Michael A. Caldwell
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View article: SPAP: Soluble Human Plasma Proteoform Analysis via Acetonitrile Precipitation and Top-Down Mass Spectrometry
SPAP: Soluble Human Plasma Proteoform Analysis via Acetonitrile Precipitation and Top-Down Mass Spectrometry Open
Advances in liquid chromatography-mass spectrometry have significantly improved proteomic analyses of human plasma. However, information at the level of intact proteoforms remains limited due to the high dynamic range of protein abundance …
View article: Next-Generation Protein Sequencing and individual ion mass spectrometry enable complementary analysis of interleukin-6
Next-Generation Protein Sequencing and individual ion mass spectrometry enable complementary analysis of interleukin-6 Open
The vast complexity of the proteome currently overwhelms any single analytical technology in capturing the full spectrum of proteoform diversity. In this study, we evaluated the complementarity of two cutting-edge proteomic technologies—si…
View article: Deep Profiling of Plasma Proteoforms with Engineered Nanoparticles for Top-Down Proteomics
Deep Profiling of Plasma Proteoforms with Engineered Nanoparticles for Top-Down Proteomics Open
The dynamic range challenge for the detection of proteins and their proteoforms in human plasma has been well documented. Here, we use the nanoparticle protein corona approach to enrich low-abundance proteins selectively and reproducibly f…
View article: Deep Profiling of Plasma Proteoforms with Engineered Nanoparticles for Top-down Proteomics
Deep Profiling of Plasma Proteoforms with Engineered Nanoparticles for Top-down Proteomics Open
The dynamic range challenge for detection of proteins and their proteoforms in human plasma has been well documented. Here, we use the nanoparticle protein corona approach to enrich low-abundant proteins selectively and reproducibly from h…
View article: Development of Ln(III) Derivatives as <sup>19</sup>F Parashift Probes
Development of Ln(III) Derivatives as <sup>19</sup>F Parashift Probes Open
19F parashift probes with paramagnetically shifted reporter nuclei provide attractive platforms to develop molecular imaging probes. These probes enable ratiometric detection of molecular disease markers using a direct detection technique.…
View article: Single Cell Analysis of Proteoforms
Single Cell Analysis of Proteoforms Open
We introduce single cell Proteoform imaging Mass Spectrometry (scPiMS), which realizes the benefit of direct solvent extraction and MS detection of intact proteins from single cells dropcast onto glass slides. Sampling and detection of who…
View article: Advances and prospects for the Human BioMolecular Atlas Program (HuBMAP)
Advances and prospects for the Human BioMolecular Atlas Program (HuBMAP) Open
The Human BioMolecular Atlas Program (HuBMAP) aims to create a multi-scale spatial atlas of the healthy human body at single-cell resolution by applying advanced technologies and disseminating resources to the community. As the HuBMAP move…
View article: Top-down Proteomics of 10,000 Single Brain Cells
Top-down Proteomics of 10,000 Single Brain Cells Open
We introduce single-cell Proteoform imaging Mass Spectrometry ( scPiMS ), which realizes the benefit of direct analysis of intact proteins to process 10,836 single cells from the rat hippocampus. This new platform addresses the throughput …
View article: NMR Relaxometry data for publication "Engineered Nonviral Protein Cages Modified for MR Imaging"
NMR Relaxometry data for publication "Engineered Nonviral Protein Cages Modified for MR Imaging" Open
NMRD profiles collected with field-cycling NMR relaxometry of a water solution of Gd-C4-IA, and of AaLS-13 and OP protein cages labeled with the gadolinium(III) complex. Published in: https://doi.org/10.1021/acsabm.2c00892 NMRD data acquis…
View article: Engineered Nonviral Protein Cages Modified for MR Imaging
Engineered Nonviral Protein Cages Modified for MR Imaging Open
Diagnostic medical imaging utilizes magnetic resonance (MR) to provide anatomical, functional, and molecular information in a single scan. Nanoparticles are often labeled with Gd(III) complexes to amplify the MR signal of contrast agents (…
View article: NMR Relaxometry data for publication "Engineered Nonviral Protein Cages Modified for MR Imaging"
NMR Relaxometry data for publication "Engineered Nonviral Protein Cages Modified for MR Imaging" Open
NMRD profiles collected with field-cycling NMR relaxometry of a water solution of Gd-C4-IA, and of AaLS-13 and OP protein cages labeled with the gadolinium(III) complex. Published in: https://doi.org/10.1021/acsabm.2c00892 NMRD data acquis…
View article: Mapping the KRAS proteoform landscape in colorectal cancer identifies truncated KRAS4B that decreases MAPK signaling
Mapping the KRAS proteoform landscape in colorectal cancer identifies truncated KRAS4B that decreases MAPK signaling Open
The KRAS gene is one of the most frequently mutated oncogenes in human cancer and gives rise to two isoforms, KRAS4A and KRAS4B. KRAS post-translational modifications (PTMs) have the potential to influence downstream signaling. However, th…
View article: BIOM-24. TARGETED NEXT-GENERATION SEQUENCING (NGS) OF TEMPORALLY MATCHED CEREBROSPINAL FLUID (CSF) AND TUMOR TISSUE IN PATIENTS WITH RECURRENT GLIOBLASTOMA (GBM)
BIOM-24. TARGETED NEXT-GENERATION SEQUENCING (NGS) OF TEMPORALLY MATCHED CEREBROSPINAL FLUID (CSF) AND TUMOR TISSUE IN PATIENTS WITH RECURRENT GLIOBLASTOMA (GBM) Open
BACKGROUND GBM genomic profiling relies on sequencing a limited tumor tissue sample, which is invasive and may underrepresent molecular heterogeneity. CSF, which can be obtained less invasively, is in intimate contact with tumor lesions an…
View article: A Ln(<scp>iii</scp>)-3-hydroxypyridine pH responsive probe optimized by DFT
A Ln(<span>iii</span>)-3-hydroxypyridine pH responsive probe optimized by DFT Open
The favorable pH response and coordination properties of the 3-hydroxypyridyl group indicate its potential for further development as a dual responsive-reporter group for Ln(iii) MRS probes.
View article: Self‐Immolative Activation of β‐Galactosidase‐Responsive Probes for In Vivo MR Imaging in Mouse Models
Self‐Immolative Activation of β‐Galactosidase‐Responsive Probes for In Vivo MR Imaging in Mouse Models Open
Our lab has developed a new series of self‐immolative MR agents for the rapid detection of enzyme activity in mouse models expressing β‐galactosidase (β‐gal). We investigated two molecular architectures to create agents that detect β‐gal a…
View article: Water-Soluble Nanoconjugate for Enhanced Cellular Delivery of Receptor-Targeted Magnetic Resonance Contrast Agents
Water-Soluble Nanoconjugate for Enhanced Cellular Delivery of Receptor-Targeted Magnetic Resonance Contrast Agents Open
ProGlo is an efficient steroid receptor-targeted magnetic resonance (MR) imaging contrast agent (CA). It has been shown to bind to the progesterone receptor (PR) and produce enhanced image contrast in PR-positive cells and tissues in vitro…