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View article: Placental infection by SARS-CoV-2: exploring alternative entry pathways
Placental infection by SARS-CoV-2: exploring alternative entry pathways Open
The placenta possesses several structural and immunological barriers against viral infections, the SARS-CoV-2 detection in placental tissues has raised concerns regarding possible alternative viral entry mechanisms beyond the canonical ACE…
View article: Assessment of the relationship between hematologic parameters, (CPD), in screening for COVID-19 severity in women
Assessment of the relationship between hematologic parameters, (CPD), in screening for COVID-19 severity in women Open
Hematological parameters, particularly CPDs and NLR, are valuable for early stratification of COVID-19 severity. These automated, rapid, and cost-effective measures can support clinical decision-making. However, CPD usage depends on analyz…
View article: Assessing the impact of the Gamma variant on COVID-19 patient admissions in a southern Brazilian tertiary hospital—A comparison of dual pandemic phases
Assessing the impact of the Gamma variant on COVID-19 patient admissions in a southern Brazilian tertiary hospital—A comparison of dual pandemic phases Open
Since the first case of COVID-19, Brazil has undergone infection waves with distinct characteristics. The description of new variants has alerted the emergence of more contagious or virulent viruses. The variant of concern Gamma emerged in…
View article: INTERNAMENTOS POR POR COVID-19 EM DOIS DIFERENTES MOMENTOS DA PANDEMIA EM UM HOSPITAL TERCIÁRIO NO SUL DO BRASIL: IMPACTO DA VARIANTE GAMMA
INTERNAMENTOS POR POR COVID-19 EM DOIS DIFERENTES MOMENTOS DA PANDEMIA EM UM HOSPITAL TERCIÁRIO NO SUL DO BRASIL: IMPACTO DA VARIANTE GAMMA Open
Introdução: A pandemia causada pelo SARS-CoV-2 atingiu virtualmente todos os países do mundo. No Brasil, devido à diversidade regional e socioeconômica, houve diferenças na evolução dos casos e óbitos nas diferentes regiões. O surgimento d…
View article: Assessing the impact of the Gamma variant on COVID-19 Patient admissions in a Southern Brazilian tertiary hospital - A comparison of dual pandemic phases
Assessing the impact of the Gamma variant on COVID-19 Patient admissions in a Southern Brazilian tertiary hospital - A comparison of dual pandemic phases Open
Since the first case of COVID-19, Brazil has undergone infection waves with distinct characteristics. The description of new variants has alerted the emergence of more contagious or virulent viruses. The variant of concern Gamma emerged in…
View article: IgA antibody dynamics in healthcare workers after CoronaVac® vaccination and heterologous Comirnaty® booster dose
IgA antibody dynamics in healthcare workers after CoronaVac® vaccination and heterologous Comirnaty® booster dose Open
View article: SARS-CoV-2 Delta and Omicron Variants Surge in Curitiba, Southern Brazil, and Its Impact on Overall COVID-19 Lethality
SARS-CoV-2 Delta and Omicron Variants Surge in Curitiba, Southern Brazil, and Its Impact on Overall COVID-19 Lethality Open
Screening efforts and genomic surveillance are essential tools to evaluate the course of the COVID-19 pandemic and assist the public healthcare system in dealing with an increasing number of infections. For the analysis of COVID-19 cases s…
View article: Comparison of SARS-CoV-2 molecular detection in nasopharyngeal swab, saliva, and gargle samples
Comparison of SARS-CoV-2 molecular detection in nasopharyngeal swab, saliva, and gargle samples Open
View article: Large-Scale Screening of Asymptomatic Persons for SARS-CoV-2 Variants of Concern and Gamma Takeover, Brazil
Large-Scale Screening of Asymptomatic Persons for SARS-CoV-2 Variants of Concern and Gamma Takeover, Brazil Open
We performed a large-scale severe acute respiratory syndrome coronavirus 2 screening campaign using 2 PCR-based approaches, coupled with variant genotyping, aiming to provide a safer environment for employees of Federal University in Curit…
View article: Antigen production and development of an indirect ELISA based on the nucleocapsid protein to detect human SARS-CoV-2 seroconversion
Antigen production and development of an indirect ELISA based on the nucleocapsid protein to detect human SARS-CoV-2 seroconversion Open
Serological assays are important tools to identify previous exposure to SARS-CoV-2, helping to track COVID-19 cases and determine the level of humoral response to SARS-CoV-2 infections and/or immunization to future vaccines. Here, the SARS…
View article: SARS-CoV-2 in asymptomatic pregnant women in South Brazil: RT-PCR and serological detection
SARS-CoV-2 in asymptomatic pregnant women in South Brazil: RT-PCR and serological detection Open
Objectives This study aims to detect the SARS-CoV-2 infection prevalence in asymptomatic pregnant women. Methods A group of 195 asymptomatic pregnant women who attended the prenatal care outclinic and to the obstetric emergency department …
View article: Association Between COVID-19 Pregnant Women Symptoms Severity and Placental Morphologic Features
Association Between COVID-19 Pregnant Women Symptoms Severity and Placental Morphologic Features Open
Since the beginning of the pandemic, few papers describe the placenta’s morphological and morphometrical features in SARS-CoV-2–positive pregnant women. Alterations, such as low placental weight, accelerated villous maturation, decidual va…
View article: Antigen production and development of an indirect ELISA based on the nucleocapsid protein to detect human SARS-CoV-2 seroconversion
Antigen production and development of an indirect ELISA based on the nucleocapsid protein to detect human SARS-CoV-2 seroconversion Open
Serological assays are important tools to identify previous exposure to SARS-CoV-2, helping to track COVID-19 cases and determine the level of humoral response to SARS-CoV-2 infections and/or immunization to future vaccines. Here the SARS-…
View article: Magnetic Bead-Based Immunoassay Allows Rapid, Inexpensive, and Quantitative Detection of Human SARS-CoV-2 Antibodies
Magnetic Bead-Based Immunoassay Allows Rapid, Inexpensive, and Quantitative Detection of Human SARS-CoV-2 Antibodies Open
Immunological methods to detect SARS-CoV-2 seroconversion in humans are important to track COVID-19 cases and the humoral response to SARS-CoV-2 infections and immunization to future vaccines. The aim of this work was to develop a simple c…
View article: SARS-CoV-2 Vaccination with CoronaVac: Seroconversion Rate in Healthcare Workers After 40 Days
SARS-CoV-2 Vaccination with CoronaVac: Seroconversion Rate in Healthcare Workers After 40 Days Open
View article: Rapid Serological Tests for SARS-CoV-2: Diagnostic Performance of 4 Commercial Assays
Rapid Serological Tests for SARS-CoV-2: Diagnostic Performance of 4 Commercial Assays Open
Objective: To assess the diagnostic performance of lateral flow immunochromatographic assays (LFAs) of 4 different manufacturers to identify SARS-CoV-2 antibodies (IgM, IgG, or total), comparing them with the nucleic acid amp…
View article: Rapid Serological Tests for SARS-COV-2: Diagnostic Performance of Four Commercial Assays
Rapid Serological Tests for SARS-COV-2: Diagnostic Performance of Four Commercial Assays Open
Background. This study aimed to assess the diagnostic performance of lateral flow immunochromatographic assays (LFA) of four different manufacturers to identify SARS-CoV-2 antibodies (IgM, IgG or total), comparing them with the nucleic aci…
View article: Intrauterine Transmission of SARS-CoV-2
Intrauterine Transmission of SARS-CoV-2 Open
We documented fetal death associated with intrauterine transmission of severe acute respiratory syndrome coronavirus 2. We found chronic histiocytic intervillositis, maternal and fetal vascular malperfusion, microglial hyperplasia, and lym…
View article: Rapid Serological Tests for SARS-COV-2: Diagnostic Performance of Four Commercial Assays
Rapid Serological Tests for SARS-COV-2: Diagnostic Performance of Four Commercial Assays Open
Background. This study aimed to assess the diagnostic performance of lateral flow immunochromatographic assays (LFA) of four different manufacturers to identify SARS-CoV-2 antibodies (IgM, IgG or total), comparing them with the nucleic aci…
View article: Magnetic bead-based ELISA allow inexpensive, rapid and quantitative detection of human antibodies against SARS-CoV-2
Magnetic bead-based ELISA allow inexpensive, rapid and quantitative detection of human antibodies against SARS-CoV-2 Open
Here we describe a novel immunogenic method to detect COVID-19. The method is a chromogenic magnetic bead-based ELISA which allows inexpensive and quantitative detection of human IgG or IgM antibodies against SARS-CoV-2 in serum or whole b…
View article: Influenza Sentinel Surveillance and Severe Acute Respiratory Infection in a Reference Hospital in Southern Brazil
Influenza Sentinel Surveillance and Severe Acute Respiratory Infection in a Reference Hospital in Southern Brazil Open
In the post-epidemic period, influenza remains an important cause of hospitalization in SARI patients.
View article: Comparison of Cerebrospinal Fluid Biomarkers for Differential Diagnosis of Acute Bacterial and Viral Meningitis with Atypical Cerebrospinal Fluid Characteristics
Comparison of Cerebrospinal Fluid Biomarkers for Differential Diagnosis of Acute Bacterial and Viral Meningitis with Atypical Cerebrospinal Fluid Characteristics Open
Objective: Several cerebrospinal fluid (CSF) biomarkers are used to distinguish between acute bacterial meningitis (BM) and viral meningitis (VM). We compared the ability of lactate and glucose (GL) in CSF and the CSF/blood G…
View article: Influenza Sentinel Surveillance and Severe Acute Respiratory Infection in a Reference Hospital in Southern Brazil
Influenza Sentinel Surveillance and Severe Acute Respiratory Infection in a Reference Hospital in Southern Brazil Open
INTRODUCTION: We report the results of the active surveillance of influenza infections in hospitalized patients and the evaluation of the seasonality and correlation with temperature and rainfall data. METHODS: During the 2-year study peri…
View article: The influence of temperature and rainfall in the human viral infections
The influence of temperature and rainfall in the human viral infections Open
It is well described by many authors the occurrence of viruses outbreaks that occurs annually at the same time. Epidemiological studies could generate important information about seasonality and its relation to outbreaks, population fluctu…
View article: Global Influenza Hospital-based Surveillance Network (GIHSN): results of surveillance of influenza and other respiratory viruses in hospitalised patients in Brazil, 2015
Global Influenza Hospital-based Surveillance Network (GIHSN): results of surveillance of influenza and other respiratory viruses in hospitalised patients in Brazil, 2015 Open
Background Influenza-like illness occurs annually worldwide, with peak timing and severity varying seasonally, resulting in significant annual mortality. Objectives There were three objectives: (1) to describe the epidemiological and clini…
View article: Profile of HIV subtypes in HIV/HBV- and HIV/HCV-coinfected patients in Southern Brazil
Profile of HIV subtypes in HIV/HBV- and HIV/HCV-coinfected patients in Southern Brazil Open
In the present case series, a higher frequency of HIV subtype C was found in coinfected patients. However such findings need to be prospectively evaluated with the inclusion of data from regional multicenter analyses.
View article: Human parechovirus: sepsis-like illness with pulmonary infection
Human parechovirus: sepsis-like illness with pulmonary infection Open
View article: Impact and seasonality of human rhinovirus infection in hospitalized patients for two consecutive years
Impact and seasonality of human rhinovirus infection in hospitalized patients for two consecutive years Open
View article: Impact and seasonality of human rhinovirus infection in hospitalized patients for two consecutive years
Impact and seasonality of human rhinovirus infection in hospitalized patients for two consecutive years Open
HRV is usually detected in hospitalized children with respiratory infections and is often present in viral co-detections. Comorbidities are closely associated with HRV infections. These infections show seasonal variation, with predominance…
View article: Red blood cells in cerebrospinal fluid as possible inhibitory factor for enterovirus RT-PCR
Red blood cells in cerebrospinal fluid as possible inhibitory factor for enterovirus RT-PCR Open
The presence of hemoglobin in samples are considered an important inhibitory factor for polymerase chain reaction (PCR). The aim of this study was to examine the influence of red blood cells (RBC)s in cerebrospinal fluid (CSF) as an inhibi…