Maximilian Wranik
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View article: Time-resolved structures of β<sub>2</sub>-adrenergic receptor modulation by a photoswitchable beta-blocker
Time-resolved structures of β<sub>2</sub>-adrenergic receptor modulation by a photoswitchable beta-blocker Open
G protein-coupled receptors (GPCRs) regulate essential physiological responses and are important drug targets, yet their ligand-induced conformational dynamics remain poorly understood. The β 2 -adrenergic receptor (β 2 AR) is a prominent …
View article: Capturing macromolecular dynamics using time-resolved serial crystallography at X- ray free electron lasers and synchrotron light sources
Capturing macromolecular dynamics using time-resolved serial crystallography at X- ray free electron lasers and synchrotron light sources Open
In recent years, time-resolved serial crystallography has emerged as a transformative technique for unraveling the intricate dynamics of macromolecules at atomic resolution. By leveraging the high-intensity and ultra-short pulses of X-ray …
View article: Structural basis for the dynamic regulation of mTORC1 by amino acids
Structural basis for the dynamic regulation of mTORC1 by amino acids Open
The mechanistic target of rapamycin complex 1 (mTORC1) anchors a conserved signalling pathway that regulates growth in response to nutrient availability1-5. Amino acids activate mTORC1 through the Rag GTPases, which are regulate…
View article: Photoswitch dissociation from a G protein-coupled receptor resolved by time-resolved serial crystallography
Photoswitch dissociation from a G protein-coupled receptor resolved by time-resolved serial crystallography Open
View article: A photo-SAR study of photoswitchable azobenzene tubulin-inhibiting antimitotics identifying a general method for near-quantitative photocontrol
A photo-SAR study of photoswitchable azobenzene tubulin-inhibiting antimitotics identifying a general method for near-quantitative photocontrol Open
Here, we performed photo-SAR studies in a series of photoswitchable tubulin inhibiting antimitotics, and discovered that fluorescent antenna motifs permit their single-photon photoisomerisation in biological conditions at up to >600 nm; we…
View article: A photo-SAR study of photoswitchable azobenzene tubulin-inhibiting antimitotics identifying a general method for near-quantitative photocontrol
A photo-SAR study of photoswitchable azobenzene tubulin-inhibiting antimitotics identifying a general method for near-quantitative photocontrol Open
We develop reagents to optically control microtubule dynamics, with (1) lensless microscopy to track their bioactivity; (2) photo-SAR to find GFP-compatible reagents; & (3) fluorophore antennas to drive single-photon isomerisation even ≥60…
View article: A multi-reservoir extruder for time-resolved serial protein crystallography and compound screening at X-ray free-electron lasers
A multi-reservoir extruder for time-resolved serial protein crystallography and compound screening at X-ray free-electron lasers Open
Serial crystallography at X-ray free-electron lasers (XFELs) permits the determination of radiation-damage free static as well as time-resolved protein structures at room temperature. Efficient sample delivery is a key factor for such expe…
View article: Visualizing the DNA repair process by a photolyase at atomic resolution
Visualizing the DNA repair process by a photolyase at atomic resolution Open
Photolyases, a ubiquitous class of flavoproteins, use blue light to repair DNA photolesions. In this work, we determined the structural mechanism of the photolyase-catalyzed repair of a cyclobutane pyrimidine dimer (CPD) lesion using time-…
View article: Time-resolved crystallography captures light-driven DNA repair
Time-resolved crystallography captures light-driven DNA repair Open
Photolyase is an enzyme that uses light to catalyze DNA repair. To capture the reaction intermediates involved in the enzyme’s catalytic cycle, we conducted a time-resolved crystallography experiment. We found that photolyase traps the exc…
View article: A multi-reservoir extruder for time-resolved serial protein crystallography and compound screening at X-ray free-electron lasers
A multi-reservoir extruder for time-resolved serial protein crystallography and compound screening at X-ray free-electron lasers Open
Serial crystallography at X-ray free-electron lasers (XFELs) permits the determination of radiation-damage free static as well as time-resolved protein structures at room temperature. Efficient sample delivery is a key factor for such expe…
View article: Watching the release of a photopharmacological drug from tubulin using time-resolved serial crystallography
Watching the release of a photopharmacological drug from tubulin using time-resolved serial crystallography Open
View article: Release of a photopharmacological drug from its protein target captured by time-resolved serial crystallography
Release of a photopharmacological drug from its protein target captured by time-resolved serial crystallography Open
The binding and release of ligands from their protein targets is central to fundamental biological processes as well as to drug discovery. Photopharmacology introduces chemical triggers that allow the changing of ligand affinities and thus…
View article: <i>In Vivo</i> Photocontrol of Microtubule Dynamics and Integrity, Migration and Mitosis, by the Potent GFP-Imaging-Compatible Photoswitchable Reagents SBTubA4P and SBTub2M
<i>In Vivo</i> Photocontrol of Microtubule Dynamics and Integrity, Migration and Mitosis, by the Potent GFP-Imaging-Compatible Photoswitchable Reagents SBTubA4P and SBTub2M Open
Photoswitchable reagents are powerful tools for high-precision studies in cell biology. When these reagents are globally administered yet locally photoactivated in two-dimensional (2D) cell cultures, they can exert micron- and millisecond-…
View article: Dynamics and mechanism of a light-driven chloride pump
Dynamics and mechanism of a light-driven chloride pump Open
Chloride transport by microbial rhodopsins is an essential process for which molecular details such as the mechanisms that convert light energy to drive ion pumping and ensure the unidirectionality of the transport have remained elusive. W…
View article: Watching the release of a photopharmacological drug from tubulin using time-resolved serial crystallography
Watching the release of a photopharmacological drug from tubulin using time-resolved serial crystallography Open
The binding and release of ligands from their protein targets is central to fundamental biological processes as well as to drug discovery. Photopharmacology introduces chemical triggers that allow the changing of ligand affinities and thus…
View article: Structural basis of the radical pair state in photolyases and cryptochromes
Structural basis of the radical pair state in photolyases and cryptochromes Open
The structural response of the drosophila (6-4) photolyase to photoinduced electron transfer along a chain of tryptophans is revealed using a serial crystallographic snapshot of the protein in its radical pair state.
View article: Versatile microporous polymer-based supports for serial macromolecular crystallography
Versatile microporous polymer-based supports for serial macromolecular crystallography Open
Serial data collection has emerged as a major tool for data collection at state-of-the-art light sources, such as microfocus beamlines at synchrotrons and X-ray free-electron lasers. Challenging targets, characterized by small crystal size…
View article: In vivo photocontrol of microtubule dynamics and integrity, migration and mitosis, by the potent GFP-imaging-compatible photoswitchable reagents SBTubA4P and SBTub2M
In vivo photocontrol of microtubule dynamics and integrity, migration and mitosis, by the potent GFP-imaging-compatible photoswitchable reagents SBTubA4P and SBTub2M Open
Photoswitchable reagents to modulate microtubule stability and dynamics are an exciting tool approach towards micron- and millisecond-scale control over endogenous cytoskeleton-dependent processes. When these reagents are globally administ…
View article: A Robust, GFP-Orthogonal Photoswitchable Inhibitor Scaffold Extends Optical Control over the Microtubule Cytoskeleton
A Robust, GFP-Orthogonal Photoswitchable Inhibitor Scaffold Extends Optical Control over the Microtubule Cytoskeleton Open
View article: Photoswitchable microtubule inhibitors enabling robust, GFP-orthogonal optical control over the tubulin cytoskeleton
Photoswitchable microtubule inhibitors enabling robust, GFP-orthogonal optical control over the tubulin cytoskeleton Open
Here we present GFP-orthogonal optically controlled reagents for reliable and repetitive in cellulo modulation of microtubule dynamics and its dependent processes. Optically controlled reagents (“photopharmaceuticals”) have developed into …