Maximilian C. C. J. C. Ebert
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View article: ATP synthase: a moonlighting enzyme with unprecedented functions
ATP synthase: a moonlighting enzyme with unprecedented functions Open
Novel roles of ATP synthase above and beyond catalysis: a direct participant in energy transduction due to its intrinsic electric field/electrostatic potential.
View article: Functional antibody characterization via direct structural analysis and information‐driven protein–protein docking
Functional antibody characterization via direct structural analysis and information‐driven protein–protein docking Open
Detailed description of the mechanism of action of the therapeutic antibodies is essential for the functional characterization and future optimization of potential clinical agents. We recently developed KD035, a fully human antibody target…
View article: Identification of 3-chymotrypsin like protease (3CLPro) inhibitors as potential anti-SARS-CoV-2 agents
Identification of 3-chymotrypsin like protease (3CLPro) inhibitors as potential anti-SARS-CoV-2 agents Open
Emerging outbreak of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection is a major threat to public health. The morbidity is increasing due to lack of SARS-CoV-2 specific drugs. Herein, we have identified potential drug…
View article: Monitoring protein conformational changes using fluorescent nanoantennas
Monitoring protein conformational changes using fluorescent nanoantennas Open
Fluorescent nanoantennas generated a transient fluorescence "spike" during the hydrolysis of a substrate by calf intestinal alkaline phosphatase (AP). With AP, these fluorescent nanoantennas were used to characterize:1) the hydrolysis of 1…
View article: Dual-Target Inhibitors of the Folate Pathway Inhibit Intrinsically Trimethoprim-Resistant DfrB Dihydrofolate Reductases
Dual-Target Inhibitors of the Folate Pathway Inhibit Intrinsically Trimethoprim-Resistant DfrB Dihydrofolate Reductases Open
Trimethoprim (TMP) is widely used to treat infections in humans and in livestock, accelerating the incidence of TMP resistance. The emergent and largely untracked type II dihydrofolate reductases (DfrBs) are intrinsically TMP-resistant pla…
View article: Indigo Formation and Rapid NADPH Consumption Provide Robust Prediction of Raspberry Ketone Synthesis by Engineered Cytochrome P450 BM3
Indigo Formation and Rapid NADPH Consumption Provide Robust Prediction of Raspberry Ketone Synthesis by Engineered Cytochrome P450 BM3 Open
Natural raspberry ketone has a high value in the flavor, fragrance and pharmaceutical industries. Its extraction is costly, justifying the search for biosynthetic routes. We hypothesized that cytochrome P450 BM3 (P450 BM3) could be enginee…
View article: Structure-Based Design of Dimeric Bisbenzimidazole Inhibitors to an Emergent Trimethoprim-Resistant Type II Dihydrofolate Reductase Guides the Design of Monomeric Analogues
Structure-Based Design of Dimeric Bisbenzimidazole Inhibitors to an Emergent Trimethoprim-Resistant Type II Dihydrofolate Reductase Guides the Design of Monomeric Analogues Open
The worldwide use of the broad-spectrum antimicrobial trimethoprim (TMP) has induced the rise of TMP-resistant microorganisms. In addition to resistance-causing mutations of the microbial chromosomal dihydrofolate reductase (Dfr), the evol…
View article: Enzyme engineering: A synthetic biology approach for more effective library generation and automated high-throughput screening
Enzyme engineering: A synthetic biology approach for more effective library generation and automated high-throughput screening Open
The Golden Gate strategy entails the use of type IIS restriction enzymes, which cut outside of their recognition sequence. It enables unrestricted design of unique DNA fragments that can be readily and seamlessly recombined. Successfully e…
View article: Shifting the boundaries of experimental studies in engineering enzymatic functions : combining the benefits of computational and experimental methods
Shifting the boundaries of experimental studies in engineering enzymatic functions : combining the benefits of computational and experimental methods Open
L'industrie chimique mondiale est en pleine mutation, cherchant des solutions pour rendre la synthèse organique classique plus durable. Une telle solution consiste à passer de la catalyse chimique classique à la biocatalyse. Bien que les a…