Michael Bouvet
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View article: The Combination of Recombinant Methioninase and Low-dose Chloroquine Selectively Eradicates Colon-Cancer Cells Without Apparent Toxicity on Co-cultured Normal Fibroblasts
The Combination of Recombinant Methioninase and Low-dose Chloroquine Selectively Eradicates Colon-Cancer Cells Without Apparent Toxicity on Co-cultured Normal Fibroblasts Open
rMETase has selective efficacy against cancer cells in the presence of normal cells, and its efficacy is significantly enhanced selectively on the cancer cells by CQ. The results of the present study suggest the potential for future clinic…
View article: Simultaneous Targeting of Multiple Hallmarks of Cancer With Recombinant Methioninase, Rapamycin and Chloroquine Is Specific and Synergistic to MiaPaCa-2 Pancreatic-Cancer Cells in Contrast to Hs-27 Normal Fibroblasts
Simultaneous Targeting of Multiple Hallmarks of Cancer With Recombinant Methioninase, Rapamycin and Chloroquine Is Specific and Synergistic to MiaPaCa-2 Pancreatic-Cancer Cells in Contrast to Hs-27 Normal Fibroblasts Open
The combination of rMETase, rapamycin and chloroquine had highly synergistic and selective efficacy against PDAC cells in vitro compared to normal cells, supporting its potential as a precision-targeted metabolic clinical therapy for pancr…
View article: Repurposing Carfilzomib as a Promising Drug for Targeted Therapy in Gastric Cancer
Repurposing Carfilzomib as a Promising Drug for Targeted Therapy in Gastric Cancer Open
Background/Objectives: Identifying novel targets to treat gastric cancer (GC) has become a focus of research in recent years. Our accelerated Helicobacter-induced gastric cancer mouse model allowed us to identify several differentially exp…
View article: CANDiT: A machine learning framework for differentiation therapy in colorectal cancer
CANDiT: A machine learning framework for differentiation therapy in colorectal cancer Open
Reactivating lineage commitment to differentiate, and hence eliminate, cancer stem cells (CSCs) remains a therapeutic challenge. Here, we present CANDiT (cancer-associated nodes for differentiation targeting), a machine learning framework …
View article: Recombinant Methioninase Selectively Eliminates Cancer Cells Co-cultured With Normal Fibroblasts Indicating the High-Precision Efficacy of Targeting Methionine Addiction of Cancer
Recombinant Methioninase Selectively Eliminates Cancer Cells Co-cultured With Normal Fibroblasts Indicating the High-Precision Efficacy of Targeting Methionine Addiction of Cancer Open
The differential sensitivity of cancer and normal cells to rMETase at 0.46 U/ml was so extensive that cancer cells were essentially eliminated from the co-cultures while the normal cells remained viable and proliferating. The difference in…
View article: Combinations of <i>Salmonella typhimurium</i> A1-R, Recombinant Methioninase, and Chloroquine, Each Targeting Fundamental Cancer Hallmarks, Are Selectively Effective on Colon Cancer Cells Compared to Normal Fibroblasts
Combinations of <i>Salmonella typhimurium</i> A1-R, Recombinant Methioninase, and Chloroquine, Each Targeting Fundamental Cancer Hallmarks, Are Selectively Effective on Colon Cancer Cells Compared to Normal Fibroblasts Open
Tumor-targeting with A1-R combined with methionine restriction (rMETase) or autophagy inhibition (CQ) resulted in selective and synergistic cytotoxicity against colon-cancer cells compared to normal fibroblasts. The present findings suppor…
View article: Brightly Visualizing Pancreatic Cancer Margins in Orthotopic Mouse Models with an Anti-CA19-9 Antibody Conjugated to a Near-Infrared Fluorophore
Brightly Visualizing Pancreatic Cancer Margins in Orthotopic Mouse Models with an Anti-CA19-9 Antibody Conjugated to a Near-Infrared Fluorophore Open
Background/Purpose: The only potentially curative procedure for pancreatic cancer is R0 resection, which is difficult to achieve due to poorly defined tumor margins. In the present study, we used an anti-CA19-9 antibody conjugated to a nea…
View article: The Triple Combination of Recombinant Methioninase, Rapamycin, and Chloroquine Synergistically Eradicates HCT116 Colon Cancer Cells
The Triple Combination of Recombinant Methioninase, Rapamycin, and Chloroquine Synergistically Eradicates HCT116 Colon Cancer Cells Open
The triple combination of rMETase, RAPA and CQ shows strong synergistic efficacy on the human HCT116 colon cancer cell line. The results of the present study suggest the potential for future clinical applications of this triple-combination…
View article: The Conditions of Non-rescuability of Methioninase-treated Cancer Cells by Methionine Replenishment: The Point of No Return
The Conditions of Non-rescuability of Methioninase-treated Cancer Cells by Methionine Replenishment: The Point of No Return Open
Three distinct responses to methionine replenishment following rMETase treatment were observed in HCT-116 cells. First, complete recovery and resumption of proliferation occurred with low-dose rMETase at all time points and with short-term…
View article: Mucin4 (MUC4) Antibody Labeled with an NIR Dye Brightly Targets Pancreatic Cancer Liver Metastases and Peritoneal Carcinomatosis
Mucin4 (MUC4) Antibody Labeled with an NIR Dye Brightly Targets Pancreatic Cancer Liver Metastases and Peritoneal Carcinomatosis Open
Background/Objectives: Pancreatic cancer is the fourth leading cause of deaths related to cancer. It is a highly aggressive malignancy and often metastasizes quickly to other parts of the body and organs. The most effective cure is surgica…
View article: Human fibrosarcoma cells selected for very-high doxorubicin resistance, acquire trabectedin and eribulin cross-resistance, remain sensitive to recombinant methioninase, and have increased c-MYC expression
Human fibrosarcoma cells selected for very-high doxorubicin resistance, acquire trabectedin and eribulin cross-resistance, remain sensitive to recombinant methioninase, and have increased c-MYC expression Open
Doxorubicin is first-line chemotherapy for soft tissue sarcoma; however, the development of drug resistance limits its efficacy. The purpose of the present study was to select very-high doxorubicin-resistant (VHDR) HT1080 fibrosarcoma cell…
View article: Tumor Immunology Visualized in Realtime in<i>In-vivo</i>-like 3-D Gelfoam<sup>®</sup>Histoculture by Color-coded Imaging
Tumor Immunology Visualized in Realtime in<i>In-vivo</i>-like 3-D Gelfoam<sup>®</sup>Histoculture by Color-coded Imaging Open
The present results demonstrate a new 3-D co-culture color-coded system to visualize in real-time the interaction of PBMCs and cancer cells using fluorescence color-coded imaging. Future experiments will test drugs that can stimulate immun…
View article: Liposomal oncolytic adenovirus as a neoadjuvant therapy for triple-negative breast cancer
Liposomal oncolytic adenovirus as a neoadjuvant therapy for triple-negative breast cancer Open
Breast cancer remains one of the leading causes of cancer-related death, with triple-negative breast cancer (TNBC) accounting for 15–20% of cases. TNBC, characterized by the absence of ER, PR, and HER2 protein, is an aggressive form of bre…
View article: Potential higher malignancy of nonfunctional parathyroid carcinoma: a case series study
Potential higher malignancy of nonfunctional parathyroid carcinoma: a case series study Open
The poor prognosis of patients with nonfunctional parathyroid carcinoma may reflect biological behavior and a potentially higher malignant potential. The Ki-67 level is positively correlated with the malignant potential of parathyroid carc…
View article: HT1080 Human Fibrosarcoma Cells Selected for Super-eribulin Resistance <i>In Vitro</i> Become More Malignant and Are Arrested Synergistically by Methionine Restriction in Combination With Eribulin in Nude Mice
HT1080 Human Fibrosarcoma Cells Selected for Super-eribulin Resistance <i>In Vitro</i> Become More Malignant and Are Arrested Synergistically by Methionine Restriction in Combination With Eribulin in Nude Mice Open
Methionine restriction combined with eribulin represents a promising strategy to effectively treat eribulin-resistant high-malignancy fibrosarcoma, that can be immediately applied to the clinic.
View article: Combination of Recombinant Methioninase With Rapamycin or Chloroquine Is Synergistic to Highly Inhibit Triple-negative Breast Cancer Cells<i>In Vitro</i>
Combination of Recombinant Methioninase With Rapamycin or Chloroquine Is Synergistic to Highly Inhibit Triple-negative Breast Cancer Cells<i>In Vitro</i> Open
rMETase, when combined with either RAPA or CQ, is synergistic on the MDA-MB-231 TNBC cell line. The present findings suggest the potential for future clinical applications of rMETase plus chemotherapy, such as RAPA or CQ, for recalcitrant …
View article: Value of Neuroradiology Second Reads of CT Scans for Hyperparathyroidism
Value of Neuroradiology Second Reads of CT Scans for Hyperparathyroidism Open
Background/Objectives: Parathyroidectomy is a curative procedure for primary hyperparathyroidism, and multi-phase CT is an integral part of surgical planning. While patients may be referred to centers specializing in endocrine surgery, the…
View article: Highly Synergistic Eradication of 143B Osteosarcoma Cells<i>In Vitro</i>by the Combination of Recombinant Methioninase, Chloroquine, and Rapamycin Targeting Methionine Addiction, Autophagy, and mTOR, Respectively
Highly Synergistic Eradication of 143B Osteosarcoma Cells<i>In Vitro</i>by the Combination of Recombinant Methioninase, Chloroquine, and Rapamycin Targeting Methionine Addiction, Autophagy, and mTOR, Respectively Open
The triple combination of rMETase, CQ, and RAPA demonstrated strong synergy and effectively eradicated 143B osteosarcoma cells. Therefore, the triple treatment with rMETase, CQ, and RAPA has potential as a novel, effective therapeutic appr…
View article: Super Methotrexate-resistant Osteosarcoma Cells Retain Their Sensitivity to Recombinant Methioninase: Targeting Methionine Addiction to Overcome Extreme Cancer-Chemotherapy Resistance
Super Methotrexate-resistant Osteosarcoma Cells Retain Their Sensitivity to Recombinant Methioninase: Targeting Methionine Addiction to Overcome Extreme Cancer-Chemotherapy Resistance Open
rMETase overcame a 5,500-fold MTX-resistance of osteosarcoma cells. The present results suggest methionine restriction by rMETase can be a potential clinical strategy to overcome recalcitrant drug-resistance in osteosarcoma.
View article: HT1080 Fibrosarcoma With Acquired Trabectedin Resistance: Increased Malignancy But Sustained Sensitivity to Methionine Restriction
HT1080 Fibrosarcoma With Acquired Trabectedin Resistance: Increased Malignancy But Sustained Sensitivity to Methionine Restriction Open
TR-HT1080 cells acquired high malignancy by in vitro selection for trabectedin resistance. However, methionine restriction overcame trabectedin resistance in vivo, strongly inhibiting tumor growth, which should be further investigated in t…
View article: Dynamic Fluorescence Imaging of Orally-administered Tumor-targeting<i>Salmonella typhimurium</i>A1-R Expressing Green Fluorescent Protein Trafficking Through the Gastrointestinal System to Target Fibrosarcomas in Nude Mice
Dynamic Fluorescence Imaging of Orally-administered Tumor-targeting<i>Salmonella typhimurium</i>A1-R Expressing Green Fluorescent Protein Trafficking Through the Gastrointestinal System to Target Fibrosarcomas in Nude Mice Open
The present study showed the pathway of orally administered A1-R-GFP in the gastrointestinal system and to the tumor and liver. A1-R selectively proliferated continuously in tumors and was cleared from the liver. These results are critical…
View article: Engineered Methioninase-expressing Tumor-targeting<i>Salmonella typhimurium</i>A1-R Inhibits Syngeneic-Cancer Mouse Models by Depleting Tumor Methionine
Engineered Methioninase-expressing Tumor-targeting<i>Salmonella typhimurium</i>A1-R Inhibits Syngeneic-Cancer Mouse Models by Depleting Tumor Methionine Open
Tumor-targeting Salmonella typhimurium A1-R engineered to express the Pseudomonas putida methioninase gene, inhibited LLC tumor growth in a syngeneic mouse model and reduced the methionine level in the tumor. Salmonella typhimurium A1-R-rM…
View article: Targeting Methionine Addiction of Osteosarcoma with Methionine Restriction to Overcome Drug Resistance: A New Paradigm for a Recalcitrant Disease
Targeting Methionine Addiction of Osteosarcoma with Methionine Restriction to Overcome Drug Resistance: A New Paradigm for a Recalcitrant Disease Open
Chemotherapy resistance in osteosarcoma results in a very poor patient prognosis, with the 5-year survival rate of approximately 20%, which not improved for over three decades; thus, the development of novel therapeutic strategies is requi…
View article: Recombinant Methioninase (rMETase) Synergistically Sensitizes Ivermectin-resistant MCF-7 Breast Cancer Cells 9.9 Fold to Low-dose Ivermectin
Recombinant Methioninase (rMETase) Synergistically Sensitizes Ivermectin-resistant MCF-7 Breast Cancer Cells 9.9 Fold to Low-dose Ivermectin Open
The combination of ivermectin and rMETase had synergistic efficacy against MCF-7 breast cancer cells in vitro. The present findings suggest that the combination of ivermectin and rMETase is a promising strategy for breast cancer requiring …
View article: Elevated-c-MYC-expressing Fibrosarcoma Cells With Acquired Gemcitabine Resistance Remain Sensitive to Recombinant Methioninase: A Potential Clinical Strategy for a Recalcitrant Disease
Elevated-c-MYC-expressing Fibrosarcoma Cells With Acquired Gemcitabine Resistance Remain Sensitive to Recombinant Methioninase: A Potential Clinical Strategy for a Recalcitrant Disease Open
Background/Aim: For second-line chemotherapy of soft-tissue sarcoma, gemcitabine is administered in combination with docetaxel. However, more effective treatments are required for advanced soft-tissue sarcoma, where the efficacy is limited…
View article: The Combination of Tumor-targeting<i>Salmonella typhimurium</i>A1-R Plus the Autophagy-inhibitor Chloroquine Synergistically Eradicates HT1080 Fibrosarcoma Cells<i>In Vitro</i>and<i>In Vivo</i>
The Combination of Tumor-targeting<i>Salmonella typhimurium</i>A1-R Plus the Autophagy-inhibitor Chloroquine Synergistically Eradicates HT1080 Fibrosarcoma Cells<i>In Vitro</i>and<i>In Vivo</i> Open
The combination treatment of A1-R plus chloroquine demonstrated synergy against HT1080 cancer cells in vitro and in vivo. A1-R was administered orally, suggesting its potential as a probiotic. The present results suggest the clinical poten…
View article: Synergistic Eradication of Fibrosarcoma With Acquired Ifosfamide Resistance Using Methionine Restriction Combined With Ifosfamide in Nude-mouse Models
Synergistic Eradication of Fibrosarcoma With Acquired Ifosfamide Resistance Using Methionine Restriction Combined With Ifosfamide in Nude-mouse Models Open
The present results suggest the combination of MR and ifosfamide has promising potential for overcoming ifosfamide resistance in future clinical applications.