Marc Freeman
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View article: A dNmnat-sensitized in vivo platform for unbiased discovery of regulators of neurodegeneration
A dNmnat-sensitized in vivo platform for unbiased discovery of regulators of neurodegeneration Open
Axon degeneration drives nervous system dysfunction in diverse neurological diseases and injuries. While key regulators of injury-induced Wallerian degeneration have been identified, approaches have largely relied on axotomy, a particularl…
View article: Comparison of QuPath and HALO platforms for analysis of the tumor microenvironment in prostate cancer
Comparison of QuPath and HALO platforms for analysis of the tumor microenvironment in prostate cancer Open
QuPath, an open-source digital pathology platform, has gained widespread use for image analysis in biomedical research since its release in 2016. However, its reproducibility and reliability compared to commercial software, such as HALO, r…
View article: Glia phagocytose neuronal sphingolipids to infiltrate developing synapses
Glia phagocytose neuronal sphingolipids to infiltrate developing synapses Open
The complex morphologies of mature neurons and glia emerge through profound rearrangements of cell membranes during development. Despite being integral components of these membranes, it is unclear whether lipids might actively sculpt these…
View article: Hypoxia-inducible factor 1 protects neurons from Sarm1-mediated neurodegeneration
Hypoxia-inducible factor 1 protects neurons from Sarm1-mediated neurodegeneration Open
The Sarm1 NAD + hydrolase drives neurodegeneration in many contexts, but how Sarm1 activity is regulated remains poorly defined. Using CRISPR/Cas9 screening, we found loss of VHL suppressed Sarm1-mediated cellular degeneration. VHL normall…
View article: Astrocyte-dependent local neurite pruning in Beat-Va neurons
Astrocyte-dependent local neurite pruning in Beat-Va neurons Open
Developmental neuronal remodeling is extensive and mechanistically diverse across the nervous system. We sought to identify Drosophila pupal neurons that underwent mechanistically new types of neuronal remodeling and describe remodeling Be…
View article: An Ionic Sensor acts in Parallel to dSarm to Promote Neurodegeneration
An Ionic Sensor acts in Parallel to dSarm to Promote Neurodegeneration Open
How neurons to sense when they are terminally dysfunctional and activate neurodegeneration remains poorly defined. The pro-degenerative NAD + hydrolase dSarm/SARM1 can act as a metabolic sensor by detecting pathological changes in NAD + /N…
View article: Adrenergic signaling gates astrocyte responsiveness to neurotransmitters and control of neuronal activity
Adrenergic signaling gates astrocyte responsiveness to neurotransmitters and control of neuronal activity Open
How astrocytes regulate neuronal circuits is a fundamental, unsolved question in neurobiology. Nevertheless, few studies have explored the rules that govern when astrocytes respond to different neurotransmitters in vivo and how they affect…
View article: Developmental and age-related synapse elimination is mediated by glial Croquemort
Developmental and age-related synapse elimination is mediated by glial Croquemort Open
Summary Neurons and glia work together to dynamically regulate neural circuit assembly and maintenance. In this study, we show Drosophila exhibit large-scale synapse formation and elimination as part of normal CNS circuit maturation, and t…
View article: Structural basis of bulk lipid transfer by bridge-like lipid transfer protein LPD-3
Structural basis of bulk lipid transfer by bridge-like lipid transfer protein LPD-3 Open
Bridge-like lipid transport proteins (BLTPs) are an evolutionarily conserved family of proteins that localize to membrane contact sites and are thought to mediate the bulk transfer of lipids from a donor membrane, typically the endoplasmic…
View article: Glial Regulation of Circuit Wiring, Firing, and Expiring in the<i>Drosophila</i>Central Nervous System
Glial Regulation of Circuit Wiring, Firing, and Expiring in the<i>Drosophila</i>Central Nervous System Open
Molecular genetic approaches in small model organisms like Drosophila have helped to elucidate fundamental principles of neuronal cell biology. Much less is understood about glial cells, although interest in using invertebrate preparations…
View article: Astrocyte-dependent local neurite pruning and Hox gene-mediated cell death in Beat-Va neurons
Astrocyte-dependent local neurite pruning and Hox gene-mediated cell death in Beat-Va neurons Open
Neuronal remodeling is extensive and mechanistically diverse across the nervous systems of complex metazoans. To explore circuit refinement mechanisms, we screened for new neuronal subtypes in the Drosophila nervous system that undergo rem…
View article: Tweek-dependent formation of ER-PM contact sites enables astrocyte phagocytic function and remodeling of neurons
Tweek-dependent formation of ER-PM contact sites enables astrocyte phagocytic function and remodeling of neurons Open
Summary Neuronal remodeling generates an enormous amount of cellular debris, which is cleared from the nervous system by glia. At the larva-to-adult transition, Drosophila astrocytes transform into phagocytes and engulf degenerating larval…
View article: TRE1/S1PR1 IS AN EVOLUTIONARILY CONSERVED REGULATOR OF ASTROCYTE DEVELOPMENT
TRE1/S1PR1 IS AN EVOLUTIONARILY CONSERVED REGULATOR OF ASTROCYTE DEVELOPMENT Open
Astrocytes are specialized glial cells that possess an intricate morphology with highly ramified cellular processes interacting closely with individual synapses and other brain cells. Astrocytes perform a range of essential developmental a…
View article: Figure S5 from 27-Hydroxycholesterol Impairs Plasma Membrane Lipid Raft Signaling as Evidenced by Inhibition of IL6–JAK–STAT3 Signaling in Prostate Cancer Cells
Figure S5 from 27-Hydroxycholesterol Impairs Plasma Membrane Lipid Raft Signaling as Evidenced by Inhibition of IL6–JAK–STAT3 Signaling in Prostate Cancer Cells Open
Correlation studies in AR high and AR low patient samples
View article: Figure S7 from 27-Hydroxycholesterol Impairs Plasma Membrane Lipid Raft Signaling as Evidenced by Inhibition of IL6–JAK–STAT3 Signaling in Prostate Cancer Cells
Figure S7 from 27-Hydroxycholesterol Impairs Plasma Membrane Lipid Raft Signaling as Evidenced by Inhibition of IL6–JAK–STAT3 Signaling in Prostate Cancer Cells Open
Scanned uncropped western blot films
View article: Figure S6 from 27-Hydroxycholesterol Impairs Plasma Membrane Lipid Raft Signaling as Evidenced by Inhibition of IL6–JAK–STAT3 Signaling in Prostate Cancer Cells
Figure S6 from 27-Hydroxycholesterol Impairs Plasma Membrane Lipid Raft Signaling as Evidenced by Inhibition of IL6–JAK–STAT3 Signaling in Prostate Cancer Cells Open
Primer Sequences
View article: Supplemental Materials and Methods, Supplementary Tables 1 and 2 from 27-Hydroxycholesterol Impairs Plasma Membrane Lipid Raft Signaling as Evidenced by Inhibition of IL6–JAK–STAT3 Signaling in Prostate Cancer Cells
Supplemental Materials and Methods, Supplementary Tables 1 and 2 from 27-Hydroxycholesterol Impairs Plasma Membrane Lipid Raft Signaling as Evidenced by Inhibition of IL6–JAK–STAT3 Signaling in Prostate Cancer Cells Open
Supplemental Table 1. Adjusted p-Values of SH-4-54 and 27HC (10 uM) single and combination treatment by 2Way ANOVA with Tukey's Multiple Comparisons Posthoc Statistical Analysis Supplemental Table 2. Adjusted p-Values of C188-9 and 27HC (1…
View article: Figure S1 from 27-Hydroxycholesterol Impairs Plasma Membrane Lipid Raft Signaling as Evidenced by Inhibition of IL6–JAK–STAT3 Signaling in Prostate Cancer Cells
Figure S1 from 27-Hydroxycholesterol Impairs Plasma Membrane Lipid Raft Signaling as Evidenced by Inhibition of IL6–JAK–STAT3 Signaling in Prostate Cancer Cells Open
The figures depicts the optimization of PFO-D4 cholesterol probe
View article: Figure S3 from 27-Hydroxycholesterol Impairs Plasma Membrane Lipid Raft Signaling as Evidenced by Inhibition of IL6–JAK–STAT3 Signaling in Prostate Cancer Cells
Figure S3 from 27-Hydroxycholesterol Impairs Plasma Membrane Lipid Raft Signaling as Evidenced by Inhibition of IL6–JAK–STAT3 Signaling in Prostate Cancer Cells Open
PC3 and DU145 dose finding studies for STAT3 inhibitors
View article: Data from 27-Hydroxycholesterol Impairs Plasma Membrane Lipid Raft Signaling as Evidenced by Inhibition of IL6–JAK–STAT3 Signaling in Prostate Cancer Cells
Data from 27-Hydroxycholesterol Impairs Plasma Membrane Lipid Raft Signaling as Evidenced by Inhibition of IL6–JAK–STAT3 Signaling in Prostate Cancer Cells Open
We recently reported that restoring the CYP27A1–27hydroxycholesterol axis had antitumor properties. Thus, we sought to determine the mechanism by which 27HC exerts its anti–prostate cancer effects. As cholesterol is a major component of me…
View article: Data from 27-Hydroxycholesterol Impairs Plasma Membrane Lipid Raft Signaling as Evidenced by Inhibition of IL6–JAK–STAT3 Signaling in Prostate Cancer Cells
Data from 27-Hydroxycholesterol Impairs Plasma Membrane Lipid Raft Signaling as Evidenced by Inhibition of IL6–JAK–STAT3 Signaling in Prostate Cancer Cells Open
We recently reported that restoring the CYP27A1–27hydroxycholesterol axis had antitumor properties. Thus, we sought to determine the mechanism by which 27HC exerts its anti–prostate cancer effects. As cholesterol is a major component of me…
View article: Figure S2 from 27-Hydroxycholesterol Impairs Plasma Membrane Lipid Raft Signaling as Evidenced by Inhibition of IL6–JAK–STAT3 Signaling in Prostate Cancer Cells
Figure S2 from 27-Hydroxycholesterol Impairs Plasma Membrane Lipid Raft Signaling as Evidenced by Inhibition of IL6–JAK–STAT3 Signaling in Prostate Cancer Cells Open
This figure depicts cholesterol clusters after 27HC treatment
View article: Figure S1 from 27-Hydroxycholesterol Impairs Plasma Membrane Lipid Raft Signaling as Evidenced by Inhibition of IL6–JAK–STAT3 Signaling in Prostate Cancer Cells
Figure S1 from 27-Hydroxycholesterol Impairs Plasma Membrane Lipid Raft Signaling as Evidenced by Inhibition of IL6–JAK–STAT3 Signaling in Prostate Cancer Cells Open
The figures depicts the optimization of PFO-D4 cholesterol probe
View article: Figure S4 from 27-Hydroxycholesterol Impairs Plasma Membrane Lipid Raft Signaling as Evidenced by Inhibition of IL6–JAK–STAT3 Signaling in Prostate Cancer Cells
Figure S4 from 27-Hydroxycholesterol Impairs Plasma Membrane Lipid Raft Signaling as Evidenced by Inhibition of IL6–JAK–STAT3 Signaling in Prostate Cancer Cells Open
DU145 27HC effects on Boyden chamber transwell assay
View article: Figure S4 from 27-Hydroxycholesterol Impairs Plasma Membrane Lipid Raft Signaling as Evidenced by Inhibition of IL6–JAK–STAT3 Signaling in Prostate Cancer Cells
Figure S4 from 27-Hydroxycholesterol Impairs Plasma Membrane Lipid Raft Signaling as Evidenced by Inhibition of IL6–JAK–STAT3 Signaling in Prostate Cancer Cells Open
DU145 27HC effects on Boyden chamber transwell assay
View article: Figure S5 from 27-Hydroxycholesterol Impairs Plasma Membrane Lipid Raft Signaling as Evidenced by Inhibition of IL6–JAK–STAT3 Signaling in Prostate Cancer Cells
Figure S5 from 27-Hydroxycholesterol Impairs Plasma Membrane Lipid Raft Signaling as Evidenced by Inhibition of IL6–JAK–STAT3 Signaling in Prostate Cancer Cells Open
Correlation studies in AR high and AR low patient samples