
Inhibition of Self-Assembling Peptide Fibrils Formation Using Thioflavin T as a Photosensitizer Open
Misfolded proteins produce fibrillar aggregates, which contain β-sheet higher order structures. The oligomers, protofibrils, and fibrils generated during protein aggregation process are cytotoxic and can cause various neurodegenerative dis…
View article: Advanced Skin Antisepsis: Application of UVA-Cleavable Hydroxyethyl Starch Nanocapsules for Improved Eradication of Hair Follicle-Associated Microorganisms
Advanced Skin Antisepsis: Application of UVA-Cleavable Hydroxyethyl Starch Nanocapsules for Improved Eradication of Hair Follicle-Associated Microorganisms Open
Hair follicles constitute important drug delivery targets for skin antisepsis since they contain ≈25% of the skin microbiome. Nanoparticles are known to penetrate deeply into hair follicles. By massaging the skin, the follicular penetratio…
View article: Tattoo Pigments Are Localized Intracellularly in the Epidermis and Dermis of Fresh and Old Tattoos: In vivo Study Using Two-Photon Excited Fluorescence Lifetime Imaging
Tattoo Pigments Are Localized Intracellularly in the Epidermis and Dermis of Fresh and Old Tattoos: In vivo Study Using Two-Photon Excited Fluorescence Lifetime Imaging Open
Background: The knowledge about the location and kinetics of tattoo pigments in human skin after application and during the recovery is restricted due to the limitation of in vivo methods for visualizing pigments. Here, the localization an…
View article: Correlation between Blood Flow and Various Physiological Parameters in Human Skin
Correlation between Blood Flow and Various Physiological Parameters in Human Skin Open
Blood flow is an important parameter of the human organism and skin physiology. It correlates with skin temperature, penetration of active substances through the skin, delivery of antioxidants and development of disease symptoms, especiall…
View article: Label-free imaging of M1 and M2 macrophage phenotypes in the human dermis in vivo using two-photon excited FLIM
Label-free imaging of M1 and M2 macrophage phenotypes in the human dermis in vivo using two-photon excited FLIM Open
Macrophages (ΜΦs) are important immune effector cells that promote (M1 ΜΦs) or inhibit (M2 ΜΦs) inflammation and are involved in numerous physiological and pathogenic immune responses. Their precise role and relevance, however, are not ful…
View article: Confocal Raman Micro-Spectroscopy for Discrimination of Glycerol Diffusivity in Ex Vivo Porcine Dura Mater
Confocal Raman Micro-Spectroscopy for Discrimination of Glycerol Diffusivity in Ex Vivo Porcine Dura Mater Open
Dura mater (DM) is a connective tissue with dense collagen, which is a protective membrane surrounding the human brain. The optical clearing (OC) method was used to make DM more transparent, thereby allowing to increase in-depth investigat…
View article: Glycerol and natural sugar‐derived complex modulate differentially stratum corneum water‐binding properties and structural parameters in an in vitro Raman‐desorption model
Glycerol and natural sugar‐derived complex modulate differentially stratum corneum water‐binding properties and structural parameters in an in vitro Raman‐desorption model Open
The epidermal protective functions are closely associated with skin hydration homeostasis. The understanding of different states of water binding is a rising concept in assessing topically applied formulations and their interaction within …
View article: Quantitative determination of concentration profiles of skin components and topically applied oils by tailored multivariate curve resolution‐alternating least squares using in vivo confocal Raman micro‐spectroscopy
Quantitative determination of concentration profiles of skin components and topically applied oils by tailored multivariate curve resolution‐alternating least squares using in vivo confocal Raman micro‐spectroscopy Open
The main components of the stratum corneum ( SC ), water, lipids, and proteins, are non‐homogeneously distributed throughout the depth. The quantitative determination of their concentration profiles and penetration depth of topically appli…
View article: Penetration Depth of Propylene Glycol, Sodium Fluorescein and Nile Red into the Skin Using Non-Invasive Two-Photon Excited FLIM
Penetration Depth of Propylene Glycol, Sodium Fluorescein and Nile Red into the Skin Using Non-Invasive Two-Photon Excited FLIM Open
The stratum corneum (SC) forms a strong barrier against topical drug delivery. Therefore, understanding the penetration depth and pathways into the SC is important for the efficiency of drug delivery and cosmetic safety. In this study, TPT…
View article: Carotenoids in Human Skin In Vivo: Antioxidant and Photo-Protectant Role against External and Internal Stressors
Carotenoids in Human Skin In Vivo: Antioxidant and Photo-Protectant Role against External and Internal Stressors Open
The antioxidant system of the human body plays a crucial role in maintaining redox homeostasis and has an important protective function. Carotenoids have pronounced antioxidant properties in the neutralization of free radicals. In human sk…
View article: Skin Care Product Rich in Antioxidants and Anti-Inflammatory Natural Compounds Reduces Itching and Inflammation in the Skin of Atopic Dermatitis Patients
Skin Care Product Rich in Antioxidants and Anti-Inflammatory Natural Compounds Reduces Itching and Inflammation in the Skin of Atopic Dermatitis Patients Open
The atopic dermatitis (AD) complex pathogenesis mechanism reveals marked changes of certain signaling factors as well as some morphological alterations in the epidermis. Reduced resilience against environmental factors and oxidative stress…
View article: tMCR‐ALS method for the determination of water concentration profiles in the stratum corneum of untreated and treated skin in vivo
tMCR‐ALS method for the determination of water concentration profiles in the stratum corneum of untreated and treated skin in vivo Open
Skin moisturizing is important in dermatology and cosmetics. Raman microspectroscopy is a well‐suited method to determine water concentration profiles in the stratum corneum (SC) of untreated and formulation‐treated skin in vivo. Until now…
View article: Current Views on Noninvasive in vivo Determination of Physiological Parameters of the Stratum Corneum Using Confocal Raman Microspectroscopy
Current Views on Noninvasive in vivo Determination of Physiological Parameters of the Stratum Corneum Using Confocal Raman Microspectroscopy Open
Confocal Raman microspectroscopy is widely used in dermatology and cosmetology for analysis of the concentration of skin components (lipids, natural moisturizing factor molecules, water) and the penetration depth of cosmetic/medical formul…
View article: Label-free imaging of macrophage phenotypes and phagocytic activity in the human dermis <i>in vivo</i> using two-photon excited FLIM
Label-free imaging of macrophage phenotypes and phagocytic activity in the human dermis <i>in vivo</i> using two-photon excited FLIM Open
Macrophages (MΦs) are important immune effector cells that promote (M1 MΦs) or inhibit (M2 MΦs) inflammation and are involved in numerous physiological and pathogenic immune responses. Their precise role and relevance, however, is not full…