Michael Eckart
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View article: Figure S3 from Defining the Antitumor Mechanism of Action of a Clinical-stage Compound as a Selective Degrader of the Nuclear Pore Complex
Figure S3 from Defining the Antitumor Mechanism of Action of a Clinical-stage Compound as a Selective Degrader of the Nuclear Pore Complex Open
Shows transcriptomic and protein analyses demonstrating TRIM21-dependent NFκB activation by PRLX, including mRNA-seq, GSEA, confocal imaging, and subcellular fractionation immunoblots.
View article: Supplementary Methods S1 from Defining the Antitumor Mechanism of Action of a Clinical-stage Compound as a Selective Degrader of the Nuclear Pore Complex
Supplementary Methods S1 from Defining the Antitumor Mechanism of Action of a Clinical-stage Compound as a Selective Degrader of the Nuclear Pore Complex Open
Chemical synthesis methods.
View article: Figure S11 from Defining the Antitumor Mechanism of Action of a Clinical-stage Compound as a Selective Degrader of the Nuclear Pore Complex
Figure S11 from Defining the Antitumor Mechanism of Action of a Clinical-stage Compound as a Selective Degrader of the Nuclear Pore Complex Open
Depicts live-cell imaging, lethal fraction analyses, and protein interaction/immunoblot assays demonstrating TRIM21-dependent PRLX-induced nucleoporin destabilization and rapid cancer cell death.
View article: Figure S10 from Defining the Antitumor Mechanism of Action of a Clinical-stage Compound as a Selective Degrader of the Nuclear Pore Complex
Figure S10 from Defining the Antitumor Mechanism of Action of a Clinical-stage Compound as a Selective Degrader of the Nuclear Pore Complex Open
Shows biophysical and biochemical assays, including SPR, GST pulldown, streptavidin pulldown, and TR-FRET, demonstrating compound-induced interactions between TRIM21 and NUP98, and a schematic of the molecular glue mechanism.
View article: Figure S12 from Defining the Antitumor Mechanism of Action of a Clinical-stage Compound as a Selective Degrader of the Nuclear Pore Complex
Figure S12 from Defining the Antitumor Mechanism of Action of a Clinical-stage Compound as a Selective Degrader of the Nuclear Pore Complex Open
Shows immunoblot and live-cell imaging analyses assessing washout-based cytotoxicity of PRLX and JWZ-8-103 in PANC-1 and hTERT RPE-1 cells.
View article: Figure S7 from Defining the Antitumor Mechanism of Action of a Clinical-stage Compound as a Selective Degrader of the Nuclear Pore Complex
Figure S7 from Defining the Antitumor Mechanism of Action of a Clinical-stage Compound as a Selective Degrader of the Nuclear Pore Complex Open
Shows proteomic and imaging analyses demonstrating TRIM21-dependent rapid degradation of nuclear pore complex proteins by PRLX and JWZ-8-103, including LC-MS/MS profiling, structural mapping, and confocal microscopy.
View article: Defining the Antitumor Mechanism of Action of a Clinical-stage Compound as a Selective Degrader of the Nuclear Pore Complex
Defining the Antitumor Mechanism of Action of a Clinical-stage Compound as a Selective Degrader of the Nuclear Pore Complex Open
Cancer cells are acutely dependent on nuclear transport due to elevated transcriptional activity, suggesting an unrealized opportunity for selective therapeutic inhibition of the nuclear pore complex (NPC). Through large-scale phenotypic p…
View article: A Bacterial Toxin Perturbs Intracellular Amino Acid Balance To Induce Persistence
A Bacterial Toxin Perturbs Intracellular Amino Acid Balance To Induce Persistence Open
To overcome various environmental challenges, bacterial cells can enter a physiologically quiescent state, known as dormancy or persistence, which balances growth and viability. In this study, we report a new mechanism by which a toxin-ant…
View article: A Localized Complex of Two Protein Oligomers Controls the Orientation of Cell Polarity
A Localized Complex of Two Protein Oligomers Controls the Orientation of Cell Polarity Open
Signaling hubs at bacterial cell poles establish cell polarity in the absence of membrane-bound compartments. In the asymmetrically dividing bacterium Caulobacter crescentus , cell polarity stems from the cell cycle-regulated localization …
View article: A cell cycle kinase with tandem sensory PAS domains integrates cell fate cues
A cell cycle kinase with tandem sensory PAS domains integrates cell fate cues Open
View article: Congo Red Interactions with Curli-Producing E. coli and Native Curli Amyloid Fibers
Congo Red Interactions with Curli-Producing E. coli and Native Curli Amyloid Fibers Open
Microorganisms produce functional amyloids that can be examined and manipulated in vivo and in vitro. Escherichia coli assemble extracellular adhesive amyloid fibers termed curli that mediate adhesion and promote biofi…