Melissa N. Locke
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View article: RECON gene disruption enhances host resistance to enable genome-wide evaluation of intracellular pathogen fitness during infection
RECON gene disruption enhances host resistance to enable genome-wide evaluation of intracellular pathogen fitness during infection Open
Transposon sequencing (Tn-seq) is a powerful genome-wide technique to assess bacterial fitness under varying growth conditions. However, screening via Tn-seq in vivo is challenging. Dose limitations and host restrictions create bottlenecks…
View article: RECON gene disruption enhances host resistance to enable genome-wide evaluation of intracellular pathogen fitness during infection
RECON gene disruption enhances host resistance to enable genome-wide evaluation of intracellular pathogen fitness during infection Open
Transposon sequencing (Tn-seq) is a powerful genome-wide technique to assess bacterial fitness under varying growth conditions. However, screening via Tn-seq in vivo is challenging. Dose limitations and host restrictions create bottlenecks…
View article: Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair
Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair Open
View article: Regulation of TORC2 function and localization by Rab5 GTPases in<i>Saccharomyces cerevisiae</i>
Regulation of TORC2 function and localization by Rab5 GTPases in<i>Saccharomyces cerevisiae</i> Open
The evolutionarily conserved Target of Rapamycin (TOR) complex-2 (TORC2) is an essential regulator of plasma membrane homeostasis in budding yeast (Saccharomyces cerevisiae). In this yeast, TORC2 phosphorylates and activates the eff…
View article: Molecular Mechanisms in Signaling by Target of Rapamycin Complex 2 in Saccharomyces cerevisiae
Molecular Mechanisms in Signaling by Target of Rapamycin Complex 2 in Saccharomyces cerevisiae Open
Protein kinases are enzymes that transfer the γ-phosphate of ATP to acceptor residues, most often serine, threonine or tyrosine, in proteins. Phosphorylation by protein kinases are integral to the signaling processes that control virtually…
View article: Rab5 GTPases are required for optimal TORC2 function
Rab5 GTPases are required for optimal TORC2 function Open
Target of rapamycin complex-2 (TORC2), a conserved protein kinase complex, is an indispensable regulator of plasma membrane homeostasis. In budding yeast (Saccharomyces cerevisiae), the essential downstream effector of TORC2 is protein kin…
View article: Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair
Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair Open
Repair of double strand DNA breaks (DSBs) can result in gene disruption or precise gene modification via homology directed repair (HDR) from a templating donor DNA. During genome editing, altering cellular responses to DSBs may be an effec…
View article: The TORC2‐Dependent Signaling Network in the Yeast Saccharomyces cerevisiae
The TORC2‐Dependent Signaling Network in the Yeast Saccharomyces cerevisiae Open
To grow, eukaryotic cells must expand by inserting glycerolipids, sphingolipids, sterols, and proteins into their plasma membrane, and maintain the proper levels and bilayer distribution. A fungal cell must coordinate growth with enlargeme…
View article: A Conserved Deubiquitinating Enzyme Uses Intrinsically Disordered Regions to Scaffold Multiple Protein Interaction Sites
A Conserved Deubiquitinating Enzyme Uses Intrinsically Disordered Regions to Scaffold Multiple Protein Interaction Sites Open
In the canonical view of protein function, it is generally accepted that the three-dimensional structure of a protein determines its function. However, the past decade has seen a dramatic growth in the identification of proteins with exten…