Michael E. Webb
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View article: Discovery and biosynthesis of biffamycin A – a novel glycotetrapeptide antibiotic
Discovery and biosynthesis of biffamycin A – a novel glycotetrapeptide antibiotic Open
The clinical deployment of antibiotics is undermined by antimicrobial resistance. Without new agents to treat antibiotic resistant bacterial infections, mortality rates are predicted to reach 10 million people per year by 2050. Most antibi…
View article: Membrane Transport Modulates the pH-Regulated Feedback of an Enzyme Reaction Confined within Lipid Vesicles
Membrane Transport Modulates the pH-Regulated Feedback of an Enzyme Reaction Confined within Lipid Vesicles Open
Understanding ion transport dynamics in reactive vesicles is pivotal for exploring biological and chemical processes and essential for designing synthetic cells. In this work, we investigate how proton transport and membrane potential regu…
View article: Generating and validating renewable affimer protein binding reagents targeting SH2 domains
Generating and validating renewable affimer protein binding reagents targeting SH2 domains Open
Despite SH2 domains, being pivotal in protein interactions linked to various diseases like cancer, we lack specific research tools for intracellular assays. Understanding SH2-mediated interactions and creating effective inhibitors requires…
View article: Mapping Co-benefits and Cultural values for Nature-based Solutions: Caye Caulker, Belize (report no. 8)
Mapping Co-benefits and Cultural values for Nature-based Solutions: Caye Caulker, Belize (report no. 8) Open
On October 21st and 22nd, 2024, 58 persons gathered in Caye Caulker for a collaborative workshop, sharing knowledge on priority areas for Nature-based Solutions (NbS) and co-benefits pertaining to mangroves and coral reefs. They also helpe…
View article: Sortase-Modified Cholera Toxoids Show Specific Golgi Localization
Sortase-Modified Cholera Toxoids Show Specific Golgi Localization Open
Cholera toxoid is an established tool for use in cellular tracing in neuroscience and cell biology. We use a sortase labeling approach to generate site-specific N-terminally modified variants of both the A2-B5 heterohexamer and B5 pentamer…
View article: High-Throughput profiling of SH2 domains using Affimer reagents: unravelling protein interaction networks
High-Throughput profiling of SH2 domains using Affimer reagents: unravelling protein interaction networks Open
Despite SH2 domains, being pivotal in protein interactions linked to various diseases like cancer, we lack specific research tools for intracellular assays. Understanding SH2-mediated interactions and creating effective inhibitors requires…
View article: The Mutagenic Plasticity of the Cholera Toxin B-Subunit Surface Residues: Stability and Affinity
The Mutagenic Plasticity of the Cholera Toxin B-Subunit Surface Residues: Stability and Affinity Open
Mastering selective molecule trafficking across human cell membranes poses a formidable challenge in healthcare biotechnology while offering the prospect of breakthroughs in drug delivery, gene therapy, and diagnostic imaging. The cholera …
View article: Quantitative N‐ or C‐Terminal Labelling of Proteins with Unactivated Peptides by Use of Sortases and a <scp>d</scp>‐Aminopeptidase
Quantitative N‐ or C‐Terminal Labelling of Proteins with Unactivated Peptides by Use of Sortases and a <span>d</span>‐Aminopeptidase Open
Quantitative and selective labelling of proteins is widely used in both academic and industrial laboratories, and catalytic labelling of proteins using transpeptidases, such as sortases, has proved to be a popular strategy for such selecti…
View article: Quantitative N‐ or C‐Terminal Labelling of Proteins with Unactivated Peptides by Use of Sortases and a <scp>d</scp>‐Aminopeptidase
Quantitative N‐ or C‐Terminal Labelling of Proteins with Unactivated Peptides by Use of Sortases and a <span>d</span>‐Aminopeptidase Open
Quantitative and selective labelling of proteins is widely used in both academic and industrial laboratories, and catalytic labelling of proteins using transpeptidases, such as sortases, has proved to be a popular strategy for such selecti…
View article: A ‘glyco-fluorine’ code revealing differential recognition by glycan binding partners
A ‘glyco-fluorine’ code revealing differential recognition by glycan binding partners Open
Biosensing or diagnostics using glycan sequences as targets is limited by glycan cross-reactivities. As binding sites of different proteins that all recognise a given glycan will not be identical, we introduce application of a library of s…
View article: Membrane Fusion Mediated by Non-covalent Binding of Re-engineered Cholera Toxin Assemblies to Glycolipids
Membrane Fusion Mediated by Non-covalent Binding of Re-engineered Cholera Toxin Assemblies to Glycolipids Open
Membrane fusion is essential for the transport of macromolecules and viruses across membranes. While glycan-binding proteins (lectins) often initiate cellular adhesion, subsequent fusion events require additional protein machinery. No mech…
View article: Combined Application of Orthogonal Sortases and Depsipeptide Substrates for Dual Protein Labeling
Combined Application of Orthogonal Sortases and Depsipeptide Substrates for Dual Protein Labeling Open
Staphylococcus aureus sortase A is a transpeptidase that has been extensively exploited for site-specific modification of proteins and was originally used to attach a labeling reagent containing an LPXTG recognition sequence to a protein o…
View article: Synthesis of cholera toxin B subunit glycoconjugates using site-specific orthogonal oxime and sortase ligation reactions
Synthesis of cholera toxin B subunit glycoconjugates using site-specific orthogonal oxime and sortase ligation reactions Open
The chemoenzymatic synthesis of a series of dual N- and C-terminal–functionalized cholera toxin B subunit (CTB) glycoconjugates is described. Mucin 1 peptides bearing different levels of Tn antigen glycosylation [MUC1(Tn)] were prepared vi…
View article: Bioorthogonal, Bifunctional Linker for Engineering Synthetic Glycoproteins
Bioorthogonal, Bifunctional Linker for Engineering Synthetic Glycoproteins Open
Post-translational glycosylation of proteins results in complex mixtures of heterogeneous protein glycoforms. Glycoproteins have many potential applications from fundamental studies of glycobiology to potential therapeutics, but generating…
View article: In-Depth Characterization of a Re-Engineered Cholera Toxin Manufacturing Process Using Growth-Decoupled Production in Escherichia coli
In-Depth Characterization of a Re-Engineered Cholera Toxin Manufacturing Process Using Growth-Decoupled Production in Escherichia coli Open
Non-toxic derivatives of the cholera toxin are extensively used in neuroscience, as neuronal tracers to reveal the location of cells in the central nervous system. They are, also, being developed as vaccine components and drug-delivery veh…
View article: Machine Learning on a Robotic Platform for the Design of Polymer–Protein Hybrids
Machine Learning on a Robotic Platform for the Design of Polymer–Protein Hybrids Open
Polymer–protein hybrids are intriguing materials that can bolster protein stability in non‐native environments, thereby enhancing their utility in diverse medicinal, commercial, and industrial applications. One stabilization strategy invol…
View article: The N-terminal substrate specificity of the SurE peptide cyclase
The N-terminal substrate specificity of the SurE peptide cyclase Open
The N-terminal substrate specificity of the SurE peptide cyclase was elucidated using a combination of on-resin biomemtic substrates and conventional SNAC thioesters.
View article: Challenges in the use of sortase and other peptide ligases for site-specific protein modification
Challenges in the use of sortase and other peptide ligases for site-specific protein modification Open
We highlight chemical and biochemical strategies taken to optimise peptide and protein modification using peptide ligases.
View article: Membrane fusion mediated by non-covalent binding of re-engineered cholera toxin assemblies to glycolipids
Membrane fusion mediated by non-covalent binding of re-engineered cholera toxin assemblies to glycolipids Open
Membrane fusion is essential for the transport of macromolecules and viruses across membranes. While glycan-binding proteins (lectins) often initiate cellular adhesion, subsequent fusion events require additional protein machinery. No mech…
View article: Membrane fusion mediated by non-covalent binding of re-engineered cholera toxin assemblies to glycolipids
Membrane fusion mediated by non-covalent binding of re-engineered cholera toxin assemblies to glycolipids Open
Membrane fusion is essential for the transport of macromolecules and viruses across membranes. While glycan-binding proteins (lectins) often initiate cellular adhesion, subsequent fusion events require additional protein machinery. No mech…
View article: A Standalone β-Ketoreductase Acts Concomitantly with Biosynthesis of the Antimycin Scaffold
A Standalone β-Ketoreductase Acts Concomitantly with Biosynthesis of the Antimycin Scaffold Open
Antimycins are anticancer compounds produced by a hybrid nonribosomal peptide synthetase/polyketide synthase (NRPS/PKS) pathway. The biosynthesis of these compounds is well characterized, with the exception of the standalone β-ketoreductas…
View article: The Desotamide Family of Antibiotics
The Desotamide Family of Antibiotics Open
Microbial natural products underpin the majority of antimicrobial compounds in clinical use and the discovery of new effective antibacterial treatments is urgently required to combat growing antimicrobial resistance. Non-ribosomal peptides…
View article: Piggybacking on the Cholera Toxin: Identification of a Toxoid-binding Protein as an Approach for Targeted Delivery of Proteins to Motor Neurons
Piggybacking on the Cholera Toxin: Identification of a Toxoid-binding Protein as an Approach for Targeted Delivery of Proteins to Motor Neurons Open
A significant unmet need exists for the delivery of biologic drugs such as polypeptides or nucleic acids, to the central nervous system (CNS) for the treatment and understanding of neurodegenerative diseases. Naturally occurring toxoids ha…
View article: A versatile cholera toxin conjugate for neuronal targeting and tracing
A versatile cholera toxin conjugate for neuronal targeting and tracing Open
A novel azido-modified cholera toxin B-subunit has been developed for use in vivo as a neuronal tracer.
View article: Sortase-Modified Cholera Toxoids Show Specific Golgi Localization
Sortase-Modified Cholera Toxoids Show Specific Golgi Localization Open
Cholera toxoid is an established tool for use in cellular tracing in neuroscience and cell biology. We use a sortase-labelling approach to generate site-specifically N -terminally modified variants of both the A2-B 5 heterohexamer and B 5 …
View article: Directed Assembly of Homopentameric Cholera Toxin B-Subunit Proteins into Higher-Order Structures Using Coiled-Coil Appendages
Directed Assembly of Homopentameric Cholera Toxin B-Subunit Proteins into Higher-Order Structures Using Coiled-Coil Appendages Open
The self-assembly of proteins into higher order structures is ubiquitous in living systems. It is also an essential process for the bottom-up creation of novel molecular architectures and devices for synthetic biology. However, the complex…
View article: Chain termination of structurally disparate non-ribosomal peptides by a trans-acting β-lactamase
Chain termination of structurally disparate non-ribosomal peptides by a trans-acting β-lactamase Open
Non-ribosomal peptide synthetases (NRPSs) are responsible for the natural in vivo production of a large number of therapeutically relevant compounds. The non-ribosomal peptides (NRPs) formed by their action are the product of the long modu…
View article: CCDC 1889089: Experimental Crystal Structure Determination
CCDC 1889089: Experimental Crystal Structure Determination Open
An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available …
View article: A catalytic protein–proteomimetic complex: using aromatic oligoamide foldamers as activators of RNase S
A catalytic protein–proteomimetic complex: using aromatic oligoamide foldamers as activators of RNase S Open
An aromatic oligoamide foldamer acts as an α-helix mimetic and binds to the RNase S-protein resulting in restoration of its catalytic function.