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View article: Leveraging CRISPR activation for rapid assessment of gene editing products in human pluripotent stem cells
Leveraging CRISPR activation for rapid assessment of gene editing products in human pluripotent stem cells Open
Verification of genome editing in human pluripotent stem cells (hPSCs), particularly at silent loci, is desirable but challenging, as it often requires complex and time-intensive differentiation to induce their expression. Here, we establi…
View article: Retinoid X Receptor Signaling Mediates Cancer Cell Lipid Metabolism in the Leptomeninges
Retinoid X Receptor Signaling Mediates Cancer Cell Lipid Metabolism in the Leptomeninges Open
Summary Cancer cells metastatic to the leptomeninges encounter a metabolically-challenging extreme microenvironment. To understand adaptations to this space, we subjected leptomeningeal-metastatic (LeptoM) mouse breast and lung cancers iso…
View article: A robust and inducible precise genome editing via an all-in-one prime editor in human pluripotent stem cells
A robust and inducible precise genome editing via an all-in-one prime editor in human pluripotent stem cells Open
Prime editing (PE) allows for precise genome editing in human pluripotent stem cells (hPSCs), such as introducing single nucleotide modifications, small deletions, or insertions at a specific genomic locus, a strategy that shows great prom…
View article: Protocol for the design, conduct, and evaluation of prime editing in human pluripotent stem cells
Protocol for the design, conduct, and evaluation of prime editing in human pluripotent stem cells Open
Prime editing introduces single-nucleotide polymorphism changes, small deletions, or insertions at a specific genome site without double-stranded DNA breaks or the need for the donor template. Here, we present a protocol to design, conduct…
View article: hPSC-derived sacral neural crest enables rescue in a severe model of Hirschsprung’s disease
hPSC-derived sacral neural crest enables rescue in a severe model of Hirschsprung’s disease Open
The enteric nervous system (ENS) is derived from both the vagal and sacral component of the neural crest (NC). Here, we present the derivation of sacral ENS precursors from human PSCs via timed exposure to FGF, WNT, and GDF11, which enable…