Michael Dal Molin
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View article: Protocol to quantify bacterial burden in time-kill assays using colony-forming units and most probable number readouts for Mycobacterium tuberculosis
Protocol to quantify bacterial burden in time-kill assays using colony-forming units and most probable number readouts for Mycobacterium tuberculosis Open
Here, we present a protocol to perform a time-kill assay (TKA) to quantify bacterial burden at multiple time points using colony-forming units and most probable number readouts simultaneously. We describe steps for preparing inoculum, expe…
View article: Development of 2nd generation aminomethyl spectinomycins that overcome native efflux in<i>Mycobacterium</i>abscessus
Development of 2nd generation aminomethyl spectinomycins that overcome native efflux in<i>Mycobacterium</i>abscessus Open
Mycobacterium abscessus ( Mab ), a nontuberculous mycobacterial (NTM) species, is an emerging pathogen with high intrinsic drug resistance. Current standard-of-care therapy results in poor outcomes, demonstrating the urgent need to develop…
View article: Characterization of Two Novel Inhibitors of the Mycobacterium tuberculosis Cytochrome <i>bc</i> <sub>1</sub> Complex
Characterization of Two Novel Inhibitors of the Mycobacterium tuberculosis Cytochrome <i>bc</i> <sub>1</sub> Complex Open
Drug-resistant tuberculosis is a global health care threat calling for novel effective treatment options. Here, we report on two novel cytochrome bc 1 inhibitors (MJ-22 and B6) targeting the Mycobacterium tuberculosis respiratory chain wit…
View article: A Reinvestigation of the Role of the Sorbic Acid Tail on the Antibacterial and Anti‐Tuberculosis Properties of Moiramide B
A Reinvestigation of the Role of the Sorbic Acid Tail on the Antibacterial and Anti‐Tuberculosis Properties of Moiramide B Open
Due to worldwide increasing resistances, there is a considerable need for antibacterial compounds with modes of action not yet realized in commercial antibiotics. One such promising structure is the acetyl‐CoA carboxylase (ACC) inhibitor m…
View article: Increased drug permeability of a stiffened mycobacterial outer membrane in cells lacking MFS transporter Rv1410 and lipoprotein LprG
Increased drug permeability of a stiffened mycobacterial outer membrane in cells lacking MFS transporter Rv1410 and lipoprotein LprG Open
Summary The major facilitator superfamily transporter Rv1410 and the lipoprotein LprG (Rv1411) are encoded by a conserved two‐gene operon and contribute to virulence in Mycobacterium tuberculosis . Rv1410 was originally postulated to funct…
View article: Lipoprotein Glycosylation by Protein-O-Mannosyltransferase (MAB_1122c) Contributes to Low Cell Envelope Permeability and Antibiotic Resistance of Mycobacterium abscessus
Lipoprotein Glycosylation by Protein-O-Mannosyltransferase (MAB_1122c) Contributes to Low Cell Envelope Permeability and Antibiotic Resistance of Mycobacterium abscessus Open
Lipoproteins are important components of the mycobacterial cell envelope due to their function in cell wall homeostasis and bacterial virulence. They are post-translationally modified with lipid- and glycosyl-residues in various species an…
View article: Molecular Mechanisms of Intrinsic Streptomycin Resistance in Mycobacterium abscessus
Molecular Mechanisms of Intrinsic Streptomycin Resistance in Mycobacterium abscessus Open
Streptomycin, the first drug used for the treatment of tuberculosis, shows limited activity against the highly resistant pathogen Mycobacterium abscessus . We recently identified two aminoglycoside-acetylating genes [ aac(2′) and eis2 ] wh…
View article: Elucidation of Mycobacterium abscessus aminoglycoside and capreomycin resistance by targeted deletion of three putative resistance genes
Elucidation of Mycobacterium abscessus aminoglycoside and capreomycin resistance by targeted deletion of three putative resistance genes Open
M. abscessus expresses two distinct AG resistance determinants, AAC(2 ' ) and Eis2, which confer clinically relevant drug resistance.