Michael Elbaum
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View article: Shadow montage and cone-beam reconstruction in 4D-STEM tomography
Shadow montage and cone-beam reconstruction in 4D-STEM tomography Open
Diffraction images in a scanning transmission electron microscope (STEM) provide a real-space projection of the sample at sufficient probe defocus. These so-called shadow images can be acquired patch by patch in a 4D-STEM setup using a pix…
View article: Contrast by electron microscopy in thick biological specimens
Contrast by electron microscopy in thick biological specimens Open
The contributions of coherent bright‐field phase and incoherent dark‐field amplitude contrast are investigated for thick biological specimens. A model for a T4 phage is constructed and images simulated for both TEM and STEM phase contrast …
View article: Serine-404 Phosphorylation and the R406W Modification in Tau Stabilize the cis-Proline Amide Bond, via Phosphoserine-Proline C–H/O and Proline-Aromatic C–H/π Interactions
Serine-404 Phosphorylation and the R406W Modification in Tau Stabilize the cis-Proline Amide Bond, via Phosphoserine-Proline C–H/O and Proline-Aromatic C–H/π Interactions Open
Tau misfolding, oligomerization, and aggregation are central to the pathology of Alzheimer's disease (AD), chronic traumatic encephalopathy (CTE), frontotemporal dementia, and other tauopathies. Increased phosphorylation of tau is associat…
View article: Structural effects of phosphorylation and beta-O-GlcNAcylation on alpha-helices and structural effects of phosphorylation and R406W on tau395-411
Structural effects of phosphorylation and beta-O-GlcNAcylation on alpha-helices and structural effects of phosphorylation and R406W on tau395-411 Open
The dynamic interplay between phosphorylation and beta-O-GlcNAcylation (OGlcNAc) of serine and threonine plays critical roles in numerous intracellular processes. Changes in phosphorylation and OGlcNAcylation are linked to Alzheimer's dise…
View article: Snapshots of mitochondrial fission imaged by cryo-scanning transmission electron tomography
Snapshots of mitochondrial fission imaged by cryo-scanning transmission electron tomography Open
Mitochondria undergo constant remodeling via fission, fusion, extension and degradation. Fission, in particular, depends on the accumulation of mitochondrial fission factor (MFF) and subsequent recruitment of the dynamin-related protein DR…
View article: Refractive index as an indicator for dynamic protein condensation in cell nuclei
Refractive index as an indicator for dynamic protein condensation in cell nuclei Open
Protein condensation is the basis for formation of membrane-less organelles in the cell. Most famously, weak, polyvalent interactions, often including RNA, may lead to a liquid-liquid phase separation. This effect greatly enhances local co…
View article: Silica biomineralization in plants alters the structure of lignin
Silica biomineralization in plants alters the structure of lignin Open
Lignin extracted from sorghum with native silicon levels have higher affinity to silicic acid as compared to lignin from low-silicon mutants. Silicic acid presence during lignin polymerization inhibits β-O-4 linkages on the expense of Si–O…
View article: Guanidinium Substitution Improves Self-Healing and Photodamage Resilience of MAPbI<sub>3</sub>
Guanidinium Substitution Improves Self-Healing and Photodamage Resilience of MAPbI<sub>3</sub> Open
Self-healing materials can become game changers for developing sustainable (opto)electronics. APbX3 halide (=X-) perovskites, HaPs, have shown a remarkable ability to self-heal damage. While we demonstrated self-healing in pure HaP compoun…
View article: Snapshots of Mitochondrial Fission Imaged by Cryo-Scanning Transmission Electron Tomography
Snapshots of Mitochondrial Fission Imaged by Cryo-Scanning Transmission Electron Tomography Open
Mitochondria undergo constant remodeling via fission, fusion, extension, and degradation. Fission, in particular, depends on the accumulation of the mitochondrial fission factor (MFF) and subsequent recruitment of the dynamin-related prote…
View article: Cryo-tomography and 3D Electron Diffraction Reveal the Polar Habit and Chiral Structure of the Malaria Pigment Crystal Hemozoin
Cryo-tomography and 3D Electron Diffraction Reveal the Polar Habit and Chiral Structure of the Malaria Pigment Crystal Hemozoin Open
Detoxification of heme in Plasmodium depends on its crystallization into hemozoin. This pathway is a major target of antimalarial drugs. The crystalline structure of hemozoin was established by X-ray powder diffraction using a synthetic an…
View article: Optimizing Contrast in Automated 4D STEM Cryotomography
Optimizing Contrast in Automated 4D STEM Cryotomography Open
4D STEM is an emerging approach to electron microscopy. While it was developed principally for high-resolution studies in materials science, the possibility to collect the entire transmitted flux makes it attractive for cryomicroscopy in a…
View article: Silica Biomineralization with Lignin Involves Si–O–C Bonds That Stabilize Radicals
Silica Biomineralization with Lignin Involves Si–O–C Bonds That Stabilize Radicals Open
Plants undergo substantial biomineralization of silicon, which is deposited primarily in cell walls as amorphous silica. The mineral formation could be moderated by the structure and chemistry of lignin, a polyphenol polymer that is a majo…
View article: Optimizing contrast in automated 4D-STEM cryo-tomography
Optimizing contrast in automated 4D-STEM cryo-tomography Open
4D-STEM is an emerging approach to electron microscopy. While it has been developed principally for high resolution studies in materials science, the possibility to collect the entire transmitted flux makes it attractive for cryo-microscop…
View article: Quantitative atomic cross section analysis by 4D-STEM and EELS
Quantitative atomic cross section analysis by 4D-STEM and EELS Open
We demonstrate the use of a 4-dimensional scanning transmission electron microscope (4D-STEM) to extract atomic cross section information in amorphous materials. We measure the scattering amplitudes of 200 keV electrons in several represen…
View article: Unraveling the multifaceted resilience of arsenic resistant bacterium Deinococcus indicus
Unraveling the multifaceted resilience of arsenic resistant bacterium Deinococcus indicus Open
Arsenic (As) is a toxic heavy metal widely found in the environment that severely undermines the integrity of water resources. Bioremediation of toxic compounds is an appellative sustainable technology with a balanced cost-effective setup.…
View article: Serine-404 Phosphorylation and the R406W Modification in Tau Stabilize the cis-Proline Amide Bond, via Phosphoserine-Proline C–H/O and Proline-Aromatic C–H/π Interactions
Serine-404 Phosphorylation and the R406W Modification in Tau Stabilize the cis-Proline Amide Bond, via Phosphoserine-Proline C–H/O and Proline-Aromatic C–H/π Interactions Open
Tau misfolding, oligomerization, and aggregation are central to the pathology of Alzheimer's disease (AD), chronic traumatic encephalopathy (CTE), frontotemporal dementia, and other tauopathies. Increased phosphorylation of tau is associat…
View article: Proline-Aromatic Sequences Stabilize Turns via C–H/π interactions in both cis-Proline and trans-Proline
Proline-Aromatic Sequences Stabilize Turns via C–H/π interactions in both cis-Proline and trans-Proline Open
In proteins, proline-aromatic sequences exhibit increased frequencies of cis-proline amide bonds, via proposed C–H/π interactions between the aromatic ring and either the proline ring or the backbone C–Hα of the residue prior to proline. T…
View article: Visualization of Organelles <em>In Situ</em> by Cryo-STEM Tomography
Visualization of Organelles <em>In Situ</em> by Cryo-STEM Tomography Open
Cryogenic electron microscopy (cryo-EM) relies on the imaging of biological or organic specimens embedded in their native aqueous medium; water is solidified into a glass (i.e., vitrified) without crystallization. The cryo-EM method is wid…
View article: A-Site Cation Dependence of Self-Healing in Polycrystalline APbI<sub>3</sub> Perovskite Films
A-Site Cation Dependence of Self-Healing in Polycrystalline APbI<sub>3</sub> Perovskite Films Open
In terms of sustainable use, halide perovskite (HaP) semiconductors have a strong advantage over most other classes of materials for (opto)electronics, as they can self-heal (SH) from photodamage. While there is considerable literature on …
View article: Topotactic, Vapor-Phase, <i>In Situ</i> Monitored Formation of Ultrathin, Phase-Pure 2D-on-3D Halide Perovskite Surfaces
Topotactic, Vapor-Phase, <i>In Situ</i> Monitored Formation of Ultrathin, Phase-Pure 2D-on-3D Halide Perovskite Surfaces Open
Two-dimensional (2D) halide perovskites, HaPs, can provide chemical stability to three-dimensional (3D) HaP surfaces, protecting them from exposure to ambient species and from reacting with contacting layers. Both actions occur with 2D HaP…
View article: 3DED/MicroED datasets of hemozoin recorded by a SINGLA detector
3DED/MicroED datasets of hemozoin recorded by a SINGLA detector Open
32 diffraction datasets were collected using a Thermo Scientific Glacios operated at 200 kV with a stage temperature of ~80 K. The gun lens was reduced to the minimum (8), and spot size (11) with a C2 aperture of 50 µm was used. The beam w…
View article: 3DED/MicroED datasets of hemozoin recorded by a SINGLA detector
3DED/MicroED datasets of hemozoin recorded by a SINGLA detector Open
32 diffraction datasets were collected using a Thermo Scientific Glacios operated at 200 kV with a stage temperature of ~80 K. The gun lens was reduced to the minimum (8), and spot size (11) with a C2 aperture of 50 µm was used. The beam w…
View article: Bridging the light-electron resolution gap with correlative cryo-SRRF and dual-axis cryo-STEM tomography
Bridging the light-electron resolution gap with correlative cryo-SRRF and dual-axis cryo-STEM tomography Open
Cryo-electron tomography (cryo-ET) is the prime method for cell biological studies in three dimensions (3D) at high resolution. We have introduced cryo-scanning transmission electron tomography (CSTET), which can access larger 3D volumes, …