Maximilian Fottner
YOU?
Author Swipe
View article: Hijacking a bacterial ABC transporter for genetic code expansion
Hijacking a bacterial ABC transporter for genetic code expansion Open
The site-specific encoding of non-canonical amino acids (ncAAs) provides a powerful tool for expanding the functional repertoire of proteins 1–4 . Its widespread use for basic research and biotechnological applications is, however, hampere…
View article: The functional landscape of the human ubiquitinome
The functional landscape of the human ubiquitinome Open
Protein ubiquitination regulates cell biology through diverse avenues, from quality control-linked protein degradation to signaling functions such as modulating protein-protein interactions and enzyme activation. Mass spectrometry-based pr…
View article: Genetic Code Expansion Facilitates Programmable Ubiquitylation via UBE2W
Genetic Code Expansion Facilitates Programmable Ubiquitylation via UBE2W Open
Deciphering the ubiquitin code requires homogenous, site-specifically ubiquitylated proteins, yet their access remains difficult. Current chemical and enzymatic methods are often limited by harsh conditions, low yields, or the introduction…
View article: Structural basis for the ubiquitin chain recognition of the human 26S proteasome
Structural basis for the ubiquitin chain recognition of the human 26S proteasome Open
Proteasomal degradation is a fundamental process for all eukaryotic life. A protein destined for degradation is first tagged with a polyubiquitin chain, which is selected by the proteasome. Different ubiquitin chain topologies serve as dis…
View article: One‐pot dual protein labeling for simultaneous mechanical and fluorescent readouts in optical tweezers
One‐pot dual protein labeling for simultaneous mechanical and fluorescent readouts in optical tweezers Open
Optical tweezers are widely used in the study of biological macromolecules but are limited by their one‐directional probing capability, potentially missing critical conformational changes. Combining fluorescence microscopy with optical twe…
View article: Hijacking a bacterial membrane transporter for efficient genetic code expansion
Hijacking a bacterial membrane transporter for efficient genetic code expansion Open
The site-specific encoding of non-canonical amino acids (ncAAs) provides a powerful tool for expanding the functional repertoire of proteins. Its widespread use for basic research and biotechnological applications is, however, hampered by …
View article: Genetic Code Expansion Approaches to Decipher the Ubiquitin Code
Genetic Code Expansion Approaches to Decipher the Ubiquitin Code Open
The covalent attachment of Ub (ubiquitin) to target proteins (ubiquitylation) represents one of the most versatile PTMs (post-translational modifications) in eukaryotic cells. Substrate modifications range from a single Ub moiety being att…
View article: One-Pot Dual Protein Labelling for Simultaneous Mechanical and Fluorescent Readouts in Optical Tweezers
One-Pot Dual Protein Labelling for Simultaneous Mechanical and Fluorescent Readouts in Optical Tweezers Open
Optical tweezers are widely used in the study of biological macromolecules but are limited by their one-directional probing capability, potentially missing critical conformational changes. Combining fluorescence microscopy with optical twe…
View article: Deciphering functional roles of protein succinylation and glutarylation using genetic code expansion
Deciphering functional roles of protein succinylation and glutarylation using genetic code expansion Open
View article: Intestinal myofibroblasts regulate intestinal epithelial cell plasticity via YAP/TAZ
Intestinal myofibroblasts regulate intestinal epithelial cell plasticity via YAP/TAZ Open
Intestinal stromal cells play a key role as the crypt niche cells during epithelial homeostasis and tumor initiation. However, the underlying cellular and molecular mechanisms remain unclear. We developed various types of three-dimensional…
View article: Site-Specific Protein Labeling and Generation of Defined Ubiquitin-Protein Conjugates Using an Asparaginyl Endopeptidase
Site-Specific Protein Labeling and Generation of Defined Ubiquitin-Protein Conjugates Using an Asparaginyl Endopeptidase Open
Asparaginyl endopeptidases (AEPs) have recently been widely utilized for peptide and protein modification. Labeling is however restricted to protein termini, severely limiting flexibility and scope in creating diverse conjugates as needed …
View article: A modular toolbox to generate complex polymeric ubiquitin architectures using orthogonal sortase enzymes
A modular toolbox to generate complex polymeric ubiquitin architectures using orthogonal sortase enzymes Open
View article: Site‐Specific Protein Labeling with Fluorophores as a Tool To Monitor Protein Turnover
Site‐Specific Protein Labeling with Fluorophores as a Tool To Monitor Protein Turnover Open
Proteins that terminally fail to acquire their native structure are detected and degraded by cellular quality control systems. Insights into cellular protein quality control are key to a better understanding of how cells establish and main…
View article: Modification and Functionalization of the Guanidine Group by Tailor-made Precursors
Modification and Functionalization of the Guanidine Group by Tailor-made Precursors Open
The guanidine group is one of the most important pharmacophoric groups in medicinal chemistry. The only amino acid carrying a guanidine group is arginine. In this article, an easy method for the modification of the guanidine group in pepti…