Michael A. R. Hollas
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View article: Proteoform profiling of endogenous single cells from rat hippocampus at scale
Proteoform profiling of endogenous single cells from rat hippocampus at scale Open
View article: Top-Down Proteomics Identifies Plasma Proteoform Signatures of Liver Cirrhosis Progression
Top-Down Proteomics Identifies Plasma Proteoform Signatures of Liver Cirrhosis Progression Open
Cirrhosis, advanced liver disease, affects 2 to 5 million Americans. While most patients have compensated cirrhosis and may be fairly asymptomatic, many decompensate and experience life-threatening complications such as gastrointestinal bl…
View article: Deep Profiling of Plasma Proteoforms with Engineered Nanoparticles for Top-Down Proteomics
Deep Profiling of Plasma Proteoforms with Engineered Nanoparticles for Top-Down Proteomics Open
The dynamic range challenge for the detection of proteins and their proteoforms in human plasma has been well documented. Here, we use the nanoparticle protein corona approach to enrich low-abundance proteins selectively and reproducibly f…
View article: Characterization of the Antibody Response to SARS-CoV-2 Infection in COVID-19 Transplant versus Nontransplant Recipients by Ig-MS
Characterization of the Antibody Response to SARS-CoV-2 Infection in COVID-19 Transplant versus Nontransplant Recipients by Ig-MS Open
Solid organ transplant recipients with immunosuppressant regimens to prevent rejection are less able to mount effective immune responses to pathogenic infection. Here, we apply a recently reported mass spectrometry-based serological approa…
View article: Deep Profiling of Plasma Proteoforms with Engineered Nanoparticles for Top-down Proteomics
Deep Profiling of Plasma Proteoforms with Engineered Nanoparticles for Top-down Proteomics Open
The dynamic range challenge for detection of proteins and their proteoforms in human plasma has been well documented. Here, we use the nanoparticle protein corona approach to enrich low-abundant proteins selectively and reproducibly from h…
View article: Top-Down Proteomics Identifies Plasma Proteoform Signatures of Liver Cirrhosis Progression
Top-Down Proteomics Identifies Plasma Proteoform Signatures of Liver Cirrhosis Progression Open
Cirrhosis, advanced liver disease, affects 2-5 million Americans. While most patients have compensated cirrhosis and may be fairly asymptomatic, many decompensate and experience life-threatening complications such as gastrointestinal bleed…
View article: Single Cell Analysis of Proteoforms
Single Cell Analysis of Proteoforms Open
We introduce single cell Proteoform imaging Mass Spectrometry (scPiMS), which realizes the benefit of direct solvent extraction and MS detection of intact proteins from single cells dropcast onto glass slides. Sampling and detection…
View article: Precise Readout of MEK1 Proteoforms upon MAPK Pathway Modulation by Individual Ion Mass Spectrometry
Precise Readout of MEK1 Proteoforms upon MAPK Pathway Modulation by Individual Ion Mass Spectrometry Open
The functions of proteins bearing multiple post-translational modifications (PTMs) are modulated by their modification patterns, yet precise characterization of them is difficult. MEK1 (also known as MAP2K1) is one such example that acts a…
View article: Author Correction: Automated imaging and identification of proteoforms directly from ovarian cancer tissue
Author Correction: Automated imaging and identification of proteoforms directly from ovarian cancer tissue Open
Tumor Stroma automated MS² 240 pixels MIF AT5G2
View article: Automated imaging and identification of proteoforms directly from ovarian cancer tissue
Automated imaging and identification of proteoforms directly from ovarian cancer tissue Open
View article: Identification of Splice Variants and Isoforms in Transcriptomics and Proteomics
Identification of Splice Variants and Isoforms in Transcriptomics and Proteomics Open
Alternative splicing is pivotal to the regulation of gene expression and protein diversity in eukaryotic cells. The detection of alternative splicing events requires specific omics technologies. Although short-read RNA sequencing has succe…
View article: Top-down Proteomics of 10,000 Single Brain Cells
Top-down Proteomics of 10,000 Single Brain Cells Open
We introduce single-cell Proteoform imaging Mass Spectrometry ( scPiMS ), which realizes the benefit of direct analysis of intact proteins to process 10,836 single cells from the rat hippocampus. This new platform addresses the throughput …
View article: Divergent Antibody Repertoires Found for Omicron versus Wuhan SARS-CoV-2 Strains Using Ig-MS
Divergent Antibody Repertoires Found for Omicron versus Wuhan SARS-CoV-2 Strains Using Ig-MS Open
SARS-CoV-2 Omicron (B.1.1.529) and its subvariants are currently the most common variants of concern worldwide, featuring numerous mutations in the spike protein and elsewhere that collectively make Omicron variants more transmissible and …
View article: Highly multiplexed, label-free proteoform imaging of tissues by individual ion mass spectrometry
Highly multiplexed, label-free proteoform imaging of tissues by individual ion mass spectrometry Open
Imaging of proteoforms in human tissues is hindered by low molecular specificity and limited proteome coverage. Here, we introduce proteoform imaging mass spectrometry (PiMS), which increases the size limit for proteoform detection and ide…
View article: Direct Imaging and Identification of Proteoforms up to 70 kDa from Human Tissue
Direct Imaging and Identification of Proteoforms up to 70 kDa from Human Tissue Open
Imaging of proteoforms in human tissues is hindered by low molecular specificity and limited proteome coverage. Here, we introduce proteoform imaging mass spectrometry (PiMS), which increases the size limit for proteoform detection and ide…
View article: The Human Proteoform Atlas: a FAIR community resource for experimentally derived proteoforms
The Human Proteoform Atlas: a FAIR community resource for experimentally derived proteoforms Open
The Human Proteoform Atlas (HPfA) is a web-based repository of experimentally verified human proteoforms on-line at http://human-proteoform-atlas.org and is a direct descendant of the Consortium of Top-Down Proteomics’ (CTDP) Proteoform At…
View article: Next-generation Serology by Mass Spectrometry: Readout of the SARS-CoV-2 Antibody Repertoire
Next-generation Serology by Mass Spectrometry: Readout of the SARS-CoV-2 Antibody Repertoire Open
Methods of antibody detection are used to assess exposure or immunity to a pathogen. Here, we present Ig-MS , a novel serological readout that captures the immunoglobulin (Ig) repertoire at molecular resolution, including entire variable r…
View article: Spin Labeling of Surface Cysteines Using a Bromoacrylaldehyde Spin Label
Spin Labeling of Surface Cysteines Using a Bromoacrylaldehyde Spin Label Open
Structural investigations of proteins and their biological complexes are now frequently complemented by distance constraints between spin labeled cysteines generated using double electron–electron resonance (DEER) spectroscopy, via site di…
View article: Interaction of oxidative stress and neurotrauma in ALDH2 mice causes significant and persistent behavioral and pro-inflammatory effects in a tractable model of mild traumatic brain injury
Interaction of oxidative stress and neurotrauma in ALDH2 mice causes significant and persistent behavioral and pro-inflammatory effects in a tractable model of mild traumatic brain injury Open
View article: Design and Synthesis of Basic Selective Estrogen Receptor Degraders for Endocrine Therapy Resistant Breast Cancer
Design and Synthesis of Basic Selective Estrogen Receptor Degraders for Endocrine Therapy Resistant Breast Cancer Open
The clinical steroidal selective estrogen receptor (ER) degrader (SERD), fulvestrant, is effective in metastatic breast cancer, but limited by poor pharmacokinetics, prompting the development of orally bioavailable, nonsteroidal SERDs, cur…
View article: Pharmacological manipulation of cGMP and NO/cGMP in CNS drug discovery
Pharmacological manipulation of cGMP and NO/cGMP in CNS drug discovery Open
View article: Adenylation Activity of Carboxylic Acid Reductases Enables the Synthesis of Amides
Adenylation Activity of Carboxylic Acid Reductases Enables the Synthesis of Amides Open
Carboxylic acid reductases (CARs) catalyze the reduction of a broad range of carboxylic acids to aldehydes using the cofactors adenosine triphosphate and nicotinamide adenine dinucleotide phosphate, and have become attractive biocatalysts …
View article: A Bifunctional Spin Label for Ligand Recognition on Surfaces
A Bifunctional Spin Label for Ligand Recognition on Surfaces Open
In situ monitoring of biomolecular recognition, especially at surfaces, still presents a significant technical challenge. Electron paramagnetic resonance (EPR) of biomolecules spin‐labeled with nitroxides can offer uniquely sensitive and s…
View article: A Bifunctional Spin Label for Ligand Recognition on Surfaces
A Bifunctional Spin Label for Ligand Recognition on Surfaces Open
In situ monitoring of biomolecular recognition, especially at surfaces, still presents a significant technical challenge. Electron paramagnetic resonance (EPR) of biomolecules spin‐labeled with nitroxides can offer uniquely sensitive and s…
View article: CCDC 1431285: Experimental Crystal Structure Determination
CCDC 1431285: Experimental Crystal Structure Determination Open
An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available …