Christian M. Harding
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View article: A multivalent capsule vaccine protects against Klebsiella pneumoniae bloodstream infections in healthy and immunocompromised mice
A multivalent capsule vaccine protects against Klebsiella pneumoniae bloodstream infections in healthy and immunocompromised mice Open
Klebsiella pneumoniae is a leading cause of nosocomial infections, bacteremia, and worldwide mortality. Further, a drastic rise in antibiotic-resistant isolates poses an urgent threat to humanity. Unfortunately, despite its clinical import…
View article: Differential virulence and immune recognition of <i>Klebsiella pneumoniae</i> O-antigen subtypes O2α and O2β
Differential virulence and immune recognition of <i>Klebsiella pneumoniae</i> O-antigen subtypes O2α and O2β Open
Klebsiella pneumoniae infections are sharply on the rise among at-risk populations. K. pneumoniae has nine serogroups of O-antigens. Recently, additional O-antigen subtypes within these serogroups have been identified; the contributions of…
View article: Enhancing O-linking oligosaccharyltransferase functionality through directed evolution
Enhancing O-linking oligosaccharyltransferase functionality through directed evolution Open
Polysaccharide protein conjugate vaccines consist of bacterial polysaccharides covalently linked to carrier proteins. Bioconjugate vaccines are a type of polysaccharide protein conjugate vaccine produced by oligosaccharyltransferases, whic…
View article: Antibody responses in Klebsiella pneumoniae bloodstream infection: a prospective cohort study
Antibody responses in Klebsiella pneumoniae bloodstream infection: a prospective cohort study Open
National Institute of Allergy and Infectious Diseases at the National Institutes of Health.
View article: Klebsiella pneumoniae bioconjugate vaccine functional durability in mice
Klebsiella pneumoniae bioconjugate vaccine functional durability in mice Open
View article: Antibody responses in<i>Klebsiella pneumoniae</i>bloodstream infection: a cohort study
Antibody responses in<i>Klebsiella pneumoniae</i>bloodstream infection: a cohort study Open
Summary Background Klebsiella pneumonia (Kpn) is the fourth leading cause of infection-related deaths globally, yet little is known about human antibody responses to invasive Kpn. In this study, we sought to determine whether the O-specifi…
View article: Heptavalent O-Antigen Bioconjugate Vaccine Exhibiting Differential Functional Antibody Responses Against Diverse <i>Klebsiella pneumoniae</i> Isolates
Heptavalent O-Antigen Bioconjugate Vaccine Exhibiting Differential Functional Antibody Responses Against Diverse <i>Klebsiella pneumoniae</i> Isolates Open
Klebsiella pneumoniae is the leading cause of neonatal sepsis and is increasingly difficult to treat owing to antibiotic resistance. Vaccination represents a tractable approach to combat this resistant bacterium; however, there is currentl…
View article: A heptavalent O-antigen bioconjugate vaccine exhibits differential functional antibody responses against diverse<i>Klebsiella pneumoniae</i>isolates
A heptavalent O-antigen bioconjugate vaccine exhibits differential functional antibody responses against diverse<i>Klebsiella pneumoniae</i>isolates Open
Klebsiella pneumoniae is a concerning pathogen that is now the leading cause of neonatal sepsis and is increasingly difficult to treat due to heightened antibiotic resistance. Thus, there is an urgent need for preventive and effective immu…
View article: Capsular polysaccharide inhibits vaccine-induced O-antigen antibody binding and function across both classical and hypervirulent K2:O1 strains of Klebsiella pneumoniae
Capsular polysaccharide inhibits vaccine-induced O-antigen antibody binding and function across both classical and hypervirulent K2:O1 strains of Klebsiella pneumoniae Open
Klebsiella pneumoniae presents as two circulating pathotypes: classical K . pneumoniae (c Kp) and hypervirulent K . pneumoniae (hv Kp ). Classical isolates are considered urgent threats due to their antibiotic resistance profiles, while hv…
View article: Capsular polysaccharide inhibits vaccine-induced O-antigen antibody binding and function across both classical and hypervirulent K2:O1 strains of<i>Klebsiella pneumoniae</i>
Capsular polysaccharide inhibits vaccine-induced O-antigen antibody binding and function across both classical and hypervirulent K2:O1 strains of<i>Klebsiella pneumoniae</i> Open
Klebsiella pneumoniae presents as two circulating pathotypes: classical K. pneumoniae (c Kp ) and hypervirulent K. pneumoniae (hv Kp ). Classical isolates are considered urgent threats due to their antibiotic resistance profiles, while hv …
View article: Discovery and characterization of a new class of <i>O</i>-linking oligosaccharyltransferases from the <i>Moraxellaceae</i> family
Discovery and characterization of a new class of <i>O</i>-linking oligosaccharyltransferases from the <i>Moraxellaceae</i> family Open
Bacterial protein glycosylation is commonly mediated by oligosaccharyltransferases (OTases) that transfer oligosaccharides en bloc from preassembled lipid-linked precursors to acceptor proteins. Natively, O-linking OTases usually transfer …
View article: Immunogenicity and protective efficacy of a prototype pneumococcal bioconjugate vaccine
Immunogenicity and protective efficacy of a prototype pneumococcal bioconjugate vaccine Open
View article: Development and Immunogenicity of a Prototype Multivalent Group B <i>Streptococcus</i> Bioconjugate Vaccine
Development and Immunogenicity of a Prototype Multivalent Group B <i>Streptococcus</i> Bioconjugate Vaccine Open
Group B Streptococcus (GBS) is a leading cause of neonatal infections and invasive diseases in nonpregnant adults worldwide. Developing a protective conjugate vaccine targeting the capsule of GBS has been pursued for more than 30 ye…
View article: A minimal sequon sufficient for <i>O</i>-linked glycosylation by the versatile oligosaccharyltransferase PglS
A minimal sequon sufficient for <i>O</i>-linked glycosylation by the versatile oligosaccharyltransferase PglS Open
Bioconjugate vaccines, consisting of polysaccharides attached to carrier proteins, are enzymatically generated using prokaryotic glycosylation systems in a process termed bioconjugation. Key to bioconjugation are a group of enzymes known a…
View article: A promising bioconjugate vaccine against hypervirulent <i>Klebsiella pneumoniae</i>
A promising bioconjugate vaccine against hypervirulent <i>Klebsiella pneumoniae</i> Open
Significance Klebsiella pneumoniae is considered a nosocomial pathogen, usually infecting immunocompromised patients. However, a pathotype of K. pneumoniae , termed hypervirulent K. pneumoniae (hv Kp ), has emerged and is spreading through…
View article: Glycoengineering bioconjugate vaccines, therapeutics, and diagnostics in E. coli
Glycoengineering bioconjugate vaccines, therapeutics, and diagnostics in E. coli Open
The first, general glycosylation pathway in bacteria, the N-linked glycosylation system of Campylobacter jejuni, was discovered two decades ago. Since then, many diverse prokaryotic glycosylation systems have been characterized, including …
View article: A platform for glycoengineering a polyvalent pneumococcal bioconjugate vaccine using E. coli as a host
A platform for glycoengineering a polyvalent pneumococcal bioconjugate vaccine using E. coli as a host Open
View article: Distinct amino acid residues confer one of three UDP-sugar substrate specificities in Acinetobacter baumannii PglC phosphoglycosyltransferases
Distinct amino acid residues confer one of three UDP-sugar substrate specificities in Acinetobacter baumannii PglC phosphoglycosyltransferases Open
Acinetobacter baumannii is an opportunistic human pathogen with the highest reported rates of multidrug resistance among Gram-negative pathogens. The capsular polysaccharide of A. baumannii is considered one of its most significant virulen…
View article: Pathogenic Acinetobacter species have a functional type I secretion system and contact-dependent inhibition systems
Pathogenic Acinetobacter species have a functional type I secretion system and contact-dependent inhibition systems Open
Pathogenic Acinetobacter species, including Acinetobacter baumannii and Acinetobacter nosocomialis, are opportunistic human pathogens of increasing relevance worldwide. Although their mechanisms of drug resistance are …
View article: Structural Diversity in the Type IV Pili of Multidrug-resistant Acinetobacter
Structural Diversity in the Type IV Pili of Multidrug-resistant Acinetobacter Open
View article: Characteristics of invasive Acinetobacter species isolates recovered in a pediatric academic center
Characteristics of invasive Acinetobacter species isolates recovered in a pediatric academic center Open
View article: KatG and KatE confer Acinetobacter resistance to hydrogen peroxide but sensitize bacteria to killing by phagocytic respiratory burst
KatG and KatE confer Acinetobacter resistance to hydrogen peroxide but sensitize bacteria to killing by phagocytic respiratory burst Open
View article: Medically Relevant Acinetobacter Species Require a Type II Secretion System and Specific Membrane-Associated Chaperones for the Export of Multiple Substrates and Full Virulence
Medically Relevant Acinetobacter Species Require a Type II Secretion System and Specific Membrane-Associated Chaperones for the Export of Multiple Substrates and Full Virulence Open
Acinetobacter baumannii, A. nosocomialis, and A. pittii have recently emerged as opportunistic human pathogens capable of causing severe human disease; however, the molecular mechanisms employed by Acinetobacter to cause disease remain poo…
View article: Pathogenic Acinetobacter: from the Cell Surface to Infinity and Beyond
Pathogenic Acinetobacter: from the Cell Surface to Infinity and Beyond Open
The genus Acinetobacter encompasses multiple nosocomial opportunistic pathogens that are of increasing worldwide relevance because of their ability to survive exposure to various antimicrobial and sterilization agents. Among these, Acineto…
View article: <scp><i>A</i></scp><i>cinetobacter</i> strains carry two functional oligosaccharyltransferases, one devoted exclusively to type <scp>IV</scp> pilin, and the other one dedicated to <scp><i>O</i></scp>‐glycosylation of multiple proteins
<span><i>A</i></span><i>cinetobacter</i> strains carry two functional oligosaccharyltransferases, one devoted exclusively to type <span>IV</span> pilin, and the other one dedicated to <span><i>O</i></span>‐glycosylation of multiple proteins Open
Summary Multiple species within the A cinetobacter genus are nosocomial opportunistic pathogens of increasing relevance worldwide. Among the virulence factors utilized by these bacteria are the type IV pili and a protein O ‐glycosylation s…