Manuel Castro
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View article: The synaptic architecture of layer 5 thick tufted excitatory neurons in mouse visual cortex
The synaptic architecture of layer 5 thick tufted excitatory neurons in mouse visual cortex Open
Despite significant progress in characterizing neocortical cell types, a complete understanding of the synaptic connections of individual excitatory cells remains elusive. This study investigates the connectivity of mouse visual cortex thi…
View article: SHS Graduates' Employment: Curriculum Impact and Challenges
SHS Graduates' Employment: Curriculum Impact and Challenges Open
View article: Enhancing graph interpretation skills of Grade 9 students in learning microeconomics through I2 strategy
Enhancing graph interpretation skills of Grade 9 students in learning microeconomics through I2 strategy Open
This action research aimed to enhance the graph interpretation skills of 35 Grade 9 Apitong students of Baybay National High School in learning microeconomics through the I2 strategy. Utilizing a mixed-methods research design, the study in…
View article: A Importância da Formação Continuada para Gestores Escolares
A Importância da Formação Continuada para Gestores Escolares Open
Introdução: O aprimoramento da liderança e da gestão educacional depende do investimento no processo de formação continuada dos gestores escolares, com vista no fortalecimento da qualidade da educação e a eficiência na tomada de decisões. …
View article: Connectomics of predicted Sst transcriptomic types in mouse visual cortex
Connectomics of predicted Sst transcriptomic types in mouse visual cortex Open
Neural circuit function is shaped both by the cell types that comprise the circuit and the connections between them1. Neural cell types have previously been defined by morphology2,3, electrophysiology4, tra…
View article: Perisomatic ultrastructure efficiently classifies cells in mouse cortex
Perisomatic ultrastructure efficiently classifies cells in mouse cortex Open
View article: An unsupervised map of excitatory neuron dendritic morphology in the mouse visual cortex
An unsupervised map of excitatory neuron dendritic morphology in the mouse visual cortex Open
Neurons in the neocortex exhibit astonishing morphological diversity, which is critical for properly wiring neural circuits and giving neurons their functional properties. However, the organizational principles underlying this morphologica…
View article: Functional connectomics spanning multiple areas of mouse visual cortex
Functional connectomics spanning multiple areas of mouse visual cortex Open
Understanding the brain requires understanding neurons’ functional responses to the circuit architecture shaping them. Here we introduce the MICrONS functional connectomics dataset with dense calcium imaging of around 75,000 neurons in pri…
View article: NEURD offers automated proofreading and feature extraction for connectomics
NEURD offers automated proofreading and feature extraction for connectomics Open
We are in the era of millimetre-scale electron microscopy volumes collected at nanometre resolution 1,2 . Dense reconstruction of cellular compartments in these electron microscopy volumes has been enabled by recent advances in machine lea…
View article: Dificuldades e Transtorno de Aprendizagem no Ensino Fundamental: Desafios e Perspectivas
Dificuldades e Transtorno de Aprendizagem no Ensino Fundamental: Desafios e Perspectivas Open
Introdução: A escola é um dos principais cenários onde se manifestam, de forma clara e explícita, as dificuldades de aprendizagem, que são identificadas quando o estudante não alcança o mínimo de aprendizado esperado, ou não consegue assim…
View article: The Synaptic Architecture of Layer 5 Thick Tufted Excitatory Neurons in the Visual Cortex of Mice
The Synaptic Architecture of Layer 5 Thick Tufted Excitatory Neurons in the Visual Cortex of Mice Open
The neocortex is composed of microcircuits built from distinct cell types. Despite significant progress in characterizing these cell types, understanding the full synaptic connections of individual excitatory cells remains elusive. This st…
View article: Connectomic reconstruction of a female Drosophila ventral nerve cord
Connectomic reconstruction of a female Drosophila ventral nerve cord Open
View article: Optimizing NMR fragment-based drug screening for membrane protein targets
Optimizing NMR fragment-based drug screening for membrane protein targets Open
NMR spectroscopy has played a pivotal role in fragment-based drug discovery by coupling detection of weak ligand-target binding with structural mapping of the binding site. Fragment-based screening by NMR has been successfully applied to m…
View article: The Synaptic Architecture of Layer 5 Thick Tufted Excitatory Neurons in the Visual Cortex of Mice
The Synaptic Architecture of Layer 5 Thick Tufted Excitatory Neurons in the Visual Cortex of Mice Open
Summary The neocortex is one of the most critical structures that makes us human, and it is involved in a variety of cognitive functions from perception to sensory integration and motor control. Composed of repeated modules, or microcircui…
View article: Functional connectomics reveals general wiring rule in mouse visual cortex
Functional connectomics reveals general wiring rule in mouse visual cortex Open
Understanding the relationship between circuit connectivity and function is crucial for uncovering how the brain implements computation. In the mouse primary visual cortex (V1), excitatory neurons with similar response properties are more …
View article: Origins of glycan selectivity in streptococcal Siglec-like adhesins suggest mechanisms of receptor adaptation
Origins of glycan selectivity in streptococcal Siglec-like adhesins suggest mechanisms of receptor adaptation Open
View article: Petascale pipeline for precise alignment of images from serial section electron microscopy
Petascale pipeline for precise alignment of images from serial section electron microscopy Open
The reconstruction of neural circuits from serial section electron microscopy (ssEM) images is being accelerated by automatic image segmentation methods. Segmentation accuracy is often limited by the preceding step of aligning 2D section i…
View article: Verteporfin is a substrate-selective γ-secretase inhibitor that binds the amyloid precursor protein transmembrane domain
Verteporfin is a substrate-selective γ-secretase inhibitor that binds the amyloid precursor protein transmembrane domain Open
This work reports substrate-selective inhibition of a protease with broad substrate specificity based on direct binding of a small-molecule inhibitor to the substrate. The target for these studies was γ-secretase protease, which cleaves do…
View article: A Model for the Signal Initiation Complex Between Arrestin-3 and the Src Family Kinase Fgr
A Model for the Signal Initiation Complex Between Arrestin-3 and the Src Family Kinase Fgr Open
View article: Labore in der Hochschullehre
Labore in der Hochschullehre Open
In der Hochschullehre ist das Labor als Raum des praktischen Lehrens und Lernens in den technischen Fächern ein zentraler Bestandteil der Curricula. Damit der "Lernort Labor" einen positiven Beitrag zum Kompetenzerwerb der Lernenden leiste…
View article: Ubiquitous and Smart Learning Paradigm for Preparing Qualified and Skilled Engineers
Ubiquitous and Smart Learning Paradigm for Preparing Qualified and Skilled Engineers Open
Ubiquitous and Smart Learning Paradigm for Preparing Qualified and Skilled EngineersAbstractUnskilled and non-qualified graduates are considered to be two of the most common threatsthat have been associated with most of the official engine…
View article: Evidence that the TRPV1 S1-S4 membrane domain contributes to thermosensing
Evidence that the TRPV1 S1-S4 membrane domain contributes to thermosensing Open
Sensing and responding to temperature is crucial in biology. The TRPV1 ion channel is a well-studied heat-sensing receptor that is also activated by vanilloid compounds, including capsaicin. Despite significant interest, the molecular unde…
View article: Chandelier cell anatomy and function reveal a variably distributed but common signal
Chandelier cell anatomy and function reveal a variably distributed but common signal Open
The activity and connectivity of inhibitory cells has a profound impact on the operation of neuronal networks. While the average connectivity of many inhibitory cell types has been characterized, we still lack an understanding of how indiv…
View article: Exploring the Binding Potency and Specificity of Small Molecules Against the Transmembrane Amyloid Precursor Protein Fragment, C99
Exploring the Binding Potency and Specificity of Small Molecules Against the Transmembrane Amyloid Precursor Protein Fragment, C99 Open
View article: Selectivity and engineering of the sialoglycan-binding spectrum in Siglec-like adhesins
Selectivity and engineering of the sialoglycan-binding spectrum in Siglec-like adhesins Open
The Siglec-like Serine-Rich Repeat (SRR) adhesins mediate bacterial attachment to mammalian hosts via sialoglycan receptors. Here, we combine structural, computational, biochemical, and phylogenetic approaches to elucidate the determinants…
View article: Evidence that the TRPV1 S1-S4 Membrane Domain Contributes to Thermosensing
Evidence that the TRPV1 S1-S4 Membrane Domain Contributes to Thermosensing Open
Sensing and responding to temperature is crucial in biology. The TRPV1 ion channel is a well-studied heat-sensing receptor that is also activated by vanilloid compounds including capsaicin. Despite significant interest, the molecular under…
View article: The vexing complexity of the amyloidogenic pathway
The vexing complexity of the amyloidogenic pathway Open
The role of the amyloidogenic pathway in the etiology of Alzheimer's disease (AD), particularly the common sporadic late onset forms of the disease, is controversial. To some degree, this is a consequence of the failure of drug and therape…
View article: Table of contents
Table of contents Open
View article: Contributors
Contributors Open
View article: USING A 3-TIER TRAINING MODEL FOR EFFECTIVE EXCHANGE OF GOOD PRACTICES IN AN ERASMUS+ PROJECT
USING A 3-TIER TRAINING MODEL FOR EFFECTIVE EXCHANGE OF GOOD PRACTICES IN AN ERASMUS+ PROJECT Open
VISIR+ is an Erasmus+ project that aims to develop educational modules for electric and electronic circuits theory and practice following an enquiry-based teaching and learning methodology. The project has installed five new VISIR remote l…