Mel Β. Feany
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View article: Characterization of cognitive decline in long-duration type 1 diabetes by cognitive, neuroimaging, and pathological examinations
Characterization of cognitive decline in long-duration type 1 diabetes by cognitive, neuroimaging, and pathological examinations Open
BACKGROUNDWe aimed to characterize factors associated with the under-studied complication of cognitive decline in aging people with long-duration type 1 diabetes (T1D).METHODSJoslin "Medalists" (n = 222; T1D ≥ 50 years) underwent cognitive…
View article: The Biological Intersection Between Chemotherapy-Related Cognitive Impairment and Alzheimer Disease
The Biological Intersection Between Chemotherapy-Related Cognitive Impairment and Alzheimer Disease Open
Alzheimer disease (AD) is the most common type of dementia and one of the leading causes of death in elderly patients. The number of patients with AD in the United States is projected to double by 2060. Thus, understanding modifiable risk …
View article: Transcriptional programs mediating neuronal toxicity and altered glial–neuronal signaling in a <i>Drosophila</i> knock-in tauopathy model
Transcriptional programs mediating neuronal toxicity and altered glial–neuronal signaling in a <i>Drosophila</i> knock-in tauopathy model Open
Missense mutations in the gene encoding the microtubule-associated protein TAU (current and approved symbol is MAPT) cause autosomal dominant forms of frontotemporal dementia. Multiple models of frontotemporal dementia based on transgenic …
View article: An integrative systems-biology approach defines mechanisms of Alzheimer’s disease neurodegeneration
An integrative systems-biology approach defines mechanisms of Alzheimer’s disease neurodegeneration Open
Despite years of intense investigation, the mechanisms underlying neuronal death in Alzheimer’s disease, the most common neurodegenerative disorder, remain incompletely understood. To define relevant pathways, we integrated the results of …
View article: Deep sequencing of proteotoxicity modifier genes uncovers a Presenilin-2/beta-amyloid-actin genetic risk module shared among alpha-synucleinopathies
Deep sequencing of proteotoxicity modifier genes uncovers a Presenilin-2/beta-amyloid-actin genetic risk module shared among alpha-synucleinopathies Open
Whether neurodegenerative diseases linked to misfolding of the same protein share genetic risk drivers or whether different protein-aggregation pathologies in neurodegeneration are mechanistically related remains uncertain. Conventional ge…
View article: Transcriptional programs mediating neuronal toxicity and altered glial-neuronal signaling in a<i>Drosophila</i>knock-in tauopathy model
Transcriptional programs mediating neuronal toxicity and altered glial-neuronal signaling in a<i>Drosophila</i>knock-in tauopathy model Open
Missense mutations in the gene encoding the microtubule-associated protein tau cause autosomal dominant forms of frontotemporal dementia. Multiple models of frontotemporal dementia based on transgenic expression of human tau in experimenta…
View article: DNA damage assessment in the adult Drosophila brain via comet assay v1
DNA damage assessment in the adult Drosophila brain via comet assay v1 Open
This protocol describes how to determine DNA damage in the adult drosophila brain using the comet assay
View article: Assessment of autophagic flux assay in the adult Drosophila brain v1
Assessment of autophagic flux assay in the adult Drosophila brain v1 Open
Transgenic flies expressing GFP-mCherry-Atg8a (obtained from the BloomingtonDrosophilaStock Center) under the control of theneuronal nSyb-GAL4 driver are used for assaying autophagic flux.
View article: Labeling lysosomes in the adult Drosophila brain using LysoTracker v1
Labeling lysosomes in the adult Drosophila brain using LysoTracker v1 Open
This protocol describes the labeling and quantification of Lysosomes in the adult Drosophila brain using the LysoTracker dye
View article: Pathways controlling neurotoxicity and proteostasis in mitochondrial complex I deficiency
Pathways controlling neurotoxicity and proteostasis in mitochondrial complex I deficiency Open
Neuromuscular disorders caused by dysfunction of the mitochondrial respiratory chain are common, severe and untreatable. We recovered a number of mitochondrial genes, including electron transport chain components, in a large forward geneti…
View article: DNA damage assessment in the adult Drosophila brain via pH2Av (S137) immunostaining v1
DNA damage assessment in the adult Drosophila brain via pH2Av (S137) immunostaining v1 Open
This protocol describes how to stain adult Drosophila brains with pH2Av to assess DNA damage.
View article: Single cell sequencing preparation in adult Drosophila Brain v1
Single cell sequencing preparation in adult Drosophila Brain v1 Open
This protocol covers the preparation of adult DrosophilaBrains for single cell RNAseq
View article: Pathways controlling neurotoxicity and proteostasis in mitochondrial complex I deficiency
Pathways controlling neurotoxicity and proteostasis in mitochondrial complex I deficiency Open
Neuromuscular disorders caused by dysfunction of the mitochondrial respiratory chain are common, severe and untreatable. We recovered a number of mitochondrial genes, including electron transport chain components, in a large forward geneti…
View article: Integrative analysis reveals a conserved role for the amyloid precursor protein in proteostasis during aging
Integrative analysis reveals a conserved role for the amyloid precursor protein in proteostasis during aging Open
Aβ peptides derived from the amyloid precursor protein (APP) have been strongly implicated in the pathogenesis of Alzheimer’s disease. However, the normal function of APP and the importance of that role in neurodegenerative disease is less…
View article: Integrative analysis reveals a conserved role for the amyloid precursor protein in proteostasis during aging
Integrative analysis reveals a conserved role for the amyloid precursor protein in proteostasis during aging Open
Aβ peptides derived from the amyloid precursor protein (APP) have been strongly implicated in the pathogenesis of Alzheimer’s disease. However, the normal function of APP and the importance of that role in neurodegenerative disease is less…
View article: Drosophila Locomotion Protocol v1
Drosophila Locomotion Protocol v1 Open
This protocol is used to assay the locomotion of flies as a screening tool.
View article: α-synuclein promotes neuronal dysfunction and death by disrupting the binding of ankyrin to ß-spectrin
α-synuclein promotes neuronal dysfunction and death by disrupting the binding of ankyrin to ß-spectrin Open
α-synuclein plays a key role in the pathogenesis of Parkinson’s disease and related disorders, but critical interacting partners and molecular mechanisms mediating neurotoxicity are incompletely understood. We show that α-synuclein binds d…
View article: A<i>Drosophila</i>model of chemotherapy-related cognitive impairment
A<i>Drosophila</i>model of chemotherapy-related cognitive impairment Open
Chemotherapy-related cognitive impairment (CRCI) is a common adverse effect of treatment and is characterized by deficits involving multiple cognitive domains including memory. Despite the significant morbidity of CRCI and the expected inc…
View article: Circular RNAs in the human brain are tailored to neuron identity and neuropsychiatric disease
Circular RNAs in the human brain are tailored to neuron identity and neuropsychiatric disease Open
Little is known about circular RNAs (circRNAs) in specific brain cells and human neuropsychiatric disease. Here, we systematically identified over 11,039 circRNAs expressed in vulnerable dopamine and pyramidal neurons laser-captured from 1…
View article: STAT3 Drives GFAP Accumulation and Astrocyte Pathology in a Mouse Model of Alexander Disease
STAT3 Drives GFAP Accumulation and Astrocyte Pathology in a Mouse Model of Alexander Disease Open
Alexander disease (AxD) is caused by mutations in the gene for glial fibrillary acidic protein (GFAP), an intermediate filament expressed by astrocytes in the central nervous system. AxD-associated mutations cause GFAP aggregation and astr…
View article: α-Synuclein Promotes Neuronal Dysfunction and Death by Disrupting the Binding of Ankyrin to β-Spectrin
α-Synuclein Promotes Neuronal Dysfunction and Death by Disrupting the Binding of Ankyrin to β-Spectrin Open
α-Synuclein plays a key role in the pathogenesis of Parkinson’s disease and related disorders, but critical interacting partners and molecular mechanisms mediating neurotoxicity are incompletely understood. We show that α-synuclein binds d…
View article: Nicotine‐Mediated Rescue of α‐Synuclein Toxicity Requires Synaptic Vesicle Glycoprotein 2 in <i>Drosophila</i>
Nicotine‐Mediated Rescue of α‐Synuclein Toxicity Requires Synaptic Vesicle Glycoprotein 2 in <i>Drosophila</i> Open
Background Parkinson's disease (PD) is characterized by α‐synuclein aggregation and loss of dopamine neurons. Risk of PD arises due to a combination of genetic and environmental factors, which may interact, termed gene–environment (G×E) in…
View article: Fly Model Protocols v1
Fly Model Protocols v1 Open
A collection of protocols describing experimental work with fly models.
View article: Western Blotting (Fly Heads) v2
Western Blotting (Fly Heads) v2 Open
This protocol describes how to perform a Western Blotting technique using fly heads.
View article: Immunostaining on Paraffin Sections of Fly Heads v2
Immunostaining on Paraffin Sections of Fly Heads v2 Open
This protocol describes how to perform immunostaining on paraffin sections of fly heads.