Michael Forman
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View article: Enterovirus D68: Genomic and Clinical Comparison of 2 Seasons of Increased Viral Circulation and Discrepant Incidence of Acute Flaccid Myelitis—Maryland, USA
Enterovirus D68: Genomic and Clinical Comparison of 2 Seasons of Increased Viral Circulation and Discrepant Incidence of Acute Flaccid Myelitis—Maryland, USA Open
Background Enterovirus D68 (EV-D68) is associated with severe respiratory disease and acute flaccid myelitis (AFM). The 2022 outbreaks showed increased viral circulation and hospital admissions, but the expected rise in AFM cases did not o…
View article: Supplemental Figure 4 from Absence of Cytomegalovirus in Glioblastoma and Other High-grade Gliomas by Real-time PCR, Immunohistochemistry, and <i>In Situ</i> Hybridization
Supplemental Figure 4 from Absence of Cytomegalovirus in Glioblastoma and Other High-grade Gliomas by Real-time PCR, Immunohistochemistry, and <i>In Situ</i> Hybridization Open
False positive IHC staining in known negative control cells. IHC staining using the IE1 antibody (A). At the working dilution (1:8000) there is no positive staining in uninfected cells (bottom panel). At increased antibody concentrations (…
View article: Data from Absence of Cytomegalovirus in Glioblastoma and Other High-grade Gliomas by Real-time PCR, Immunohistochemistry, and <i>In Situ</i> Hybridization
Data from Absence of Cytomegalovirus in Glioblastoma and Other High-grade Gliomas by Real-time PCR, Immunohistochemistry, and <i>In Situ</i> Hybridization Open
Purpose: Reports of cytomegalovirus (CMV) detection in high-grade gliomas (HGG)/glioblastoma have been conflicting. We undertook a comprehensive approach to determine the presence or absence of CMV in tissue, plasma, and serum of HG…
View article: Supplemental Table 4 from Absence of Cytomegalovirus in Glioblastoma and Other High-grade Gliomas by Real-time PCR, Immunohistochemistry, and <i>In Situ</i> Hybridization
Supplemental Table 4 from Absence of Cytomegalovirus in Glioblastoma and Other High-grade Gliomas by Real-time PCR, Immunohistochemistry, and <i>In Situ</i> Hybridization Open
Studies that showed presence of CMV in gliomas versus studies that did not.
View article: Supplemental Figure 1 from Absence of Cytomegalovirus in Glioblastoma and Other High-grade Gliomas by Real-time PCR, Immunohistochemistry, and <i>In Situ</i> Hybridization
Supplemental Figure 1 from Absence of Cytomegalovirus in Glioblastoma and Other High-grade Gliomas by Real-time PCR, Immunohistochemistry, and <i>In Situ</i> Hybridization Open
CMV detection by CISH and IHC from CMV negative and positive cells with varying multiplicity of infection (M0I).
View article: Supplemental Figure 3 from Absence of Cytomegalovirus in Glioblastoma and Other High-grade Gliomas by Real-time PCR, Immunohistochemistry, and <i>In Situ</i> Hybridization
Supplemental Figure 3 from Absence of Cytomegalovirus in Glioblastoma and Other High-grade Gliomas by Real-time PCR, Immunohistochemistry, and <i>In Situ</i> Hybridization Open
IHC controls for tissue immunogenicity. IHC for vimentin (A) shows diffuse positivity and Ki-67 (B) shows widespread nuclear staining in a representative tumor sample. Original magnifications x 200.
View article: Data from Absence of Cytomegalovirus in Glioblastoma and Other High-grade Gliomas by Real-time PCR, Immunohistochemistry, and <i>In Situ</i> Hybridization
Data from Absence of Cytomegalovirus in Glioblastoma and Other High-grade Gliomas by Real-time PCR, Immunohistochemistry, and <i>In Situ</i> Hybridization Open
Purpose: Reports of cytomegalovirus (CMV) detection in high-grade gliomas (HGG)/glioblastoma have been conflicting. We undertook a comprehensive approach to determine the presence or absence of CMV in tissue, plasma, and serum of HG…
View article: Supplemental Figure 3 from Absence of Cytomegalovirus in Glioblastoma and Other High-grade Gliomas by Real-time PCR, Immunohistochemistry, and <i>In Situ</i> Hybridization
Supplemental Figure 3 from Absence of Cytomegalovirus in Glioblastoma and Other High-grade Gliomas by Real-time PCR, Immunohistochemistry, and <i>In Situ</i> Hybridization Open
IHC controls for tissue immunogenicity. IHC for vimentin (A) shows diffuse positivity and Ki-67 (B) shows widespread nuclear staining in a representative tumor sample. Original magnifications x 200.
View article: Supplemental Table 4 from Absence of Cytomegalovirus in Glioblastoma and Other High-grade Gliomas by Real-time PCR, Immunohistochemistry, and <i>In Situ</i> Hybridization
Supplemental Table 4 from Absence of Cytomegalovirus in Glioblastoma and Other High-grade Gliomas by Real-time PCR, Immunohistochemistry, and <i>In Situ</i> Hybridization Open
Studies that showed presence of CMV in gliomas versus studies that did not.
View article: Supplemental Figure 1 from Absence of Cytomegalovirus in Glioblastoma and Other High-grade Gliomas by Real-time PCR, Immunohistochemistry, and <i>In Situ</i> Hybridization
Supplemental Figure 1 from Absence of Cytomegalovirus in Glioblastoma and Other High-grade Gliomas by Real-time PCR, Immunohistochemistry, and <i>In Situ</i> Hybridization Open
CMV detection by CISH and IHC from CMV negative and positive cells with varying multiplicity of infection (M0I).
View article: Supplemental Tables 1, 2 and 3 from Absence of Cytomegalovirus in Glioblastoma and Other High-grade Gliomas by Real-time PCR, Immunohistochemistry, and <i>In Situ</i> Hybridization
Supplemental Tables 1, 2 and 3 from Absence of Cytomegalovirus in Glioblastoma and Other High-grade Gliomas by Real-time PCR, Immunohistochemistry, and <i>In Situ</i> Hybridization Open
Tables with (1) real-time PCR data of fresh frozen samples, (2) IHC and FISH data of FFPE samples and (3) IHC data of tissue micro arrays.
View article: Supplemental Figure 2 from Absence of Cytomegalovirus in Glioblastoma and Other High-grade Gliomas by Real-time PCR, Immunohistochemistry, and <i>In Situ</i> Hybridization
Supplemental Figure 2 from Absence of Cytomegalovirus in Glioblastoma and Other High-grade Gliomas by Real-time PCR, Immunohistochemistry, and <i>In Situ</i> Hybridization Open
Telomeric DNA detection by CISH. CISH assay targeting human telomeric DNA showing strong nuclear signals (arrows) in tumor cells. Original magnification, x 200.
View article: Supplemental Tables 1, 2 and 3 from Absence of Cytomegalovirus in Glioblastoma and Other High-grade Gliomas by Real-time PCR, Immunohistochemistry, and <i>In Situ</i> Hybridization
Supplemental Tables 1, 2 and 3 from Absence of Cytomegalovirus in Glioblastoma and Other High-grade Gliomas by Real-time PCR, Immunohistochemistry, and <i>In Situ</i> Hybridization Open
Tables with (1) real-time PCR data of fresh frozen samples, (2) IHC and FISH data of FFPE samples and (3) IHC data of tissue micro arrays.
View article: Supplemental Figure 5 from Absence of Cytomegalovirus in Glioblastoma and Other High-grade Gliomas by Real-time PCR, Immunohistochemistry, and <i>In Situ</i> Hybridization
Supplemental Figure 5 from Absence of Cytomegalovirus in Glioblastoma and Other High-grade Gliomas by Real-time PCR, Immunohistochemistry, and <i>In Situ</i> Hybridization Open
Apparent false positive staining in brain tumor. A representative case showing moderate nuclear and cytoplasmic staining for IE1 at a dilution of 1:50. (A) medium power (original magnification x200) and (B) high power (original magnificati…
View article: Supplemental Figure 2 from Absence of Cytomegalovirus in Glioblastoma and Other High-grade Gliomas by Real-time PCR, Immunohistochemistry, and <i>In Situ</i> Hybridization
Supplemental Figure 2 from Absence of Cytomegalovirus in Glioblastoma and Other High-grade Gliomas by Real-time PCR, Immunohistochemistry, and <i>In Situ</i> Hybridization Open
Telomeric DNA detection by CISH. CISH assay targeting human telomeric DNA showing strong nuclear signals (arrows) in tumor cells. Original magnification, x 200.
View article: Supplemental Figure 5 from Absence of Cytomegalovirus in Glioblastoma and Other High-grade Gliomas by Real-time PCR, Immunohistochemistry, and <i>In Situ</i> Hybridization
Supplemental Figure 5 from Absence of Cytomegalovirus in Glioblastoma and Other High-grade Gliomas by Real-time PCR, Immunohistochemistry, and <i>In Situ</i> Hybridization Open
Apparent false positive staining in brain tumor. A representative case showing moderate nuclear and cytoplasmic staining for IE1 at a dilution of 1:50. (A) medium power (original magnification x200) and (B) high power (original magnificati…
View article: Supplemental Figure Legends from Absence of Cytomegalovirus in Glioblastoma and Other High-grade Gliomas by Real-time PCR, Immunohistochemistry, and <i>In Situ</i> Hybridization
Supplemental Figure Legends from Absence of Cytomegalovirus in Glioblastoma and Other High-grade Gliomas by Real-time PCR, Immunohistochemistry, and <i>In Situ</i> Hybridization Open
Figure legends for Supplemental Figures 1-5.
View article: Supplemental Figure Legends from Absence of Cytomegalovirus in Glioblastoma and Other High-grade Gliomas by Real-time PCR, Immunohistochemistry, and <i>In Situ</i> Hybridization
Supplemental Figure Legends from Absence of Cytomegalovirus in Glioblastoma and Other High-grade Gliomas by Real-time PCR, Immunohistochemistry, and <i>In Situ</i> Hybridization Open
Figure legends for Supplemental Figures 1-5.
View article: Supplemental Figure 4 from Absence of Cytomegalovirus in Glioblastoma and Other High-grade Gliomas by Real-time PCR, Immunohistochemistry, and <i>In Situ</i> Hybridization
Supplemental Figure 4 from Absence of Cytomegalovirus in Glioblastoma and Other High-grade Gliomas by Real-time PCR, Immunohistochemistry, and <i>In Situ</i> Hybridization Open
False positive IHC staining in known negative control cells. IHC staining using the IE1 antibody (A). At the working dilution (1:8000) there is no positive staining in uninfected cells (bottom panel). At increased antibody concentrations (…
View article: Comparison of Hepatitis B Virus Infection in HIV-Infected and HIV-Uninfected Participants Enrolled in a Multinational Clinical Trial: HPTN 052
Comparison of Hepatitis B Virus Infection in HIV-Infected and HIV-Uninfected Participants Enrolled in a Multinational Clinical Trial: HPTN 052 Open
Objective: Data comparing hepatitis B virus (HBV) infection in HIV-infected [HIV(+)], and HIV-uninfected [HIV(2)] individuals recruited into the same study are limited. HBV infection status and chronic hepatitis B (cHB) were characterized …
View article: Lgr5-expressing secretory cells form a Wnt inhibitory niche in cartilage critical for chondrocyte identity
Lgr5-expressing secretory cells form a Wnt inhibitory niche in cartilage critical for chondrocyte identity Open
Osteoarthritis is a degenerative joint disease that causes pain, degradation, and dysfunction. Excessive canonical Wnt signaling in osteoarthritis contributes to chondrocyte phenotypic instability and loss of cartilage homeostasis; however…
View article: Lead Time to Recurrence After Posttreatment Plasma and Saliva HPV DNA Testing in Patients With Low-Risk HPV Oropharynx Cancer
Lead Time to Recurrence After Posttreatment Plasma and Saliva HPV DNA Testing in Patients With Low-Risk HPV Oropharynx Cancer Open
Importance Head and neck squamous cell carcinoma is a highly lethal cancer that is often associated with human papillomavirus (HPV). Recent studies have shown promise in the use of HPV DNA detection in salivary rinses and plasma as a facto…
View article: Respiratory Adenovirus Quantification with a Droplet Digital Polymerase Chain Reaction (ddPCR) Assay
Respiratory Adenovirus Quantification with a Droplet Digital Polymerase Chain Reaction (ddPCR) Assay Open
Measuring viral load using droplet digital PCR (ddPCR) is an absolute quantification approach that can facilitate comparability between different laboratories. This approach could prove valuable in studies that focus on the clinical utilit…
View article: Harnessing the Noncanonical Keap1-Nrf2 Pathway for Human Cytomegalovirus Control
Harnessing the Noncanonical Keap1-Nrf2 Pathway for Human Cytomegalovirus Control Open
Antiviral treatment for human cytomegalovirus (HCMV) is limited and suffers from the selection of drug-resistant viruses. Several cellular pathways have been shown to modulate HCMV replication.
View article: Reduced control of SARS-CoV-2 infection is associated with lower mucosal antibody responses in pregnant women
Reduced control of SARS-CoV-2 infection is associated with lower mucosal antibody responses in pregnant women Open
Importance Pregnant women are at increased risk of severe COVID-19, but the contribution of viral RNA load, the presence of infectious virus, and mucosal antibody responses remain understudied. Objective To evaluate the association of COVI…
View article: Enterovirus characterized from cerebrospinal fluid in a cohort from the Eastern United States
Enterovirus characterized from cerebrospinal fluid in a cohort from the Eastern United States Open
EV genomic surveillance is needed for a better understanding of the genetic determinants of neurovirulence. Whole genome sequencing can reveal recombination events missed by traditional molecular surveillance methods.
View article: An increase in enterovirus D68 circulation and viral evolution during a period of increased influenza like illness, The Johns Hopkins Health System, USA, 2022
An increase in enterovirus D68 circulation and viral evolution during a period of increased influenza like illness, The Johns Hopkins Health System, USA, 2022 Open
EV-D68 outbreaks continue to cause influenza like illness that could be overwhelming for the health system due to a significant demand for high flow oxygen. Viral evolution and an increase in the susceptible population are likely driving t…
View article: Impact of Severe Acute Respiratory Syndrome Coronavirus 2 Variants on Inpatient Clinical Outcome
Impact of Severe Acute Respiratory Syndrome Coronavirus 2 Variants on Inpatient Clinical Outcome Open
Background Prior observation has shown differences in COVID-19 hospitalization risk between SARS-CoV-2 variants, but limited information describes hospitalization outcomes. Methods Inpatients with COVID-19 at 5 hospitals in the eastern Uni…
View article: The displacement of the SARS-CoV-2 variant Delta with Omicron: An investigation of hospital admissions and upper respiratory viral loads
The displacement of the SARS-CoV-2 variant Delta with Omicron: An investigation of hospital admissions and upper respiratory viral loads Open
View article: Correction: Absence of Cytomegalovirus in Glioblastoma and Other High-grade Gliomas by Real-time PCR, Immunohistochemistry, and <i>In Situ</i> Hybridization
Correction: Absence of Cytomegalovirus in Glioblastoma and Other High-grade Gliomas by Real-time PCR, Immunohistochemistry, and <i>In Situ</i> Hybridization Open
In the original version of this article (1), the same image was used for Fig. 3A and B. The error has been corrected in the latest online HTML and PDF versions of the article. The authors regret the error.