Michael D. Flythe
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View article: Application of Hops (Humulus lupulus L.) and β-Acid Extract to Improve Aerobic Stability and In Vitro Ruminal Fermentation of Maralfalfa Grass Silage
Application of Hops (Humulus lupulus L.) and β-Acid Extract to Improve Aerobic Stability and In Vitro Ruminal Fermentation of Maralfalfa Grass Silage Open
The potential of hops (Humulus lupulus L.) and β-acid extract were evaluated for improving the quality of maralfalfa grass (Cenchrus purpureus) silage (with added sorghum grain, sorghum straw, and urea) during aerobic exposure and their re…
View article: 16 Comparison of sheep and cattle rumen microbiological responses to an abrupt transition from a high-forage to a high-concentrate diet
16 Comparison of sheep and cattle rumen microbiological responses to an abrupt transition from a high-forage to a high-concentrate diet Open
Sheep and cattle are both ruminant species; however, previous in vitro studies have indicated potential species differences in rumen carbohydrate utilization and acidosis susceptibility. To investigate this in vivo, the objective of this s…
View article: Effects of phyto-phenolic compounds on ammonia production by select amino acid fermenting bacteria
Effects of phyto-phenolic compounds on ammonia production by select amino acid fermenting bacteria Open
Bacteria that ferment amino acids to ammonia can be categorized as generalists or specialist hyper-ammonia-producing bacteria. In the rumens of ruminant animals, most of the ammonia produced is eventually excreted as urea in urine. This pr…
View article: Effects of isoflavone supplementation, via red clover hay, on the growth and postgraze physiological recovery of beef steers grazing endophyte-infected tall fescue pastures
Effects of isoflavone supplementation, via red clover hay, on the growth and postgraze physiological recovery of beef steers grazing endophyte-infected tall fescue pastures Open
Livestock grazing endophyte-infected (E+) tall fescue can exhibit persistent systemic vasoconstriction and fescue toxicosis. Isoflavones in legumes, most notably red clover (RC), are known hypotensive agents. The objective of the experimen…
View article: Biochanin A feed supplementation alters dynamics of trace gas emissions from lamb urine‐amended soil
Biochanin A feed supplementation alters dynamics of trace gas emissions from lamb urine‐amended soil Open
Sustainable growth in livestock production requires reductions in trace gas emissions on grazing lands. Urine excreta patches are hot spots for accelerated emissions of carbon and nitrogen. Ruminant dietary supplementation with the isoflav…
View article: PSIV-11 The inhibitory activity of phyto-phenolic compounds on ammonia-producing ruminal bacteria
PSIV-11 The inhibitory activity of phyto-phenolic compounds on ammonia-producing ruminal bacteria Open
Antibiotic resistance has progressively increased over time, thus necessitating the discovery of sustainable, novel antimicrobials. Ammonia producing bacteria (APB) are predominant organisms occupying many niches in the rumens of cattle an…
View article: PSI-19 Functional rumen microbiological characteristics of conventionally versus artificially reared triplet Polypay lambs from birth to weaning
PSI-19 Functional rumen microbiological characteristics of conventionally versus artificially reared triplet Polypay lambs from birth to weaning Open
Artificial rearing remains an integral practice in sheep production largely due to the unique prolificacy of ewes as opposed to other species. Research has demonstrated slower growth and reduced performance of lambs reared on milk replacer…
View article: Microbial-Derived Exerkines Prevent Skeletal Muscle Atrophy
Microbial-Derived Exerkines Prevent Skeletal Muscle Atrophy Open
Regular exercise yields a multitude of systemic benefits, many of which may be mediated through the gut microbiome. Here, we report that cecal microbial transplants (CMTs) from exercise-trained vs. sedentary mice have modest benefits in re…
View article: Activity of Cannabidiol on Ex Vivo Amino Acid Fermentation by Bovine Rumen Microbiota
Activity of Cannabidiol on Ex Vivo Amino Acid Fermentation by Bovine Rumen Microbiota Open
Amino-acid-fermenting bacteria are wasteful organisms within the rumens of beef cattle that remove dietary amino nitrogen by producing ammonia, which is then excreted renally. There are currently no on-label uses for the control of this mi…
View article: Antimicrobial effects of cannabidiol on select agriculturally important Clostridia
Antimicrobial effects of cannabidiol on select agriculturally important Clostridia Open
Amino acid-fermenting Clostridia have undesirable effects in agricultural systems, which can be mitigated by antibiotics, but resistance necessitates alternatives. Here, we demonstrate the efficacy of cannabidiol on growth and ammonia inhi…
View article: 87 Functional rumen microbiological survey of triplet-bearing Polypay ewes from parturition to weaning
87 Functional rumen microbiological survey of triplet-bearing Polypay ewes from parturition to weaning Open
Ewes transitioning from late gestation to early lactation face a number of challenges including physiological stress, dietary changes, and metabolic adaptations, particularly when bearing and rearing multiple offspring. Furthermore, rumen …
View article: 127 Color and oxidative stability of Longissimus lumborum muscles from artificially raised ram lambs finished on a high-concentrate diet
127 Color and oxidative stability of Longissimus lumborum muscles from artificially raised ram lambs finished on a high-concentrate diet Open
Pre-weaning strategies, such as artificially raising on milk replacer, can impact lamb quality. Limited studies have examined the influence of artificial raising on color attributes of lamb. The aim of this study was to evaluate how artifi…
View article: 48 The ruminal microbiome of conventionally and artificially raised lambs fed high-concentrate and high-forage post-weaning diets
48 The ruminal microbiome of conventionally and artificially raised lambs fed high-concentrate and high-forage post-weaning diets Open
Artificially rearing lambs on milk replacer impacts development of the ruminal microbiome. The objective was to evaluate the extent to which pre-weaning management influences the ruminal microbiome of lambs during finishing. A finishing st…
View article: 93 Red clover as a functional feed: Effects on rumen microbiota of finishing ram lambs
93 Red clover as a functional feed: Effects on rumen microbiota of finishing ram lambs Open
Red clover (RC) contains bioactive isoflavones. Supplementing RC as a functional feed (as little as 7.5% of the total diet) has been shown to alter ruminal fermentation and improve feed efficiency in ram lambs fed high-concentrate finishin…
View article: Growth and Metabolism of Clostridioides difficile in Hungate-Style Media
Growth and Metabolism of Clostridioides difficile in Hungate-Style Media Open
Clostridioides difficile is a clinically and agriculturally important organism with diverse metabolic capabilities. Commercially available media types to cultivate C. difficile typically include multiple growth substrates and often selecti…
View article: Ex Vivo Fermentation of Hay and Corn by Rumen Bacteria from Cattle and Sheep
Ex Vivo Fermentation of Hay and Corn by Rumen Bacteria from Cattle and Sheep Open
Sheep are often utilized as a model ruminant, despite a lack of functional comparisons of rumen bacterial communities and responses during dietary transitions between sheep and cattle. Therefore, an ex vivo study was conducted to evaluate …
View article: Fructan Catabolism by Rumen Microbiota of Cattle and Sheep
Fructan Catabolism by Rumen Microbiota of Cattle and Sheep Open
Fructans serve as the primary form of storage carbohydrate in cool-season grasses, but little is known about potential differences in ruminal fermentation of fructans between cattle and sheep. An ex vivo study was conducted to evaluate spe…
View article: The Establishment of Fibrolytic Bacteria in the Foal Gastrointestinal Tract Is Related to the Occurrence of Coprophagy by Foals
The Establishment of Fibrolytic Bacteria in the Foal Gastrointestinal Tract Is Related to the Occurrence of Coprophagy by Foals Open
The consumption of maternal feces (coprophagy) is commonly observed in healthy foals and is a proposed contributor to microbial colonization of the foal’s gastrointestinal tract (GIT). This study investigated the role of coprophagy in the …
View article: Editorial: Natural products in animal feed and production systems
Editorial: Natural products in animal feed and production systems Open
EDITORIAL article Front. Anim. Sci., 01 May 2023Sec. Animal Nutrition Volume 4 - 2023 | https://doi.org/10.3389/fanim.2023.1204663
View article: 51 Use of Plant Secondary Metabolites and Impact on Beef Cattle Production
51 Use of Plant Secondary Metabolites and Impact on Beef Cattle Production Open
The objective of the presentation is to describe the beneficial roles of plant secondary metabolites in beef production with special attention to mode of delivery. Plant and fungal secondary metabolites are responsible for many well-known …
View article: 91<i>Ex Vivo</i> Fermentation of Corn and hay by Rumen Bacteria from Sheep and Cattle
91<i>Ex Vivo</i> Fermentation of Corn and hay by Rumen Bacteria from Sheep and Cattle Open
Data from cattle and sheep studies are often utilized interchangeably to make inferences regarding ruminant nutrition and physiology, but few studies have directly compared rumen microbiological responses. The objective of this study was t…
View article: Reduction in Rumen Tetracycline-Insensitive Bacteria during a Grain Challenge Using the Isoflavone Biochanin A
Reduction in Rumen Tetracycline-Insensitive Bacteria during a Grain Challenge Using the Isoflavone Biochanin A Open
The isoflavone biochanin A was previously shown to promote weight gain in growing steers by selectively inhibiting rumen bacteria-like growth-promoting feed antibiotics. The hypothesis that biochanin A inhibited the action of drug efflux p…
View article: Effects of Hops Treatment on Nitrogen Retention, Volatile Fatty Acid Accumulations, and Select Microbial Populations of Composting Poultry Litter Intended for Use as a Ruminant Feedstuff
Effects of Hops Treatment on Nitrogen Retention, Volatile Fatty Acid Accumulations, and Select Microbial Populations of Composting Poultry Litter Intended for Use as a Ruminant Feedstuff Open
Poultry litter is a valuable crude protein feedstuff for ruminants, but it must be treated to kill pathogens before feeding. Composting effectively kills pathogens, but it risks losing ammonia to volatilization or leaching during degradati…
View article: Profiling of cool-season forage arabinoxylans via a validated HPAEC-PAD method
Profiling of cool-season forage arabinoxylans via a validated HPAEC-PAD method Open
Cool-season pasture grasses contain arabinoxylans (AX) as their major cell wall hemicellulosic polysaccharide. AX structural differences may influence enzymatic degradability, but this relationship has not been fully explored in the AX fro…
View article: Red clover supplementation modifies rumen fermentation and promotes feed efficiency in ram lambs
Red clover supplementation modifies rumen fermentation and promotes feed efficiency in ram lambs Open
Red clover produces isoflavones, including biochanin A, which have been shown to have microbiological effects on the rumen while also promoting growth in beef cattle. The objective was to determine if supplementation of biochanin A via red…
View article: Conventional loose mineral with added red clover leaf (Trifolium pratense L.) reverses vasoconstriction associated with tall fescue toxicosis in steers
Conventional loose mineral with added red clover leaf (Trifolium pratense L.) reverses vasoconstriction associated with tall fescue toxicosis in steers Open
Endophyte-infected tall fescue (E + TF) can cause persistent vasoconstriction in ruminants and consequently fescue toxicosis. Legumes, including red clover (RC), contain isoflavones that are known hypotensive agents. Previous experiments h…
View article: PSVII-5 Effects of Hops Treatment on Nitrogen Retention of Composted Poultry Litter and Subsequent Rumen Fermentation
PSVII-5 Effects of Hops Treatment on Nitrogen Retention of Composted Poultry Litter and Subsequent Rumen Fermentation Open
We tested the effects of Galena or Chinook hops treatment (6% wt/wt) of simulated composts (13 d) of wood chip litter (WCL) alone or mixed 3:1 with ground Bluestem hay (BSH). Results revealed that net losses of urea and ammonia in the WCL …