Peter M. Brophy
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View article: Body condition score and weight are effective targeted selective treatment indicators for gastrointestinal nematodes in premating ewes
Body condition score and weight are effective targeted selective treatment indicators for gastrointestinal nematodes in premating ewes Open
Background Ewe reproductive performance is key for flock profitability but can be impacted upon by gastrointestinal nematode (GIN) infections. Farmers commonly deworm ewes pre‐mating, yet concerns regarding anthelmintic resistance increase…
View article: Detoxome Capacity of the Adult Rumen Fluke <i>Calicophoron daubneyi</i> Extends into Its Secreted Extracellular Vesicles
Detoxome Capacity of the Adult Rumen Fluke <i>Calicophoron daubneyi</i> Extends into Its Secreted Extracellular Vesicles Open
Helminth parasites have long adapted to survive hostile host environments and can likely adapt against the chemical anthelmintic challenge. One proposed adaptation route is via Phase I and II xenobiotic metabolizing enzymes (XMEs). For suc…
View article: Assessing periparturient ewe characteristics and nemabiome composition to guide targeted selective treatment for sustainable gastrointestinal nematodes control in sheep
Assessing periparturient ewe characteristics and nemabiome composition to guide targeted selective treatment for sustainable gastrointestinal nematodes control in sheep Open
Gastrointestinal nematodes (GINs) are a significant threat to the sustainability of global sheep production. Periparturient ewes play a key role in GIN epidemiology, with increased GIN faecal egg counts (FECs) in these ewes resulting in he…
View article: A dominance of Mu class glutathione transferases within the equine tapeworm <i>Anoplocephala perfoliata</i>
A dominance of Mu class glutathione transferases within the equine tapeworm <i>Anoplocephala perfoliata</i> Open
The most common equine tapeworm, Anoplocephala perfoliata , has often been neglected amongst molecular investigations and has been faced with limited treatment options. However, the recent release of a transcriptome dataset has now provide…
View article: Discovery of long non-coding RNAs in the liver fluke, Fasciola hepatica
Discovery of long non-coding RNAs in the liver fluke, Fasciola hepatica Open
Long non-coding (lnc)RNAs are a class of eukaryotic RNA that do not code for protein and are linked with transcriptional regulation, amongst a myriad of other functions. Using a custom in silico pipeline we have identified 6,436 putative l…
View article: Discovery of long non-coding RNAs in the liver fluke,<i>Fasciola hepatica</i>
Discovery of long non-coding RNAs in the liver fluke,<i>Fasciola hepatica</i> Open
Long non-coding (lnc)RNAs are a class of eukaryotic RNA that do not code for protein and are linked with transcriptional regulation, amongst a myriad of other functions. Using a custom in silico pipeline we have identified 6,436 putative l…
View article: Identification of factors associated with <i>Fasciola hepatica</i> infection risk areas on pastures via an environmental <scp>DNA</scp> survey of <i>Galba truncatula</i> distribution using droplet digital and quantitative real‐time <scp>PCR</scp> assays
Identification of factors associated with <i>Fasciola hepatica</i> infection risk areas on pastures via an environmental <span>DNA</span> survey of <i>Galba truncatula</i> distribution using droplet digital and quantitative real‐time <span>PCR</span> assays Open
Environmental DNA (eDNA) is a powerful tool for identifying the spatial and temporal presence and density of species in a range of aquatic habitats. The analysis of eDNA has a wide range of application, one of which may be to inform of Fas…
View article: Associations between Gastrointestinal Nematode Infection Burden and Lying Behaviour as Measured by Accelerometers in Periparturient Ewes
Associations between Gastrointestinal Nematode Infection Burden and Lying Behaviour as Measured by Accelerometers in Periparturient Ewes Open
The application of precision livestock farming (PLF) technologies will underpin new strategies to support the control of livestock disease. However, PLF technology is underexploited within the sheep industry compared to other livestock sec…
View article: <i>Fasciola hepatica</i> Cathepsin L Zymogens: Immuno-Proteomic Evidence for Highly Immunogenic Zymogen-Specific Conformational Epitopes to Support Diagnostics Development
<i>Fasciola hepatica</i> Cathepsin L Zymogens: Immuno-Proteomic Evidence for Highly Immunogenic Zymogen-Specific Conformational Epitopes to Support Diagnostics Development Open
Fasciola hepatica, the common liver fluke and causative agent of zoonotic fasciolosis, impacts on food security with global economic losses of over $3.2 BN per annum through deterioration of animal health, productivity losses, and l…
View article: <i>In silico</i> characterisation of the complete Ly6 protein family in <i>Fasciola gigantica</i> supported through transcriptomics of the newly-excysted juveniles
<i>In silico</i> characterisation of the complete Ly6 protein family in <i>Fasciola gigantica</i> supported through transcriptomics of the newly-excysted juveniles Open
Incorporating the recently available F. gigantica genome, the current work revealed 20 novel Ly6 family members in F. gigantica and, in parallel, significantly extended the F. hepatica complement from 3 to 18 members.
View article: Evidence of Immune Modulators in the Secretome of the Equine Tapeworm Anoplocephala perfoliata
Evidence of Immune Modulators in the Secretome of the Equine Tapeworm Anoplocephala perfoliata Open
Anoplocephala perfoliata is a neglected gastro-intestinal tapeworm, commonly infecting horses worldwide. Molecular investigation of A. perfoliata is hampered by a lack of tools to better understand the host–parasite interface. This interfa…
View article: Temporal dynamics of trematode intermediate snail host environmental DNA in small water body habitats
Temporal dynamics of trematode intermediate snail host environmental DNA in small water body habitats Open
Environmental DNA (eDNA) surveying has potential to become a powerful tool for sustainable parasite control. As trematode parasites require an intermediate snail host that is often aquatic or amphibious to fulfil their lifecycle, water-bas…
View article: Evaluation of two Fasciola hepatica faecal egg counting protocols in sheep and cattle
Evaluation of two Fasciola hepatica faecal egg counting protocols in sheep and cattle Open
View article: Modulation of Rumen Microbes Through Extracellular Vesicle Released by the Rumen Fluke Calicophoron daubneyi
Modulation of Rumen Microbes Through Extracellular Vesicle Released by the Rumen Fluke Calicophoron daubneyi Open
Parasite derived extracellular vesicles (EVs) have been proposed to play key roles in the establishment and maintenance of infection. Calicophoron daubneyi is a newly emerging parasite of livestock with many aspects of its underpinning bio…
View article: 179 Reproductive performance of ewes following pre-mating targeted selective treatment against gastrointestinal nematodes
179 Reproductive performance of ewes following pre-mating targeted selective treatment against gastrointestinal nematodes Open
View article: Gastrointestinal nematode control practices in ewes: Identification of factors associated with application of control methods known to influence anthelmintic resistance development
Gastrointestinal nematode control practices in ewes: Identification of factors associated with application of control methods known to influence anthelmintic resistance development Open
View article: In vitro screening as an anthelmintic discovery pipeline for Calicophoron daubneyi: nutritive media and rumen environment-based approaches
In vitro screening as an anthelmintic discovery pipeline for Calicophoron daubneyi: nutritive media and rumen environment-based approaches Open
View article: The soluble glutathione transferase superfamily: role of Mu class in triclabendazole sulphoxide challenge in Fasciola hepatica
The soluble glutathione transferase superfamily: role of Mu class in triclabendazole sulphoxide challenge in Fasciola hepatica Open
Fasciola hepatica (liver fluke), a significant threat to food security, causes global economic loss for the livestock industry and is re-emerging as a foodborne disease of humans. In the absence of vaccines, treatment control is by anthelm…
View article: Rapid detection of Galba truncatula in water sources on pasture-land using loop-mediated isothermal amplification for control of trematode infections
Rapid detection of Galba truncatula in water sources on pasture-land using loop-mediated isothermal amplification for control of trematode infections Open
View article: RNA interference dynamics in juvenile Fasciola hepatica are altered during in vitro growth and development
RNA interference dynamics in juvenile Fasciola hepatica are altered during in vitro growth and development Open
View article: Evidence of sequestration of triclabendazole and associated metabolites by extracellular vesicles of Fasciola hepatica
Evidence of sequestration of triclabendazole and associated metabolites by extracellular vesicles of Fasciola hepatica Open
View article: Pilot Evaluation of Two Fasciola hepatica Biomarkers for Supporting Triclabendazole (TCBZ) Efficacy Diagnostics
Pilot Evaluation of Two Fasciola hepatica Biomarkers for Supporting Triclabendazole (TCBZ) Efficacy Diagnostics Open
Fasciola hepatica, the causative agent of fasciolosis, is a global threat to public health, animal welfare, agricultural productivity, and food security. In the ongoing absence of a commercial vaccine, independent emergences of anthelminti…
View article: Novel Equine Faecal Egg Diagnostics: Validation of the FECPAKG2
Novel Equine Faecal Egg Diagnostics: Validation of the FECPAKG2 Open
Faecal egg counts (FECs) are the standard method of diagnosing the level of parasitic helminth egg shedding in horses and other grazing animals. Testing before treatment is an important factor in slowing the appearance of anthelmintic resi…
View article: RNA interference dynamics in juvenile<i>Fasciola hepatica</i>are altered during<i>in vitro</i>growth and development
RNA interference dynamics in juvenile<i>Fasciola hepatica</i>are altered during<i>in vitro</i>growth and development Open
For over a decade RNA interference (RNAi) has been an important molecular tool for functional genomics studies in parasitic flatworms. Despite this, our understanding of RNAi dynamics in many flatworm parasites, such as the temperate liver…
View article: The importance of extracellular vesicle purification for downstream analysis: A comparison of differential centrifugation and size exclusion chromatography for helminth pathogens
The importance of extracellular vesicle purification for downstream analysis: A comparison of differential centrifugation and size exclusion chromatography for helminth pathogens Open
The data suggests that DC and SEC purification methods do not isolate equivalent EV population profiles and caution should be taken in the choice of EV purification utilised, with certain protocols for DC preparations including more free E…
View article: X-ray structure of Fasciola hepatica Sigma class glutathione transferase 1 reveals a disulfide bond to support stability in gastro-intestinal environment
X-ray structure of Fasciola hepatica Sigma class glutathione transferase 1 reveals a disulfide bond to support stability in gastro-intestinal environment Open
Sigma class GST (Prostaglandin D synthase), FhGST-S1, is present in the excretory–secretory products (ES) of the liver fluke parasite Fasciola hepatica as cargo of extracellular vesicles (EVs) released by the parasite. FhGST-S1 has a well …
View article: Smell of Infection: A Novel, Noninvasive Method for Detection of Fish Excretory-Secretory Proteins
Smell of Infection: A Novel, Noninvasive Method for Detection of Fish Excretory-Secretory Proteins Open
Chemical signals are produced by aquatic organisms following predatory attacks or perturbations such as parasitic infection. Ectoparasites feeding on fish hosts are likely to cause release of similar alarm cues into the environment due to …
View article: Polyomic tools for an emerging livestock parasite, the rumen fluke Calicophoron daubneyi; identifying shifts in rumen functionality
Polyomic tools for an emerging livestock parasite, the rumen fluke Calicophoron daubneyi; identifying shifts in rumen functionality Open
View article: Detection of Galba truncatula, Fasciola hepatica and Calicophoron daubneyi environmental DNA within water sources on pasture land, a future tool for fluke control?
Detection of Galba truncatula, Fasciola hepatica and Calicophoron daubneyi environmental DNA within water sources on pasture land, a future tool for fluke control? Open
View article: Purification of native Sigma class glutathione transferase from Fasciola hepatica
Purification of native Sigma class glutathione transferase from Fasciola hepatica Open