Melanie L. Hart
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View article: Author Correction: Rethinking the scientific method in the age of AI
Author Correction: Rethinking the scientific method in the age of AI Open
View article: Advancing biomimetic bone Scaffolds: From electrospun 2D membranes to functional 3D nanofiber constructs
Advancing biomimetic bone Scaffolds: From electrospun 2D membranes to functional 3D nanofiber constructs Open
View article: Serum cytokines in primary new daily persistent headache and chronic migraine: a case control study
Serum cytokines in primary new daily persistent headache and chronic migraine: a case control study Open
Background New daily persistent headache (NDPH) is a poorly understood headache disorder which often begins after a viral infection and has been hypothesised to have an immune basis. Several studies have found differences in peripheral ser…
View article: 2024 VCP International Conference: Exploring multi - disciplinary approaches from basic science of valosin containing protein, an AAA+ ATPase protein , to the therapeutic advancement for VCP - associated multisystem proteinopathy
2024 VCP International Conference: Exploring multi - disciplinary approaches from basic science of valosin containing protein, an AAA+ ATPase protein , to the therapeutic advancement for VCP - associated multisystem proteinopathy Open
Valosin-containing protein (VCP/p97) is a ubiquitously expressed AAA+ ATPase associated with numerous protein-protein interactions and critical cellular functions including protein degradation and clearance, mitochondrial homeostasis, DNA …
View article: Role of physical activity and sedentary behavior in venous thromboembolism: a systematic review and dose-response meta-analysis
Role of physical activity and sedentary behavior in venous thromboembolism: a systematic review and dose-response meta-analysis Open
Increasing studies have investigated the link between physical activity (PA) and sedentary behavior with venous thromboembolism (VTE) but the existing findings are not consistent and the independent relationship is uncertain. This meta-ana…
View article: Editorial: Artificial intelligence in predicting, determining and controlling cell phenotype or tissue function in inflammatory diseases
Editorial: Artificial intelligence in predicting, determining and controlling cell phenotype or tissue function in inflammatory diseases Open
Editorial: Artificial intelligence in predicting, determining and controlling cell phenotype or tissue function in inflammatory diseases
View article: Prediction of six macrophage phenotypes and their IL-10 content based on single-cell morphology using artificial intelligence
Prediction of six macrophage phenotypes and their IL-10 content based on single-cell morphology using artificial intelligence Open
Introduction The last decade has led to rapid developments and increased usage of computational tools at the single-cell level. However, our knowledge remains limited in how extracellular cues alter quantitative macrophage morphology and h…
View article: Effect of Vancomycin, Gentamicin and Clindamycin on Cartilage Cells In Vitro
Effect of Vancomycin, Gentamicin and Clindamycin on Cartilage Cells In Vitro Open
Background: The treatment of grafts with vancomycin for ligament reconstruction in knee surgery is the current standard. However, high antibiotic concentrations have chondrotoxic effects. Purpose: To test the chondrotoxicity of clindamycin…
View article: Articular Cartilage—From Basic Science Structural Imaging to Non-Invasive Clinical Quantitative Molecular Functional Information for AI Classification and Prediction
Articular Cartilage—From Basic Science Structural Imaging to Non-Invasive Clinical Quantitative Molecular Functional Information for AI Classification and Prediction Open
This review presents the changes that the imaging of articular cartilage has undergone throughout the last decades. It highlights that the expectation is no longer to image the structure and associated functions of articular cartilage but,…
View article: Therapeutic Modulation of Cell Morphology and Phenotype of Diseased Human Cells towards a Healthier Cell State Using Lignin
Therapeutic Modulation of Cell Morphology and Phenotype of Diseased Human Cells towards a Healthier Cell State Using Lignin Open
Despite lignin’s global abundance and its use in biomedical studies, our understanding of how lignin regulates disease through modulation of cell morphology and associated phenotype of human cells is unknown. We combined an automated high-…
View article: Cell morphology as a biological fingerprint of chondrocyte phenotype in control and inflammatory conditions
Cell morphology as a biological fingerprint of chondrocyte phenotype in control and inflammatory conditions Open
Introduction Little is known how inflammatory processes quantitatively affect chondrocyte morphology and how single cell morphometric data could be used as a biological fingerprint of phenotype. Methods We investigated whether trainable hi…
View article: Mechanical Articular Cartilage Injury Models and Their Relevance in Advancing Therapeutic Strategies
Mechanical Articular Cartilage Injury Models and Their Relevance in Advancing Therapeutic Strategies Open
This chapter details how Alan Grodzinsky and his team unraveled the complex electromechanobiological structure-function relationships of articular cartilage and used these insights to develop an impressively versatile shear and compression…
View article: Characterization and In Vitro Cytotoxicity Safety Screening of Fractionated Organosolv Lignin on Diverse Primary Human Cell Types Commonly Used in Tissue Engineering
Characterization and In Vitro Cytotoxicity Safety Screening of Fractionated Organosolv Lignin on Diverse Primary Human Cell Types Commonly Used in Tissue Engineering Open
There is limited data assessing the cytotoxic effects of organosolv lignin with cells commonly used in tissue engineering. Structural and physico-chemical characterization of fractionated organosolv lignin showed that a decrease of the mol…
View article: ABSTRACTS (BY NUMBER)
ABSTRACTS (BY NUMBER) Open
Chronic Obstructive Pulmonary Disease (COPD) presents a significant, incurable, worldwide health burden, identifying it as a prospective regenerative medicine target. Sourcing cells for lung therapies is challenging; progress has been made…
View article: Controlled Growth Factor Delivery and Cyclic Stretch Induces a Smooth Muscle Cell-like Phenotype in Adipose-Derived Stem Cells
Controlled Growth Factor Delivery and Cyclic Stretch Induces a Smooth Muscle Cell-like Phenotype in Adipose-Derived Stem Cells Open
Adipose-derived stem cells (ASCs) are an abundant and easily accessible multipotent stem cell source with potential application in smooth muscle regeneration strategies. In 3D collagen hydrogels, we investigated whether sustained release o…
View article: Anti-Inflammatory Therapeutic Approaches to Prevent or Delay Post-Traumatic Osteoarthritis (PTOA) of the Knee Joint with a Focus on Sustained Delivery Approaches
Anti-Inflammatory Therapeutic Approaches to Prevent or Delay Post-Traumatic Osteoarthritis (PTOA) of the Knee Joint with a Focus on Sustained Delivery Approaches Open
Inflammation plays a central role in the pathogenesis of knee PTOA after knee trauma. While a comprehensive therapy capable of preventing or delaying post-traumatic osteoarthritis (PTOA) progression after knee joint injury does not yet cli…
View article: Articular Chondrocyte Phenotype Regulation through the Cytoskeleton and the Signaling Processes That Originate from or Converge on the Cytoskeleton: Towards a Novel Understanding of the Intersection between Actin Dynamics and Chondrogenic Function
Articular Chondrocyte Phenotype Regulation through the Cytoskeleton and the Signaling Processes That Originate from or Converge on the Cytoskeleton: Towards a Novel Understanding of the Intersection between Actin Dynamics and Chondrogenic Function Open
Numerous studies have assembled a complex picture, in which extracellular stimuli and intracellular signaling pathways modulate the chondrocyte phenotype. Because many diseases are mechanobiology-related, this review asked to what extent p…
View article: An Evidence-Based Systematic Review of Human Knee Post-Traumatic Osteoarthritis (PTOA): Timeline of Clinical Presentation and Disease Markers, Comparison of Knee Joint PTOA Models and Early Disease Implications
An Evidence-Based Systematic Review of Human Knee Post-Traumatic Osteoarthritis (PTOA): Timeline of Clinical Presentation and Disease Markers, Comparison of Knee Joint PTOA Models and Early Disease Implications Open
Understanding the causality of the post-traumatic osteoarthritis (PTOA) disease process of the knee joint is important for diagnosing early disease and developing new and effective preventions or treatments. The aim of this review was to p…
View article: Mechanotransduction and Stiffness-Sensing: Mechanisms and Opportunities to Control Multiple Molecular Aspects of Cell Phenotype as a Design Cornerstone of Cell-Instructive Biomaterials for Articular Cartilage Repair
Mechanotransduction and Stiffness-Sensing: Mechanisms and Opportunities to Control Multiple Molecular Aspects of Cell Phenotype as a Design Cornerstone of Cell-Instructive Biomaterials for Articular Cartilage Repair Open
Since material stiffness controls many cell functions, we reviewed the currently available knowledge on stiffness sensing and elucidated what is known in the context of clinical and experimental articular cartilage (AC) repair. Remarkably,…
View article: Lack of a skeletal muscle phenotype in adult human bone marrow stromal cells following xenogeneic-free expansion
Lack of a skeletal muscle phenotype in adult human bone marrow stromal cells following xenogeneic-free expansion Open
View article: Bioresponsive microspheres for on‐demand delivery of anti‐inflammatory cytokines for articular cartilage repair
Bioresponsive microspheres for on‐demand delivery of anti‐inflammatory cytokines for articular cartilage repair Open
Despite innovations in surgical interventions, treatment of cartilage injury in osteoarthritic joints remains a challenge due to concomitant inflammation. Obstructing a single dominant inflammatory cytokine has shown remarkable clinical be…
View article: Bioresponsive Microspheres for On-demand Delivery of Anti-inflammatory Cytokines for Osteoarthritis
Bioresponsive Microspheres for On-demand Delivery of Anti-inflammatory Cytokines for Osteoarthritis Open
Despite innovations in surgical interventions, treatment of cartilage injury in osteoarthritic joints remains a challenge due to concomitant inflammation. Obstructing a single dominant inflammatory cytokine have shown remarkable clinical b…
View article: Shaping the Cell and the Future: Recent Advancements in Biophysical Aspects Relevant to Regenerative Medicine
Shaping the Cell and the Future: Recent Advancements in Biophysical Aspects Relevant to Regenerative Medicine Open
In a worldwide effort to generate clinically useful therapeutic or preventive interventions, harnessing biophysical stimuli for directing cell fate is a powerful strategy. With the vision to control cell function through engineering cell s…
View article: Retraction: Hypoxia-Inducible Factor-1α–Dependent Protection from Intestinal Ischemia/Reperfusion Injury Involves Ecto-5′-Nucleotidase (CD73) and the A2B Adenosine Receptor
Retraction: Hypoxia-Inducible Factor-1α–Dependent Protection from Intestinal Ischemia/Reperfusion Injury Involves Ecto-5′-Nucleotidase (CD73) and the A2B Adenosine Receptor Open
Intestinal ischemia/reperfusion injury (IR) is characterized by intermittent loss of perfusion to the gut, resulting in dramatic increases in morbidity and mortality. Based on previous studies indicating an anti-inflammatory role for hypox…
View article: Engineering the geometrical shape of mesenchymal stromal cells through defined cyclic stretch regimens
Engineering the geometrical shape of mesenchymal stromal cells through defined cyclic stretch regimens Open
View article: The geometrical shape of mesenchymal stromal cells measured by quantitative shape descriptors is determined by the stiffness of the biomaterial and by cyclic tensile forces
The geometrical shape of mesenchymal stromal cells measured by quantitative shape descriptors is determined by the stiffness of the biomaterial and by cyclic tensile forces Open
Controlling mesenchymal stromal cell (MSC) shape is a novel method for investigating and directing MSC behaviour in vitro. it was hypothesized that specifigc MSC shapes can be generated by using stiffness-defined biomaterial surfaces and b…
View article: Stretching human mesenchymal stromal cells on stiffness-customized collagen type I generates a smooth muscle marker profile without growth factor addition
Stretching human mesenchymal stromal cells on stiffness-customized collagen type I generates a smooth muscle marker profile without growth factor addition Open
Using matrix elasticity and cyclic stretch have been investigated for inducing mesenchymal stromal cell (MSC) differentiation towards the smooth muscle cell (SMC) lineage but not in combination. We hypothesized that combining lineage-speci…
View article: Loss of spatial organization and destruction of the pericellular matrix in early osteoarthritis in vivo and in a novel in vitro methodology
Loss of spatial organization and destruction of the pericellular matrix in early osteoarthritis in vivo and in a novel in vitro methodology Open
View article: Loss of spatial organization and destruction of the pericellular matrix in early osteoarthritis in vivo and in a novel in vitro methodology
Loss of spatial organization and destruction of the pericellular matrix in early osteoarthritis in vivo and in a novel in vitro methodology Open
View article: Bone marrow-derived mesenchymal stromal cells differ in their attachment to fibronectin-derived peptides from term placenta-derived mesenchymal stromal cells
Bone marrow-derived mesenchymal stromal cells differ in their attachment to fibronectin-derived peptides from term placenta-derived mesenchymal stromal cells Open