Megan C. King
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View article: Presence 5 for trauma informed care: teaching tangible practices towards bidirectional healing in undergraduate medical education
Presence 5 for trauma informed care: teaching tangible practices towards bidirectional healing in undergraduate medical education Open
Introduction Trauma impacts health; the adverse health effects of trauma are well-understood and trauma-informed care is recommended to mitigate these effects. There is an opportunity to leverage evidence-based frameworks to add to the gro…
View article: Contemporary Dissemination Rates Among Research Presented at Obstetrics and Gynecology Subspecialty Conferences
Contemporary Dissemination Rates Among Research Presented at Obstetrics and Gynecology Subspecialty Conferences Open
OBJECTIVE: We aimed to evaluate the conversion rate of conference abstract presentation to full manuscript among obstetrics and gynecology subspecialty society meetings. METHODS: We conducted a retrospective cohort study of 2019 meeting su…
View article: First-passage time to capture for diffusion in a 3D harmonic potential
First-passage time to capture for diffusion in a 3D harmonic potential Open
We determine the survival probability and first-passage time (FPT) to capture for a harmonically trapped particle, diffusing outside an absorbing spherical boundary by directly solving the differential equation for the survival probability…
View article: The LINC complex component Kms1 and CENP-B protein Cbp1 cooperate to enforce faithful homology-directed DNA repair at the nuclear periphery in <i>S. pombe</i>
The LINC complex component Kms1 and CENP-B protein Cbp1 cooperate to enforce faithful homology-directed DNA repair at the nuclear periphery in <i>S. pombe</i> Open
While homologous recombination (HR) is often considered to be an error-free DNA repair mechanism, the fidelity of this pathway depends on the cell’s ability to engage the ideal template, the replicated sister chromatid. This is particularl…
View article: Coarse-grained chromatin dynamics by tracking multiple similarly labeled gene loci
Coarse-grained chromatin dynamics by tracking multiple similarly labeled gene loci Open
The “holy grail” of chromatin research would be to follow the chromatin configuration in individual live cells over time. One way to achieve this goal would be to track the positions of multiple loci arranged along the chromatin polymer wi…
View article: Loss of JAK1 Function Causes G2/M Cell Cycle Defects Vulnerable to Kif18a Inhibition
Loss of JAK1 Function Causes G2/M Cell Cycle Defects Vulnerable to Kif18a Inhibition Open
To gain insight into biological mechanisms that cause resistance to DNA damage, we performed parallel pooled genetic CRISPR-Cas9 screening for survival in high risk HNSCC subtypes. Surprisingly, and in addition to ATM, DNAPK, and NFKB sign…
View article: Cell cycle regulation of polymerase theta mediated end-joining underlies its sensitivity to loss of BLM-DNA2
Cell cycle regulation of polymerase theta mediated end-joining underlies its sensitivity to loss of BLM-DNA2 Open
DNA double-strand breaks (DSBs) are predominantly resolved by the error-prone non-homologous end-joining (NHEJ) or high-fidelity homologous recombination (HR) DNA repair pathways. NHEJ involves minimal end-processing and drives direct liga…
View article: Visualizing nuclear pore complex plasticity with pan-Expansion Microscopy
Visualizing nuclear pore complex plasticity with pan-Expansion Microscopy Open
The exploration of cell-type and environmentally-responsive nuclear pore complex (NPC) plasticity requires new, accessible tools. Using pan-Expansion Microscopy (pan-ExM), NPCs were identified by machine learning-facilitated segmentation w…
View article: Identifying topologically associating domains using differential kernels
Identifying topologically associating domains using differential kernels Open
Chromatin is a polymer complex of DNA and proteins that regulates gene expression. The three-dimensional (3D) structure and organization of chromatin controls DNA transcription and replication. High-throughput chromatin conformation captur…
View article: eLife Assessment: Treacle’s ability to form liquid phase condensates is essential for nucleolar fibrillar center assembly, efficient rRNA transcription and processing, and rRNA gene repair
eLife Assessment: Treacle’s ability to form liquid phase condensates is essential for nucleolar fibrillar center assembly, efficient rRNA transcription and processing, and rRNA gene repair Open
We investigated the role of the nucleolar protein Treacle in organizing and regulating the nucleolus in human cells. Our results support Treacle's capacity to form liquid-phase condensates through electrostatic interactions among molecules…
View article: eLife Assessment: An Hfq-dependent post-transcriptional mechanism fine tunes RecB expression in Escherichia coli
eLife Assessment: An Hfq-dependent post-transcriptional mechanism fine tunes RecB expression in Escherichia coli Open
All living organisms have developed strategies to respond to chromosomal damage and preserve genome integrity. One such response is the repair of DNA double-strand breaks (DSBs), one of the most toxic forms of DNA lesions. In Escherichia c…
View article: eLife Assessment: Mitosis sets nuclear homeostasis of cancer cells under confinement
eLife Assessment: Mitosis sets nuclear homeostasis of cancer cells under confinement Open
During their life, mammalian cells are subjected to numerous mechanical constraints, especially in pathological contexts such as cancer. Recent studies have highlighted the central role of the nucleus in sensing mechanical cues, but they o…
View article: A quantitative ultrastructural timeline of nuclear autophagy reveals a role for dynamin-like protein 1 at the nuclear envelope
A quantitative ultrastructural timeline of nuclear autophagy reveals a role for dynamin-like protein 1 at the nuclear envelope Open
Autophagic mechanisms that maintain nuclear envelope homeostasis are bulwarks to aging and disease. By leveraging 4D lattice light sheet microscopy and correlative light and electron tomography, we define a quantitative and ultrastructural…
View article: Identifying topologically associating domains using differential kernels
Identifying topologically associating domains using differential kernels Open
Chromatin is a polymer complex of DNA and proteins that regulates gene expression. The three-dimensional structure and organization of chromatin controls DNA transcription and replication. High-throughput chromatin conformation capture tec…
View article: It is time to reconsider the culture of volunteerism in undergraduate research
It is time to reconsider the culture of volunteerism in undergraduate research Open
One of the empowering aspects of serving as a mentor is the space it provides for me to reflect on my own experience and to leverage my position to improve the often-hidden systems underlying academia for the next generation. Recently, it …
View article: eLife Assessment: Alternative end-joining results in smaller deletions in heterochromatin relative to euchromatin
eLife Assessment: Alternative end-joining results in smaller deletions in heterochromatin relative to euchromatin Open
Pericentromeric heterochromatin is highly enriched for repetitive sequences prone to aberrant recombination. Previous studies showed that homologous recombination (HR) repair is uniquely regulated in this domain to enable ‘safe’ repair whi…
View article: Cohesin distribution alone predicts chromatin organization in yeast via conserved-current loop extrusion
Cohesin distribution alone predicts chromatin organization in yeast via conserved-current loop extrusion Open
Inhomogeneous patterns of enhanced chromatin-chromatin contacts within 10-100 kb-sized regions of the genome are a generic feature of chromatin spatial organization. These features, termed topologically associating domains (TADs), have led…
View article: Joint Public Review: High-precision mapping of nuclear pore-chromatin interactions reveals new principles of genome organization at the nuclear envelope
Joint Public Review: High-precision mapping of nuclear pore-chromatin interactions reveals new principles of genome organization at the nuclear envelope Open
The role of nuclear pore complexes (NPCs) in genome organization remains poorly characterized due to technical limitations in probing genome-wide protein-DNA interactions specific to the nuclear periphery. Here, we developed a new sensitiv…
View article: eLife Assessment: High-precision mapping of nuclear pore-chromatin interactions reveals new principles of genome organization at the nuclear envelope
eLife Assessment: High-precision mapping of nuclear pore-chromatin interactions reveals new principles of genome organization at the nuclear envelope Open
The role of nuclear pore complexes (NPCs) in genome organization remains poorly characterized due to technical limitations in probing genome-wide protein-DNA interactions specific to the nuclear periphery. Here, we developed a new sensitiv…
View article: Loops and the activity of loop extrusion factors constrain chromatin dynamics
Loops and the activity of loop extrusion factors constrain chromatin dynamics Open
The relationship between chromatin structure and dynamics is incompletely understood. Here experimental and computational approaches are combined to reveal that looping interactions impose constraints on chromatin mobility while the ATP-de…
View article: The effect of loops on the mean square displacement of Rouse-model chromatin
The effect of loops on the mean square displacement of Rouse-model chromatin Open
Many researchers have been encouraged to describe the dynamics of chromosomal loci in chromatin using the classical Rouse model of polymer dynamics by the agreement between the measured mean square displacement (MSD) versus time of fluores…
View article: Elasticity of spheres with buckled surfaces
Elasticity of spheres with buckled surfaces Open
The buckling instabilities of core-shell systems, comprising an interior elastic sphere, attached to an exterior shell, have been proposed to underlie myriad biological morphologies. To fully discuss such systems, however, it is important …
View article: An ESCRT grommet cooperates with a diffusion barrier to maintain nuclear integrity
An ESCRT grommet cooperates with a diffusion barrier to maintain nuclear integrity Open
The molecular mechanisms by which the endosomal sorting complexes required for transport (ESCRT) proteins contribute to the integrity of the nuclear envelope (NE) barrier are not fully defined. Here, we leveraged the single NE hole generat…