Michael D. Bick
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View article: Increased binding of actinomycin D to halodeoxyuridine-substituted DNAs.
Increased binding of actinomycin D to halodeoxyuridine-substituted DNAs. Open
Medicine, New York, New York 10035 We have used equilibrium dialysis and other tech- niques to examine the actinomycin D binding sites on unsubstituted and halodeoxyuridine-substituted DNAs. Some of the actinomycin D binding sites present …
View article: Interaction of chromosomal proteins with BrdU substituted DNA as determined by chromatin-DNA competition
Interaction of chromosomal proteins with BrdU substituted DNA as determined by chromatin-DNA competition Open
Chromatin-DNA competition has been utilized to examine the general nature of chromosomal proteins interacting more strongly with BrdU substituted DNA. When chromatin is incubated with an excess of purified DNA, a portion of the chromosomal…
View article: Thermal denaturation of DNA from bromodeoxyuridine substituted cells
Thermal denaturation of DNA from bromodeoxyuridine substituted cells Open
The thermal denaturation of DNA from cell lines extensively substituted with bromodeoxyuridine has been examined spectrophotometrically over a wide range in ionic strength and by thermal elution from hydroxyapatite columns. BrdU substituti…
View article: Misincorporation of GTP during transcription of poly dAT-dAT and poly dABU-dABU
Misincorporation of GTP during transcription of poly dAT-dAT and poly dABU-dABU Open
The synthetic DNA alternating copolymers poly dAT-dAT and dABU-dABU have been transcribed with E. coli RNA polymerase to measure the level of BrdU-induced misincorporation of guanine during transcription. GTP is found to be misincorporated…
View article: A new method for mapping nucleic acid sequence homology by electron microscopy
A new method for mapping nucleic acid sequence homology by electron microscopy Open
We describe here a new method for the electron microscopic mapping of sequence homology in nucleic acids. Specific segments of the T7 chromosome have been isolated following digestion with the restriction endonuclease from Hemophilus aegyp…
View article: Total Substitution of Bromodeoxyuridine for Thymidine in the DNA of a Bromodeoxyuridine-Dependent Cell Line
Total Substitution of Bromodeoxyuridine for Thymidine in the DNA of a Bromodeoxyuridine-Dependent Cell Line Open
In an attempt to isolate cells that could survive with total replacement of thymidine by bromodeoxyuridine in nuclear DNA, cells of a bromodeoxyuridine-dependent Syrian hamster line were cultured in medium containing aminopterin and bromod…
View article: Molecular Nature of Mammalian Cell DNA in Alkaline Sucrose Gradients
Molecular Nature of Mammalian Cell DNA in Alkaline Sucrose Gradients Open
Mammalian cell DNA that exhibited anomalous sedimentation in alkaline sucrose gradients was examined directly by electron microscopy. Its appearance was that of duplex DNA. In addition, some duplex DNA was observed under conditions in whic…
View article: Bromodeoxyuridine Dependence—A New Mutation in Mammalian Cells
Bromodeoxyuridine Dependence—A New Mutation in Mammalian Cells Open
When cells of a Syrian hamster melanoma were grown in increasing concentrations of bromodeoxyuridine (BrdU), lines of mutant cells able to grow well at high concentrations of BrdU were isolated. The mutant cells were characterized by BrdU …
View article: Leucyl Transfer RNA Synthetase Changes during Soybean Cotyledon Senescence
Leucyl Transfer RNA Synthetase Changes during Soybean Cotyledon Senescence Open
Previous work has shown the complement of leucyl transfer RNA (tRNA Leu ) isoaccepting species present in soybean cotyledons to change during cotyledon senescence. Of the six tRNA Leu species resolved by Freon-column chromatography, the ca…