Micah Rapp
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View article: Better, Faster, Cheaper: Recent Advances in Cryo–Electron Microscopy
Better, Faster, Cheaper: Recent Advances in Cryo–Electron Microscopy Open
Cryo–electron microscopy (cryo-EM) continues its remarkable growth as a method for visualizing biological objects, which has been driven by advances across the entire pipeline. Developments in both single-particle analysis and in situ tomo…
View article: Visualizing cadherin intermembrane adhesion assemblies using cryo-electron tomography
Visualizing cadherin intermembrane adhesion assemblies using cryo-electron tomography Open
An abstract is not available for this content so a preview has been provided. As you have access to this content, a full PDF is available via the ‘Save PDF’ action button.
View article: Modular basis for potent SARS-CoV-2 neutralization by a prevalent VH1-2-derived antibody class
Modular basis for potent SARS-CoV-2 neutralization by a prevalent VH1-2-derived antibody class Open
Antibodies with heavy chains that derive from the VH1-2 gene constitute some of the most potent severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-neutralizing antibodies yet identified. To provide insight into whether these gene…
View article: Structural Basis for Accommodation of Emerging B.1.351 and B.1.1.7 Variants by Two Potent SARS-CoV-2 Neutralizing Antibodies
Structural Basis for Accommodation of Emerging B.1.351 and B.1.1.7 Variants by Two Potent SARS-CoV-2 Neutralizing Antibodies Open
Summary Emerging SARS-CoV-2 strains, B.1.1.7 and B.1.351, from the UK and South Africa, respectively show decreased neutralization by monoclonal antibodies and convalescent or vaccinee sera raised against the original wild-type virus, and …
View article: Modular basis for potent SARS-CoV-2 neutralization by a prevalent VH1-2-derived antibody class
Modular basis for potent SARS-CoV-2 neutralization by a prevalent VH1-2-derived antibody class Open
SUMMARY Antibodies with heavy chains that derive from the VH1-2 gene constitute some of the most potent SARS-CoV-2-neutralizing antibodies yet identified. To provide insight into whether these genetic similarities inform common modes of re…
View article: Visualizing cell surface interactions using cryogenic electron microscopy
Visualizing cell surface interactions using cryogenic electron microscopy Open
The study of the three-dimensional structures of biological macromolecules has given us significant insight into life and its mechanisms. Understanding these structures in their native contexts, a challenging but important goal, came close…
View article: Cryo-EM Structures Delineate a pH-Dependent Switch that Mediates Endosomal Positioning of SARS-CoV-2 Spike Receptor-Binding Domains
Cryo-EM Structures Delineate a pH-Dependent Switch that Mediates Endosomal Positioning of SARS-CoV-2 Spike Receptor-Binding Domains Open
The SARS-CoV-2 spike employs mobile receptor-binding domains (RBDs) to engage the ACE2 receptor and to facilitate virus entry. Antibodies can engage RBD but some, such as CR3022, fail to inhibit entry despite nanomolar spike affinity. Here…
View article: TOPAZ: A Positive-Unlabeled Convolutional Neural Network CryoEM Particle Picker that can Pick Any Size and Shape Particle
TOPAZ: A Positive-Unlabeled Convolutional Neural Network CryoEM Particle Picker that can Pick Any Size and Shape Particle Open
Journal Article TOPAZ: A Positive-Unlabeled Convolutional Neural Network CryoEM Particle Picker that can Pick Any Size and Shape Particle Get access Tristan Bepler, Tristan Bepler Computational and Systems Biology, MIT, Cambridge, MA, USAC…
View article: CryoET of Single Particle CryoEM Grids Reveals Widespread Particle Adsorption to the Air-Water Interface, Which May be Reduced with New Plunging Techniques
CryoET of Single Particle CryoEM Grids Reveals Widespread Particle Adsorption to the Air-Water Interface, Which May be Reduced with New Plunging Techniques Open
An abstract is not available for this content so a preview has been provided. As you have access to this content, a full PDF is available via the ‘Save PDF’ action button.
View article: Routine single particle CryoEM sample and grid characterization by tomography
Routine single particle CryoEM sample and grid characterization by tomography Open
Single particle cryo-electron microscopy (cryoEM) is often performed under the assumption that particles are not adsorbed to the air-water interfaces and in thin, vitreous ice. In this study, we performed fiducial-less tomography on over 5…
View article: Author response: Routine single particle CryoEM sample and grid characterization by tomography
Author response: Routine single particle CryoEM sample and grid characterization by tomography Open
Article Figures and data Abstract Introduction Results and discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract Single particle cryo-electron microsco…
View article: Routine Single Particle CryoEM Sample and Grid Characterization by Tomography
Routine Single Particle CryoEM Sample and Grid Characterization by Tomography Open
Single particle cryo-electron microscopy (cryoEM) is often performed under the assumption that particles are freely floating away from the air-water interfaces and in thin, vitreous ice. In this study, we performed fiducial-less tomography…