Michael Brad Strader
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View article: Albuminuria is associated with worse outcomes in non‐diabetics hospitalized with acute heart failure
Albuminuria is associated with worse outcomes in non‐diabetics hospitalized with acute heart failure Open
Aims Chronic kidney disease (CKD) increases the risk of morbidity and mortality in patients with heart failure (HF). Guidelines recommend assessing CKD using the urinary albumin‐to‐creatinine ratio (UACR), as albuminuria is strongly associ…
View article: Identifying immunodominant proteins in mouse urine
Identifying immunodominant proteins in mouse urine Open
View article: #3385 The short and long-term accuracy of urinary C-C motif Chemokine-14 in an ICU population
#3385 The short and long-term accuracy of urinary C-C motif Chemokine-14 in an ICU population Open
Background and Aims Acute Kidney Injury (AKI) is a life-threatening syndrome associated with significant short- and long-term negative outcomes. Current AKI clinical biomarkers, such as serum creatinine and urine output, are limited. The D…
View article: #535 Audit of the clinical implementation of urinary NGAL in the diagnostic work-up of acute kidney injury at an Irish Hospital
#535 Audit of the clinical implementation of urinary NGAL in the diagnostic work-up of acute kidney injury at an Irish Hospital Open
Background and Aims Differentiating functional acute kidney injury (AKI) from structural tubular injury AKI remains challenging with existing clinical tools. urinary NGAL (uNGAL) has shown promise in distinguishing functional AKI, such as …
View article: Clinical Implementation of Urinary NGAL Testing for Diagnosing Acute Kidney Injury in an Academic Tertiary Care Medical Centre
Clinical Implementation of Urinary NGAL Testing for Diagnosing Acute Kidney Injury in an Academic Tertiary Care Medical Centre Open
Background: Differentiating functional acute kidney injury (AKI) from structural/intrinsic AKI with tubular injury remains a clinical challenge. Urinary NGAL (uNGAL) has shown promise in distinguishing these conditions. This study evaluate…
View article: Novel Biomarkers for Rejection in Kidney Transplantation: A Comprehensive Review
Novel Biomarkers for Rejection in Kidney Transplantation: A Comprehensive Review Open
Kidney transplantation is the treatment of choice for patients with end-stage kidney disease. Despite significant advances in graft survival, rejection continues to pose a major clinical challenge. Conventional monitoring tools, such as se…
View article: The Path to Precision in Acute Kidney Injury
The Path to Precision in Acute Kidney Injury Open
View article: Early and Sensitive Detection of Cisplatin-Induced Kidney Injury Using Novel Biomarkers
Early and Sensitive Detection of Cisplatin-Induced Kidney Injury Using Novel Biomarkers Open
NBMs accurately and timely detected cisplatin exposure and identified "sub-clinical" DIKI undetected by standard acute kidney injury (AKI) criteria, highlighting the limitations of current functional BMs in estimating the true DIKI inciden…
View article: Identifying Immunodominant Major Urinary Proteins (MUPs) In Mouse Urine
Identifying Immunodominant Major Urinary Proteins (MUPs) In Mouse Urine Open
View article: Proteomic evaluation of spore and hyphae proteomes of Alternaria alternata
Proteomic evaluation of spore and hyphae proteomes of Alternaria alternata Open
View article: Proteomic evaluation of Alternaria alternata spores, hyphae, and commercial allergen extracts
Proteomic evaluation of Alternaria alternata spores, hyphae, and commercial allergen extracts Open
View article: The Providence Mutation (βK82D) in Human Hemoglobin Substantially Reduces βCysteine 93 Oxidation and Oxidative Stress in Endothelial Cells
The Providence Mutation (βK82D) in Human Hemoglobin Substantially Reduces βCysteine 93 Oxidation and Oxidative Stress in Endothelial Cells Open
The highly toxic oxidative transformation of hemoglobin (Hb) to the ferryl state (HbFe4+) is known to occur in both in vitro and in vivo settings. We recently constructed oxidatively stable human Hbs, based on the Hb Providence (βK82D) mut…
View article: Post-translational modification as a response to cellular stress induced by hemoglobin oxidation in sickle cell disease
Post-translational modification as a response to cellular stress induced by hemoglobin oxidation in sickle cell disease Open
View article: Antisickling Drugs Targeting βCys93 Reduce Iron Oxidation and Oxidative Changes in Sickle Cell Hemoglobin
Antisickling Drugs Targeting βCys93 Reduce Iron Oxidation and Oxidative Changes in Sickle Cell Hemoglobin Open
Sickle cell disease is a genetic blood disorder caused by a single point mutation in the β globin gene where glutamic acid is replaced by valine at the sixth position of the β chain of hemoglobin (Hb). At low oxygen tension, the polymeriza…
View article: Interactions of an Anti-Sickling Drug with Hemoglobin in Red Blood Cells from a Patient with Sickle Cell Anemia
Interactions of an Anti-Sickling Drug with Hemoglobin in Red Blood Cells from a Patient with Sickle Cell Anemia Open
The pathophysiology associated with sickle cell disease (SCD) includes hemolytic anemia, vaso-occlusive events, and ultimately end organ damage set off by the polymerization of deoxygenated hemoglobin S (HbS) into long fibers and sickling …
View article: Substitutions in the β subunits of sickle-cell hemoglobin improve oxidative stability and increase the delay time of sickle-cell fiber formation
Substitutions in the β subunits of sickle-cell hemoglobin improve oxidative stability and increase the delay time of sickle-cell fiber formation Open
View article: Hemoglobin oxidation–dependent reactions promote interactions with band 3 and oxidative changes in sickle cell–derived microparticles
Hemoglobin oxidation–dependent reactions promote interactions with band 3 and oxidative changes in sickle cell–derived microparticles Open
The contribution of intracellular hemoglobin (Hb) oxidation to RBC-derived microparticle (MP) formation is poorly defined in sickle cell disease (SCD). Here we report that sickle Hb (HbS) oxidation, coupled with changes in cytosolic antiox…
View article: Site-directed mutagenesis of cysteine residues alters oxidative stability of fetal hemoglobin
Site-directed mutagenesis of cysteine residues alters oxidative stability of fetal hemoglobin Open
View article: Comparison of the oxidative reactivity of recombinant fetal and adult human hemoglobin: implications for the design of hemoglobin-based oxygen carriers
Comparison of the oxidative reactivity of recombinant fetal and adult human hemoglobin: implications for the design of hemoglobin-based oxygen carriers Open
Hemoglobin (Hb)-based oxygen carriers (HBOCs) have been engineered to replace or augment the oxygen carrying capacity of erythrocytes. However, clinical results have generally been disappointing, in part due to the intrinsic oxidative toxi…
View article: Dissecting the biochemical architecture and morphological release pathways of the human platelet extracellular vesiculome
Dissecting the biochemical architecture and morphological release pathways of the human platelet extracellular vesiculome Open
View article: Who accesses surgery at district level in sub‐Saharan Africa? Evidence from Malawi and Zambia
Who accesses surgery at district level in sub‐Saharan Africa? Evidence from Malawi and Zambia Open
Objectives To examine age and gender distribution for the most common types of surgery in Malawi and Zambia. Methods Data were collected from major operating theatres in eight district hospitals in Malawi and nine in Zambia. Raw data on su…
View article: Heterogeneity in non‐epitope loop sequence and outer membrane protein complexes alters antibody binding to the major porin protein PorB in serogroup B <i>Neisseria meningitidis</i>
Heterogeneity in non‐epitope loop sequence and outer membrane protein complexes alters antibody binding to the major porin protein PorB in serogroup B <i>Neisseria meningitidis</i> Open
Summary PorB is a well‐characterized outer membrane protein that is common among Neisseria species and is required for survival. A vaccine candidate, PorB induces antibody responses that are directed against six variable surface‐exposed lo…
View article: Evaluation of Stem Cell-Derived Red Blood Cells as a Transfusion Product Using a Novel Animal Model
Evaluation of Stem Cell-Derived Red Blood Cells as a Transfusion Product Using a Novel Animal Model Open
Reliance on volunteer blood donors can lead to transfusion product shortages, and current liquid storage of red blood cells (RBCs) is associated with biochemical changes over time, known as 'the storage lesion'. Thus, there is a need for a…
View article: Exploring Oxidative Reactions in Hemoglobin Variants Using Mass Spectrometry: Lessons for Engineering Oxidatively Stable Oxygen Therapeutics
Exploring Oxidative Reactions in Hemoglobin Variants Using Mass Spectrometry: Lessons for Engineering Oxidatively Stable Oxygen Therapeutics Open
Future HRAM studies are aimed at fully characterizing engineered candidate HBOCs to determine the most oxidatively stable protein while retaining oxygen carrying function in vivo. Antioxid. Redox Signal. 26, 777-793.
View article: Oxidative instability of hemoglobin E (β26 Glu→Lys) is increased in the presence of free α subunits and reversed by α-hemoglobin stabilizing protein (AHSP): Relevance to HbE/β-thalassemia
Oxidative instability of hemoglobin E (β26 Glu→Lys) is increased in the presence of free α subunits and reversed by α-hemoglobin stabilizing protein (AHSP): Relevance to HbE/β-thalassemia Open
When adding peroxide (H2O2), β subunits of hemoglobin (Hb) bear the burden of oxidative changes due in part to the direct oxidation of its Cys93. The presence of unpaired α subunits within red cells and/or co-inheritance of another β subun…
View article: Tales of Dihydrofolate Binding to R67 Dihydrofolate Reductase
Tales of Dihydrofolate Binding to R67 Dihydrofolate Reductase Open
Homotetrameric R67 dihydrofolate reductase possesses 222 symmetry and a single active site pore. This situation results in a promiscuous binding site that accommodates either the substrate, dihydrofolate (DHF), or the cofactor, NADPH. NADP…
View article: Sickle Cell Hemoglobin in the Ferryl State Promotes βCys-93 Oxidation and Mitochondrial Dysfunction in Epithelial Lung Cells (E10)
Sickle Cell Hemoglobin in the Ferryl State Promotes βCys-93 Oxidation and Mitochondrial Dysfunction in Epithelial Lung Cells (E10) Open
Polymerization of intraerythrocytic deoxyhemoglobin S (HbS) is the primary molecular event that leads to hemolytic anemia in sickle cell disease (SCD). We reasoned that HbS may contribute to the complex pathophysiology of SCD in part due t…
View article: Dissection of the radical reactions linked to fetal hemoglobin reveals enhanced pseudoperoxidase activity
Dissection of the radical reactions linked to fetal hemoglobin reveals enhanced pseudoperoxidase activity Open
In the presence of excess hydrogen peroxide (H2O2), ferrous (Fe(+2)) human hemoglobin (Hb) (α2β2) undergoes a rapid conversion to a higher oxidation ferryl state (Fe(+4)) which rapidly autoreduces back to the ferric form (Fe(+3)) as H2O2 i…