Mélanie Berbon
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View article: Synthetic α-synuclein fibrils replicate in mice causing MSA-like pathology
Synthetic α-synuclein fibrils replicate in mice causing MSA-like pathology Open
Multiple-system atrophy (MSA) is a rapidly progressive neurodegenerative disease of unknown cause, typically affecting individuals aged 50-60 years and leading to death within a decade1-3. It is characterized by glial cytoplasmic inclusion…
View article: <i>Aspergillus fumigatus</i> effector crpA orchestrates host prostaglandin signaling to promote fungal virulence
<i>Aspergillus fumigatus</i> effector crpA orchestrates host prostaglandin signaling to promote fungal virulence Open
Conidia are the primary infection structures in Aspergillus fumigatus , the etiologic agent of aspergillosis. Here, we characterized CrpA (a c ysteine- r ich p rotein), a conidial surface-associated protein important for fungal evasion and…
View article: Identification of widely conserved biosynthetic gene cluster involved in pigment production of <i>Bacillus subtilis</i>
Identification of widely conserved biosynthetic gene cluster involved in pigment production of <i>Bacillus subtilis</i> Open
Bacillus subtilis is widely studied in the microbial secondary metabolite (SM) field due to its rich variety of important natural products and genetic tractability. We report a pigment observed in B. subtilis soil isolate MB9_B4 on certain…
View article: Molecular Distinction of Cell Wall and Capsular Polysaccharides in Encapsulated Pathogens by In Situ Magic-Angle Spinning NMR Techniques
Molecular Distinction of Cell Wall and Capsular Polysaccharides in Encapsulated Pathogens by In Situ Magic-Angle Spinning NMR Techniques Open
Pathogenic fungal and bacterial cells are enveloped within a cell wall, a molecular barrier at their cell surface, and a critical architecture that constantly evolves during pathogenesis. Understanding the molecular composition, structural…
View article: RePo-SUDOE: A Transnational Network for Drug Repurposing in the SUDOE Space
RePo-SUDOE: A Transnational Network for Drug Repurposing in the SUDOE Space Open
The development of new drugs is hindered by high failure rates, substantial costs, and prolonged timelines, highlighting the importance of drug repurposing (or drug repositioning) as a key strategy to identify new therapeutic applications …
View article: Characterization of widely conserved novel pigment production in<i>Bacillus subtilis</i>species
Characterization of widely conserved novel pigment production in<i>Bacillus subtilis</i>species Open
Bacillus subtilis is widely studied in the microbial secondary metabolite (SM) field due to its rich variety of important natural products and genetic tractability. However, identification of novel SMs and their biosynthetic gene cluster (…
View article: Bacterial flotillins as destabilizers of phospholipid membranes
Bacterial flotillins as destabilizers of phospholipid membranes Open
From archaea to mammals evolutionary conserved flotillins are scaffolding proteins, recognized for their nandomain-segregating activity. Flotillins form basket-like oligomeric architectures on the membrane, based on a conserved secondary s…
View article: Amyloid signaling in antiphage defense
Amyloid signaling in antiphage defense Open
Immune regulated cell death (RCD) is a defense strategy common to different domains of life involving purposeful sacrifice of infected cells. In animals and fungi, functional amyloids play a role in the control of RCD as molecular switches…
View article: Magic-angle spinning NMR spectral editing of polysaccharides in whole cells using the DREAM scheme
Magic-angle spinning NMR spectral editing of polysaccharides in whole cells using the DREAM scheme Open
Most bacterial, plant and fungal cells possess at their surface a protective layer called the cell wall, conferring strength, plasticity and rigidity to withstand the osmotic pressure. This molecular barrier is crucial for pathogenic micro…
View article: Synthetic α-synuclein fibrils replicate in mice causing MSA-like pathology
Synthetic α-synuclein fibrils replicate in mice causing MSA-like pathology Open
Summary Paragraph Multiple system atrophy (MSA) is a rapidly progressive neurodegenerative disease of unknown cause, typically affecting individuals aged 50–60 and leading to death within a decade 1–3 . It is characterized by glial cytopla…
View article: Dynamic pre-structuration of lipid nanodomain-segregating remorin proteins
Dynamic pre-structuration of lipid nanodomain-segregating remorin proteins Open
Remorins are multifunctional proteins, regulating immunity, development and symbiosis in plants. When associating to the membrane, remorins sequester specific lipids into functional membrane nanodomains. The multigenic protein family conta…
View article: Analytical Framework to Understand the Origins of Methyl Side-Chain Dynamics in Protein Assemblies
Analytical Framework to Understand the Origins of Methyl Side-Chain Dynamics in Protein Assemblies Open
Side-chain motions play an important role in understanding protein structure, dynamics, protein-protein, and protein-ligand interactions. However, our understanding of protein side-chain dynamics is currently limited by the lack of analyti…
View article: CapP mediates the structural formation of biofilm-specific pili in the opportunistic human pathogen <i>Bacillus cereus</i>
CapP mediates the structural formation of biofilm-specific pili in the opportunistic human pathogen <i>Bacillus cereus</i> Open
SUMMARY Polymeric proteinaceous filaments are structural scaffolds that diversify the functionality of the bacterial extracellular matrix. Here, we report a previously uncharacterized bacterial factor called bc1280 that is exclusive to B. …
View article: Hfq C-terminal region forms a β-rich amyloid-like motif without perturbing the N-terminal Sm-like structure
Hfq C-terminal region forms a β-rich amyloid-like motif without perturbing the N-terminal Sm-like structure Open
Hfq is a pleitropic actor, serving as stress response and virulence factor in the bacterial cell. It achieves this function mostly through its binding to small noncoding RNAs, conferring annealing with mRNAs. To execute these functions, Hf…
View article: Cover Picture: The Rigid Core and Flexible Surface of Amyloid Fibrils Probed by Magic‐Angle‐Spinning NMR Spectroscopy of Aromatic Residues (Angew. Chem. Int. Ed. 19/2023)
Cover Picture: The Rigid Core and Flexible Surface of Amyloid Fibrils Probed by Magic‐Angle‐Spinning NMR Spectroscopy of Aromatic Residues (Angew. Chem. Int. Ed. 19/2023) Open
Rock-solid amyloid?. Aromatic residues in the core of the amyloid fibrils HELLF and HET-s show no sign of ring flips suggesting a rigid core that does not undergo "breathing motions". Aromatic residues facing outside on the other hand are …
View article: Structural polymorphism of the low-complexity C-terminal domain of TDP-43 amyloid aggregates revealed by solid-state NMR
Structural polymorphism of the low-complexity C-terminal domain of TDP-43 amyloid aggregates revealed by solid-state NMR Open
Aberrant aggregation of the transactive response DNA-binding protein (TDP-43) is associated with several lethal neurodegenerative diseases, including amyotrophic lateral sclerosis and frontotemporal dementia. Cytoplasmic neuronal inclusion…
View article: The Rigid Core and Flexible Surface of Amyloid Fibrils Probed by Magic‐Angle‐Spinning NMR Spectroscopy of Aromatic Residues
The Rigid Core and Flexible Surface of Amyloid Fibrils Probed by Magic‐Angle‐Spinning NMR Spectroscopy of Aromatic Residues Open
Aromatic side chains are important reporters of the plasticity of proteins, and often form important contacts in protein–protein interactions. We studied aromatic residues in the two structurally homologous cross‐β amyloid fibrils HET‐s, a…
View article: Cell-free synthesis of amyloid fibrils with infectious properties and amenable to sub-milligram magic-angle spinning NMR analysis
Cell-free synthesis of amyloid fibrils with infectious properties and amenable to sub-milligram magic-angle spinning NMR analysis Open
Structural investigations of amyloid fibrils often rely on heterologous bacterial overexpression of the protein of interest. Due to their inherent hydrophobicity and tendency to aggregate as inclusion bodies, many amyloid proteins are chal…
View article: Efficient high-field MAS-DNP NMR reveals hidden side chains in amyloid fibrils
Efficient high-field MAS-DNP NMR reveals hidden side chains in amyloid fibrils Open
It has been widely observed in bioNMR experiments that many biological molecules contain flexible parts or side chains that do not yield easily observable NMR signals in room-temperature experiments. The reasons for the missing peaks could…
View article: Structures of Pathological and Functional Amyloids and Prions, a Solid-State NMR Perspective
Structures of Pathological and Functional Amyloids and Prions, a Solid-State NMR Perspective Open
Infectious proteins or prions are a remarkable class of pathogens, where pathogenicity and infectious state correspond to conformational transition of a protein fold. The conformational change translates into the formation by the protein o…
View article: Protein resonance assignment by solid-state NMR based on <sup>1</sup>H-detected <sup>13</sup>C-based double-quantum spectroscopy at fast MAS
Protein resonance assignment by solid-state NMR based on <sup>1</sup>H-detected <sup>13</sup>C-based double-quantum spectroscopy at fast MAS Open
Solid-state NMR spectroscopy is a powerful technique to study insoluble and non-crystalline proteins and protein complexes at atomic resolution. The development of proton ( 1 H) detection at fast magic-angle spinning (MAS) has considerably…
View article: Structural and molecular basis of cross-seeding barriers in amyloids
Structural and molecular basis of cross-seeding barriers in amyloids Open
Significance Amyloid folds, while performing functional roles in most domains of life, remain a key factor in the emergence and development of multiple neurodegenerative disorders in humans. The significance of our study is twofold: first,…