Michael C. Ayers
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CUT&Tag Identifies Repetitive Genomic Loci that are Excluded from ChIP Assays Open
Summary Determining the genomic localization of chromatin features is an essential aspect of investigating gene expression control, and ChIP-Seq has long been the gold standard technique for interrogating chromatin landscapes. Recently, th…
Laboratory evolutions lead to reproducible mutations in PDR3 conferring resistance to MCHM Open
The solubility of protein complexes and membraneless compartments is maintained by liquid-liquid phase separation (LLPS). Phase transition is induced or dissolved by biological hydrotropes such as ATP and RNA. 4-methylcyclohexane methanol …
4-Methylcyclohexane methanol (MCHM) affects viability, development, and movement of Xenopus embryos Open
Following chemical spill disasters, it is important to estimate the effects of spilled chemicals on humans and the environment. Here we analyzed the toxicological effects of the coal cleaning chemical, 4-methylcyclohexane methanol (MCHM), …
Oxidative Stress Responses and Nutrient Starvation in MCHM Treated <i>Saccharomyces cerevisiae</i> Open
In 2014, the coal cleaning chemical 4-methylcyclohexane methanol (MCHM) spilled into the water supply for 300,000 West Virginians. Initial toxicology tests showed relatively mild results, but the underlying effects on cellular biology were…
Oxidative Stress Responses and Nutrient Starvation in MCHM Treated<i>Saccharomyces cerevisiae</i> Open
In 2014, the coal cleaning chemical 4-methylcyclohexane methanol (MCHM) spilled into the water supply for 300,000 West Virginians. Initial toxicology tests showed relatively mild results, but the underlying effects on cellular biology were…
The Polymorphic PolyQ Tail Protein of the Mediator Complex, Med15, Regulates the Variable Response to Diverse Stresses Open
The Mediator is composed of multiple subunits conserved from yeast to humans and plays a central role in transcription. The tail components are not required for basal transcription but are required for responses to different stresses. Whil…
The role of genotype in Saccharomyces cerevisiae resistance to 4-methylcyclohexanemethanol Open
The chemical 4-methylcyclohexanemethanol (MCHM) spilled in the Elk River near Charleston, WV in January 2014, impacting the drinking water of 300,000 residents. Initial studies on MCHM following the spill attempted to characterize its toxi…
ILE Strains Raw Reads and VCFs Open
Raw Illumina sequencing reads of evolved strains for (Ayers et al. in preparation) and Ayers PhD dissertation.Strains S1-S8 are control evolved strains.Strains S9-S16 are MCHM evolved strains.File names all contain S1-S16 as a strain ident…
View article: MCHM Acts as a Hydrotrope, Altering the Balance of Metals in Yeast
MCHM Acts as a Hydrotrope, Altering the Balance of Metals in Yeast Open
While drugs and other industrial chemicals are routinely studied to assess risks, many widely used chemicals have not been thoroughly evaluated. One such chemical, 4-methylcyclohexane methanol (MCHM), is an industrial coal-cleaning chemica…
View article: MCHM acts as a hydrotrope, altering the balance of metals in yeast
MCHM acts as a hydrotrope, altering the balance of metals in yeast Open
While drugs and other industrial chemicals are routinely studied to assess risks, many widely-used chemicals have not been thoroughly evaluated. One such chemical, 4-methylcyclohexane methanol (MCHM), is an industrial coal-cleaning chemica…
Transcriptional Profiling of <i>Saccharomyces cerevisiae</i> Reveals the Impact of Variation of a Single Transcription Factor on Differential Gene Expression in 4NQO, Fermentable, and Nonfermentable Carbon Sources Open
Cellular metabolism can change the potency of a chemical’s tumorigenicity. 4-nitroquinoline-1-oxide (4NQO) is a tumorigenic drug widely used on animal models for cancer research. Polymorphisms of the transcription factor Yrr1 confer differ…